복합 저수지군의 자기유역 유입량 산정방법

유주환 永同大學校 2001 硏究論叢 Vol.7 No.1

강우에 계절적 변동이 크고 유역면적이 작아 수자원 확보에 불리한 환경을 가진 우리나라는 용수수요의 증가에 따라 댐을 증축하여 수자원을 확보하고 있는 실정이다. 이 때문에 형성된 복합 저수지군의 규모와 관리의 중요성은 점차 커져왔다. 이때 저수지 유입량을 산정하는 것은 수자원 관리의 기본이 된다. 특히 복합 저수지군의 각댐 유입량에는 상류댐의 유입량이 포함되어 있지만 수자원의 효율적 관리를 위한 시스템 분석 등에 자기유역 유입량의 산정이 기초가 된다. 이에 본 연구에서는 상류댐 유입량을 포함한 댐 관측 유입량으로부터 각 댐별 자기유역 유입량을 산정하는 방법을 제시하고 수계내 저수지별 유입량을 산정하고 그 적정성을 검증하였다. In our country which has the inferiority that the rainfalls in its area are much fluctuated due to the seasons and its drainage area is small the water resources has been stored by means of making additional dams as water demands increase. And the importance of the scale and management of multiple reservoir system becomes larger. So the calculation of the reservoir inflows is fundamental for the management of the water resources. Especially the calculation of the individual lateral inflow into each dam of the multiple system is basic on the system analysis for the effective management of water resources. Therefore in this study the methodology which can calculate the amount of the lateral inflow into individual dam using the registered dam inflow data involving the upstream dam inflow was suggested and its fitness was verified.

Serratia marcescens에서 cAMP receptor protein(CRP)유전자의 클로닝 및 해석

유주순,문종환,정수열,김혜선,최용락 東亞大學校附設遺傳工學硏究所 1999 遺傳工學硏究 Vol.- No.6

전사조절인자로서 잘 알려져 있는 cAMP receptor protein(CRP)은 cAMP와 DNA에 결합하는 특별한 활성을 가지고 있으며, cAMP-CRP complex를 형성하여 수많은 유전자의 발현조절에 관여한다. 이러한 측면에서 cAMP-CRP의 조절은 어떤 면에서 총체적 조절체계라고까지 한다. 본 연구는 Serratia 균주에서 crp유전자의 분자적 특성 및 cAMP에 의한 발현조절을 받는 분자기구를 해석하고자 유전자를 클로닝하고 발현을 확인하였다. MacConkey배지에서 maltose를 탄소원으로 충분히 이용하지 못하는 대장균 TP2139(△crp,△lac)를 숙주로 이용하고, 염색체DNA를 library로 작성하여 얻은 형질전환체 약 일만개의 콜로니에서 red colony를 나타내는 5종류 의 양성 클론을 얻었다. 이들 클론을 Southern방법으로 확인한 결과 3kb의 단편을 가진 pCKB12클론이 crp유전자를 coding하고 있음을 확인하였다. glpD-lacZ 융합 plasmid인 pLDC6 의 BamHI부위에 pCKB12의 3kb 단편을 삽입시킨 재조합 plasmid pLDC 6-Scrp를 작성하여, 클로닝된 Serratia 의 crp 유전자가 대장균에서 유전자 전사조절에 미치는 영향을 확인한 결과 cAMP-CRP 복합체 형성에 의한 전사조절 기능이 확인되어졌다. One of the better-characterized transcription factor of E. coli is the cAMP receptor protein(CRP) and the CRP binds cAMP and DNA. The cAMP-CRP complex is involved in regulation of many genes at bacteria. The cAMP-CRP regulatory element represents, in some respects, a global regulatory network. The aim of this work was to study the structure and the mechanisms controlling the expression of CRP in Serratia marcescens. We have been get 5 different clones from Serratia which stimulated the cells to use maltose as a sole carbon source in E. coli TP2139. The crp gene clone, pCKB12, was confirmed by Southern hybridization with E. coli crp gene. The location of the crp gene was determined by constructing subclones carrying various portions of pCKB12. To investigate the potential role of CRP in E. cloi, lacZ fused plasmids were constructed and investigated the β-galactosidase activity of the fused plasmid. The Serratia marcescens cAMP receptor protein can substitute the E. coli CRP in transcriptional activation at the lacZ gene. These results suggest that Serratia marcescens cAMP receptor protein complex functions to regulate several promoters in E. coli.

