Circ_0081143 Contributes to Gastric Cancer Malignant Development and Doxorubicin Resistance by Elevating the Expression of YES1 by Targeting mziR-129-2-3p

( Wenting Ou ),( Lin Lin ),( Rihong Chen ),( Qingwen Xu ),( Caijin Zhou ) 대한소화기기능성질환·운동학회 2022 Gut and Liver Vol.16 No.6

Background/Aims: The increased mortality of gastric cancer (GC) is mainly attributed to the development of chemoresistance. Circular RNAs, as the novel type of biomarkers in GC, have attracted wide attention. The purpose of this study was to investigate the functional role of circ_0081143 in GC with doxorubicin (DR) resistance and its potential action mechanism. Methods: The expression of circ_0081143, miR-129-2-3p and YES proto-oncogene 1 (YES1) in GC tissues and cells was measured by quantitative real-time polymerase chain reaction. The half maximal inhibitory concentration value was calculated based on the MTT cell viability assay. Cell proliferation and apoptosis were monitored by MTT and flow cytometry assays. Transwell assays were employed to check cell migration and invasion. The protein levels of YES1 and apoptosis-related proteins were detected by western blotting. The interaction between miR-129-2-3p and circ_0081143 or YES1 was verified by dual-luciferase reporter and pull-down assays. A tumorigenicity assay was conducted to verify the role of circ_0081143 in vivo. Results: Circ_0081143 was highly expressed in DR-resistant GC tumor tissues and cells. Depletion of circ_0081143 reduced DR resistance and inhibited DR-resistant GC cell proliferation, migration and invasion. Circ_0081143 targeted miR-129-2-3p and inhibited the role of miR-129-2-3p. In addition, YES1 was a target of miR-129-2-3p, and its function was suppressed by miR-129-2-3p. Importantly, circ_0081143 positively modulated the expression of YES1 through mediating miR-129-2-3p. Circ_0081143 knockdown weakened the DR-resistant GC tumor growth in vivo. Conclusions: Circ_0081143 knockdown weakened DR resistance and blocked the development of DR-resistant GC by regulating the miR-129-2-3p/YES1 axis. Our data suggest that circ_0081143 is a promising target for the treatment of GC with DR resistance. (Gut Liver 2022;16:861-874)

Effect of Biaxial Loading Path on Seismic Performance of RC Bridge Piers with Corrosion Damage

Wenting Yuan,Yuren Wang,Peng Zhou,Wei Yuan,Yadong Yao 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.12

In recent years, the deterioration of seismic behavior for reinforced concrete (RC) bridges due to reinforcement corrosion has received increasing attention. Many studies have been performed to explore the seismic capacity of corroded piers by applying uniaxial cyclic loading. However, the uniaxial seismic responses of corroded piers cannot accurately reflect the true structural response influenced by corrosion condition and real multidimensional earthquake action. Pointing at this problem, in this study, the seismic performance of corroded pier columns suffering biaxial lateral cyclic loadings is investigated by conducting comprehensive numerical analyses. First, a fiber-based numerical model is built and verified by the cyclic experiment results of two RC pier columns under different displacement loading patterns and corrosion levels. Then, three corrosion levels and five uniaxial and biaxial displacement protocols are used in the verified numerical models. The simulation results show that the ultimate force, deformation capacity, and dissipated energy of the piers are significantly influenced by corrosion degrees and biaxial loading patterns compared with the uniaxially loaded uncorroded piers. The phase lag between the biaxial displacement trajectory and biaxial force trajectory has three stages: a small-range fluctuation stage, a wide-range fluctuating and rise stage, and a wide-range fluctuating and decrease stage. The RE pattern is the most detrimental pattern, which leads to the fastest damage accumulation of the same-level corroded bridge piers.

Minor and major circRNAs in virus and host genomes

Lou Zhihao,Zhou Rui,Su Yinghua,Liu Chun,Wenting Ruan,전체옥,Xiao Han,Chun Lin,BAOLEI JIA 한국미생물학회 2021 The journal of microbiology Vol.59 No.3

As a special type of noncoding RNA, circular RNAs (circRNAs) are prevalent in many organisms. They can serve as sponges for microRNAs and protein scaffolds, or templates for protein translation, making them linked to cellular homeostasis and disease progression. In recent years, circRNAs have been found to be abnormally expressed during the processes of viral infection and pathogenesis, and can help a virus escape the immune response of a host. Thus, they are now considered to play important functions in the invasion and development of viruses. Moreover, the potential application of circRNAs as biomarkers of viral infection or candidates for therapeutic targeting deserves consideration. This review summarizes circRNAs in the transcriptome, including their classification, production, functions, and value as biomarkers. This review paper also describes research progress on circRNAs in viral infection (mainly hepatitis B virus, HIV, and some human herpes viruses) and aims to provide new ideas for antiviral therapies targeting circRNAs.

