SPRY4-IT1 promotes survival of colorectal cancer cells through regulating PDK1-mediated glycolysis

Liu Shengyuan,Huang Feng,Ye Qing,Li Yangming,Chen Jinhu,Huang Hong 한국통합생물학회 2020 Animal cells and systems Vol.24 No.4

Colorectal cancer (CRC) becomes the third leading cause of cancer-related deaths worldwide recently. The prognosis of CRC is still poor in decades, and targeted therapy is still a potential effective treatment. Long non-coding RNAs (lncRNAs) could regulate series of cellular functions and developmental processes. LncRNA-SPRY4-IT1 (GenBank ID AK024556) is derived from an intron of the SPRY4 gene, which was highly expressed in melanoma cells and affected the progression of multiple types of cancers. However, the mechanism of SPRY4-IT1 in CRC progression remains unclear. Herein, we found the high level of SPRY4-IT1 in human colorectal cancer (CRC) tissues and cells, and correlated with patients’ prognosis. We further noticed that SPRY4-IT1 regulated CRC cell growth and glycolysis, and promoting PDK1 expression. Our data further confirmed that SPRY4-IT1 regulated CRC progression targeting PDK1. We therefore thought SPRY4-IT1 could serve as a promising molecular target for the treatment of CRC.

Comparative Patterns of Physiological Responses to Cold Resistance of Zanthoxylum bungeanum Maxim

Shengyuan Liu,Xiumei Liu,Xinying Han,Huatian Wang,Ping Xu,Chunmei Zhang 한국원예학회 2023 원예과학기술지 Vol.41 No.5

Aerodynamic modeling for streamlined box girders using nonlinear differential equations and validation in actively generated turbulence

Lin Zhao,Shengyuan Liu,Junfeng Yan,Yao-Jun Ge 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.33 No.1

In classical buffeting analysis theory, aerodynamic forces are usually expressed by a linear quasi-steady formula, and they are improved by aerodynamic admittances suitable for streamlined bridge girders. Recent studies have shown that admittances change obviously with incoming flow characteristics and aerodynamic nonlinearity, such as the frequency multiplication phenomenon, and motion-induced amplitude-related aerodynamic effects cannot be ignored in some cases. To address these problems, a nonlinear condensed subsystem equation (NCSE) suitable for wind-induced aerodynamic force modeling is established in the time domain. It characterizes aerodynamic nonlinearity with series of nonlinear differential equations and data-driven parameters. The proposed framework can be used for complex aerodynamic re-illustration related to the strong nonlinearity of streamlined box girders. To validate the precision and feasibility of the framework, sectional model experiments performed on a streamlined box girder were carried out in an active turbulence generated wind tunnel in which an adjustable array of multiple fans was assisted by actively controlled vibrating wings for a 2D turbulence condition. The case study shows that the NCSE model can be used to predict nonlinear aerodynamic forces in the time and frequency domains, even under complex stochastic flow conditions. The proposed method provides an alternative way to predict possible aerodynamics based on the condition of incoming flow with sufficient accuracy, and it can illustrate multifrequency components of aerodynamic forces.

Sox12 Is a Cancer Stem-Like Cell Marker in Hepatocellular Carcinoma

Zou, Song,Wang, Chen,Liu, Jiansheng,Wang, Qun,Zhang, Dongdong,Zhu, Shengnan,Xu, Shengyuan,Kang, Mafei,He, Shaozhong Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.11

Recent studies on molecular carcinogenesis suggest that the chemo-resistance of some cancers is largely due to presence of cancer stem cells (CSCs), which affect the chemotherapy outcome for hepatocellular carcinoma (HCC). However, currently no consensus on a CSC phenotype in HCC has been obtained. Here, we examined Sox12 as a novel CSC marker in HCC. Sox12+ versus Sox12- cells were purified from HCC cell lines. The Sox12+ cells were compared with Sox12- HCC cells for tumor sphere formation, chemo-resistance, tumor formation after serial adoptive transplantations in nude mice, and the frequency of developing distal metastasis. We found that compared to Sox12- HCC cells, Sox12+ HCC cells generated significantly more tumor spheres in culture, were more chemo-resistant to cisplatin, were detected in circulation more frequently, and formed distal tumor more frequently. Moreover, Sox12 appeared to functionally contribute to the stemness of HCC cells. Thus, we conclude that Sox12 may be a novel marker for enriching CSCs in HCC.

Sox12 Is a Cancer Stem-Like Cell Marker in Hepatocellular Carcinoma

Song Zou,Chen Wang,Jiansheng Liu,Qun Wang,Dongdong Zhang,Shengnan Zhu,Shengyuan Xu,Mafei Kang,Shaozhong He 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.11

Recent studies on molecular carcinogenesis suggest that the chemo-resistance of some cancers is largely due to presence of cancer stem cells (CSCs), which affect the chemotherapy outcome for hepatocellular carcinoma (HCC). However, currently no consensus on a CSC phenotype in HCC has been obtained. Here, we examined Sox12 as a novel CSC marker in HCC. Sox12+ versus Sox12- cells were purified from HCC cell lines. The Sox12+ cells were compared with Sox12- HCC cells for tumor sphere formation, chemo-resistance, tumor formation after serial adoptive transplantations in nude mice, and the frequency of developing distal metastasis. We found that compared to Sox12- HCC cells, Sox12+ HCC cells generated significantly more tumor spheres in culture, were more chemo-resistant to cisplatin, were detected in circulation more frequently, and formed distal tumor more frequently. Moreover, Sox12 ap-peared to functionally contribute to the stemness of HCC cells. Thus, we conclude that Sox12 may be a novel marker for enriching CSCs in HCC.

