인체 대장암 세포주 SW480에서 docosahexaenoic acid에 의한 침윤억제 기전

Soyeon Shin(신소연),Yong-Jo Kim(김용조),Kyoung-Sub Song(송경섭),Kaipeng Jing,Nayeong Kim(김나영),Soyeon Jeong(정소연),Ji-Hoon Park(박지훈),Kang-Sik Seo(서강식),Jun-Young Heo(허준영),Hyun-Joo Kwon(권현주),Jong-Il Park(박종일),Seung 한국생명과학회 2010 생명과학회지 Vol.20 No.4

대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 ω3-PUFAs를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 ω3-PUFAs는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA 등 ω3-PUFA의 항침윤 기전을 규명하여 다음과 같은 결과를 얻었다. DHA 및 EPA는 대장암 세포주 SW480의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었으며 TUNEL assay로 apoptotic cell death가 확인 되었다. DHA는 β-catenin 단백 및 TCF/LEF luciferase 활성을 농도 의존적으로 억제 하였다. SW480 세포의 침윤능은 DHA의 농도에 의존적으로 억제되었다. DHA처리 후 MMP-9 및 MMP-2 mRNA양이 감소되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제되었다. NF-kB 및 p-IkB 단백짙양도 DHA의 처리농도에 의존적으로 감소하였으며 NF-kB promoter의 활성도 억제되었다. 이상의 결과로 ω3-PUFA는 대장암에서 NF-kB 신호전달 차단에 의한 MMP-2 및 MMP-9 발현을 억제하여 침윤을 억제하여 항암작용을 나타낼 수 있음을 시사하며, 따라서 ω3-PUFA는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다. Colon cancer is one of the most common malignancies in the western world and the second leading cause of cancer death in Korea. Epidemiology studies have shown a reduced incidence of colon cancer among populations consuming a large quantity of ω3-polyunsaturated fatty acids (ω3-PUFA) of marine origin. Recently, it has been found that ω3-PUFA has an antineoplastic effect in several cancers. This study was designed to investigate the mechanism of the anti-invasive effect of ω3-PUFA in colon cancer. ω3-PUFA, docosahexaenoic acids (DHA) and eicosapentaenoic acid (EPA) treatment resulted in a dose-dependent inhibition of cell growth in SW480 human colon cancer cells. In contrast, arachidonic acid (AA), a ω6-PUFA, exhibited no significant effect. This action likely involves apoptosis, given that DHA treatment increased apoptotic cells in TUNEL assay. Moreover, invasiveness of SW480 cells was inhibited following treatment of DHA in a dose-dependent manner; in contrast, AA had no effect. The levels of MMP-9 and MMP-2 mRNA decreased after DHA pretreatment. MMP-9 and MMP-2 promoter activities were also inhibited by DHA treatment. The levels of NF-kB and p-IkB protein were down-regulated by DHA pretreatment in a dose dependent manner. In addition, DHA inhibited NF-kB promoter reporter activities. These findings suggest that ω3-PUFA may inhibit cancer cell invasion by inhibition of MMPs via reduction of NF-kB in colon cancer. In conclusion, ω3-PUFA could be used for chemoprevention and treatment of human colon cancer.

Development of cellulose hydrogel microsphere for lipase immobilization

Soyeon JO,Saerom PARK,Sanghyun LEE 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.10

Development of Cellulose Hydrogel Microspheres for Lipase Immobilization

Soyeon Jo,Sae-Rom Park,Yujin Oh,Jiyeon Hong,Hyung Joo Kim,Kwang Jin Kim,Kyeong Keun Oh,이상현 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.1

Cellulose hydrogel microspheres were prepared by sol-gel transition using an ionic liquid-in-oil emulsion. Factors that influenced the formation of these microspheres, including the ratio of ionic liquid to oil, surfactant concentration, and stirring speed, were optimized for lipase immobilization. Using the optimized method, Candida rugosa lipase was efficiently immobilized on the microspheres by physical adsorption. As compared with the free lipase, the specific activity of the immobilized lipase was 1.4 times higher, its half-life at 45°C was 41 times longer, and it showed an enhanced stability over a wide pH range. The lipase immobilized on cellulose microspheres showed a much higher loading efficiency, immobilization yield, and specificity constant than lipase immobilized on microcrystalline cellulose or millimeter-sized hydrogel beads. To increase the reusability of cellulose microspheres as an enzyme support material, magnetic cellulose microspheres were also prepared by adding Fe3O4. The lipase immobilized on magnetic cellulose microspheres was simply recovered using a magnet and continuously reused with a minimal loss of activity.

