CircKLHL2 mitigates septic lung injury via circKLHL2-miR-338-3p-ATF6 ceRNA pathway

Zhang Chunmei,Wu Ruoran,Zhao Zhongyan 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Background Circular RNAs (circRNAs) are newly identified therapeutic targets for sepsis. CircRNA mmu_circ_0001679 dysregulation is underlying the pathogenesis and treatment of sepsis-induced acute lung injury (ALI). Here, the expression and role of its human homologoue circRNA-Kelch-like family member 2 (circKLHL2; hsa_circ_0071374) were explored in lipopolysaccharide (LPS)-induced human ALI. Objective Expression levels of circKLHL2, microRNA (miR)-338-3p and activating transcription factor 6 (ATF6) were detected by quantitative PCR and western blotting, and dual-luciferase reporter assay confirmed target relationships among them. Cell injury was measured using cell-counting kit-8, reactive oxygen species (ROS)/malondialdehyde (MDA)/superoxide dismutase (SOD) assays, Annexin V-fluorescein isothiocyanate apoptosis assay, western blotting and cysteine-requiring aspartate proteases (caspases) activity assays. Result The expression of circKLHL2 and ATF6 was upregulated in sepsis-damaged human lung tissues and LPS-stressed human bronchial epithelial cells (16HBE), with a concomitant decrease of miR-338-3p. RNA interfering of circKLHL2 improved cell viability of LPS-challenged 16HBE cells, and suppressed apoptosis, oxidative stress and endoplasmic reticulum (ER) stress, as evidenced by the increased bcl-2 level and SOD activity, and the decreased apoptosis rate, caspase3/9 activity and levels of bcl-2-associated X protein, ROS, MDA, ATF6 and C/EBP-homologous protein. Moreover, circKLHL2 knockdown exerted similar results to ER stress inhibitor 4-phenyl butyric acid in LPS injury. In addition, circKLHL2 knockdown reversed LPS-induced mitochondrial dysfunction. ATF6 and circKLHL2 were competing endogenous RNAs of miR-338-3p, and either exhausting miR-338-3p or restoring ATF6 could counteract circKLHL2 knockdown-mediated roles in LPS injuries. Conclusion Depleting circKLHL2 suppressed LPS-induced ALI through suppressing mitochondrial dysfunction and cytotoxicity and oxidative and ER stresses-induced apoptosis via circKLHL2-miR-338-3p/ATF6 axis.

Graph Theory-based Approach for Partial Topology Identification of Stochastic Multi-group Models With Multiple Dispersal

Chunmei Zhang,Dan Xia,Huiling Chen,Guiling Chen 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.9

This paper is mainly concerned with partial topology identification of stochastic multi-group models with multiple dispersals (SMGMMD) by adaptive pinning control. By using a graph-theoretic approach and adaptive synchronization techniques, some sufficient criteria for partial topology identification of SMGMMD with time delay are obtained. Meanwhile, the partial topological structures of SMGMMD without time delay and the whole topological structures of SMGMMD can also be identified successfully. Finally, coupled Lorenz systems with time delay are identified to demonstrate the feasibility and effectiveness of theoretical results.

Iron-manganese-magnesium mixed oxides catalysts for selective catalytic reduction of NOx with NH3

Kang Zhang,Liting Xu,Shengli Niu,Chunmei Lu,Dong Wang,Qi Zhang,Jing Li 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.6

SCR activity at low temperature over iron oxide catalyst was prominently optimized by adding manganese and magnesium. Fe0.7Mn0.15Mg0.15Oz(n(Mn)/[n(Fe)+n(Mn)+n(Mg)])=0.15 and n(Mg)/[n(Fe)+n(Mn)+n(Mg)]=0.15) presented better performance in the low temperature SCR and NOx conversion of 90% could be achieved over 125 oC. Meanwhile, part of manganese and magnesium oxides were highly dispersed on the catalyst surface in an amorphous phase to react with iron oxide to form solid solution. Manganese and magnesium dopants could optimize the pore structure and distribution of γ-Fe2O3 to enhance the surface area and pore volume. Moreover, O2 participated in SCR reaction at a faster rate than NH3. In addition, the effect of SO2 was proved to be irreversible, whereas the inhibition of H2O could be rapidly removed after its removal.