Cerium-loaded MnOx/attapulgite catalyst for the low-temperature NH3-selective catalytic reduction

Aijuan Xie,Xingmeng Zhou,Xiaoyan Huang,Liang Ji,Wenting Zhou,Shiping Luo,Chao Yao 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.49 No.-

A series of MnO2/attapulgite (ATP) and n(Ce):n(Mn)/ATP (molar ratios) catalysts were prepared andinvestigated for the selective catalytic reduction of NO by NH3 (NH3-SCR) at low temperature. The resultsshowed that the 7 wt % MnO2/ATP exhibited the best NOx conversion (85% at 300 C) among all MnO2/ATPcatalysts of different mass ratios. The introduction of cerium enhanced the NOx conversion at lowtemperature, and so Ce–MnOx/ATP can reach the highest NOx conversion (95% at 300 C). Meanwhile, theas-prepared catalysts were characterized by XRD, TEM, BET, H2-TPR, NH3-TPD, and XPS. It can be deducedfrom TEM, XRD, and BET, MnOx nanorods in this work mainly existed in the b-MnO2, and cerium highlydispersed on the surface of ATP to form porous structure and thus improved the deNOx performance. Moreover, the study of SO2 tolerance demonstrated that cerium can effectively inhibit SO2 poison. XPSresults illustrated that Ce could enhance Mn4+ content on the surface of the catalyst and thus lead to highSCR activity. Therefore Mn(1):Ce(0.25)/ATP was proved to be an excellent catalyst for NH3-SCR.

Interaction between rice bran albumin and epigallocatechin gallate and their physicochemical analysis

Rui Yang,Yuqian Liu,Jingjing Xu,Wenting Shang,Xiaoyuan Zhang,Yongjin Wang,Chris Blanchard,Zhongkai Zhou 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.6

Epigallocatechin gallate (EGCG) is sensitive to heat thus its application in food industry is limited. In this work, rice bran albumin protein (RAP) was used as a carrier for EGCG. RAP-EGCG complexes (RAPE) were prepared with the binding number n of 0.0505:1 (EGCG: RAP, w/w) and binding constant K of (0.74 ± 0.002) 9 104 M-1, which suggests that hydrogen bond/van der Waals forces played important roles in such binding. FTIR analysis demonstrated that EGCG could induce the secondary structure changes of RAP above the ratio of 1.92:1 (EGCG:RAP, w/w). Dynamic light scattering and scanning electron microscope results showed that EGCG could trigger RAP association. Furthermore, the EGCG stability in RAPE was significantly improved than that of free EGCG in 10–60 C. The antioxidant ability of EGCG in RAPE was partially retained. These findings prove that RAP is a potential carrier for polyphenols and is beneficial for mechanism investigation between protein and polyphenols.

Experimental study on shear, tensile, and compression behaviors of composite insulated concrete sandwich wall

Zhang, Xiaomeng,Zhang, Xueyong,Liu, Wenting,Li, Zheng,Zhang, Xiaowei,Zhou, Yilun Techno-Press 2021 Advances in concrete construction Vol.11 No.1

A new type of composite insulated concrete sandwich wall (ICS-wall), which is composed of a triangle truss steel wire network, an insulating layer, and internal and external concrete layers, is proposed. To study the mechanical properties of this new ICS-wall, tensile, compression, and shearing tests were performed on 22 specimens and tensile strength and corrosion resistance tests on 6 triangle truss joints. The variables in these tests mainly include the insulating plate material, the thickness of the insulating plate, the vertical distance of the triangle truss framework, the triangle truss layout, and the connecting mode between the triangle truss and wall and the material of the triangle truss. Moreover, the failure mode, mechanical properties, and bearing capacity of the wall under tensile, shearing, and compression conditions were analyzed. Research results demonstrate that the concrete and insulating layer of the ICS-wall are pulling out, which is the main failure mode under tensile conditions. The ICS-wall, which uses a graphite polystyrene plate as the insulating layer, shows better tensile properties than the wall with an ordinary polystyrene plate. The tensile strength and bearing capacity of the wall can be improved effectively by strengthening the triangle truss connection and shortening the vertical distances of the triangle truss. The compression capacity of the wall is mainly determined by the compression capacity of concrete, and the bonding strength between the wall and the insulating plate is the main influencing factor of the shearing capacity of the wall. According to the tensile strength and corrosion resistance tests of Austenitic stainless steel, the bearing capacity of the triangle truss does not decrease after corrosion, indicating good corrosion resistance.