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Lee, Jong Lyul,Roh, Seon Ae,Kim, Chan Wook,Kwon, Yi Hong,Ha, Ye Jin,Kim, Seon-Kyu,Kim, Seon-Young,Cho, Dong-Hyung,Kim, Yong Sung,Kim, Jin Cheon Baishideng Publishing Group Inc 2019 WORLD JOURNAL OF GASTROENTEROLOGY Vol.25 No.11
<P><B>BACKGROUND</B></P><P>Genomic profiling of tumors has contributed to the understanding of colorectal cancer (CRC), facilitating diagnosis, prognosis and selection of treatments, including targeted regimens. A report suggested that a 19-gene-based risk classifier (TCA19) was a prognostic tool for patients with stage III CRC. The survival outcomes in patients with stage IV CRC are still poor and appropriate selection of targeted therapies and immunotherapies is challenging.</P><P><B>AIM</B></P><P>To assess clinical implication of TCA19 in patients with stage IV CRC, and to identify TCA19 with involvement in immune-oncology.</P><P><B>METHODS</B></P><P>A retrospective review of the medical records of 60 patients with stage IV CRC was conducted, assessing clinicopathological variables and progression-free survival (PFS). TCA19 gene expression was determined by quantitative polymerase chain reaction (qPCR) in matched normal and tumor tissues taken from the study cohort. Expression of potential immune-oncology regulatory proteins and targets was examined by immunohistochemistry (IHC), western blot, immunofluorescence staining in tissues from a validation cohort of 10 patients, and in CRC cell lines co-cultured with monocyte <I>in vitro</I>.</P><P><B>RESULTS</B></P><P>In the patients with TCA19 score higher than the median, the PFS rates of eight patients who received the targeted regimens were significantly higher than the PFS rates of four patients who received 5-fluorouracil-based regimen (<I>P</I> = 0.041). In multivariate analysis, expression of signaling lymphocytic activation molecule family, member 7 (SLAMF7) and triggering receptor expressed on myeloid cells 1 (TREM1) was associated with PFS in the 60-patient cohort. After checking another 10 validate set, the expression of the IHC, the level of real-time qPCR, and the level of western blot were lower for SLAMF7 and higher for TREM7 in primary and metastatic tumors than in normal tissues. In CRC cells expressing SLAMF7 that were co-cultured with a monocytic cell line, levels of CD 68 and CD 73 were significantly lower at day 5 of co-culture than at day 0.</P><P><B>CONCLUSION</B></P><P>The TCA19 score might be prognostic for target-regimen-specific PFS in stage IV CRC. Down-regulation of SLAMF7 and up-regulation of TREM1 occur in primary and metastatic tumor tissues.</P>
Seon-Kyu Kim,Seon-Young Kim,Chan Wook Kim,Seon Ae Roh,하예진,Jong Lyul Lee,Haejeong Heo,Dong-Hyung Cho,이주석,Yong Sung Kim,Jin Cheon Kim 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-
Approximately half of colorectal cancer (CRC) patients experience disease recurrence and metastasis, and theseindividuals frequently fail to respond to treatment due to their clinical and biological diversity. Here, we aimed toidentify a prognostic signature consisting of a small gene group for precisely predicting CRC heterogeneity. Weperformed transcriptomic profiling using RNA-seq data generated from the primary tissue samples of 130 CRCpatients. A prognostic index (PI) based on recurrence-associated genes was developed and validated in two largerindependent CRC patient cohorts (n = 795). The association between the PI and prognosis of CRC patients wasevaluated using Kaplan–Meier plots, log-rank tests, a Cox regression analysis and a RT-PCR analysis. Transcriptomicprofiling in 130 CRC patients identified two distinct subtypes associated with systemic recurrence. Pathwayenrichment and RT-PCR analyses revealed an eleven gene signature incorporated into the PI system, which was asignificant prognostic indicator of CRC. Multivariate and subset analyses showed that PI was an independent risk factor(HR = 1.812, 95% CI = 1.342–2.448, P < 0.001) with predictive value to identify low-risk stage II patients who respondedthe worst to adjuvant chemotherapy. Finally, a comparative analysis with previously reported Consensus MolecularSubgroup (CMS), high-risk patients classified by the PI revealed a distinct molecular property similar to CMS4,associated with a poor prognosis. This novel PI predictor based on an eleven gene signature likely represents asurrogate diagnostic tool for identifying high-risk CRC patients and for predicting the worst responding patients foradjuvant chemotherapy.