EXISTENCE OF POSITIVE SOLUTIONS FOR SINGULAR IMPULSIVE DIFFERENTIAL EQUATIONS WITH INTEGRAL BOUNDARY CONDITIONS

Chunmei Miao,Wei-Gao Ge,Zhaojun Zhang 한국수학교육학회 2014 純粹 및 應用數學 Vol.21 No.3

In this paper, we study the existence of positive solutions for singularimpulsive differential equations with integral boundary conditions "( )+ ( ) ( , ( ), '( ))=0, ∊ ',∆ ( )= ( ( ), '( )), =1,2,…, ,∆ '( )=- ( ( ), '( )), =1,2,…, , (0)=∫10 ( ) ( ) , '(1)=0,where the nonlinearity f(t, u, v) may be singular at v = 0. The proof is based onthe theory of Leray-Schauder degree, together with a truncation technique. Somerecent results in the literature are generalized and improved.

Smaller Body Size, Early Postnatal Lethality, and Cortical Extracellular Matrix-Related Gene Expression Changes of <i>Cyfip2</i> -Null Embryonic Mice

Zhang, Yinhua,Kang, Hyojin,Lee, Yeunkum,Kim, Yoonhee,Lee, Bokyoung,Kim, Jin Yong,Jin, Chunmei,Kim, Shinhyun,Kim, Hyun,Han, Kihoon Frontiers Media S.A. 2018 Frontiers in molecular neuroscience Vol.11 No.-

<P>Cytoplasmic FMR1-interacting protein 2 (CYFIP2) is a key component of the WAVE regulatory complex (WRC) which regulates actin polymerization and branching in diverse cellular compartments. Recent whole exome sequencing studies identified <I>de novo</I> hotspot variants in <I>CYFIP2</I> from patients with early-onset epileptic encephalopathy and microcephaly, suggesting that CYFIP2 may have some functions in embryonic brain development. Although perinatal lethality of <I>Cyfip2</I>-null (<I>Cyfip2</I><SUP>−/−</SUP>) mice was reported, the exact developmental time point and cause of lethality, and whether <I>Cyfip2</I><SUP>−/−</SUP> embryonic mice have brain abnormalities remain unknown. We found that endogenous <I>Cyfip2</I> is mainly expressed in the brain, spinal cord, and thymus of mice at late embryonic stages. <I>Cyfip2</I><SUP>−/−</SUP> embryos did not show lethality at embryonic day 18.5 (E18.5), but their body size was smaller than that of wild-type (WT) or <I>Cyfip2</I><SUP>+/−</SUP> littermates. Meanwhile, at postnatal day 0, all identified <I>Cyfip2</I><SUP>−/−</SUP> mice were found dead, suggesting early postnatal lethality of the mice. Nevertheless, the brain size and cortical cytoarchitecture were comparable among WT, <I>Cyfip2</I><SUP>+/−</SUP>, and <I>Cyfip2</I><SUP>−/−</SUP> mice at E18.5. Using RNA-sequencing analyses, we identified 98 and 72 differentially expressed genes (DEGs) from the E18.5 cortex of <I>Cyfip2</I><SUP>+/−</SUP> and <I>Cyfip2</I><SUP>−/−</SUP> mice, respectively. Further bioinformatic analyses suggested that extracellular matrix (ECM)-related gene expression changes in <I>Cyfip2</I><SUP>−/−</SUP> embryonic cortex. Together, our results suggest that CYFIP2 is critical for embryonic body growth and for early postnatal survival, and that loss of its expression leads to ECM-related gene expression changes in the embryonic cortex without severe gross morphological defects.</P>

