흰쥐 이자 Insulin과 Neuropeptide Y 분비세포의 발생에 관한 면역조직화학적 연구

장인엽,박태우,정윤영,김종중,문정석,김영택,안계훈,김남훈,선희매 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

Cells immunoreactive to insulin(INS-IR) and neuropeptide Y(NPY-IR) were found in prenatal(E15, 17, 19) and postnatal(P0, 5, 10, 15, 30, adult) rat pancreas using immunohistochemistry. Pancreatic INS-IR and NPY-IR were detected by day E17. They were found initially in the interlobular pancreatic duct, intralobular pancreatic duct and pancreatic islet gradually. Especially, INS-IR was detected in the dorsal pancreatic bud by day E19. The INS-IR and NPY-IR patterns were nearly similiar to each other, and many cells revealed colocalization of INS and NPY until day P10. But there was a change between them by the day P15 ; central INS-IR cells and peripheral NPY-IR in the pancreatic islet. The number of pancreatic islets per square millimeter of pancreatic tissue were decreased, and the number of immunoreactive endocrine cells per islet was increased according to development. These results suggest NPY may play a role in the development of the pancreatic endocrine cells.

Feasibility and Effects of a Postoperative Recovery Exercise Program Developed Specifically for Gastric Cancer Patients (PREP-GC) Undergoing Minimally Invasive Gastrectomy

Cho, In,Son, Younsun,Song, Sejong,Bae, Yoon Jung,Kim, Youn Nam,Kim, Hyoung-Il,Lee, Dae Taek,Hyung, Woo Jin The Korean Gastric Cancer Association 2018 Journal of gastric cancer Vol.18 No.2

Purpose: Exercise intervention after surgery has been found to improve physical fitness and quality of life (QOL). The purpose of this study was to investigate the feasibility and effects of a postoperative recovery exercise program developed specifically for gastric cancer patients (PREP-GC) undergoing minimally invasive gastrectomy. Materials and Methods: Twenty-four patients treated surgically for early gastric cancer were enrolled in the PREP-GC. The exercise program comprised sessions of In-hospital Exercise (1 week), Home Exercise (1 week), and Fitness Improvement Exercise (8 weeks). Adherence and compliance to PREP-GC were evaluated. In addition, body composition, physical fitness, and QOL were assessed during the preoperative period, after the postoperative recovery (2 weeks after surgery), and upon completing the PREP-GC (10 weeks after surgery). Results: Of the 24 enrolled patients, 20 completed the study without any adverse events related to the PREP-GC. Adherence and compliance rates to the Fitness Improvement Exercise were 79.4% and 99.4%, respectively. Upon completing the PREP-GC, patients also exhibited restored cardiopulmonary function and muscular strength, with improved muscular endurance and flexibility (P<0.05). Compared to those in the preoperative period, no differences were found in symptom scale scores measured using the European Organization for Research and Treatment of Cancer (EORTC) Core Quality of Life Questionnaire (QLQ-C30) and Quality of Life Questionnaire-Stomach Cancer-Specific Module (QLQ-STO22); however, higher scores for global health status and emotional functioning were observed after completing the PREP-GC (P<0.05). Conclusions: In gastric cancer patients undergoing minimally invasive gastrectomy, PREP-GC was found to be feasible and safe, with high adherence and compliance. Although randomized studies evaluating the benefits of exercise intervention during postoperative recovery are needed, surgeons should encourage patients to participate in systematic exercise intervention programs in the early postoperative period (Registered at the ClinicalTrials.gov, NCT01751880).

