Novel Polyhydroxybutyrate-Degrading Activity of the Microbulbifer Genus as Confirmed by Microbulbifer sp. SOL03 from the Marine Environment

( Sol Lee Park ),( Jang Yeon Cho ),( Su Hyun Kim ),( Hong-ju Lee ),( Sang Hyun Kim ),( Min Ju Suh ),( Sion Ham ),( Shashi Kant Bhatia ),( Ranjit Gurav ),( See-hyoung Park ),( Kyungmoon Park ),( Yun-go 한국미생물 · 생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.1

Ever since bioplastics were globally introduced to a wide range of industries, the disposal of used products made with bioplastics has become an issue inseparable from their application. Unlike petroleum-based plastics, bioplastics can be completely decomposed into water and carbon dioxide by microorganisms in a relatively short time, which is an advantage. However, there is little information on the specific degraders and accelerating factors for biodegradation. To elucidate a new strain for biodegrading poly-3-hydroxybutyrate (PHB), we screened out one PHB-degrading bacterium, Microbulbifer sp. SOL03, which is the first reported strain from the Microbulbifer genus to show PHB degradation activity, although Microbulbifer species are known to be complex carbohydrate degraders found in high-salt environments. In this study, we evaluated its biodegradability using solid- and liquid-based methods in addition to examining the changes in physical properties throughout the biodegradation process. Furthermore, we established the optimal conditions for biodegradation with respect to temperature, salt concentration, and additional carbon and nitrogen sources; accordingly, a temperature of 37℃ with the addition of 3% NaCl without additional carbon sources, was determined to be optimal. In summary, we found that Microbulbifer sp. SOL03 showed a PHB degradation yield of almost 97% after 10 days. To the best of our knowledge, this is the first study to investigate the potent bioplastic degradation activity of Microbulbifer sp., and we believe that it can contribute to the development of bioplastics from application to disposal.

MEGAS : 메타지노믹 데이터 분석 시스템

박솔빈 ( Sol-been Park ),김동욱 ( Dong-wook Kim ),백두권 ( Doo-kwon Baik ) 한국정보처리학회 2013 한국정보처리학회 학술대회논문집 Vol.20 No.1

최근 시퀀싱 기술의 발전으로 생물 데이터는 폭발적으로 증가되고 있으며 이로 인하여 우리는 지놈 시대를 지나 특정 환경 내에 존재하는 생물체들의 총체적인 지놈을 다루는 메타지놈 시대에 살고 있다. 그러나 메타지노믹 데이터의 증가속도에 비해 이를 효율적으로 분석, 검토, 판별할 수 있는 파이프라인 형태의 프로그램은 전무한 실정이다. 또한, 기존 프로그램은 대부분의 관련연구자들이 다루기 힘든 리눅스 환경의 커맨드 라인 방식이며 대용량 데이터를 처리할 수 없는 제한과 처리 속도의 한계성을 가지고 있다. 이러한 문제점들을 해결하고자 윈도우 환경의 직관적인 그래픽 사용자 인터페이스로 유기적인 데이터 분석이 가능한 메타지노믹 데이터 분석 파이프라인을 설계하고 구현하였다.

Medical Microrobot - Wireless Manipulation of a Drug Delivery Carrier through an External Ultrasonic Actuation: Preliminary Results

Han Sol Lee,Gwangjun Go,Eunpyo Choi,Byungjeon Kang,Jong-Oh Park,김창세 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.1

To achieve precise and untethered clinical therapeutics, microrobots have been widely researched. However, because conventional microrobot actuation is based on magnetic forces generated by a magnetic field and magnetic particles, unexpected side effects caused by additional magnetic ingredients could induce clinical safety issues. In this paper, as an alternative to an untethered actuator, we present a novel ultrasonic actuation mechanism that enables drug particle/cell manipulation and micro/nano-robot actuation in clinical biology and medicine. Firstly, characteristics of the acoustic field in the vessel mimic circular tube, formed from particles emerging through a submerged ultrasonic transducer, are mathematically analyzed and modeled. Thereafter, a control method is proposed for trapping and moving the micro-particles by using acoustic radiation force (ARF) in a standing wave of a tangential standing wave. The feasibility of the proposed method could be demonstrated with the help of experiments conducted using a single transducer with a resonance frequency of 950 kHz and a motorized linear stage, which were used in a water tank. The micro-particles in the tube were trapped via ultrasound and the position of the micro-particles could be controlled by frequency manipulation of the transducer and motor control. This study shows that ultrasonic manipulation can be used for specific applications, such as the operation of a micro robot inserted in a peripheral blood vessel and targeted for drug delivery.

