Dietary Supplementation with Acanthopanax senticosus Extract Modulates Gut Microflora in Weaned Piglets

Yin, Fugui,Yin, Yulong,Kong, Xiangfeng,Liu, Yulan,He, Qinghua,Li, Tiejun,Huang, Ruilin,Hou, Yongqing,Shu, Xugang,Tan, Liangxi,Chen, Lixiang,Gong, Jianhua,Kim, Sung Woo,Wu, Guoyao Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.9

This study was conducted to investigate the effects of Acanthopanax senticosus extract (ASE) as a dietary additive on gut microflora in weaned piglets. A total of sixty pigs were weaned at 21 d of age (BW = $5.64{\pm}0.23kg$) and allocated on the basis of BW and litter to three dietary treatments in a randomized complete block design. The dietary treatments were: control group (basal diet), antibiotics group (basal diet+0.02% colistin), and ASE group (basal diet+0.1% ASE). On d 7, 14 and 28 after consuming the experimental diets, five piglets per group were sacrificed and then the contents from the jejunum, ileum and cecum were collected to determine changes in the microbial community by using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique and estimating the contents of Lactobacillus and E. coli by in vitro culturing methods. The results showed that the ASE promoted the microflora diversity in the cecum. Enumeration of bacteria in the gut contents showed that the number of Lactobacillus increased (p<0.05), while that of E. coli decreased (p<0.05) when compared with the other 2 groups as the days of age progressed post-weaning. These findings suggested that the ASE, as a substitute for dietary antimicrobial products, could improve the development of the normal gut microflora and suppress bacterial pathogens, and effectively promote a healthy intestinal environment.

Microstructural Evolution of T91, T92 and X20 Steel Used as Superheater and Reheater in Power Plants (초)

He Yin Sheng,Jung Chel Chang,Sung Ho Lee,Ji Ling Dong,Dae Hwang Yoo,Kee Sam Shin 대한금속ㆍ재료학회 2009 대한금속재료학회 학술대회 개요집 Vol.2009 No.-

Energy Efficiency of Distributed Massive MIMO Systems

He, Chunlong,Yin, Jiajia,He, Yejun,Huang, Min,Zhao, Bo The Korea Institute of Information and Commucation 2016 Journal of communications and networks Vol.18 No.4

In this paper, we investigate energy efficiency (EE) of the traditional co-located and the distributed massive multiple-input multiple-output (MIMO) systems. First, we derive an approximate EE expression for both the idealistic and the realistic power consumption models. Then an optimal energy-efficient remote access unit (RAU) selection algorithm based on the distance between the mobile stations (MSs) and the RAUs are developed to maximize the EE for the downlink distributed massive MIMO systems under the realistic power consumption model. Numerical results show that the EE of the distributed massive MIMO systems is larger than the co-located massive MIMO systems under both the idealistic and realistic power consumption models, and the optimal EE can be obtained by the developed energy-efficient RAU selection algorithm.

Energy Efficiency of Distributed Massive MIMO Systems

Chunlong He,Jiajia Yin,Yejun He,Min Huang,Bo Zhao 한국통신학회 2016 Journal of communications and networks Vol.18 No.4

In this paper, we investigate energy efficiency (EE) of thetraditional co-located and the distributed massive multiple-inputmultiple-output (MIMO) systems. First, we derive an approximateEE expression for both the idealistic and the realistic power consumptionmodels. Then an optimal energy-efficient remote accessunit (RAU) selection algorithm based on the distance between themobile stations (MSs) and the RAUs are developed to maximizethe EE for the downlink distributedmassive MIMO systems underthe realistic power consumption model. Numerical results showthat the EE of the distributedmassiveMIMOsystems is larger thanthe co-locatedmassiveMIMOsystems under both the idealistic andrealistic power consumptionmodels, and the optimal EE can be obtainedby the developed energy-efficient RAU selection algorithm.