Magnetic Resonance Image Texture Analysis of the Periaqueductal Gray Matter in Episodic Migraine Patients without T2-Visible Lesions

Zhiye Chen,Xiaoyan Chen,Mengqi Liu,Shuangfeng Liu,Shengyuan Yu,Lin Ma 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.1

Objective: The periaqueductal gray matter (PAG), a small midbrain structure, presents dysfunction in migraine. However, the precise neurological mechanism is still not well understood. Herein, the aim of this study was to investigate the texture characteristics of altered PAG in episodic migraine (EM) patients based on high resolution brain structural magnetic resonance (MR) images. Materials and Methods: The brain structural MR images were obtained from 18 normal controls (NC), 18 EM patients and 16 chronic migraine (CM) patients using a 3T MR system. A PAG template was created using the International Consortium Brain Mapping 152 gray matter model, and the individual PAG segment was developed by applying the deformation field from the structural image segment to the PAG template. A grey level co-occurrence matrix was used to calculate the texture parameters including the angular second moment (ASM), contrast, correlation, inverse difference moment (IDM) and entropy. Results: There was a significant difference for ASM, IDM and entropy in the EM group (998.629 ± 0.162 x 10-3, 999.311 ± 0.073 x 10-3, 916.354 ± 0.947 x 10-5) compared to that found in the NC group (998.760 ± 0.110 x 10-3, 999.358 ± 0.037 x 10-3 and 841.198 ± 0.575 x 10-5) (p < 0.05). The entropy was significantly lower among the patients with CM (864.116 ± 0.571 x 10-5) than that found among patients with EM (p < 0.05). The area under the receiver operating characteristic curve was 0.776 and 0.750 for ASM and entropy in the distinction of the EM from NC groups, respectively. ASM was negatively related to disease duration (DD) and the Migraine Disability Assessment Scale (MIDAS) scores in the EM group, and entropy was positively related to DD and MIDAS in the EM group (p < 0.05). Conclusion: The present study identified altered MR image texture characteristics of the PAG in EM. The identified texture characteristics could be considered as imaging biomarkers for EM.

Simple denoising method for novel speckle-shifting ghost imaging with connected-region labeling

Sheng Yuan,Xuemei Liu,Pibin Bing 한국광학회 2019 Current Optics and Photonics Vol.3 No.3

A novel speckle-shifting ghost imaging (SSGI) technique is proposed in this paper. This method can effectively extract the edge of an unknown object without achieving its clear ghost image beforehand. However, owing to the imaging mechanism of SSGI, the imaging result generally contains serious noise. To solve the problem, we further propose a simple and effective method to remove noise from the speckle-shifting ghost image with a connected-region labeling (CRL) algorithm. In this method, two ghost images of an object are first generated according to SSGI. A threshold and the CRL are then used to remove noise from the imaging results in turn. This method can retrieve a high-quality image of an object with fewer measurements. Numerical simulations are carried out to verify the feasibility and effectiveness.

Optical Encryption Scheme with Multiple Users Based on Computational Ghost Imaging and Orthogonal Modulation

Sheng Yuan,Xuemei Liu,Xin Zhou,Zhongyang Li 한국광학회 2016 Current Optics and Photonics Vol.20 No.4

For the application of multiusers, the arrangement and distribution of the keys is a much concerningproblem in a cryptosystem. In this paper, we propose an optical encryption scheme with multiple usersbased on computational ghost imaging (CGI) and orthogonal modulation. The CGI encrypts the secret imageinto an intensity vector rather than a complex-valued matrix. This will bring convenience for post-processingand transmission of the ciphertext. The orthogonal vectors are taken as the address codes to distinguishusers and avoid cross-talk. Only the decryption key and the address code owned by an authorized userare matched, the secret image belonging to him/her could be extracted from the ciphertext. Therefore, thereare two security levels in the encryption scheme. The feasibility and property are verified by numericalsimulations.

Remarkably low genetic diversity and shallow population structure detected of the spiny eel (Sinobdella sinensis) in Eastern China

Sheng Yuan,Meijian Liu,Ziming Zhao 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background The spiny eel (Sinobdella sinensis) is a small subtropical fish endemic to China, Vietnam, and Laos. It hasdisappeared in many rivers and lakes due to anthropogenic stressors. Objective The aim of this study was to investigate the genetic diversity and population structure and to provide pertinentinformation of the evolutionary history and conservation of S. sinensis. Methods Mitochondrial DNA (mtDNA) cytochrome c oxidase I (COI) sequences of 144 individuals from five lakes in theJiangsu Province of Eastern China were sequenced. Results A total of 17 haplotypes were defined by 20 variable nucleotide sites. Remarkably low haplotype and nucleotidediversity was observed in all sampled populations. The AMOVA analyses revealed that 96.44% of the genetic variationoccurred within the populations. Significant genetic differentiation was detected among populations (P < 0.05), but nolarge-scale regional differences were detected. Analysis of neutral evolution and mismatch distribution suggests populationexpansion. Low genetic diversity and shallow population structure based on COI sequences were also confirmed. Conclusions The wild resource of S. sinensis in Eastern China has sharply declined. Low genetic diversity and shallowpopulation structure based on COI sequences were confirmed. Fishing management and resource conservation of this speciesshould be taken urgently.