Molecular and cytological analyses of A and C genomes at meiosis in synthetic allotriploid Brassica hybrids (ACC) between B.napus (AACC) and B.oleracea (CC)

Yan Yang1,Xiaochun Wei,Gongyao Shi,Fang Wei,Janeen Braynen,Jingshu Zhang,Baoming Tian,Gangqiang Cao,Xiaowei Zhang 한국식물학회 2017 Journal of Plant Biology Vol.60 No.2

Success of interspecific hybridization relies mostly on the adequate similarity between the implicated genomes to ensure synapsis, pairing and recombination between appropriate chromosomes during meiosis in allopolyploid species. Allotetraploid Brassica napus (AACC) is a model of natural hybridization between Brassica rapa (AA) and Brassica oleracea (CC), which are originally derived from a common ancestor, but genomic constitution of the same chromosomes probably varied among these species through time after establishment, giving rise to cytogenetic difference in the synthetic hybrids. Herein we investigated meiotic behaviors of A and C chromosomes of synthetic allotriploid Brassica hybrids (ACC) at molecular and cytological levels, which result from the interspecific cross between natural B. napus (AACC) and B.oleracea (CC), and the results showed that meiosis course was significantly aberrant in allotriploid Brassica hybrids, and chromosomes aligned chaotically at metaphase I, chromosome bridges and lags were frequently observed from later metaphase I to anaphase II during meiosis. Simultaneously, we also noticed that meiosis-related genes were abruptly down-regulated in allotriploid Brassica hybrids, which likely accounted for irregular scenario of meiosis observed in these synthetic hybrids. Therefore, these results indicated that inter-genomic exchanges of A and C chromosomes could occur frequently in synthetic Brassica hybrids, and provided an efficient approach for genetic changes of homeologous chromosomes during meiosis in polyploid B.napus breeding program.

Combining Downscaled Global Climate Model Data with SWAT to Assess Regional Climate Change Properties and Hydrological Responses

Tian Yang,Xiao Yang,Chao Jia,Cong Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.5

Global warming has led to a high incidence of extreme hydrometeorological events. Predicting the spatial and temporal response of water resources to future climate change is necessary for the construction of regional water facilities. In this study, Global Circulation Models (GCMs) and the Soil and Water Assessment Tool (SWAT) were combined with the Delta downscaling method in the Dongyu River Basin (DRB), China. Three different GCMs under two Shared Socioeconomic Pathways (SSPs) 2-4.5 and 3-7.0 scenarios were considered. Relative to the baseline (2008-2018), streamflow under two scenarios was assessed from 2025 to 2064. The results show that the mean annual precipitation would rise by 12.3% and 12.8% under SSP2-4.5 and SSP3-7.0 scenarios, respectively. The mean monthly maximum temperature and minimum temperature would increase in summer but decrease in winter under two scenarios. Future climate change was estimated to increase the mean annual streamflow by 9.00% under SSP2-4.5 scenario and by 6.92% under SSP3-7.0 scenario. The wet season was forecast to arrive earlier and the frequency of extreme events would likely increase in the future. A high positive correlation was projected between the annual precipitation and the annual streamflow, with an average correlation coefficient of 0.76 (p < 0.05). The dominant climate variables would be different on the monthly scale. Streamflow in the dry season would be more susceptible to temperature changes. The findings of this study show how climate change affects streamflow in the DRB and give adaptive management a scientific foundation.

