MiR-200c-3p aggravates gastric cell carcinoma via KLF6

Wang Ying,Lu Kaijuan,Li Weibing,Wang Zhigang,Ding Jing,Zhu Zeyu,Li Zhipeng 한국유전학회 2021 Genes & Genomics Vol.43 No.11

Background Gastric cell carcinoma (GCC) is a common and high-incidence malignant gastrointestinal cancer that seriously threatens human life and safety. Evidences suggest that microRNAs (miRNAs) exhibit an essential role in regulating the occurrence and development of GCC, while the efects and possible mechanisms remain to be further explored. Objective This study was designed to explore whether miR-200c-3p exerted its functional role in the growth and metastasis of GCC, and investigate the possible mechanisms. Methods The expression levels of miR-200c-3p in GCC tissues and cell lines were detected by qRT-PCR analysis. The functional role of miR-200c-3p in the viability, proliferation, migration and invasion of GCC cells were evaluated by CCK-8, EdU, wound healing and Transwell assays. In addition, the candidate targets of miR-200c-3p was predicted and confrmed by dual-luciferase reporter assay. Moreover, the relationship between miR-200c-3p and target (Krüppel like factor 6, KLF6) was assessed by qRT-PCR and western blot assays. Besides, the expression levels of KLF6 in GCC cells were determined by qRT-PCR and western blot assays. Furthermore, the role of KLF6 in the viability, proliferation, migration and invasion of GCC cells mediated with miR-200c-3p mimics was evaluated by CCK-8, EdU, wound healing and Transwell assays. Results In the present study, a new tumor promoting function of miR-200c-3p was disclosed in GCC. We found that the expression of miR-200c-3p was obviously increased in clinic GCC tissues and cell lines. In addition, down-regulation of miR-200c-3p suppressed cell viability, proliferation, migration, and invasion in GCC cells. Moreover, KLF6 was verifed as a direct target of miR-200c-3p by binding its 3’-UTR. Additionally, KLF6 was remarkably decreased and was negatively associated with the miR-200c-3p expression in GCC cell lines. Furthermore, over-expression of KLF6 retarded the efects of miR-200c-3p on the growth and metastasis of GCC cell lines. Conclusions MiR-200c-3p potentially played a tumor-promoting role in the occurrence and development of GCC, which may be achieved by targeting KLF6.

Study on the Kinetic Characteristics of Microbubbles in Cross-Shaped Flow Focusing Microchannels

Ding Weibing,Yang Qianwen,Zhao Yaohui,Wang Zhaohui,Chen Jie,Wang Hongxia 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.1

To study the mechanism of microbubbles generation in cross-shaped microchannels, numerical simulations of gas–liquid two-phase fl ow in microchannels are carried out in this paper using the volume of fl uid method (VOF). By varying the twophase fl ow rate, three diff erent fl ow regimes were obtained, including dripping regime, slugging regime and threading regime, and the relationship between the two-phase fl ow rate and the fl ow state was plotted. Meanwhile, the phase interface, pressure and velocity of microbubbles in three diff erent fl ow regimes were studied, and the evolution of the gas–liquid interface in microbubbles formation was analyzed. It is found that the microbubbles diameter decreases and the frequency increases as the viscosity of the continuous phase gradually increases. As the wall contact angle decreases, the adhesion of the liquid phase to the wall at the channel interaction increases and the microbubbles diameter increases. The increase in interfacial tension greatly increases the cohesion between molecules on the surface of the gas fl ow, making it diffi cult to achieve force equilibrium, which leads to a reduction in the shear stress required to dominate the interface to break the tip of the gas fl ow and slower bubbles formation, resulting in a larger microbubbles diameter.