월성원자력발전소 주변의 삼림식생 분석

이호준,김인택,정흥락,전영문,윤영진,임웅규 建國大學校基礎科學硏究所 1996 理學論集 Vol.21 No.-

월성원자력발전소 주변의 삼림식생을 분석하기 위하여 1994년 4월부터 1994년 10월까지 식물사회학적 조사를 실시하였다. 또한 상관에 의해 현존식생도 및 녹지자연도를 작성하였으며, 순 1차 생산력과 식물현존량도 추정하였다. 군집조성표에 의한 삼림군락은 소나무군락, 곰솔군락, 굴참나무군락, 상수리 나무군락의 4군락으로 구분되었다. 각 군락의 주요 구성종으로는 소나무군락에서 소나무, 졸참나무, 털진달래, 맑은대쑥, 산거울, 곰솔군락에서 곰솔, 소나무, 진달래, 청미래덩굴, 굴참나무군락에서 굴참나무, 조록싸리, 큰기름새, 맑은대쑥, 댕댕이덩굴, 상수리나무군락에서 상수리나무, 갈참나무, 큼기름새, 그늘사초, 맑은대쑥 등이다. 현존식생도에 의한 삼림군락의 분포율은 곰솔군락이 40.3%로 가장 높고, 녹지자연도는 7등급이 60.0%로 가장 넓은 분포율을 보였다. 삼림토양의 pH는 4.45∼5.32, 유기물함량은 5.61∼6.34%의 범위로 나타났다. Montreal model에 의한 순 1차 생산력은 1,555.2g/㎡/yr, 식물현존량은 14,963.32ton/㎢로 추정되었다 The forest vegetation, estimation of phytomass and net primary production on the vicinity of Wolsong Atomic Power Plant were analyzed on the basis of the phytosociological data. And depending on physiognomy, actual vegetation map and the degree of green naturality(DGN) was drawn in 1:50,000 scale. According to Z-M method, the forest vegetation was classified into 4 communities; A. Pinus densiflora community, B. Pinus thunbergii community, C. Quercus variabilis community, D. Quercus acutissima community. Generally, Pinus densiflora, Quercus variabilis, in tree-1 layer, Quercus acutissima, Quercus variabilis, in tree-2 layer, Rhodedendron mucronulatum, Quercus serrate, in shrub layer, Artemisia keiskeana, Carex humilis, in herb layer, showed high coverage index. The pH and contents of organic matter of the forest soil collected in each sites were shown range of 4.45 to 5.32 and 5.61% to 6.34% in mean of communities respectively. The distribution rate in actual vegetation map were the highest in Pinus thunbergii community. The average DGN showed to 5.1. Net primary production and phytomass per unit area which estimated by Montreal model were assumed in 1,555.2g/㎡/yr, 14,963.32ton/㎢, respectively.

제2형 당뇨병 남성 환자에서 유리 테스토스테론과 성호르몬 결합 글로블린 농도

남기덕,김영설,박철영,오승준,김덕윤,우정택,김성운,양인명,김진우,최영길 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.24 No.6