Seon, C R,Hong, J H,Jang, J,Lee, S H,Choe, W,Lee, H H,Cheon, M S,Pak, S,Lee, H G,Biel, W,Barnsley, R American Institute of Physics 2014 Review of scientific instruments Vol.85 No.11
<P>To optimize the design of ITER vacuum ultraviolet (VUV) spectrometer, a prototype VUV spectrometer was developed. The sensitivity calibration curve of the spectrometer was calculated from the mirror reflectivity, the grating efficiency, and the detector efficiency. The calibration curve was consistent with the calibration points derived in the experiment using the calibrated hollow cathode lamp. For the application of the prototype ITER VUV spectrometer, the prototype spectrometer was installed at KSTAR, and various impurity emission lines could be measured. By analyzing about 100 shots, strong positive correlation between the O VI and the C IV emission intensities could be found.</P>
천선희 ( Cheon Seon Hui ),김세규 ( Kim Se Gyu ),정현철 ( Jeong Hyeon Cheol ),장준 ( Jang Jun ),손희영 ( Son Hui Yeong ),김성규 ( Kim Seong Gyu ),이원영 ( Lee Won Yeong ),이두연 ( Lee Du Yeon ),이기범 ( Lee Gi Beom ) 대한내과학회 1992 대한내과학회지 Vol.42 No.4
연구배경 : Carcinoembryonic antigen은 oncodev-elopmental tissue glycoprotein으로 대장 및 직장암, 유방암, 페암 환자의 혈청에서 검출되어 진단 및 치료 경과의 관찰에 이용되고 있으며, Epidermal growth factor receptor는 상피세포의 세포막에 존재하는 수용체로써 EGF와 반응하여 상피세포의 분화와 증식을 조절하는 물질로 인체 세포 내에 존재하는 proto-on-cogene인 c-erb-B의 산물이며, 증가된 발현과 더불어 EGFR 또는 그와 관련된 gene의 비정상적인 발현이 상피세포암의 발현에 관여하리라 보고 있다. 방 법: 1986년 1월부터 1989년 12월까지 연세대학교 의과대학 세브란스 병원에서 원발성 폐암 진단 후 폐절제술을 시행 받고 생존 여부와 치료 성적으로 알고있는 원발성 비소세포 폐암 환자를 대상으로 CEA와 EGFR에 대한 면역세포화학적 염색을 시행하였다. 결과 : 1) 한국인 비소세포 폐암에서 CEA 발현율이 96%이었으며, EGFR발현율은 26%이었다. 2) 암세포 유형에 따른 EGFR 발현율은 편평상피암 30%, 선암 14%, 미분화암 50%이었으며, 대세포암은 4예 모두 음성 이었고, grading system 적용시 1 혹은 2 +로 발현 정도가 낮았다. 3) Grading system을 적용한 암세포 유형에 따른 CEA 발현율은 편평상피암 22%, 선암 93%, 미분화함 33%가 3 혹은 4+로, 선암의 CEA 발현율이 가장 높아TEk. 4) 대상환자의 평균 추적 기가은 2년(1~41개월)으로, 1년 생존율은 85%, 2년 생존율은 68%, 3년 생존율은 54%이었으며, EGFR 과 CEA의 발현 정도에 따른 생존율에는 차이가 없었다. 결론 : 한국인 비세포 폐암에서도 CEA와 EGFR이 발현함을 확인 하였으며, EGFR의 발현 유무가 폐암 생존율의 예후인자로 작용할 가능성 여부에 대하여서는 추후 연구가 필요할 것으로 생각된다. Background: Carcinoembryonic antigen (CEA) was defined as an endodermally derived tumor-associated antigen that was also present in fetal gastrointestinal tissues. This antigenic material has been demonstrated in the blood of a large number of patients with other neoplasms, including those arising in the lung. Epidermal Growth Factor (EGF) usually stimulate the growth and proliferation of a variety of cell types through binding to a specific cell surface receptor, a 170-KD glycoprotein, EGFR. A part of the EGFR has been found to be homologous to the product of the avian erythroblastosis virus oncogene, c-erb-B. Many squamous cell carcinoma cell lines show high levels of EGFR expression and the EGFR apparently plays an important role in the growth of squamous cell carcinoma. Methods: We have collected paraffin blocks of 100 patients with non-small cell lung cancer who were treated with surgical resection and assessed their CEA and EGFR expression with monoclonal antibodies against the CEA or EGFR by immunohistochemical stain (ABC method). Results: 1) The expression of CEA was 96% and the expression of EGFR was 26% in the Korean non-small cell lung cancer. 2) EGFR immunoreactivity was detected in 19 (30%) of the 63 epidermoid carcinomas, in 4 (14%) of the 27 adenocarcinomas and in 3 (50%) of the 6 undifferentiated carcinomas, while no EGFR immunoreactivity was found in large cell carcinomas. By using the grading system, the EGFR expression grades were low a s grade 1 or 2 +. 3) CEA immunoreactivity by using the grading system was 3 or 4 + in 14 (22%) of the 63 epidermoid carcinomas, in 25 (93%) of the 27 adenocarcinomas and in 2 (33%) of the 6 undifferentiated carcinomas. So the highest prevalence of intracellular CEA was seen in the adenocarcinomas, including the bronchoalveolar type. 4) The median follow-up duration was 2 years (1-41 months) and the 1-year survival rate was 85%, the 2-year survival rate 68% and the 3-year survival rate 54%. A correlation was not demonstrated between the expression rate of CEA or EGFR and the survival rate of lung cancer patients. Conclusions: CEA and EGFR expression in Korean non-small cell lung cancer were observed. But further studies need to be performed to determine whether EGFR expression can be prognostic of lung cancer or not.