Impact of Body Mass Index on Survival Depending on Sex in 14,688 Patients with Gastric Cancer in a Tertiary Hospital in South Korea

Jo Hyeong Ho,Kim Nayoung,Jang Jieun,Choi Yonghoon,Park Jaehyung,Park Young Mi,Ahn Soyeon,Yoon Hyuk,Shin Cheol Min,Park Young Soo,Lee Dong Ho,Oh Hyeon Jeong,Lee Hye Seung,Park Young Suk,Ahn Sang-Hoon,S 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.2

Background/Aims: The incidence and prognosis of gastric cancer (GC) shows sex difference. This study aimed to evaluate the effect of body mass index (BMI) on GC survival depending on sex. Methods: The sex, age, location, histology, TNM stages, BMI, and survival were analyzed in GC patients from May 2003 to February 2020 at the Seoul National University Bundang Hospital. Results: Among 14,688 patients, there were twice as many males (66.6%) as females (33.4%). However, under age 40 years, females (8.6%) were more prevalent than males (3.1%). Cardia GC in males showed a U-shaped distribution for underweight (9.6%), normal (6.4%), overweight (6.1%), obesity (5.6%), and severe obesity (9.3%) but not in females (p=0.003). Females showed decreased proportion of diffuse-type GC regarding BMI (underweight [59.9%], normal [56.8%], overweight [49.5%], obesity [44.8%], and severe obesity [41.7%]), but males did not (p<0.001). Both sexes had the worst prognosis in the underweight group (p<0.001), and the higher BMI, the better prognosis in males, but not females. Sex differences in prognosis according to BMI tended to be more prominent in males than in females in subgroup analysis of TNM stages I, II, and III and the operative treatment group. Conclusions: GC-specific survival was affected by BMI in a sex-dependent manner. These differences may be related to genetic, and environmental, hormonal factors; body composition; and muscle mass (Trial registration number: NCT04973631).

Frequency and Type of Disputed rpoB Mutations in Mycobacterium tuberculosis Isolates from South Korea

Jo, Kyung-Wook,Lee, Soyeon,Kang, Mi Ran,Sung, Heungsup,Kim, Mi-Na,Shim, Tae Sun The Korean Academy of Tuberculosis and Respiratory 2017 Tuberculosis and Respiratory Diseases Vol.80 No.3

Background: A disputed rpoB mutation is a specific type of rpoB mutation that can cause low-level resistances to rifampin (RIF). Here, we aimed to assess the frequency and types of disputed rpoB mutations in Mycobacterium tuberculosis isolates from South Korea. Methods: Between August 2009 and December 2015, 130 patients exhibited RIF resistance on the MTBDRplus assay at Asan Medical Center. Among these cases, we identified the strains with disputed rpoB mutation by rpoB sequencing analysis, as well as among the M. tuberculosis strains from the International Tuberculosis Research Center (ITRC). Results: Among our cases, disputed rpoB mutations led to RIF resistance in at least 6.9% (9/130) of the strains that also exhibited RIF resistance on the MTBDRplus assay. Moreover, at the ITRC, sequencing of the rpoB gene of 170 strains with the rpoB mutation indicated that 23 strains (13.5%) had the disputed mutations. By combining the findings from the 32 strains from our center and the ITRC, we identified the type of disputed rpoB mutation as follows: CTG511CCG (L511P, n=8), GAC516TAC (D516Y, n=8), CTG533CCG (L533P, n=8), CAC526CTC (H526L, n=4), CAC526AAC (H526N, n=3), and ATG515GTG (M515V, n=1). Conclusion: Disputed rpoB mutations do not seem to be rare among the strains exhibiting RIF resistance in South Korea.

Adhesive protein-based Janus particles for multifunctional drug delivery

Soyeon JEONG,Yeonsu JEONG,Yun Kee JO,Hyung Joon CHA 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Software-defined home networking devices for multi-home visual sharing

Jinyong Jo,Soyeon Lee,JongWon Kim IEEE 2014 IEEE transactions on consumer electronics Vol.60 No.3

<P>Existing home-networking protocols do not robustly incorporate universal connectivity among multiple homes, which leaves their use restricted to a single home. In addition, even in a single home network, new functional requirements ask for more diversified forms of networking control. This paper presents in-home consumer electronic devices that incorporate the emerging SDN (Software Defined Networking) paradigm. The proposed devices enable ondemand provisioning for protocol-agnostic home networking and thus provide a high degree of flexibility for intra-home networking as well as wider connectivity for inter-home networking. The feasibility of the prototype devices is verified by realizing a multi-home visual-sharing scenario and by supporting diverse future scenarios.</P>

Draft genomes of two morphologically similar mushrooms endemic in Korea; Ganoderma lingzhi and Perenniporia fraxinea

Hyun Jo Koo,Eun-Kyung Kim,Hwa-Yong An,Soyeon Kang,Gyoungju Nah 한국육종학회 2023 한국육종학회 공동학술발표집 Vol.2023 No.-

