Temporal and Spatial Change in Microbial Diversity in New-developed Wetland Soil Covered by Tamarix chinesis Community in Chinese Yellow River Delta

Chen, WeiFeng,Ann, Seoung-Won,Kim, Hong-Nam,Shi, Yanxi,Mi, Qinghua 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4

Soil samples were collected from new-developed wetland soil ecosystem of Tamarix chinesis plantation in Chinese Yellow River Delta in different months of 2003. Soil characteristics, temporal change and spatial distribution of microbial community composition and their relationship with nitrogen turnover and circling were investigated in order to analyze and characterize the role of microbial diversity and functioning in the specific soil ecosystem. The result showed that the total population of microbial community in the studied soil was considerably low, compared with common natural ecosystem. The amount of microorganism followed as the order: bacteria> actinomycetes>fungi. Amount of actinomycetes were higher by far than that of fungi. Microbial population remarkably varied in different months. Microbial population of three species in top horizon was corrected to that in deep horizon. Obvious rhizosphere effect was observed and microbial population was significantly higher in rhizosphere than other soils due to vegetation growth, root exudation, and cumulative dead fine roots. Our results demonstrate that microbial diversity is low, while is dominated by specific community in the wetland ecosystem of Tamarix chinesi.

Distribution Characteristic and Assessment of Soil Organic Matter, Nitrogen and Phosphorus in Soils of New born River Mouth Wetlands

Chen, Weifeng,Ann, Seoung-Won,Shi, Yanxi,Mi, QingHua 한국환경과학회 2003 한국환경과학회지 Vol.12 No.2

The paper investigates preliminarily spatial distribution soil organic matter (SOM), nitrogen (N) and phosphorus (O) and its environmental influence in wetland soil of different vegetation landscape in the Yellow River Mouth. The result shows the SOM and total nitrogen (TN), efficient N, efficient P in top later soils of different vegetation district have signficantly different content, The SOM is show as Calamagrostis epigeios wetlands >Phragmites cmmunis wetlands >Tamarix chinensi wetlands above tidal >Suaeda salsa wetlands in high tidal >Tamarix chinensi wetlands in high tidal >tidal flats, the arrange of the TN and efficient N content is the same except that the content in Suaeda salsa wetlands in high tidal is heavier than Tamarix chinensi wetlands in high tidal. In different vegetation landscape wetland types the vertical change of soil nutrients are obvious except for P, gradually decrease from the upper to the lower. This case reflects the function of the vegetation on the wetland development of soil, and proves the wetland soil has the characteristic of new born and bad degree of development, SOM, TM, efficient N and efficient P content in wetland soils have significantly positive correlation, but TP have no correlation with them but efficient P. The contents of TN in wetland soils range from 58∼1480㎎/㎏, total average content 408 ㎎/㎏, average content of above 30 cm is 625 ㎎/㎏. The range of TP content in the soil is 372∼1042㎎/㎏, total average is 569 ㎎/㎏, average content of above 20 cm is 611㎎/㎏. According the P it occurs mainly as calcium phosphates, and the validity is lower, therefore, N and P in the new born wetlands cannot produce serious impact on the environments at present.

Influence of the River Ceasing on Wetland Environment in the Yellow River Delta

Chen, Weifeng,Shi, Yanxi,Mi, Qinghua,Ann, Seoung-Won 한국환경과학회 2003 한국환경과학회지 Vol.12 No.2

The yellow River delta is an important region where coastal and newly formed wetlands distribute in north Chine. Based on satellite remote sensing images and GIS thchniques, this paper tends to delineate the dynamic changes of newly formed wetland in the Yellow River mouth from 1986.5 To 1996.10. Our results show that the newly formed wetland increased by 24.9 ㎢ per year. Before 1990.1 and it decreased by 2.40 ㎢ per year after that. The northern and southwestern parts of the Yellow River mouth are main positions of decrease and the southern and the estuary parts are main positions of increase. The advancing rate of river mouth extending into the Bo Sea is decreasing obviously. The reason for that is the decreasing of water and sediments in the Yellow River, Which caused by the increasing use of water and soil conservation on upper reath.