전력 시장 변화를 고려한 대청 수력 발전소의 최적 공급량 결정

유주환 永同大學校 2000 硏究論叢 Vol.6 No.1

현재 수력 발전량은 국영 기업체에 독점적으로 수매되고 있으나 수매 기관이 민영화, 다기화될 경우 다자간의 시장경제 체제가 형성됨에 따라 수력발전 생산자는 최적 공급량과 공급의 신뢰도 수준의 제시가 판매상 요구된다. 이에 본 연구에서는 대청다목적 댐의 수력 발전소를 대상으로 동적계획 기법과 황금비 조사 기법을 이용한 본 모형을 적용하여 유입량의 신뢰도와 수력 발전 공급량의 최적치를 결정하였다. In the present, hydro-power energy has been monopolizedly sold a government corporation. In case the corporation becomes under private management and there are more private corporations so that the market of the hydro-power energy may be in free competition the producer of the energy will require the optimal supply and the reliability of the supply for a deal. In this study, applying the model including the dynamic programming and the golden ratio search method to the power system of Daechung multipurpose dam the optimal values of the reliabilities of inflows and the power supply were determined.

Poly(ρ-phenylenesulfide)와 Poly(ρ-phenylene)으로부터 제조된 탄소의 리튬 이온 2차전지 anode 재료로서의 전기화학적 특성

유덕영,이주성,박수길,변지형,류신환,정윤이 한양대학교 에너지·환경기술연구소 1998 에너지·環境技術論文集 Vol.4 No.-

Poly(p-phenylenesulfide)와 poly(p-phenylene)으로부터 제조된 탄소를 리튬이온 2차전지용 음극재료로 사용하여 전기화학적특성을 연구하였다. 이들 고분자들을 질소 분위기하에서 승온속도 2℃/min로 1000℃까지 상승시킨 후, 1시간 동안 탄화시켜 탄소를 제조하였다. Poly(p-phenylene)으로부터 제조된 탄소가 보다 더 규칙적인 구조를 가지기 때문에 Poly(p-phenylenesulfide)으로부터 제조된 탄소보다 더 많은 용량과 충방전 효율을 나타내었다. 수산화리튬과 염화주석(Ⅱ)을 첨가하여 용량의 증가와 충방전 효율의 증가를 가져올 수 있었다. 이중에서 poly(p-phenylene)에 염화주석(Ⅱ)을 첨가시켜 제조된 탄소가 가장 큰 충방전 용량과 충방전 효율의 향상을 가져왔다. Carbon inaterials manufactured from poly(p-phenylene sulfide) and poly(p-phenylene), were studied on electrochemical characteristics as anode materials for lithium ion secondary battery. These polymer precursors were heat treated for 1hr at 1000℃ with the rate 2℃/min under nitrogen atmosphere. Carbon manufactured from poly(p-phenylene) showed higher capacity and coulomb efficiency of charge/discharge than carbon from poly(p-phenylene sulfide) because the former has better ordered structure. Carbon manufactured from polymer precursors adding stannous chloride or lithium hydroxide showed higher capacity and better efficiency of charge/discharge. Also, carbon manufactured by adding stannous chloride to poly(p-phenylene) showed the highest capacity and efficiency of charge/discharge.

