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Nguyen, Khanh Hoang Viet,Dao, Trong Khoa,Nguyen, Hong Duong,Nguyen, Khanh Hai,Nguyen, Thi Quy,Nguyen, Thuy Tien,Nguyen, Thi Mai Phuong,Truong, Nam Hai,Do, Thi Huyen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.5
Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.
Thuy Duong Nguyen,To Thi Xuan Hang,Thu Thuy Pham,Anh Son Nguyen,Thu Thuy Thai,Gia Vu Pham,Ngoc Bach Ta 한국부식방식학회 2022 Corrosion Science and Technology Vol.21 No.6
Corrosion inhibitors based on Zn-Al hydrotalcites containing benzoate (ZnAlHB) with different molar ratios of Zn/Al were prepared with a co-precipitation process. Compositions and structures of the resulting hydrotalcites were studied with suitable spectroscopic methods such as inductively coupled plasma mass spectrometry (ICP-MS), ultraviolet-visible spectrophotometry (UV-Vis), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and surface zeta potential measurements, respectively. Results of physico-chemical studies showed that crystallite sizes, compositions of products, and surface electrical properties were significantly changed when the molar ratio of Zn/Al was increased. The release of benzoate from hydrotalcites also differed slightly among samples. Anticorrosion abilities of hydrotalcites intercalated with benzoate at a concentration of 3 g/L on carbon steel were analyzed using electrochemical impedance spectroscopy (EIS), polarization curve, energy-dispersive X-ray spectroscopy (EDX), and SEM. Corrosion inhibition abilities of benzoate modified hydrotalcites in 0.1 M NaCl showed an upward trend with increasing Zn/Al ratio. The reason for the dependence of corrosion resistance on the Zn/Al ratio was discussed, including changes in the microstructure of hydrotalcites such as crystal size, density, uniformity, and formation of ZnO.
( Thuy Duong Nguyen ),( Thu Thuy Pham ),( Anh Son Nguyen ),( Ke Oanh Vu ),( Gia Vu Pham ),( To Thi Xuan Hang ) 한국부식방식학회 2023 Corrosion Science and Technology Vol.22 No.1
This work studied the inhibitory effect of the combination of benzoate-intercalated hydrotalcite (HT-BZ) and Ce<sup>3+</sup>-loaded clay (Clay-Ce) on carbon steel (CS). HT-BZ was prepared by the co-precipitation method and Clay-Ce was fabricated by a cation exchange reaction. HT-BZ and Clay-Ce were assessed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) coupled with zeta potential measurement. Electrochemical measurements coupled with scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDX) were used for studying the inhibitory action of the mixture of HT-BZ and Clay-Ce on steel electrodes immersed in 0.1 M NaCl. For comparison, the inhibitory effect of HT-BZ or Clay-Ce alone was also evaluated. The results showed that HT-BZ combined with Clay-Ce provided synergistic inhibition of the CS substrate. The mixture of 0.5 g/L HT-BZ + 0.5 g/L Clay-Ce provided 93.5% inhibition efficiency. The protective mechanism of the HT-BZ + Clay-Ce mixture consisted of the reaction of released BZ and Ce<sup>3+</sup> and the deposition of HT-BZ and Clay-Ce structures on the CS substrate.
Morphological effect of TiO2 catalysts on photocatalytic degradation of methylene blue
Thuy-Duong Nguyen-Phan,Eun Woo Shin 한국공업화학회 2011 Journal of Industrial and Engineering Chemistry Vol.17 No.3
In our previous study, we reported a simple hydrothermal preparation of TiO_2 materials using concentrated hydrochloric acid and controlled the morphology of TiO2 by changing the hydrochloric acid amounts. In this study, we employed the TiO_2 materials with different morphologies in the photocatalytic degradation of methylene blue. In the photocatalytic results, the TiO_2 particles with the prisms and flower-like morphology (DTC-5.0) exhibited the highest photocatalytic activity with kapp = 12.33 × 10^3 min1 due to the synergetic effect of morphology and mixed-phase crystal of TiO_2.