Impact of Latrogenic Biliary Injury during Laparoscopic Cholecystectomy on Surgeon’s Mental Distress A Nationwide Survey from China

( Tian Yang ),( Hai-su Dai ),( Lei Liang ),( Cheng-cheng Zhang ),( Zhang-jun Cheng ),( Yong-hai Peng ),( Yao-ming Zhang ),( Zhi-yu Chen ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Iatrogenic biliary injury (IBI) following laparoscopic cholecystectomy (LC) is the most common and recognized iatrogenic complications. Little is known whether LC-IBI would lead to surgeon’s mental distress. This study reports the incidence of surgeon’s mental distress who have caused LC-IBI and risk factors of surgeon’s severe mental distress (SMD). Methods: A cross-sectional survey in the form of electronic questionnaire was conducted among Chinese general surgeons who have caused LC-IBI. The six collected clinical features relating to mental distress included: 1) feeling burnout, anxiety, or depression, 2) avoiding performing LC, 3) having physical reactions when recalling the incidence, 4) having the urge to quit surgery, 5) taking psychiatric medications, and 6) seeking professional psychological counseling. Univariable and multivariable analyses were performed to identify risk factors of SMD, which was defined as meeting ≥3 of the above-mentioned clinical features. Results: Among 1,466 surveyed surgeons, 1,236(84.3%) experienced mental distress following LC-IBI, and nearly half (49.7%, 614/1236) had SMD. Multivariable analyses demonstrated that surgeons from non-university affiliated hospitals (OR:1.873), patients who required multiple repair operations (OR:4.075), patients who required hepaticojejunostomy/partial hepatectomy (OR:1.859), existing lawsuit litigation (OR:10.491), existing violent doctor-patient conflicts (OR:4.995), needing surgeons’ personal compensation (OR:2.531), and additional administrative punishment by hospitals (OR:2.324) were independent risk factors of surgeon’s SMD. Conclusions: Four out of five surgeons experienced mental distress following LC-IBI, and nearly half had SMD. Several independent risk factors of SMD were identified, which could help to make strategies to improve mental well-being of these surgeons.

Away From the Haze

Tian Yang 한국콘텐츠학회 2013 ICCC International Digital Design Invitation Exhib Vol.2013 No.-

Preparation of Co₃O₄/NF Anode for Lithium-ion Batteries

Tian, Shiyi,Li, Botao,Zhang, Bochao,Wang, Yang,Yang, Xu,Ye, Han,Xia, Zhijie,Zheng, Guoxu The Korean Electrochemical Society 2020 Journal of electrochemical science and technology Vol.11 No.4

Due to its characteristics of light weight, high energy density, good safety, long service life, no memory effect, and environmental friendliness, lithium-ion batteries (LIBs) are widely used in various portable electronic products. The capacity and performance of LIBs largely depend on the performance of electrode materials. Therefore, the development of better positive and negative materials is the focus of current research. The application of metal organic framework materials (MOFs) derivatives in energy storage has attracted much attention and research. Using MOFs as precursors, porous metal oxides and porous carbon materials with controllable structure can be obtained. In this paper, rod-shaped Co-MOF-74 was grown on Ni Foam (NF) by hydrothermal method, and then Co-MOF-74/NF precursor was heat-treated to obtain rodshaped Co₃O₄/NF. Ni Foam was skeleton structured, which effectively relieved. The change of internal stress changes and destroys the structural volume of the electrode material and reduces the capacity attenuation. Co₃O₄/NF composite material has a specific discharge capacity of up to 1858 mA h/g for the first time, and a reversible capacity of up to 902.4 mA h/g at a current density of 200 mA/g, and has excellent rate and impedance performance. The synthesis strategy reported in this article opens the way to design high-performance electrodes for energy storage and electrochemical catalysis.

Aspartate Transaminase to Platelet Ratio Index (APRI) and Albumin-Bilirubin Grade (ALBI) Predict Postoperative Morbidity Following Hepatectomy for Hepatocellular Carcinoma A Multicenter Cohort Study

