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Bovine Viral Diarrhea Virus Infection Induces Autophagy in MDBK Cells
Qiang Fu,Huijun Shi,Yan Ren,Fei Guo,Wei Ni,Jun Qiao,Pengyan Wang,Hui Zhang,Chuangfu Chen 한국미생물학회 2014 The journal of microbiology Vol.52 No.7
Bovine viral diarrhea virus (BVDV) is an enveloped, positive-sense, single-stranded RNA virus that belongs to the genus Pestivirus (Flaviviridae). The signaling pathways and levels of signaling molecules are altered in Madin-Darby Bovine Kidney (MDBK) cells infected with BVDV. Autophagy is a conservative biological degradation pathway that mainly eliminates and degrades damaged or superfluous organelles and macromolecular complexes for intracellular recycling in eukaryotic cells. Autophagy can also be induced as an effective response to maintain cellular homeostasis in response to different stresses, such as nutrient or growth factor deprivation, hypoxia, reactive oxygen species exposure and pathogen infection. However, the effects of BVDV infection on autophagy inMDBK cells remain unclear. Therefore, we performed an analysis of autophagic activity after BVDV NADL infection using real-time PCR, electron microscopy, laser confocal microscopy, and Western blotting analysis. The results demonstrated that BVDV NADL infection increased autophagic activity and significantly elevated the expression levels of the autophagy-related genes Beclin1 and ATG14 inMDBK cells. However, the knockdown of Beclin1 and ATG14 by RNA interference (RNAi) did not affect BVDV NADL infection-related autophagic activity. These findings provided a novel perspective to elaborate the effects of viral infection on the host cells.
Fu Rong Cao,Guo Qiang Xue,Bi Jin Zhou,Shun Cheng Wang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3
To explore the ductility, a novel Mg–8.41Li–1.80Al–1.77Zn (designated as LAZ822) alloy was fabricated by casting,hot rolling and friction stir processing. The maximum elongation to failure of 489.5% was demonstrated in a fine-grainedLAZ822 alloy at a temperature of 573 K and an initial strain rate of 1.67 × 10−4 s−1. The true stress exponent of 2, the grainsize exponent of 2 and the activation energy of 89.44–121.14 kJ/mol confirm that grain boundary sliding controlled by latticediffusion governs the rate-controlling deformation process at the temperatures of 523 and 573 K. The viscous resistancemodels of dual phases were newly established. At 573 K, the lattice viscous resistance of the α-Mg phase is 2644 times aslarge as that of the β-Li phase, whereas the grain boundary viscous resistance of the α-Mg phase is 3.3 times as large as thatof the β-Li phase. Some α-Mg grains remain in an equiaxed state while the other α-Mg grains become connected at elevatedtemperatures. This experimental evidence corroborates the existence of dynamic grain connection growth. Cavity growthmechanism maps were constructed. The maps reveal that power-law cavity growth or strain controlled cavity growth is thepredominant cavity growth mechanism.
Research on the Relation between Transformer Oil Flow Electrification and Electrostatic Current
Qiang Fu,Rui Wang,Pinguo Zou,Zhao Li,Yang Yang,Xuejun Xie 대한전기학회 2015 Journal of Electrical Engineering & Technology Vol.10 No.2
In order to study and obtain the mathematical relation between the electrification degree of transformer oil flow and the electrostatic current, a small amount of data about the electrification degree of oil flow and the corresponding electrostatic current is studied by linear regression method and grey model method. The results show that the linear correlation between the electrification degree and the electrostatic current was not good, and the relation between the electrification degree of oil flow and electrostatic current (i) could be expressed as ρ<SUP>(0)</SUP> =0.2049 i<SUP>(0)</SUP> +169.4419 according to grey model GM (0, 2) when the electrification degree of oil flow is represented by the charge number generated from transformer oil per unit volume, namely the charge density (ρ).
Composite action of hollow concrete-filled circular steel tubular stub columns
QIANG FU,Fa-Xing Ding,Tao Zhang,Liping Wang,Chang-jing Fang 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.26 No.6
To better understand the influence of hollow ratio on the hollow concrete-filled circular steel tubular (H-CFT) stub columns under axial compression and to propose the design formula of ultimate bearing capacity for H-CFT stub columns, 3D finite element analysis and laboratory experiments were completed to obtain the load-deformation curves and the failure modes of H-CFT stub columns. The changes of the confinement effect between core concrete and steel tube with different hollow ratios were discussed based on the finite element results. The result shows that the axial stress of concrete and hoop stress of steel tube in H-CFT stub columns are decreased with the increase of hollow ratio. After the yield of steel, the reduction rate of longitudinal stress and the increase rate of circumferential stress for the steel tube slowed down. The confinement effect from steel tube on concrete also weakened slowly with the increase of hollow ratio. Based on the limit equilibrium method, a simplified formula of ultimate bearing capacity for the axially loaded H-CFT stub columns was proposed. The predicted results showed satisfactory agreement with the experimental and numerical results.