연구배경:인슐린저항성은 제2형 당뇨병과 심혈관질환의 주요한 위험 인자로 성호르몬과 상호 관계가 있다고 알려져 있다. 그러나 여성과는 달리 남성에서는 연령과 인슐린저항성에 따른 유리 테스토스테론과 성호르몬 결합 글로블린 농도의 변화에 대한 연구가 부족한 실정이다. 그러므로, 본 저자등은 제2형 당뇨병 남성 환자에서 정산인과 비교해서 유리 테스토스테론과 성호르몬 결합 글로블린 농도를 측정하고 연령에 따른 변화 정도를 알아보고자 하였다. 방법:대상 환자 모두에서 연령과 체질량지수, 총 콜레스테롤, 중성지방, 공복혈당과 인슐린 농도를 측정하였다. 혈중 유리 테스토스테론 농도는 방사면역측정법(radioimmunoassay)을 이용해서 측정하였고, 혈중 성호르몬 결합글로불린은 면역방사계측측정법(immunoradiometric assay)을 이용해서 측정하였다(Diagnostic System Laoratories, Wbster, TX, USA). 결과:1)제2형 당뇨병 남성 환자에서 정상 대조군 남성에 비해 성호르몬 결합글로불린은 104.1±35.0 vs 25.7±3.5 mole×10??로 의미 있게 높았으나(p<0.001), 유리 테스토스테론은 13.7±9.5 vs 13.6±6.5 ng/dL로 차이가 없었다. 2)연령과 성호르몬 결합 글로블린 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.40로 중등고의 양의 상관 관계를 보였고(p<0.001), 정상 대조군 남성에서 0.11로 유의한 상관 관계를 보이지 않았다. 연령과 유리 테스토스테론 사이의 상관 계수는 제2형 당뇨병 남성 환자에서 0.08, 정상 대조군 남성에서-0.17로 모두에서 유의한 상관 관계를 보이지 않았다. 3)연령과 체질량지수를 보정한 후에 제2형 당뇨병 남성 환자와 정상 대조군 남성에서 혈중 인슐린 농도, 유리 테스토스테론과 성호르몬 결합 글로블린 사이에는 상관 관계가 없었다. 결론:제2형 당뇨병 남성 환자에서 정상 남성에 비해 성호르몬 결합 글로블린 농도가 증가되어 있었으며, 유리 테스토스테론은 차이가 없었다. 연령이 증가함에 따라 성호르몬 결합 글로블린이 제2형 당뇨병 남성 환자에서 정상 남성에 비해 증가 폭이 의미있게 컸으며, 유리 테스토스테론은 변화가 없었다. Background: Insulin resistance is a risk factor for cardiovascular disease and type 2 diabetes mellitus. There are many previous studies indicating that insulin lowers serum sex hormone-binding globulin levels, and there is inverse correlation between insulin resistance and serum sex hormone-binding globulin levels in women. However, in men, a limited number of studies are available to explain the effect of sex hormone on age and insulin. Therefore, the present study was undertaken to investigate the relationship among free testosterone, hormone-binding globulin and age in type 2 diabetic men and control subjects. Method: Age, body mass index, total cholesterol, triglyceride, fasting blood sugar, and insulin concentrations were examined on 89 type 2 diabetic men and 47 control subjects. The free testosterone level was measured by commercially available double-antibody system (Radioimmunoassay). The sex hormone-binding globulin level was also measured by commercially available double-antibody system(Immunoradiometric assay). Results: 1) Sex hormone-binding globulin level was significantly increased in patients with type 2 diabetes. However, there was no significantly difference in free testosterone level between the two groups. 2) Sex hormone-binding globulin was positively correlated with age (r=0.4, p <0.001) in patients with type 2 diabetes. Sex hormone-binding globulin and free testosterone were not correlated with age in control sujects. 3) Free testosterone and sex hormone-binding globulin concentrations were not significantly related to serum insulin concentration after adjusting for age and body mass index. Conclusions: We observed increased sex hormone-binding globulin concentration in diabetes man, and was a positively related to age. Further studies are needed to understand the relationships between age, insulin resistance, testosterone, and sex hormone-binding globulin concentrations(J Kor Diabetes Asso 24:699~707,2000).

합천군 정양지와 박실지의 곤충 및 저서성 대형무척추동물의 분포

정선우,전세근,김인택,박정원,윤춘식 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

The community structures of benthic macro-invertebrates and insects of reservoirs Jeongyang and Pakasil in Hapcheon-gun were studied. The surveyed sites were three streams and waterfronts of each lake. The Collection was performed from April to October of 2005. As a result, total macreo-invertebrates collected in the reservoir Jeongyang were 3 phyla, 4 classes, 12 orders, 29 families and 56 species. In insects, 12 orders, 71 families and 167 species were identified. Total macro-invertebrates collectd in the reservoir Paksil were 2 phyla, 3 classes, 8 orders, 19 families and 36 species. In insects, 11orders, 50 families and 137 species were identified.Dominant species of benthic macro-invertebrates and insects in reservoir Jeongyang were Chiromomus sp and Nysius plebejus respectively. Dominant species of benthic macro-invertebrates and insects in reservoir Paksil were Physella acuta and Nysius plebejus respectively. The total species richness and species diversity of benthic macro-invertebrates in the reservoir Jeongyang was 16.88 and 1.43 respectively. The total species richness and species diversidty of benthic macro-invertebrates in the reservoir Paksil was 12.12 and 1.10 respectively. The total species richness and species diversity of insects in the reservoir Jeongyang was 56.65 and 1.99 respectively. The total species richness and species diversity of benthic macro-invertebrates in the reservoir Paksil was 47.65. and 0.90 respectively.

