Aging 조건에 의한 SBA-15의 Morphological Control

천종현,김시재,박동호 인제대학교 2003 仁濟論叢 Vol.18 No.1

중간세공 분자체 SBA-15는 tetraethoxysilane을 실리카원으로 하여 triblock copolymer를 구조유지 시약으로 사용하여 합성된다. 이러한 중간 세공 분자체의 응용성은 역시 결정의 외관상 형태에 의 해 좌우되어진다. 규칙적인 모양의 입자를 합성하는 것은 나노 공간의 SBA-15를 이용한 나노물질을 제조하는데 응용되기 때문에 매우 중요하다. SBA-15의 형태는 숙성과정의 온도와 시간을 바꿔 줌으로써 조절된다. 특히 rod 형태의 입자의 길이는 숙성 반응과 수열반응의 조합에 의해 조절된다. Morphology 변화는 형태학적 재 구조화에 의해 설명될 수 있다. Mesoporous molecular sieve SBA-15 using triblock copolymer as a structure directing agent and tetraethoxysilane as a silicon source has been synthesized. The applicability of mesoporous molecular sieve depends also on the external morphology of crystallites. The synthesis of regular shaped particle is very important due to the application for the preparation of nano materials using the nano space of SBA-15. By varying the aging temperature and duration, the morphology of SBA-15 was controlled Especially, the length of rod-shaped particle was controlled with the combination of reaction condition of aging and hydrothermal reaction. The change of morphology could be attribute to the metamorphic reconstruction

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

[PB-0047] Inhibition of gene expression for anti-nutritional factors and allergen in soybean seeds via RNAi-mediated co-transformation

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Da Hyeon Ha(Da Hyeon Ha),Hye Jeong Kim(Hye Jeong Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0046] Overexpression of IbMYB1a gene induced anthocyanin accumulation in soybean seeds

Si Hyeon Kim(Si Hyeon Kim),Wan Woo Yeom(Wan Woo Yeom),Hye Jeong Kim(Hye Jeong Kim),Hae Gyun Park(Hae Gyun Park),Hye Min Park(Hye Min Park),Cha Young Kim(Cha Young Kim),Young-Soo Chung(Young-Soo Chung) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

일부 성인의 치과위생사의 이미지에 미치는 영향요인

박시현(Si-Hyeon Park),강민이(Min-i Kang),김다현(Da-Hyun Kim),김윤희(Yun-Hee Kim),김태희(Tae-Hee Kim),손아름(A-Reum Son),유송현(Song-Hyeon Yoo),김유린(Yu-Rin Kim) 한국구강보건과학회 2021 한국구강보건과학회지 Vol.9 No.1

Objectives: This study is intended to be used as basic data to enhance the image of dental hygienists by confirming the effect of the importance of dental hygienists on the image of dental hygienists. Methods: Hierarchical regression analysis was performed to determine the effect of dental hygienist importance and dental visit intervals on the dental hygienist image step by step. Results: As factors influencing the dental hygienist image, the younger, the more office workers, the shorter the dental visit interval. the higher the importance of the dental hygienist, the more significant the influence on the dental hygienist image was confirmed. Conclusions: In order to establish a professional image of dental hygienists, we expect continuous publicity that can increase the importance of dental hygienists and an increase in occupational ethics awareness that can increase the professional value of dental hygienists themselves.

Knockdown of acetylcholinesterase 1 increased the motility of honey bee worke

Sang Hyeon Kim,Kyung Mun Kim,Kil Won Kim,Si Hyeok Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

Acetylcholinesterase 1 (AmAChE1) has low catalytic activity and is abundantly expressed in both neuronal and non-neuronal tissues. In previous experiments, we observed that AmAChE1 is rarely expressed in summer while highly expressed in winter. Through additional experiments, the expression of AmAChE1 was suggested to be associated with brood rearing status. Under the assumption that abnormal suppression of brood rearing activity may result in stressful condition in honey bee social community, it was further suggested that AmAChE1 is likely involved in stress management particularly during winter. We hypothesized that the increased docility usually observed in overwintering bees is likely an outcome of stress management in colony, which is mediated by AmAChE1 expression. To verify this, worker bees expressing abundant AmAChE1 were collected in early winter and injected with Amace1 dsRNA to knockdown Amace1. Then, the behavioral activity of the bees was investigated using the EthoVison video tracking system. Honey bees injected with Amace1 dsRNA showed significantly increased motility, which was strongly correlated with the suppressed expression level of AmAChE1 in the abdomen. No apparent reduced expression of AmAChE1 in the head was observed perhaps due to the limited efficacy of RNA interference in the blood-brain-barrier. Our finding suggests that behavioral activity can be regulated, at least, by AmAChE1 expression level in non-neuronal tissue (i.e., fatbody) perhaps via metabolic alteration.

Expression level of acetylcholinesterase 1 is associated with honey bee brooding status

Young Ho Kim,Ju Hyeon Kim,Kyungmun Kim,Si Hyeock Lee 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

The acetylcholinesterase 1 (AmAChE1) of the honey bee is known to be abundantly expressed both in the central and peripheral nervous systems. AmAChE1 exists mostly in the soluble form with little catalytic activity and has non-neuronal functions. Our preliminary observation showed that AmAChE1 expression fluctuated between the forages and nurses. A more systematic expression profiling of AmAChE1 over a year cycle on a monthly basis revealed that AmAChE1 was predominantly expressed during the winter months with being moderately expressed during the rainy summer time. However, no significant difference in AmAChE1 expression was noticed between the nurse and forager workers. Interestingly, AmAChE1 expression was inhibited when bees were allowed for brooding by placing overwintering bee hives in strawberry green houses with the supplement of pollen diets whereas it was resumed when the bee hives were removed from the green houses, thereby suppressed brooding. To confirm whether brooding status is a main determining factor for the suppression of AmAChE1 expression, active bee hives were placed in a screen tent, thereby hindering foraging, until brooding was completely suppressed, and then allowed to restore brooding by removing the screen. The AmAChE1 expression in the head was up-regulated when brooding was suppressed whereas its expression was down-regulated when brooding was resumed. These finding demonstrates that AmAChE1 expression in the central nervous system (i.e., head) is related with brooding status of honey bee. To understand the connection between the AmAChE1 expression and other pathways related with brooding, currently in progress are the analyses of head transcriptomes of honey bee workers with or without their brooding suppressed.