오메가-3 지방산에 의한 COX-2/MMPs/VEGF 억제에 따른 대장암세포의 종양 형성 및 침윤 억제

신소연(Soyeon Shin),김용조(Yong-Jo Kim),한승현(Seung-Hyeon Han),프라산타(Prashanta Silwal),허준영(Jun-Young Heo),전영주(Young-Joo Jeon),박승길(Seung-Kiel Park),권기량(Gi-Ryang Kweon),박종일(Jong-Il Park),임규(Kyu Lim) 한국생명과학회 2017 생명과학회지 Vol.27 No.9

대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 오메가-3를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 오메가-3는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA의 항침윤, 항혈관 신생 및 항종양 형성능 억제의 기전을 규명하여 다음과 같은 결과를 얻었다. DHA는 인체 대장암 세포주 HT29 의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었다. FACS 분석에서 DHA 처리했을 때 Sub G1 phase의 세포가 DHA의 농도 의존적으로 증가 하였다. DHA 처리 후 cleaved PARP가 증가하고, uncelaved caspase-3가 감소 하였다. HT29 세포의 침윤능은 DHA 처리에 의해 억제 되었다. DHA 처리 후 MMP-9 및 MMP-2 mRNA양이 감소 되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제하였으며 VEGF promoter 활성도 DHA에 의해 억제 되었다. NF-kB promoter 활성 및 핵으로의 이동도 DHA에 의해 억제 되었다. In vivo 동물실험에서 생쥐 대장암 세포주인 MCA38에 대한 Fat-1 transgenic mice에서의 종양 형성능은 현저히 억제 되었다. 면역형광염색법을 이용한 Fat-1 transgenic mice의 종양 조직에서의 TUNEL 양성세포는 wild type mice에 비해 현저히 증가하였으나 CD31의 형광강도는 감소 되었다. 이상의 결과로 오메가-3는 대장암 세포에서 NF-kB 억제에 따른 COX-2, MMP-2 및 MMP-9 등 matrix matalloproteinase의 억제를 통한 침윤능의 억제, VEGF 억제를 통한 혈관신생의 억제등 복합적 기전에 의해 항암작용을 나타내리라 생각되며, 따라서 오메가-3는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다. Epidemiology studies have reported a reduced incidence of colon cancer among populations that consume a large quantity of ω3-polyunsaturated fatty acids (ω3-PUFAs) of marine origin. Herein, we demonstrated a mechanism of anticancer action of ω3-PUFAs, showing that they suppressed invasion and tumorigenicity in colon cancer cells. Docosahexaenoic acids (DHA) inhibited the cell growth of HT29 cells. This action likely involved apoptosis, given that the DHA treatment increased the cleaved form of PARP and sub G1 cells. Moreover, the invasiveness of HT29 cells was inhibited following DHA treatment, whereas arachidonic acid (AA) had no effect. The levels of Matrix-metalloproteinase-9(MMP-9) and MMP-2 mRNA decreased after DHA pretreatment. DHA treatment inhibited MMP-9 and MMP-2 promoter activities and reduced VEGF promoter activity. DHA pretreatment also inhibited the activities of prostaglandin-2 (PGE2)-induced MMPs and the VEGF promoter. Cyclooxygenase-2(COX-2) overexpression increased the activity of MMPs and that of the Vascular endotherial growth factor (VEGF) promoter in HT29 cells, and DHA inhibited NF-kB and COX-2 promoter reporter activities. As shown by in vivo experiments, when mouse colon cancer cells (MCA38) were implanted into Fat-1 and wild-type mice, both the tumoral size and volume were dramatically inhibited in Fat-1 transgenic mice. Furthermore, TUNEL-positive cells increased in tumors from Fat-1 mice compared with wild mice. In immunohistochemistry, the intensity of CD31 in Fat-1 tumors was weaker. These findings suggest that ω3-PUFAs may inhibit tumorigenicity and angiogenesis as well as cancer cell invasion by suppression of COX-2, MMPs and VEGF via the reduction of NF-kB in colon cancer.

얼굴 검출과 스타일 전이를 활용한 캐릭터 영상 생성 시스템

나소연(Soyeon Na),장소영(Soyoung Chang),조수연(Suyeon Jo),장주용(Ju Yong Chang) 한국방송·미디어공학회 2023 한국방송공학회 학술발표대회 논문집 Vol.2023 No.11

본 논문에서는 StyleGAN2 모델과 Photo2cartoon 모델을 활용하여 사용자의 실제 영상을 캐릭터 스타일 또는 캐리커처 스타일로 변환하는 시스템을 제안한다. 또한, 사용자들이 만든 자신의 가상 캐릭터 프로필의 사용이 용이하도록 웹사이트에서 원하는 기능을 선택하여 영상을 변환하고 제공하여 접근성을 높이는 과정을 제시한다. 본 프로젝트의 최종 목표는 메타버스 시대의 흐름에 맞는 개성 있는 캐릭터 프로필과 캐리커처 프로필을 손쉽게 제작할 수 있는 기술을 제공하는 것이다.