( Tian Yang ),( Li-yang Sun ),( Lei Liang ),( Ya-hao Zhou ),( Wei-min Gu ),( Hong Wang ),( Ting-hao Chen ),( Yong-yi Zeng ),( Wan Yee Lau ),( Meng-chao Wu ),( Feng Shen ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Postoperative morbidity following hepatectomy remains high, and understanding its risk factors is important to improve perioperative outcomes. We aimed to identify the role of two non-invasive markers - albumin-bilirubin (ALBI) and aspartate transaminase to platelet ratio index (APRI) - in predicting postoperative morbidity following hepatectomy for hepatocellular carcinoma (HCC). Methods: A multicenter data of patients undergoing hepatectomy for HCC at 8 centers were retrospectively analyzed. These patients were divided into normal and high groups according to preoperative ALBI and APRI scores. ALBI and APRI’s predictive accuracy of postoperative 30-day overall and major morbidity were evaluated by the area under the receiver operating characteristic curve (AUC) and compared with two conventional scores: Child-Pugh grade and model for end-stage liver disease (MELD). Results: In 2,301 patients, 866 (37.6%) and 400 (17.4%) were in the high ALBI and APRI groups, respectively. There were significant differences of postoperative overall morbidity between the normal and high ALBI groups (26.2% vs. 40.1%, P<0.001), as well as between the normal and high APRI groups (29.2% vs. 42.4%, P<0.001). The AUCs of the ALBI and APRI scores for predicting overall morbidity are greater than those of Child- Pugh grade and MELD score. Multivariable analyses revealed that ALBI and APRI were independent predictors of overall morbidity in both preoperative and postoperative prediction models. Similar results existed in predicting postoperative major morbidity. Conclusions: Preoperative ALBI and APRI could predict postoperative 30-day overall and major morbidity following hepatectomy for HCC before or after surgery.

Friendship Influence on Mobile Behavior of Location Based Social Network Users

Yang Song,Zheng Hu,Xiaoming Leng,Hui Tian,Kun Yang,Xin Ke 한국통신학회 2015 Journal of communications and networks Vol.17 No.2

In mobile computing research area, it is highly desirable to understand the characteristics of user movement so that the user friendly location aware services could be rendered effectively. Location based social networks (LBSNs) have flourished recently and are of great potential for movement behavior exploration and datadriven application design. While there have been some efforts on user check-in movement behavior in LBSNs, they lack comprehensive analysis of social influence on them. To this end, the socialspatial influence and social-temporal influence are analyzed synthetically in this paper based on the related information exposed in LBSNs. The check-in movement behaviors of users are found to be affected by their social friendships both from spatial and temporal dimensions. Furthermore, a probabilistic model of user mobile behavior is proposed, incorporating the comprehensive social influence model with extent personal preference model. The experimental results validate that our proposed model can improve prediction accuracy compared to the state-of-the-art social historical model considering temporal information (SHM+T), which mainly studies the temporal cyclic patterns and uses them to model user mobility, while being with affordable complexity.

Risk Factors, Patterns and Long-Term Prognosis of Early and Late Recurrence in Patients with Hepatitis B Virus-Associated Hepatocellular Carcinoma

( Tian Yang ),( Ming-da Wang ),( Chao Li ),( Lei Liang ),( Hao Xing ),( Li-yang Sun ),( Bing Quan ),( Han Wu ),( Xin-fei Xu ),( Timothy M ),( Pawlik ),( Wan Yee Lau ),( Feng Shen ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Survival after liver resection of hepatocellular carcinoma (HCC) remains poor due to a high incidence of recurrence. We sought to investigate risk factors, patterns, and long-term prognosis among patients with early and late recurrence after liver resection for hepatitis B virus (HBV)-associated HCC. Methods: Data of consecutive patients undergoing curative resection for HBV-associated HCC were analyzed. According to the time to recurrence after surgery, recurrence was divided into early (≤ 2 years) and late recurrence (> 2 years). Characteristics, patterns of initial recurrence and post-recurrence survival (PRS) were compared between patients with early and late recurrence. Risk factors of early and late recurrence, and predictors of PRS were identified by univariable and multivariable Cox-regression analyses. Results: mong 894 patients, 322 (36.0%) and 282 (31.5%) developed early and late recurrence, respectively. On multivariable analyses preoperative HBV-DNA>104 copies/ml was associated with both early and late recurrence, while postoperative no/ irregular antiviral therapy was associated with late recurrence. Compared with patients with late recurrence, patients with early recurrence had a lower proportion of intrahepatic only recurrence (72.0% vs. 91.1%, P<0.001), as well as a lower chance of receiving potentially-curative treatments for recurrence (33.9% vs. 50.7%, P<0.001) and a worse median PRS (19.1 vs. 37.5 months, P<0.001). Multivariable analysis demonstrated that early recurrence was independently associated with worse PRS (HR 1.361, 95%CI 1.094-1.692, P=0.006). Conclusions: Although risk factors associated with early recurrence and late recurrence were different, a high preoperative HBV-DNA load was an independent hepatitis-related risk for both early and late recurrence. Early recurrence was associated with