HPF1 regulates tendon stem/progenitor cell senescence and tendon repair via PARP1-mediated poly-ADP ribosylation of HuR

Han Weifeng,GU Dongqiang,Chen Hongguang,Tao Xu,Chen Lei 한국유전학회 2024 Genes & Genomics Vol.46 No.1

Background Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair, regeneration and homeostasis. However, the specific mechanism of TSPCs aging is still unclear. Objective This study aims to explore the role and molecular mechanism of HPF1 in the aging of TSPCs. Methods Young and aged TSPCs (Y-TSPCs and A-TSPCs) were acquired from 3 to 4 and 24–26-month-old Sprague–Dawley male rats, TSPCs (Y-TSPCs and A-TSPCs) were subjected to senescence-associated β-galactosidase (SA-β-Gal))staining and telomerase activity detection, p16, p21, Scx, Tnmd, Col1, Col3HPF1 and PAPR1 expression levels were detected by Western blot or Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR), Reciprocal co-immunoprecipitation (co-IP) was used to explore the interaction between HPF1 and PARP1. Ribonucleoprotein immunoprecipitation (RNP-IP) was used to analyze the binding of HuR to the senescence marker gene mRNAs, IP was used to perform HPF1 to the PARylation of HuR, and the half-life of p16 and p21 were detected. Finally, we established an in vivo model, and the tendon tissue was used to perform hematoxylin and eosin (HE) and masson’s trichrome staining, as well as the immunohistochemical analysis of Col I and TNMD. Results Compared with Y-TSPCs, A-TSPCs had significantly enhanced cell senescence and significantly reduced tendon differentiation ability, and significantly increased the expression of HPF1 and PARP1. In addition, HPF1 and PARP1 interacted and coordinated the senescence and differentiation of TSPCs, HPF1 could also regulate the expression of p21 and p21, the interaction of p16 or p21 with HuR, and the poly-ADP ribosylation of PARP1 to HuR. HPF1 overexpression and siHuR co-transfection significantly reduced the half-life of p16 and p21, and HPF1 and PARP1 regulated the mRNA levels of p16 and p21 through HuR. Finally, in vivo experiments have shown that HPF1 or PARP1 overexpression could both inhibit the ability of tendon differentiation and promote cell senescence. Conclusions HPF1 promoted the senescence of TSPCs and inhibits the tendon differentiation of TSPCs through PARP1-mediated poly-ADP ribosylation of HuR. Similar content being viewed by others Background Tendon stem/progenitor cells (TSPCs) play a vital role in tendon repair, regeneration and homeostasis. However, the specific mechanism of TSPCs aging is still unclear. Objective This study aims to explore the role and molecular mechanism of HPF1 in the aging of TSPCs. Methods Young and aged TSPCs (Y-TSPCs and A-TSPCs) were acquired from 3 to 4 and 24–26-month-old Sprague–Dawley male rats, TSPCs (Y-TSPCs and A-TSPCs) were subjected to senescence-associated β-galactosidase (SA-β-Gal))staining and telomerase activity detection, p16, p21, Scx, Tnmd, Col1, Col3HPF1 and PAPR1 expression levels were detected by Western blot or Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR), Reciprocal co-immunoprecipitation (co-IP) was used to explore the interaction between HPF1 and PARP1. Ribonucleoprotein immunoprecipitation (RNP-IP) was used to analyze the binding of HuR to the senescence marker gene mRNAs, IP was used to perform HPF1 to the PARylation of HuR, and the half-life of p16 and p21 were detected. Finally, we established an in vivo model, and the tendon tissue was used to perform hematoxylin and eosin (HE) and masson’s trichrome staining, as well as the immunohistochemical analysis of Col I and TNMD. Results Compared with Y-TSPCs, A-TSPCs had significantly enhanced cell senescence and significantly reduced tendon differentiation ability, and significantly increased the expression of HPF1 and PARP1. In addition, HPF1 and PARP1 interacted and coordinated the senescence and differentiation of TSPCs, HPF1 could also regulate the expression of p21 and p21, the interaction of p16 or p21 with HuR, and the poly-ADP ribosylation of PARP1 to HuR. HPF1 overexpression and siHuR co-transfection significantly reduced the half-life of p16 and p21, and HPF1 and PARP1 regulated the mRNA levels of p16 and p21 through HuR. Finally, in vivo experiments have shown that HPF1 or PARP1 overexpression could both inhibit the ability of tendon differentiation and promote cell senescence. Conclusions HPF1 promoted the senescence of TSPCs and inhibits the tendon differentiation of TSPCs through PARP1-mediated poly-ADP ribosylation of HuR. Similar content being viewed by others