Research on Modeling Improvement of Rubber Isolators by Considering Multi-loading Conditions

Yin-Yan Zhao,Wen-Bin Shangguan,Nong Cheng,Xiao-Jing He 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

The stress-strain relationships of a rubber material in uniaxial tension, equi-biaxial tension and plane shear loading conditions are tested here. Constitutive parameters in different constitutive models, such as Mooney-Rivlin model, Van Der Waals model and Marlow model can be obtained by least square method using measured and calculated stress-strain relations. The rubber isolators in two rubber mounts are investigated here. Rubber mount I bears stretching/compression deformation or shear deformation, while mount II bears both deformations simultaneously. Using obtained constitutive constants, the rubber isolators’ static stiffness values in three mutually perpendicular directions are calculated, analyzed and compared with their measured results. Based on Mooney-Rivlin model, the effects of these constitutive constants on static stiffness of the two mounts are discussed by comparison. The effects of different models on static force-displacement characteristics of rubber mount I are also analyzed. The conclusion is that it is necessary to choose a relatively accurate constitutive model and appropriate methods for obtaining the model constants in describing rubber characteristic.

Heteromerization of μ-opioid receptor and cholecystokinin B receptor through the third transmembrane domain of the μ-opioid receptor contributes to the anti-opioid effects of cholecystokinin octapeptide

Yin Yang,Qian Li,Qi-Hua He,Ji-Sheng Han,Li Su,You Wan 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Activation of the cholecystokinin type B receptor (CCKBR) by cholecystokinin octapeptide (CCK-8) inhibits opioid analgesia. Chronic opiate treatment leads to an increase in the CCK-8 concentration and thus enhances the antagonism of CCK-8 against opioid analgesia; the underlying molecular mechanisms remain of great interest. In the present study, we validated the colocalization of the μ-opioid receptor (MOR) and CCKBR in pain signal transmissionrelated spinal cord dorsal horn and dorsal root ganglion neurons of rats. Co-immunoprecipitation (Co-IP) and fluorescence lifetime-imaging-microscopy-based fluorescence resonance energy transfer (FLIM-FRET) assays showed that MOR heteromerized with CCKBR directly in transfected HEK293 cells. Combined with MOR mutant construction, the third transmembrane domain of MOR (TM3MOR) was demonstrated to participate in heteromerization with CCKBR. Receptor ligand binding, ERK phosphorylation and cAMP assays showed that MOR heteromerization with CCKBR weakened the activity of MOR. A cell-penetrating interfering peptide consisting of TM3MOR and TAT (a transactivator of HIV-1) sequences from the N terminal to the C terminal disrupted the MOR–CCKBR interaction and restored the activity of MOR in transfected HEK293 cells. Furthermore, intrathecal application of the TM3MOR-TAT peptide alleviated CCK-8-injection-induced antagonism to morphine analgesia in rats. These results suggest a new molecular mechanism for CCK-8 antagonism to opioid analgesia in terms of G-protein-coupled receptor (GPCR) interaction through direct heteromerization. Our study may provide a potential strategy for pain management with opioid analgesics.

IMPACTS OF EL NINO AND LA NINA ON FLOW DISCHARGE OF THE YANGTZE RIVER

HE YI-PING,WANG ZHAO-YIN,XIE XIAO-PING 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Effect of B-complex vitamins on the antifatigue activity and bioavailability of ginsenoside Re after oral administration

Yin Bin Chen,Yu Fang Wang,Wei Hou,Ying-Ping Wang,Sheng-Yuan Xiao,Yang Yang Fu,Jia Wang,Si Wen Zheng,Pei-He Zheng 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.2

Background: Both ginsenoside Re and B-complex vitamins are widely used as nutritional supplements. They are often taken together so as to fully utilize their antifatigue and refreshing effects, respectively. Whether actually a drugenutrient interaction exists between ginsenoside Re and B-complex vitamins is still unknown. The objective of this study was to simultaneously investigate the effect of B-complex vitamins on the antifatigue activity and bioavailability of ginsenoside Re after their oral administration. The study results will provide valuable theoretical guidance for the combined utilization of ginseng and B-complex vitamins. Methods: Ginsenoside Re with or without B-complex vitamins was orally administered to mice to evaluate its antifatigue effects and to rats to evaluate its bioavailability. The antifatigue activity was evaluated by the weight-loaded swimming test and biochemical parameters, including hepatic glycogen, plasma urea nitrogen, and blood lactic acid. The concentration of ginsenoside Re in plasma was determined by liquid chromatographyetandem mass spectrometry. Results: No antifatigue effect of ginsenoside Re was noted when ginsenoside Re in combination with Bcomplex vitamins was orally administered to mice. B-complex vitamins caused to a reduction in the bioavailability of ginsenoside Re with the area under the concentrationetime curve from zero to infinity markedly decreasing from 11,830.85 2,366.47 h$ng/mL to 890.55 372.94 h$ng/mL. Conclusion: The results suggested that there were pharmacokinetic and pharmacodynamic drugenutrient interactions between ginsenoside Re and B-complex vitamins. B-complex vitamins can significantly weaken the antifatigue effect and decrease the bioavailability of ginsenoside Re when simultaneously administered orally.