Flexural Behavior and Prediction Model of Basalt Fiber/Polypropylene Fiber-Reinforced Concrete
Qiang Fu,Zhaorui Zhang,Wenrui Xu,Xu Zhao,Lu Zhang,Yan Wang,Ditao Niu 한국콘크리트학회 2022 International Journal of Concrete Structures and M Vol.16 No.5
The flexural behavior of basalt fiber (BF)/polypropylene fiber (PF)-reinforced concrete (BPRC) was investigated. When the content of BF and PF is 0.1%, the addition of fibers increases the compressive strength of concrete. A BF content of 0.1% has the most obvious effect on improving the compressive strength, but a hybrid fiber content of 0.2% exhibits a negative effect on the compressive strength. The addition of BF and PF can increase the flexural strength and the expansion tortuosity of the fracture cracks, thus enhancing the ductility of concrete. The hybrid fibers with content of 0.1% are most beneficial to increase the flexural strength. However, the ductility of concrete and the tortuosity of fracture crack decrease with the matrix strength, and the improvement proportion of fibers on the flexural strength also decreases. When the BF and PF are mixed, compared to the case of single fiber added, there is no significant change in the damage of BF, whereas the damage of PF is more severe. The flexural toughness index FTδ effectively characterizes the change in the flexural toughness of BPRC. The hybrid fiber contents of 0.1% and 0.2% exhibit the most significant improving effect on FT-l/600 and FT-l/150, respectively. Considering the influence of fibers on the compressive strength, flexural strength and flexural toughness of concrete, a hybrid content of 0.1% is the optimal choice of fiber content. A prediction model for flexural strength of BPRC is proposed based on the composite material theory.
Qiang Feng,Shenggang Fu,Chengxiang Wang,Weiwei Liu,Ying Wang,Weiguo Qiao 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.9
There is an increasing trend of tunnel infrastructure construction in cold regions due to the advance of a western development strategy. To reduce the frost damage sustained by a tunnel, it is important to consider the mechanical properties of frost heave in the surrounding rock by analyzing the anisotropic frost heave of a cold-region tunnel. This paper elaborates on the behavior of anisotropic frost heave in the rock surrounding a cold-region tunnel, and the coefficient of displacement relief is adopted to indirectly quantify the influence of the tunnel construction process. The analytical elasto-plastic solutions of the stress and deformation in the surrounding rock are derived with Drucker-Prager criteria (hereinafter referred to as the D-P criteria) by modifying the constitutive equation for the frozen surrounding rock. Finally, an example is given to obtain the influence law of the distribution of stress and deformation in the surrounding rock. The results indicate that the plastic radius and frost force calculated by considering isotropic frost heave are greater than those obtained by considering anisotropic frost heave. The influence of the related parameters also analyzed. Some useful insights are provided for future numerical simulations and the design and construction of cold-region tunnels.
Fu, Qiang,Wang, Bai-Chen,Jin, Xiang,Li, Hong-Bing,Han, Pei,Wei, Kai-Hua,Zhang, Xue-Min,Zhu, Yu-Xian Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6
Proteins accumulated in dry, stratified Arabidopsis seeds or young seedlings, totaled 1100 to 1300 depending on the time of sampling, were analyzed by using immobilized pH gradient 2-DE gel electrophoresis. The molecular identities of 437 polypeptides, encoded by 355 independent genes, were determined by MALDI-TOF or TOF-TOF mass spectrometry. In the sum, 293 were present at all stages and 95 were accumulated during the time of radicle protrusion while another 18 appeared in later stages. Further analysis showed that 226 of the identified polypeptides could be located in different metabolic pathways. Proteins involved in carbohydrate, energy and amino acid metabolism constituted to about 1/4, and those involved in metabolism of vitamins and cofactors constituted for about 3% of the total signal intensity in gels prepared from 72 h seedlings. Enzymes related to genetic information processing increased very quickly during early imbibition and reached highest level around 30 h of germination.