蒼附導痰丸이 白鼠의 排卵에 미치는 影響

강복환,박종문,박경미,조한백,엄주오,유심근 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

Changbudodamhwan(CBH) is used in female infertility caused by ovulation disorder. An attempt was made to evaluate the influences of CBH on the serum concentrations of FSH, LH, estradiol(E_(2)) and progesterone, the histological and optical changes of ovary, ovary weight of rats. The results of the study were as follows : 1. Blood FSH level increased experimental group as compared with control group, which showed no efficacy. 2. Blood LH level increased in experimental group as compared with control group, which showed no efficacy. 3. Blood E_(2) level increased in experimental group as compared with control group, which showed no efficacy. 4. Blood progesterone level increased in experimental group as compared with control group, which showed no efficacy. 5. In optically observations of ovary, weight of ovary increased in experimental group as compared with control group, which showed no efficacy. 6. In histological observations of ovary, ovulation increased in experimental group as compared control group, which showed efficacy. According to these results, CBH influence on the ovulation and ovary of rats.

Plasmid DNA pBR 322의 증폭과 Hydroxyapatite Chromatography에 의한 분리

오두환,유주현,신원철,정건섭,유승구 연세대학교 산업기술연구소 1984 논문집 Vol.16 No.1

The amplification and the isolation conditions of pBR322 plasmid DNA were investigated. For the amplification of pBR322 plasmid DNA, cells from logarithmic growth phase were most effective. The optimal conditions for the amplification of pBR322 plasmid DNA in Escherichia coli GM4 were obtained as follows; 50-150 ㎍/ml of chloramphenicol concentration, 6-8 hours of incubation time in the presence of chloramphenicol. Ampicillin and tetracycline had no effect on the amplification of pBR322 plasmid DNA. Approximately 1 mg of pBR322 plasmid DNA was purified from 3.5 ℓof Escherichia coli GM4 culture broth by hydroxyapatite chromatography. The purified pBR322 plasmid DNA was expressed in Escherichia coli C600.

Systemic Lupus Erythematosus를 동반한 Multiple sclerosis

이지현,이기주,윤성호,부귀범,손현화,박유환,정춘해,김진호,정원영 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

Multiple sclerosis and lupus erythematosus are chronic, potentially disabling diseases of unknown cause. It is a rare occasion that the finding of both diseases occurs in one patient. Because both diseases may affect the central nervous system, it may be difficult at times to differentiate symptoms of lupus erythematosus from those of multiple sclerosis. As far as we know, there was no case report of SLE in multilple sclerosis in Korea. We report a case of multiple sclerosis accompanied by SLE.

Optimal distance exponent of inverse distance method

Yoo Ju-Hwan 한국수자원학회 2018 한국수자원학회논문집 Vol.51 No.5

역거리법에 포함된 지수 값을 제곱으로 고정하지 않고 변수로 취급하여 강수량 자료를 바탕으로 지수 값의 최적치를 산출하였다. 이를 위해서 한강 상류부, 한강 하류부, 금강 상류부, 낙동강 중류부 등 4개 Group으로 나누고 각 Group 내 7개 관측소에 대하여 총 52개의 Case를 분석하였다. 각 Group에서 기준 관측소 1개와 주변관측소 4개를 조합한 Case별로 거리 지수 값의 최적치를 구하였다. 이 최적치를 산출하기 위해서 황금비 분할 조사법을 적용하였고 강수 자료는 10년(2004~2013년) 간의 시우량 자료를 사용하였다. 이와 같이 구한 최적치를 최근 3년(2014~2016년) 간에 대하여 검증하였다. 본 연구에서 구한 최적의 거리 지수 값은 4개 Group에서 평균적으로 각각 3.280, 1.839, 2.181, 2.005로 나타났고 전체 평균하면 2.326이었다. 그리고 최적의 지수 값을 적용한 역거리법은 지수 값을 제곱으로 한 기존 역거리법과 비교하여 우수함을 보였다. We calculated the optimal exponent values based on the hourly rainfall data observed in South Korea by treating the exponent value as a variable without fixing it as a square in the inverse distance method. For this purpose, rainfall observation stations providing the data are classified into four groups which are located at the Han river upstream, downstream, the Geum river upstream, and the Nakdong river midstream area. A total of 52 cases were analyzed for seven stations in each group. The optimal exponent value of distance was calculated in a case including one base station and four surrounding stations in a group. We applied the golden section search method to calculating this optimum values using rainfall data for 10 years (2004~2013) and verified the optimum values for the last three years (2014~2016). We compared and analyzed two results of the conventional inverse distance method and the inverse distance method in this study. The optimal values of distance exponent obtained in this study were 3.280, 1.839, 2.181, and 2.005 respectively, in the four groups, and totally mean value was 2.326. It is shown the proposed inverse distance method applying the optimal exponent is superior to the conventional inverse distance method.