TP63 Gene Polymorphisms, Cooking Oil Fume Exposure and Risk of Lung Adenocarcinoma in Chinese Non-smoking Females

Yin, Zhi-Hua,Cui, Zhi-Gang,Ren, Yang-Wu,Su, Meng,Ma, Rui,He, Qin-Cheng,Zhou, Bao-Sen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: Genetic polymorphisms of TP63 have been suggested to influence susceptibility to lung adenocarcinoma development in East Asian populations. This study aimed to investigate the relationship between common polymorphisms in the TP63 gene and the risk of lung adenocarcinoma, as well as interactions of the polymorphisms with environmental risk factors in Chinese non-smoking females. Methods: A case-control study of 260 cases and 318 controls was conducted. Data concerning demographic and risk factors were obtained for each subject. The genetic polymorphisms were determined by Taqman real-time PCR and statistical analyses were performed using SPSS software. Results: For 10937405, carriers of the CT genotype or at least one T allele (CT/TT) had lower risks of lung adenocarcinoma compared with the homozygous wild CC genotype in Chinese nonsmoking females (adjusted ORs were 0.68 and 0.69, 95%CIs were 0.48-0.97 and 0.50-0.97, P values were 0.033 and 0.030, respectively). Allele comparison showed that the T allele of rs10937405 was associated with a decreased risk of lung adenocarcinoma with an OR of 0.78 (95%CI=0.60-1.01, P=0.059). Our results showed that exposure to cooking oil fumes was associated with increased risk of lung adenocarcinoma in Chinese nonsmoking females (adjusted OR=1.58, 95%CI=1.11-2.25, P=0.011). However, we did not observe a significant interaction of cooking oil fumes and TP63 polymorphisms. Conclusion: TP63 polymorphism might be a genetic susceptibility factor for lung adenocarcinoma in Chinese non-smoking females, but no significant interaction was found with cooking oil fume exposure.

The effects of blastocyst morphological score and blastocoele re-expansion speed after warming on pregnancy outcomes

Yin, Huiqun,Jiang, Hong,He, Ruibing,Wang, Cunli,Zhu, Jie,Li, Yang The Korean Society for Reproductive Medicine 2016 Clinical and Experimental Reproductive Medicine Vol.43 No.1

Objective: The aim of this study was to investigate associations between the morphology score of blastocysts and blastocoele re-expansion speed after warming with clinical outcomes, which could assist in making correct and cost-effective decisions regarding the appropriate time to vitrify blastocysts and to transfer vitrified-warmed blastocysts. Methods: A total of 327 vitrified-warmed two-blastocyst transfer cycles in women 38 years old and younger were included in this retrospective study. Results: The clinical pregnancy rate (CPR) and implantation rate (IR) of transfers of two good-morphology grade 4 blastocysts vitrified on day 5 (64.1% and 46.8%, respectively) were significantly higher than the CPR and IR associated with the transfers of two good-morphology grade 3 blastocysts vitrified on day 5 (46.7% and 32.2%, respectively). No significant differences were found in the CPR and IR among the transfers of two good-morphology grade 4 blastocysts regardless of the day of cryopreservation. Logistic regression analysis showed that blastocoele reexpansion speed after warming was associated with the CPR. Conclusion: The selection of a good-morphology grade 4 blastocyst to be vitrified could be superior to the choice of a grade 3 blastocyst. Extending the culture of grade 3 blastocysts and freezing grade 4 or higher blastocysts on day 6 could lead to a greater likelihood of pregnancy. Since re-expansion was shown to be a morphological marker of superior blastocyst viability, blastocysts that quickly re-expand after warming should be prioritized for transfer.