Identification and confirmation of 14-3-3 ζ as a novel target of ginsenosides in brain tissues

Feiyan Chen,Lin Chen,Weifeng Liang,Zhengguang Zhang,Jiao Li,Wan Zheng,Zhu Zhu,Jiapeng Zhu,Yunan Zhao 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.4

Background: Ginseng can help regulate brain excitability, promote learning and memory, and resist cerebral ischemia in the central nervous system. Ginsenosides are the major effective compounds of Ginseng, but their protein targets in the brain have not been determined. Methods: We screened proteins that interact with the main components of ginseng (ginsenosides) by affinity chromatography and identified the 14-3-3 ζ protein as a potential target of ginsenosides in brain tissues. Results: Biolayer interferometry (BLI) analysis showed that 20(S)-protopanaxadiol (PPD), a ginseng saponin metabolite, exhibited the highest direct interaction to the 14-3-3 ζ protein. Subsequently, BLI kinetics analysis and isothermal titration calorimetry (ITC) assay showed that PPD specifically bound to the 14-3-3 ζ protein. The cocrystal structure of the 14-3-3 ζ protein-PPD complex showed that the main interactions occurred between the residues R56, R127, and Y128 of the 14-3-3 ζ protein and a portion of PPD. Moreover, mutating any of the above residues resulted in a significant decrease of affinity between PPD and the 14-3-3 ζ protein. Conclusion: Our results indicate the 14-3-3 ζ protein is the target of PPD, a ginsenoside metabolite. Crystallographic and mutagenesis studies suggest a direct interaction between PPD and the 14-3-3 ζ protein. This finding can help in the development of small-molecular compounds that bind to the 14-3-3 ζ protein on the basis of the structure of dammarane-type triterpenoid.

Research and Application of Fiber Bragg Grating Sensor in Geological Disaster Automation Monitoring

Liu Yimin,Wang Jie,Ji Weifeng,Zhou Ce,Chen Wenjun 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10

In this paper, fiber grating technology is used for accurately measure stresses, temperature and displacement. The paper introduces the basic principles of FBG technology and the wavelength demodulation method of FBG sensors, and put fiber grating technology into practical engineering projects. The paper design a optical fiber grating real-time automation monitoring system to distributed measure stress, temperature and displacement in geological disaster body, to provide effective technical supports for geological disaster prevention and mitigation.

Resilient Filtering for Delayed Markov Jump Neural Networks via Event-triggered Strategy

Weifeng Xia,Yongmin Li,Zuxin Li,Shuxin Du,Bo Li,Wenbin Chen 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.10

This paper deals with the event triggered filtering problem for a class of delayed discrete-time Markov jump neural networks, where a resilient filter with parameter uncertainties is adopted. The aim of this paper is to design a suitable filter which ensures that the filtering error system is stochastically stable and satisfies a prescribed mixed passivity and H∞ performance. Sufficient conditions for solvability of such a problem are developed. Basedon the obtained conditions, an explicit expression of the desired resilient filter is proposed. Finally, an example is presented to show the usefulness of the proposed scheme.