Prediction of golden time using SVR for recovering SIS under severe accidents

Yoo, Kwae Hwan,Back, Ju Hyun,Na, Man Gyun,Kim, Jae Hwan,Hur, Seop,Kim, Chang Hwoi Elsevier 2016 Annals of nuclear energy Vol.94 No.-

<P><B>Abstract</B></P> <P>Nuclear power plants (NPPs) are designed in consideration of design basis accidents (DBAs). However, if the safety injection system (SIS) is not working properly in a loss-of-coolant-accident (LOCA) situation, it can induce a severe accident that exceeds DBAs. Therefore, it is important to properly actuate the SIS before a DBA becomes a severe accident. If the SIS is not working in time, the reactor core may be uncovered and the reactor vessel (RV) may be damaged. In this paper, we defined the golden time as the available time from an initial SIS malfunction for actuating the SIS to prevent reactor core uncovery and RV failure. A support vector regression (SVR) model was applied to predict the golden time. The input variables and parameters of the SVR model were selected and optimized by using a genetic algorithm. The data set of severe accident scenarios was obtained by using the Modular Accident Analysis Program (MAAP) code. An optimized power reactor (OPR1000) was used for the simulations. It was shown that that the proposed SVR model could predict the golden time accurately.</P> <P><B>Highlights</B></P> <P> <UL> <LI> If the safety injection system is not working in time, the reactor vessel may be damaged. </LI> <LI> It is important to properly actuate the SIS before a DBA becomes a severe accident. </LI> <LI> Golden time is defined as the available time from an initial SIS malfunction for recovering SIS. </LI> <LI> A support vector regression (SVR) model was applied to predict the golden time. </LI> <LI> It was shown that the proposed SVR model could predict the golden time accurately. </LI> </UL> </P>

Hexokinase-II Inhibition Synergistically Augments the Anti-tumor Efficacy of Sorafenib in Hepatocellular Carcinoma

Yoo, Jeong-Ju,Yu, Su Jong,Na, Juri,Kim, Kyungmin,Cho, Young Youn,Lee, Yun Bin,Cho, Eun Ju,Lee, Jeong-Hoon,Kim, Yoon Jun,Youn, Hyewon,Yoon, Jung-Hwan MDPI 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.6

<P>This study aimed to examine whether inhibition of hexokinase (HK)-II activity enhances the efficacy of sorafenib in in-vivo models of hepatocellular carcinoma (HCC), and to evaluate the prognostic implication of HK-II expression in patients with HCC. We used 3-bromopyruvate (3-BP), a HK-II inhibitor to target HK-II. The human HCC cell line was tested as both subcutaneous and orthotopic tumor xenograft models in BALB/c nu/nu mice. The prognostic role of HK-II was evaluated in data from HCC patients in The Cancer Genome Atlas (TCGA) database and validated in patients treated with sorafenib. Quantitative real-time PCR, western blot analysis, and immunohistochemical staining revealed that HK-II expression is upregulated in the presence of sorafenib. Further analysis of the endoplasmic reticulum-stress network model in two different murine HCC models showed that the introduction of additional stress by 3-BP treatment synergistically increased the in vivo/vitro efficacy of sorafenib. We found that HCC patients with increased HK-II expression in the TCGA database showed poor overall survival, and also confirmed similar results for TCGA database HCC patients who had undergone sorafenib treatment. These results suggest that HK-II is a promising therapeutic target to enhance the efficacy of sorafenib and that HK-II expression might be a prognostic factor in HCC.</P>