Evaluation of the Interaural Attenuation for Pure Tone Audiometry in Korean

Kye Hoon Park,Young Joo Park,Go-Eun Jun,Sol Kil Oh,Chi-Kyou Lee 대한청각학회 2011 Journal of Audiology & Otology Vol.15 No.1

Background and Objectives: The masking should be used in pure tone audiometry when the difference between the air-conduction threshold of the tested ear and the bone-conduction threshold of the non-tested ear is more than interaural attenuation (IA). But the standardized average value of IA in Korean has not been evaluated until now. The purpose of this study is to evaluate the interaural attenuation in Korean population. Subjects and Methods: Of the patients who visited our department from March, 2007 to August, 2009, 44 patients were selected who had unilateral severe sensorineural hearing loss or deafness and contralateral normal hearing. When the difference between air-conduction threshold with and without masking was more than 10 dB, the interaural attenuation at each frequency was obtained by subtracting the contralateral bone conduction from the air-conduction threshold without masking. Results: The interaural attenuations at each frequency were 45-75 dB (250 Hz), 50-75 dB (500 Hz), 40-75 dB (1 kHz), 45-85 dB (2 kHz), 50-90 dB (4 kHz). 77.2% of the subjects showed interaural attenuation of 55-65 dB at 250 Hz, and 80.9% in 50-60 dB at 500 Hz, 79.5% in 55-65 dB at 1 kHz, 83.4% in 45-60 dB at 2 kHz, and 76.4% in 50-65 dB at 4 kHz. Most of the patients were distributed 15-25 dB higher than the minimal interaural attenuation at 1 kHz. At 2 kHz and 4 kHz, most of the patients were distributed around 15 dB of the minimal interaural attenuation. Conclusions: These results can be used as reference values for masking of pure tone audiometry. But, further studies with large population should be done to get more precise results and contributing factors to IA values.

Effects of Piceatannol on Microglia Activation, Amyloidogenesis and Synaptic Plasticity in the Conditions of Hyeprglycemia-Induced Alzheimer’s Disease

Hee Yun Cha,Sol Been Park,Yeo Gyeong Han,Soo Jin Yang 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

중저분자 키토산이 인간암세포 저온저장에 미치는 효과

김정균 ( Jeong Gyun Kim ),오규석 ( Gyu Suk O ),고용훈 ( Yong Hun Go ),장솔지 ( Sol Ji Jang ),박제권 ( Jae Kweon Park ),김대영 ( Daeyoung Kim ),신수영 ( Su Young Shin ),황유진 ( You Jin Hwang ) 한국키틴키토산학회 2013 한국키틴키토산학회지 Vol.18 No.4

Although cryopreservation has been used to keep viability of cells, it has a severe problem with damaging cells while freezing and thawing them. So we have tried long-term reservation of cells to solve the problem. In this study, we stored three kinds of cells (Hep3B, A549, 293T) in the presence of three different medium molecular weight chitosans (MMWCs) in concentration of 0.05%, 0.01%, 0.005% and 0.001%, respectively, under reservation for a certain period of time. We named them P (MW: 7.6 kDa), B (MW: 5.6 kDa), S (MW: 3.5 kDa) respectively. Then we measured viability of cells with FACS and ELISA after 2, 3 and 4 days respectively. And we measured proliferation of cells with cell proliferation ELISA, BrdU (colorimetric). Our data suggest that viability of cells increase in concentration dependent manner of MMWCs. Subsequently this study demonstrated that MMWCs (MW: 3~7 kDa)are significant factors acting in long-term reservation of cells. However MMWCs have no clear effect on proliferation of cells. Further studies are needed to perform in long-term storage of cells using MMWCs including other polysaccharides to enhance an efficiency of storage.

Measuring the Burden of Disease in Korea, 2008-2018

Jung, Yoon-Sun,Kim, Young-Eun,Park, Hyesook,Oh, In-Hwan,Jo, Min-Woo,Ock, Minsu,Go, Dun-Sol,Yoon, Seok-Jun The Korean Society for Preventive Medicine 2021 예방의학회지 Vol.54 No.5