Collaborative Optimization Design of Event-based Asynchronous Filter for Discrete-time T-S Fuzzy Markov Jump Systems

Huiying Chen,Weifeng Xia,Zheng-Guang Wu,Zuxin Li 제어·로봇·시스템학회 2024 International Journal of Control, Automation, and Vol.22 No.3

This article is primarily concerned with the collaborative optimization design issue of the asynchronousfilter and the event-based trigger for discrete-time nonlinear Markov jump systems modeled by the Takagi-Sugenofuzzy model. In the network environment, an event-based trigger is introduced to reduce communication consumption while the hidden Markov model approach is utilized to express the asynchronous phenomenon between thefilter and the plant. Based on this framework, using the parameter-dependent Lyapunov function and matrix inequality techniques, the H∞ and L2 −L∞ asynchronous filter design schemes are developed such that the resultingfiltering error dynamic system can be randomly mean square stable and satisfy a preset noise attenuation performance. Furthermore, an iterative optimization algorithm is presented to collaboratively solve the optimal parametersof the filter and the trigger. At last, a tunnel-diode circuit is employed to verify the validity of our design schemes.

Pharmacokinetics, tissue distribution and excretion of peimisine in rats assessed by liquid chromatography-tandem mass spectrometry

Lihua Chen,Dongxun Li,Guosong Zhang,Wei Zhang,Lihua Zhang,Yongmei Guan,Weifeng Zhu,Hongning Liu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Peimisine, the common ingredient of ‘‘zhebeimu’’groups and ‘‘chuanbeimu’’ groups, is responsiblefor the expectorant and cough relieving effects. The aim ofthis study was to investigate the pharmacokinetics, tissuedistribution and excretion of peimisine in male and femaleSD (Sprague-Dawley) rats by a rapid and sensitive LC-MS/MS (liquid chromatography-tandem mass spectrometry)method used carbamazepine as the internal standard afteroral administration, carbamazepine was stated as an IS. The results showed that peimisine was slowly distributed,and eliminated from rat plasma and manifested lineardynamics in a dose range of 0.26–6.5 mg/kg. Tested byANOVA, there were gender differences in the pharmacokineticparameters of AUC0-t, AUC0-? among a singledose of 0.26, 1.3, 6.5 mg/kg (P\0.05). Drug blood andtissue levels in male rats were significantly higher than thefemale counterparts after oral administration, while boththe males and the females showed high drug levels inspleen, kidney, lung, liver and heart. On the other hand, thepeimisine levels that can be reached in uterus, ovary, testisand brain is low. The excretion study showed that littleadministered peimisine (\0.7 %) was recovered in themale and female bile. Approximately 13.46 and 15.05 %were recovered in female urine and feces, while 43.07 and7.49 % were recovered in male urine and feces, respectively,which indicated that the major elimination route ofmale rats was urine excretion. In addition, there was significantdifferences in total cumulative excretive ratio ofpeimisine in feces (P\0.05) and no significant differencesin the urine (P[0.05) at a dose of 1.3 mg/kg.

Cauchy mutation boosted Harris hawk algorithm: optimal performance design and engineering applications

Shan Weifeng,He Xinxin,Liu Haijun,Heidari Ali Asghar,Wang Maofa,Cai Zhennao,Chen Huiling 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.2

Harris hawks optimization (HHO) has been accepted as one of the well-established swarm-based methods in the community of optimization and machine learning that primarily works based on multiple dynamic features and various exploratory and exploitative traits. Compared with other optimization algorithms, it has been observed that HHO can obtain high-quality solutions for continuous and constrained complex and real-world problems. While there is a wide variety of strategies in the HHO for dealing with diverse situations, there are chances for sluggish performance, where the convergence rate can gradually slow with time, and the HHO may stay stuck in the current relatively better place and may be unable to explore other better areas. To mitigate this concern, this paper combines the Cauchy mutation mechanism into the HHO algorithm named CMHHO. This idea can boost performance and provide a promising optimizer for solving complex optimization problems. The Cauchy mutation mechanism can speed up the convergence of the solution and help HHO explore more promising regions compared to its basic release. On 30 IEEE CEC2017 benchmark functions, the study compared the proposed CMHHO with various conventional and advanced metaheuristics to validate its performance and quality of solutions. It has been found through experiments that the overall optimization performance of CMHHO is far superior to all competitors. The CMHHO method is applied to four engineering challenges to investigate the capabilities of the proposed algorithm in solving real-world problems, and experimental results show that the suggested algorithm is more successful than existing algorithms.