Comparative Proteome Analysis of Honeybee Workers with or without Expressing Acetylcholinesterase 1

Young Ho Kim,Ju Hyeon Kim,Kyung Mun Kim,Si Hyeock Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

Recently, the expression of acetylcholinesterase1 (AChE1) in honeybee worker has been found to be seasonally fluctuated. Seasonal investigation on the AChE1 expression profiles revealed that it is abundantly expressed in January but its expression was completely abolished in February in both head and abdomen. In an attempt to predict the physiological function of seasonally expressed AChE1, proteomic analysis of honeybee worker was conducted using the samples collected in January and February. Total protein samples separately extracted from the head and abdomen of honeybee forager were compared by 2-D electrophoresis (2-DE). More than 2-fold differences in expression patterns between the two different samples were observed in 50 and 85 protein spots in the head and abdomen, respectively. Among them, 20 protein spots showing >17-fold differences in expression between the two different samples of the head were identified by mass spectrometry. Most of the proteins were identified to be the major royal jelly protein (MRJP) families (e.g., MRJP, MRJP2 and MRJP3), which are known to be expressed in nurse bees during brooding season, and their expression was significantly higher in January than in February. This result was unexpected because brooding usually began in the study site apiary during February and the worker bees used for analysis were assumed to be foragers (old workers). Thus, current findings suggest, though speculative, that the workers collected in January may function as nurses despite their old ages in January or that MRJPs may have other not-yet-characterized functions, which is apart from the conventionally known roles. Finally, possible association of MRJPs with AChE1 was discussed.

Effects of Wearable Powered Exoskeletal Training on Functional Mobility, Physiological Health and Quality of Life in Non-ambulatory Spinal Cord Injury Patients

Kim Hyeon Seong,Park Jae Hyeon,Lee Ho Seok,Lee Jae Young,Jung Ji Won,Park Si-Bog,Hyun Dong Jin,Park Sangin,Yoon JuYoung,Lim Hyunseop,Choi Yun Young,Kim Mi Jung 대한의학회 2021 Journal of Korean medical science Vol.36 No.12

Background: Spinal cord injury (SCI) is a serious clinical condition that impacts a patient's physical, psychological, and socio-economic status. The aim of this pilot study was to evaluate the effects of training with a newly developed powered wearable exoskeleton (Hyundai Medical Exoskeleton [H-MEX]) on functional mobility, physiological health, and quality of life in non-ambulatory SCI patients. Methods: Participants received 60 minutes of walking training with a powered exoskeleton 3 times per week for 10 weeks (total 30 sessions). The 6-minute walking test (6MWT) and timed-up-and-go test (TUGT) were performed to assess ambulatory function. The physiological outcomes of interest after exoskeleton-assisted walking training were spasticity, pulmonary function, bone mineral density, colon transit time, and serum inflammatory markers. Effects of walking training on subjective outcomes were estimated by the Korean version of the Falls Efficacy Scale—International and the 36-Item Short-Form Health Survey version 2. Results: Ten participants finished 30 sessions of training and could ambulate independently. No severe adverse events were reported during the study. After training, the mean distance walked in the 6MWT (49.13 m) was significantly enhanced compared with baseline (20.65 m). The results of the TUGT also indicated a statistically significant improvement in the times required to stand up, walk 3 m and sit down. Although not statistically significant, clinically meaningful changes in some secondary physiological outcomes and/or quality of life were reported in some participants. Conclusion: In conclusion, this study demonstrated that the newly developed wearable exoskeleton, H-MEX is safe and feasible for non-ambulatory SCI patients, and may have potential to improve quality of life of patients by assisting bipedal ambulation. These results suggest that the H-MEX can be considered a beneficial device for chronic non-ambulatory SCI patients.

A microfluidic device with 3-d hydrogel villi scaffold to simulate intestinal absorption.

Kim, Si Hyeon,Lee, Jung Woo,Choi, Inwook,Kim, Young-Chan,Lee, Jong Bum,Sung, Jong Hwan American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.11

<P>The absorption of drugs via oral route is a subject of a great interest in drug development process. The current in vitro method for measuring the kinetics of drug absorption relies on 2-D monolayer culture of Caco-2 cells on a porous membrane, but physiologically unrealistic environment provided by this method often results in inaccurate drug absorption kinetics. Here we report a novel microfluidic system which better mimics the physiological environment of the human small intestine. Three dimensional geometries of villi of the small intestine were reproduced via novel hydrogel microfabrication technique, and the fluid flow in the apical and basolateral sides of intestinal tract was reproduced with a two-layer microfluidic device. A wide range of flow rates was achieved by using gravity-induced flow, potentially facilitating easier high-throughput implementation. The kinetics of diffusion process through the 3-D villi scaffold in the microfluidic device was measured and mathematically modeled. When combined with intestinal cell culture model, this novel 3-D microfluidic system can serve as an in vitro platform that better mimics the in vivo environment.</P>