POSITIVE SOLUTION FOR SYSTEMS OF NONLINEAR SINGULAR BOUNDARY VALUE PROBLEMS ON TIME SCALES

Chunmei Miao,Dehong Ji,Junfang Zhao,Weigao Ge,Jiani Zhang 한국수학교육학회 2009 純粹 및 應用數學 Vol.16 No.4

In this paper, we deal with the following system of nonlinear singular boundary value problems(BVPs) on time scale T [수식] where [수식] and [수식] may be singular at t = a, y = 0, and g(t, x) may be singular at t = a. The arguments are based upon a fixed-point theorem for mappings that are decreasing with respect to a cone. We also obtain the analogous existence results for the related nonlinear systems [수식], and [수식] satisfying similar boundary conditions.

Multi-armed Bandit Online Learning Based on POMDP in Cognitive Radio

Juan Zhang,Hesong-Jiang,Hong Jiang,Chunmei Chen 보안공학연구지원센터 2014 International Journal of Smart Home Vol.8 No.3

Metallogenesis and hydrothermal evolution of the Saibo copper deposit in the western Tianshan: evidence from fluid inclusions, H-O-S isotopes, and Re-Os geochronology

Bowen Zhang,Xiaofei Tang,Chuan Chen,Xiaoping Gong,Chunmei Su 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.1

The Saibo copper polymetallic deposit, located in the West Tianshan Sailimu-Sitaihaiquan copper lead and zinc ore belt, is a new breakthrough in medium-large copper mine. Through the thin section authentication and the thermometric analysis of fluid inclusion, it is concluded that skarnization was developed in this deposit, the mineralization stage can be divided into retrograde skarn stage (S1), quartz-sulfide stage (S2) and quartz-carbonate stage (S3). Fluid inclusions (FIs) were distinguished as liquid-rich aqueous FIs (LV-type), vapor-rich aqueous FIs (VL-type), and NaCl daughter mineral-bearing three phase FIs (S-type). The mineralization fluids indicate that the initial stage (S1 stage) is of high temperature and high salinity, with a handful of metallicmatters. During the main metallogenic stage (S2 stage), the meteoric water is mixed, the temperature and salinity gradually decrease, the fluid is distinctly boiling, and a large amount of metallic matters are precipitated, and then to the S3 stage, when the temperature and salinity decrease along with the carbonation. Chalcopyrite Re-Os dating yielded a mineralization age of 379.2 ± 7.7 Ma, which corresponds well with the zircon U-Pb age (385.9 ± 1.3 Ma) of the granite porphyry. The C-H-O isotopes indicate that the ore-forming fluid was dominated by initial mixed magmatic water in the early stage and by meteoric water in the late stage, and C is derived from limestone strata metasomatized by magmatic fluids. The S and Pb isotopes indicate that the ore-forming materials are derived from the mixed crust-mantle source, of which S is almost entirely derived from the mantle, while Pb is mainly derived from the crustal material of the orogenic belt. On the whole, the Saibo copper deposit is a typical calcium skarn type deposit, which formed in the shallower setting at active continental margin in the Late Devonian period.

Apoptosis and autophagy of muscle cell during pork postmortem aging

Li Chunmei,Yin Xialian,Xue Panpan,Wang Feng,Song Ruilong,Song Qi,Su Jiamin,Zhang Haifeng 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2