Long-term organic-inorganic fertilization ensures great soil productivity and bacterial diversity after natural-to-agricultural ecosystem conversion

Weibing Xun,Zhihui Xu,Wei Li,Yi Ren,Ting Huang,Wei Ran,Boren Wang,Qirong Shen,Ruifu Zhang 한국미생물학회 2016 The journal of microbiology Vol.54 No.9

Natural ecosystems comprise the planet’s wild plant and animal resources, but large tracts of land have been converted to agroecosystems to support the demand for agricultural products. This conversion limits the number of plant species and decreases the soil biological diversity. Here we used highthroughput 16S rRNA gene sequencing to evaluate the responses of soil bacterial communities in long-term converted and fertilized red soils (a type of Ferralic Cambisol). We observed that soil bacterial diversity was strongly affected by different types of fertilization management. Oligotrophic bacterial taxa demonstrated large relative abundances in chemically fertilized soil, whereas copiotrophic bacterial taxa were found in large relative abundances in organically fertilized and fallow management soils. Only organic-inorganic fertilization exhibited the same local taxonomic and phylogenetic diversity as that of a natural ecosystem. However, the independent use of organic or inorganic fertilizer reduced local taxonomic and phylogenetic diversity and caused biotic homogenization. This study demonstrated that the homogenization of bacterial communities caused by natural-to-agricultural ecosystem conversion can be mitigated by employing rational organic-inorganic fertilization management.

Research on the cone angle and clearance of main pump seal

Wang Heshun,Zhu Weibing,Huang Zepei,Zhang Chening,Zhang Junkai 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.7

For better film characteristics of the main pump seal (MPS), a theoretical calculation model was proposed based on the Reynolds equation. A case study with defined MPS was carried out, film stiffness was taken as the basic constraint condition, and the cone angle andclearance are two optimization targets. When the film stiffness reaches a maximum, the corresponding cone angle and clearance is theoptimal value. The results show that: (1) film stiffness increased and decreased with clearance and cone angle, respectively; (2) the optionalclearance was determined to be within a range of 2~8 μm at a cone angle of between 0.1~1.2'; (3) the maximum of film stiffness isinversely proportional to the cone angle (Clearance) approximately under the clearance (Cone angle) is fixed; (4) leakage is directly proportionalto the clearance to power three, and the clearance has greater influence on the amount of leakage than the cone angle. Theseresults provide a reliable theoretical justification for MPS-based designs including optimization method. It therefore forms a fundamentalbase for research and practical application.

Analysis of Cable under Dynamic Contact and Large Deformation

Bingjian Wang,Qingbin Li,Tianyun Liu,Weibing Peng 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

For simulating contact interactions and high displacement gradients between the cable and the saddle at the middle tower of tripletower suspension bridges, a cable element is developed by combining the absolute nodal coordinate formulation and the quasiconforming technique. New curvature strains are developed and elastic forces are explicitly formulated for the cable elements. Thereafter, it is compared to the original one to verify its locking remedies. The numerical solutions using the element are compared to analytical results and solutions by the original element. Compared to the original, the proposed element suppresses the highfrequency disturbances in the velocity and acceleration curves. Using the element, the contact and sliding behavior between the cable and the saddle is analyzed by employing parameters obtained experimentally. The saddle’s mechanical and frictional performance subjected to different friction coefficients and unbalanced cable forces is investigated. The proposed model exhibits excellent accuracy in the prediction of the sliding force and the contact status between the cable and the saddle.

Development countermeasures of China's renewable energy industry under the influence of financial crisis

Jinqiang Wang,Weibing Cao 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

The vigorous development and use of renewable energy is an important strategic choice to optimize the struction of energy resources, safeguard the security of energy resources, alleviate environment pollution and balance the development of energy, ecnomoy and environment. Based on the analysis of positive and negative impacts of financial crisis on China"s renewable energy industry. some deveolpment countermeasures of this industry under the influence of financial crisis are proposed.

Fluid Mud Dynamics and Its Correlation to Hydrodynamics in Jiaojiang River Estuary, China

Li Li,Wang Jiachen,Zheng Yiqun,Yao Yanming,Guan Weibing 한국해양과학기술원 2023 Ocean science journal Vol.58 No.1