흰쥐 올리브소뇌계통의 발생에 따른 IGF-I에 대한 면역조직화학적 연구

장인엽,윤상필,정윤영,김종중,문정석,김영택,장영훈 대한해부학회 1999 Anatomy & Cell Biology Vol.32 No.4

흰쥐 뇌에서 Tyrosine Hydroxylase분비세포의 출현시기 및 분포에 관한 면역조직화학적 연구

정윤영,선희매,김영택,김남훈,안계훈,장인엽,김종중,문정석,강양수 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.1

The immunohistochemical (ABC method) studies on the ontogeny and localization of the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) were examined in rat brain from the 12th fetal day until the 9th postnatal day. The results obtained were as follows : 1. Tyrosine hydroxylase was first detected immunohistochemically at embryonic day 13(E13). At this stage, the TH-containing neurons were still migrating and cytologically immature. 2. The changes occuring from the early to the late prenatal stages of development appeared to be the result of an increase in the number and size of the TH-containing cells and in the length of nerve fibers. 3. The distribution of the TH-containing neurons at embryonic day 19(E19) more closely resembled catecholaminergic neurons in the adult rat brain : 1) a rhombencephalic group became the A1-7 ; 2) a ventral mesencephalic group became A8-10 ; 3) a ventral prosencephalic group and a few neurons ventral to the striatum became A11-14 ; and 4) the cells of the olfactory bulb corresponded to the A15. 4. The cytodifferentiation of the TH-immunoreactive neurons was accompanied by continued migration to form the collective catecholaminergic groups during ontogenesis. In addition, the morphology of the TH-immunoreactive neurons in the late prenatal stage resembled in the adult rat brain.

폐경기 증후군 환자에서 Cyclofenil의 치료 효과

김덕윤,고은미,우정택,양인명,김진우,김영설,김광원,최영길 최신의학사 1992 最新醫學 Vol.35 No.10

개 이자의 Somatostatin 양성세포의 발달에 관한 면역조직화학적 연구

장인엽,윤상필,정윤영,김종중,문정석,한길현,김영택,안계훈,선희매 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

Cells immnunoreactive for somatostatin(SOM) and glucagon(GLU) were investigated in postnatal day 0, 7, 14, 28, 90, 180 and adult canine pancreas using light microscopic immunohistochemistry. Pancreatic SOM-immunoreactive cells were found in pancreatic acini, pancreatic duct, and pancreatic islet gradually. Pancreatic SOM-immunoreactive cells were more located in pancreatic parenchyme than pancreatic islet throughout pancreas by postnatal day 28. But there was change by the postnatal 3 months : pancreatic SOM-immunoreactive cells were more located in pancreatic islet than acini and duct. It is suggested that the number of the SOM-immunoreactive cells may not increase with age apparently. A small number of SOM-immunoreactive cells that showed GLU-immunoreactivity were also observed in canine pancreatic islet. The physiologic consequences of these changes remain to be determined. These results sugegest that SOM in the developing pancreas may relate to the secretion of pancreatic hormones.

취서산과 천성산 수계의 저서성 대형무척추동물 군집구조

정선우,김인택,박중석,이종남,이동준,윤춘식 7개 국립대학교 환경연구논문집 공동발행 위원회 2005 공업기술연구 Vol.5 No.-

The community structures of benthic macroinvertebrates in the streams of Mt. Kauisan and Mt. Chuiseosan were studied. The surveyed sites were streams of Mt. Chuiseosan area, Tongdosa area and Naewonsa area of Mt Chunsungsan. The collection was performed from May to October of 2005. As a result, total macroinvertbrates collected in the stream of Mt Chuiseosan area were 3 phyla, 3 classes, 10 orders, 19 families, 31 species and 293 individuals. From the Tongdosa area, 2 phyla, 3 classes, 11 orders, 33 families, 51 species and 827 individuals were identified. From the Naewonsa area, 3 phyla, 4 classes, 11orders, 23 families, 32 species and 457 individuals were found. The community analysis showed the highest species diversity and species richness in Tongdosa area than other areas. The dominant species of this study was Semisulcospira libertina and it occupied 21.66% of total individuals.