The study aims to examine the current status and differences in the burden of disease in Korea during 2008-2018. We calculated the burden of disease for Koreans from 2008 to 2018 using an incidence-based approach. Disability adjusted life years (DALYs) were expressed in units per 100 000 population by adding years of life lost (YLLs) and years lived with disability (YLDs). DALY calculation results were presented by gender, age group, disease, region, and income level. To explore differences in DALYs by region and income level, we used administrative district and insurance premium information from the National Health Insurance Service claims data. The burden of disease among Koreans showed an increasing trend from 2008 to 2018. By 2017, the burden of disease among men was higher than that among women. Diabetes mellitus, low back pain, and chronic lower respiratory disease were ranked high in the burden of disease; the sum of DALY rates for these diseases accounted for 18.4% of the total burden of disease among Koreans in 2018. The top leading causes associated with a high burden of disease differed slightly according to gender, age group, and income level. In this study, we measured the health status of Koreans and differences in the population health level according to gender, age group, region, and income level. This data can be used as an indicator of health equity, and the results derived from this study can be used to guide community-centered (or customized) health promotion policies and projects, and for setting national health policy goals.

무작위 섬유제거기법을 이용한 구형 입자를 갖는 복합재료의 통계적인 기계적 물성평가

김도원(Do-Won Kim),고명석(Myeong-Seok Go),최한솔(Han-Sol Choi),박신무(Sin-Mu Park),임재혁(Jae Hyuk Lim) 대한기계학회 2019 대한기계학회 춘추학술대회 Vol.2019 No.2

GS-KG9 ameliorates diabetic neuropathic pain induced by streptozotocin in rats

Lee, Jee Youn,Choi, Hae Young,Park, Chan Sol,Pyo, Mi Kyung,Yune, Tae Young,Kim, Go Woon,Chung, Sung Hyun The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.1

Background: Diabetic neuropathy is one of the most devastating ailments of the peripheral nervous system. Neuropathic pain develops in ~30% of diabetics. Here, we examined the suppressive effect of GS-KG9 on neuropathic pain induced by streptozotocin (STZ). Methods: Hyperglycemia was induced by intraperitoneal injection of STZ. Rats showing blood glucose level > 250 mg/dL were divided into five groups, and treatment groups received oral saline containing GS-KG9 (50 mg/kg, 150 mg/kg, or 300 mg/kg) twice daily for 4 wk. The effects of GS-KG9 on pain behavior, microglia activation in the lumbar spinal cord and ventral posterolateral (VPL) nucleus of the thalamus, and c-Fos expression in the dorsal horn of the lumbar spinal cord were examined. Results: The development of neuropathic pain began at Day 5 and peaked at Week 4 after STZ injection. Mechanical and thermal pains were both significantly attenuated in GS-KG9-treated groups from 10 d after STZ injection as compared to those in the STZ control. GS-KG9 also repressed microglia activation in L4 dorsal horn and VPL region of the thalamus. In addition, increase in c-Fos-positive cells within L4 dorsal horn lamina I and II of the STZ control group was markedly alleviated by GS-KG9. Conclusion: These results suggest that GS-KG9 effectively relieves STZ-induced neuropathic pain by inhibiting microglial activation in the spinal cord dorsal horn and VPL region of the thalamus.

GS-KG9 ameliorates diabetic neuropathic pain induced by streptozotocin in rats

Jee Youn Lee,Hae Young Choi,Chan Sol Park,Mi Kyung Pyo,Tae Young Yune,Go Woon Kim,Sung-Hyun CHUNG 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.1

Background: Diabetic neuropathy is one of the most devastating ailments of the peripheral nervous system. Neuropathic pain develops inw30% of diabetics. Here, we examined the suppressive effect of GSKG9 on neuropathic pain induced by streptozotocin (STZ). Methods: Hyperglycemia was induced by intraperitoneal injection of STZ. Rats showing blood glucose level > 250 mg/dL were divided into five groups, and treatment groups received oral saline containing GS-KG9 (50 mg/kg, 150 mg/kg, or 300 mg/kg) twice daily for 4 wk. The effects of GS-KG9 on pain behavior, microglia activation in the lumbar spinal cord and ventral posterolateral (VPL) nucleus of the thalamus, and c-Fos expression in the dorsal horn of the lumbar spinal cord were examined. Results: The development of neuropathic pain began at Day 5 and peaked at Week 4 after STZ injection. Mechanical and thermal pains were both significantly attenuated in GS-KG9-treated groups from 10 d after STZ injection as compared to those in the STZ control. GS-KG9 also repressed microglia activation in L4 dorsal horn and VPL region of the thalamus. In addition, increase in c-Fos-positive cells within L4 dorsal horn lamina I and II of the STZ control group was markedly alleviated by GS-KG9. Conclusion: These results suggest that GS-KG9 effectively relieves STZ-induced neuropathic pain by inhibiting microglial activation in the spinal cord dorsal horn and VPL region of the thalamus.