The Jiaojiang Estuary is a macro-tidal estuary with high turbidity and funnel-shaped geomorphology. Estuarine geomorphology and shipping are highly afected by sediments and heavy coastal engineering. Based on observed data and numerical simulation results, we studied the characteristics of the suspended sediments and fuid mud in the estuary. By considering two-way coupling of water–sediment density and the process of fne sediment focculation, a three-dimensional sediment model of the Jiaojiang Estuary was established and efectively calibrated using the measured data on tides, currents, and suspended sediment concentration (SSC). Field data analysis indicated that SSC and sediment transport rate in both the main tidal channel and shoals were positively correlated with the fow velocity in the low-frequency part during both the spring and neap tides. The model results revealed that the net sediment fux is controlled by advection and moves landward upstream of the main tidal channel. Fluid mud is formed near Haimen station, with tides infuencing the spatial and temporal variations in its thickness and speed. Sediment is actively exchanged among fuid mud-water-seabed, with sink and source processes dominating near the bay mouth and the Haimen station, respectively. Sediments weaken the seaward residual currents slightly by changing their viscosity and the von Karman constant, and the stratifcation process is afected by changes in water density. The fndings of this study provide a foundation for the study of material transportation in an estuarine ecosystem.

Performance of Gas-Liquid Upstream Pumping Spiral Groove Mechanical Face Seals

Baoyu Zhang,Weibing Wang,Tiebo Sun,Ping Li 한국유체기계학회 2024 International journal of fluid machinery and syste Vol.17 No.1

Upstream pumping spiral grooves provides an effective way to realize design of mechanical seals with long service life as well as low leakage rate, which largely lies on precise design of spiral grooves under gas-liquid lubricating boundary conditions. The originality of this paper is to consider the gas phase pressure boundary in order to be suitable for more complex gas-liquid seal analysis. An numerical analysis of performance of upstream pumping spiral groove mechanical face seals is carried outunder gas-liquid boundary conditions. Opening force and leakage rate are calculated taking account of gas-liquid fluid medium boundary conditions as well as cavitation effect. The results show that the opening force and leakage rate decrease obviously under gas-liquid boundary conditions, compared with the full liquid boundary conditions, exceeding 50% in degree. Besides, the groove parameters such as spiral angle, slotting ratio and groove number present obvious and complex influence on the opening force and leakage rate. This research may provide the reasonable basis for the design of upstream pumping spiral groove mechanical face seals.

Design of vortex finder structure for decreasing the pressure drop of a cyclone separator

Yaquan Sun,Junzhi Yu,Weibing Wang,Shanglin Yang,Xue Hu,Jingan Feng 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5

The structure of the vortex finder has an important influence on the pressure drop and separation efficiency of a cyclone, which mainly governs the separation process. In this paper, the traditional vortex finder is slotted on side wall and its bottom is closed, i.e., a slotted vortex finder. The impact of slotted vortex finder on the separation performance of a cyclone is explored by using numerical simulation and experimental validation. Specifically, the gas phase is studied by the Reynolds stress model (RSM), and the particle phase is simulated by the discrete phase model (DPM). The simulation results are in good agreement with the experimental results, revealing higher prediction accuracy. The results indicate that the slotted vortex finder can effectively suppress the generation of the downward swirling flow at the center of the vortex finder and decrease the turbulence intensity at the bottom of the vortex finder and the outer vortex, thereby decreasing the energy loss and increasing the separation efficiency. When the slot length is 0.2De, the slotted vortex finder can reduce the pressure drop by 143.33 Pa while increasing the collection efficiency by 5.51%.

Effect of a Multi-stage Processing Method on the Composite Properties of a Stainless-Steel Surface

Jing Li,Jianan Cao,Lida Pan,Feng Du,Weibing Wang,Chengyu Xu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.4

In this study, a multi-stage sandblasting and acid-etching processing method is used to prepare a 304 stainless-steel surfacewith a loose structure that has the composite properties of superhydrophobicity, low adhesion, anti-icing ability, self-cleaningbehavior and corrosion resistance. The surface morphology and chemical constituents of this structure are characterizedby scanning electron microscopy and X-ray diffraction, respectively. The results show that the micro-scale roughness anddifferent degrees of porosity in this structure play a key role in its superhydrophobicity.The anti-icing properties and corrosionresistance are characterized by contact-angle measurements and electrochemical workstation analyses, respectively. The results show that superhydrophobicity has positive effects on surface self-cleaning, anti-icing properties and corrosionresistance of 304 stainless-steel.