Objective: Pork is an important source of animal protein in many countries. Subtle physiochemical changes occur during pork postmortem aging. The changes of apoptosis and autophagy in pork at 6 h to 72 h after slaughter were studied to provide evidence for pork quality. Methods: In this article, morphological changes of postmortem pork was observed through Hematoxylin-eosin staining, apoptotic nuclei were observed by TdT-mediated dUTP nick end labeling assay, protein related to apoptosis and autophagy expressions were tested by western blot and LC3 level were expressed according to immunofluorescence assay. Results: In this study, we found the occurrence of apoptosis in postmortem pork, and the process was characterized by nucleus condensation and fragmentation, formation of apoptotic bodies, increase in apoptosis-related Bax/Bcl-2 levels, and activation of caspases. Autophagy reached its peak between 24 and 48 h after slaughter, accompanied by the formation of autophagosomes on the cell membrane and expression of autophagy-related proteins beclin-1, P62, LC3-I, LC3-II, and ATG5. Conclusion: Obvious apoptosis was observed at 12 h and autophagy reached its peak at 48 h. The present work provides the evidence for the occurrence of apoptosis and autophagy during postmortem aging of pork. In conclusion, the apoptosis and autophagy of muscle cells discovered in this study have important implications for pork in the meat industry.

Integrative Brain Transcriptome Analysis Reveals Region-Specific and Broad Molecular Changes in <i>Shank3</i> -Overexpressing Mice

Jin, Chunmei,Kang, Hyojin,Ryu, Jae Ryun,Kim, Shinhyun,Zhang, Yinhua,Lee, Yeunkum,Kim, Yoonhee,Han, Kihoon Frontiers Media S.A. 2018 Frontiers in molecular neuroscience Vol.11 No.-

<P>Variants of the SH3 and multiple ankyrin repeat domain 3 (<I>SHANK3</I>) gene, encoding excitatory postsynaptic core scaffolding proteins, are causally associated with numerous neurodevelopmental and neuropsychiatric disorders, including autism spectrum disorder (ASD), bipolar disorder, intellectual disability, and schizophrenia (SCZ). Although detailed synaptic changes of various <I>Shank3</I> mutant mice have been well characterized, broader downstream molecular changes, including direct and indirect changes, remain largely unknown. To address this issue, we performed a transcriptome analysis of the medial prefrontal cortex (mPFC) of adult <I>Shank3</I>-overexpressing transgenic (TG) mice, using an RNA-sequencing approach. We also re-analyzed previously reported RNA-sequencing results of the striatum of adult <I>Shank3</I> TG mice and of the prefrontal cortex of juvenile <I>Shank3<SUP>+/</SUP></I><SUP>Δ</SUP><I><SUP>C</SUP></I> mice with a 50–70% reduction of Shank3 proteins. We found that several myelin-related genes were significantly downregulated specifically in the mPFC, but not in the striatum or hippocampus, of adult <I>Shank3</I> TG mice by comparing the differentially expressed genes (DEGs) of the analyses side by side. Moreover, we also found nine common DEGs between the mPFC and striatum of <I>Shank3</I> TG mice, among which we further characterized ASD- and SCZ-associated G protein-coupled receptor 85 (<I>Gpr85</I>), encoding an orphan <I>Gpr</I> interacting with PSD-95. Unlike the mPFC-specific decrease of myelin-related genes, we found that the mRNA levels of <I>Gpr85</I> increased in multiple brain regions of adult <I>Shank3</I> TG mice, whereas the mRNA levels of its family members, <I>Gpr27</I> and <I>Gpr173</I>, decreased in the cortex and striatum. Intriguingly, in cultured neurons, the mRNA levels of <I>Gpr27</I>, <I>Gpr85</I>, and <I>Gpr173</I> were modulated by the neuronal activity. Furthermore, exogenously expressed GPR85 was co-localized with PSD-95 and Shank3 in cultured neurons and negatively regulated the number of excitatory synapses, suggesting its potential role in homeostatic regulation of excitatory synapses in <I>Shank3</I> TG neurons. Finally, we performed a gene set enrichment analysis of the RNA-sequencing results, which suggested that Shank3 could affect the directional expression pattern of numerous ribosome-related genes in a dosage-dependent manner. To sum up, these results reveal previously unidentified brain region-specific and broad molecular changes in <I>Shank3</I>-overexpressing mice, further elucidating the complexity of the molecular pathophysiology of <I>SHANK3</I>-associated brain disorders.</P>