LncRNA MALAT1 Depressed Chemo-Sensitivity of NSCLC Cells through Directly Functioning on miR-197-3p/p120 Catenin Axis

Yang, Tian,Li, Hong,Chen, Tianjun,Ren, Hui,Shi, Puyu,Chen, Mingwei Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.3

This study was aimed to explore if lncRNA MALAT1 would modify chemo-resistance of non-small cell lung cancer (NSCLC) cells by regulating miR-197-3p and p120 catenin (p120-ctn). Within this investigation, we totally recruited 326 lung cancer patients, and purchased 4 NSCLC cell lines of A549, H1299, SPC-A-1 and H460. Moreover, cisplatin, adriamycin, gefitinib and paclitaxel were arranged as chemotherapies, and half maximal inhibitory concentration (IC50) values were calculated to evaluate the chemo-resistance of the cells. Furthermore, mice models of NSCLC were also established to assess the impacts of MALAT1, miR-197-3p and p120-ctn on tumor growth. Our results indicated that MALAT1 and miR-197-3p were both over-expressed within NSCLC tissues and cells, when compared with normal tissues and cells (P < 0.05). The A549, H460, SPC-A-1 and SPC-A-1 displayed maximum resistances to cisplatin ($IC50=15.70{\mu}g/ml$), adriamycin ($IC50=5.58{\mu}g/ml$), gefitinib ($96.82{\mu}mol/L$) and paclitaxel (141.97 nmol/L). Over-expression of MALAT1 and miR-197-3p, or under-expression of p120-ctn were associated with promoted viability and growth of the cancer cells (P < 0.05), and they could significantly strengthen the chemo-resistance of cancer cells (P < 0.05). MALAT1 Wt or p120-ctn Wt co-transfected with miR-197-3p mimic was observed with significantly reduced luciferase activity within NSCLC cells (P < 0.05). Finally, the NSCLC mice models were observed with larger tumor size and weight under circumstances of over-expressed MALAT1 and miR-197-3p, or under-expressed p120-ctn (P < 0.05). In conclusion, MALAT1 could alter chemo-resistance of NSCLC cells by targeting miR-197-3p and regulating p120-ctn expression, which might assist in improvement of chemo-therapies for NSCLC.

Experimental Installation of Pressure Oscillation based on Pulse-driving Technique

YANG, Tian-hao,LIU, Pei-jin,JIN, Bing-ning The Society for Aerospace System Engineering 2015 International Journal of Aerospace System Engineer Vol.2 No.1

Under the background of combustion instability in solid rocket motor, to study the relationship between pressure oscillations and dynamic process of propellant flames, it is necessary to simulate an oscillation environment with certain frequency, amplitude and duration. This paper presents an experimental installation of pressure oscillation based on pulse-driving technique, with which pressure oscillations features under different pulse-driving conditions were compared and analyzed. For the pulse-driver applied in this paper, a pressure oscillation with 0.15s-0.5s duration, 179Hz-210Hz first order frequency, 0.04MPa-0.35MPa amplitude is simulated. The test results show that an oscillation with higher frequency and lager amplitude can be obtained when pulse-driver is installed on the top of the installation cavity, while on the side, an oscillation with a longer duration and approximate cavity natural frequency can be simulated.