이선영 ( Seon-young Lee ),박대천 ( Dae-cheon Park ),강대식 ( Dae Sik Kang ),이충호 ( Choong Ho Lee ) 한국농업기계학회 2020 한국농업기계학회 학술발표논문집 Vol.25 No.2
땅속 작물 수거기가 지면 위의 작물을 수거하기 위해서는 수거가 이루어지는 속도와 기계의 주행속도가 적절한 조화를 이루어야 한다. 수거기의 주행 변속은 전진 2단(0.20m/s, 0.61m/s), 후진 1단으로 설계되었으며 동력전달은 기어체인과 벨트풀리를 사용한다. 그림과 같이 수거기의 동력전달장치는 6개의 유니트로 구성되어 있으며 각 축을 연결하여 수거회전 부분과 컨베이어 부분으로 동력이 전달된다. 본 연구는 구간별 동력에 대해 설계를 진행하였다. 수식을 통해 구간별 평균속도와 최대, 최소속도를 정의하고 속도 변동률을 도출하였다. 전달힘에 따라 동력을 산출하고 수식으로 정리한 전달 동력은 2.3kw로 기계적 손실을 고려하여 2.5kw 이상의 동력을 사용하는 것이 좋을 것으로 판단된다. 도출된 결과값은 땅속 작물 수거기의 제작에 적용하였다. 결과를 토대로 하중에 따른 속도 분석을 수행하여 부하되는 하중에 대한 최적화 설계가 가능할 것으로 사료된다.
Stimulation of the Extracellular Matrix Production in Dermal Fibroblasts by Velvet Antler Extract
( Seok Seon Roh ),( Min Ho Lee ),( Yul Lye Hwang ),( Hyun Hee Song ),( Mu Hyun Jin ),( Sun Gyoo Park ),( Cheon Koo Lee ),( Chang Deok Kim ),( Tae Jin Yoon ),( Jeung Hoon Lee ) 대한피부과학회 2010 Annals of Dermatology Vol.22 No.2
Background: Fibroblasts produce many components of the extracellular matrix (ECM) and so they contribute to the maintenance of connective tissue integrity. Objective: The aim of this study is to evaluate the effect of velvet antler extract (VAE) on the ECM production of dermal fibroblasts cultured in vitro. Methods: Primary cultured human dermal fibroblasts were treated with VAE, and then the ECM production was determined by RT-PCR, ELISA and Western blot analysis. Furthermore, the change of gene expression according to VAE treatment was evaluated by cDNA microarray. Results: VAE accelerated the growth of fibroblasts in a dose-dependent manner. VAE increased the production of several ECM components, including type 1 collagen, fibronectin and elastin. In line with these results, the phosphorylations of p42/44 ERK and p38 mitogen-activated protein kinase were markedly increased by VAE, suggesting that the enhancement of ECM production may be linked to the activation of intracellular signaling cascades. VAE also significantly increased cell migration on an in vitro scratch wound test. In cDNA microarray, many genes related with connective tissue integrity were identified to be up-regulated by VAE. Conclusion: These results suggest that VAE has a potential to stimulate ECM production, and VAE may be applicable for maintaining the skin`s texture. (Ann Dermatol 22(2) 173~179, 2010)