Nguyen-Phan, Thuy-Duong,Nguyen-Huy, Chinh,Woo Shin, Eun Elsevier 2014 Materials letters Vol.131 No.-
<P><B>Abstract</B></P> <P>In this study, the morphological evolution of hierarchical nickel titanates was first-ever investigated by simply raising the solvothermal temperature. At room temperature, irregular aggregates of anatase TiO<SUB>2</SUB> nanoparticles were reluctantly formed. Increasing the temperature to 100°C resulted in the assembly of 3D drum-shaped nickel titanate clusters where the three-dimensional geometry was constructed via coalescent nanoparticles with a mean size of 126.8nm. An intermediate stage containing circular cylinders and irregular prisms was obtained at intermediate temperatures. Further elevation of the solvothermal temperature to 160°C and 180°C gave 1D uniform hexagonal nanoprisms of nickel titanates. Increasing the solvothermal temperature accelerates the nucleation rate, which drastically promotes crystallization and self-assembly, thereby evolving to the hierarchical structure of NiTiO<SUB>3</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Morphological evolution of NiTiO<SUB>3</SUB> was investigated by solvothermal temperatures. </LI> <LI> At room temperature, irregular aggregates of anatase TiO<SUB>2</SUB> nanoparticles were formed. </LI> <LI> 3D drum-shaped NiTiO<SUB>3</SUB> clusters were synthesized via small nanoparticles at 100°C. </LI> <LI> Circular cylinders and irregular prisms of NiTiO<SUB>3</SUB> were obtained at 130°C. </LI> <LI> 1D uniform hexagonal NiTiO<SUB>3</SUB> nanoprisms were constructed at 160°C and 180°C. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Duong Bich Thuy,Duong Minh Cuong,Campbell James,Nguyen Van Minh Hoang,Nguyen Huu Hien,Bui Thi Bich Hanh,Nguyen Van Vinh Chau,McLaws Mary-Louise 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.3
Little is known about antibiotic-resistant Gram-negative bacteria (GNB) intestinal carriage among healthcare workers (HCWs) in Vietnam. All HCWs at a tertiary intensive care units were asked to undertake weekly rectal swabs. Among 40 participants, 65% (26/40) carried extended spectrum β-lactamases (ESBL)/AmpC β-lactamase-producing Escherichia coli. Two HCWs colonized with ESBL/AmpC β-lactamase-producing Klebsiella pneumoniae. One HCW colonized with Acinetobacter baumannii. No one carried Pseudomonas spp.. A quarter (10/40) of HCWs were identified as persistent and frequent carriers. There is an urgent need to screen antibiotic-resistant GNB among HCWs and improve HCWs’ hand hygiene compliance to reduce the transmission of antibiotic-resistant GNB in the hospital.
Nguyen-Phan, Thuy-Duong,Shin, Eun Woo,Pham, Viet Hung,Kweon, Hyukmin,Kim, Sunwook,Kim, Eui Jung,Chung, Jin Suk The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.38
<P>In this study, a new system of integrated photocatalytic adsorbents (IPCAs), three-dimensional wormhole-like mesoporous titanosilicate/reduced graphene oxide layered composites, were prepared with various Ti/Si ratios. The reduced graphene oxide (RGO) nanosheets played the role of giant two-dimensional (2D) platforms for the deposition, spreading and growth of mesostructured layers, leading to a significant enhancement in mesoporosity. The substitution of titanium species into the mesoporous silica/RGO significantly changed the textural, physicochemical and optical properties. Gradually increasing the Ti doping content dramatically promoted the adsorptivity and UV-photocatalytic degradation of the dye contaminant; however, an excess dosage (20 mol%) inhibited the UV activity. This could be attributed to the changes in the coordination geometry and electronic states of Ti species in the composites. The IPCAs that dominantly contained highly dispersed and isolated Ti-oxide species in tetrahedral coordination within a mesostructured framework exhibited much higher photoactivity than those composed of aggregated and octahedrally coordinated Ti species due to the formation of efficient charge-transfer excited states.</P> <P>Graphic Abstract</P><P>Mesoporous titanosilicate/reduced graphene oxide layered composites exhibited good adsorptivity and photodegradation of a dye contaminant. Highly dispersed and isolated tetrahedral Ti-oxide species in the mesostructured framework were beneficial to photoreactivity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm33309k'> </P>