Therapeutic Effects of Recombinant Human Epidermal Growth Factor (rhEGF) in a Murine Model of Concurrent Chemo- and Radiotherapy-Induced Oral Mucositis

RYU, Seung-Hee,KANG, Ki Mun,MOON, Soo Young,CHAI, Gyu Young,HONG, Joon Pio,CHO, Kyoung-Oh,KANG, Mun-Il,CHOI, Eun Kyung,LEE, Sang-wook Journal of Radiation Research Editorial Committee 2010 JOURNAL OF RADIATION RESEARCH Vol.51 No.5

<P>Concurrent chemotherapy with radiotherapy (CCRT) has been applied for the treatment of advanced stage of head and neck cancer patients. However CCRT is associated with several complications including mucositis, dermatitis, stomatitis, etc. This study was conducted to evaluate the therapeutic effect of systemically administrated recombinant human epidermal growth factor (rhEGF) in CCRT-induced oral mucositis in a mouse model. Oral mucositis was induced in male BALB/c mice through combination treatment with cisplatin (11 mg/kg, i.p.) and irradiation (17 Gy) of the head and neck area. rhEGF (1.0 mg/kg/day for consecutive 3 days) was administered systemically, and the therapeutic effect was determined by histological evaluation of the oral mucosa. To elucidate optimal dose of rhEGF on CCRT-induced mucositis, various concentrations (0.04–3 mg/kg) of rhEGF were injected for 3 days. Systemic rhEGF administration accelerated the recovery of body weight. Histologically, rhEGF-treated mice showed significantly increased epithelial cell layer thickness, basal cell number, and expression of Ki-67 compared to control mice. Most effective dose was 1 mg/kg among other doses tested. Systemic administration of 1 mg/kg of rhEGF reduces the severity of oral mucositis induced by CCRT in a mouse model, suggesting that rhEGF can be used for treating CCRT-induced mucositis during the cancer treatment.</P>

六味地黃湯이 生理活性指標와 淋巴球細胞數에 미치는 影響

金榮權,柳逢夏,朴東源,柳基遠 대한한방종양학회 1998 대한한방종양학회지 Vol.4 No.1

Yookmijihwangtang has been widely used oriental herb prescriptions, which is healing some discuss that come from insufficiency of innate essence and deficiency of kidey Ki. the meaning of healing discusses tonification of insufficient innate essence and insufficient kidney Ki can be regarded as reinforcement of wholely power of keeping homeostasis, that is correlated with immuno-responsibility which protects subject from outer antigen to keep normal vital condition. This study was aimed to investigate correlative effects of Yookmijihw angtang water abstract on the RBC, WBC, blood CD4+ Theler cell count, blood testosterone, blood cAMP and blood cortisol. 40 Sprague-Dawley male rats were divided into 5 groups(Normal, Control, SampleⅠ, SampleⅡ, SampleⅢ), 6 animals in every group. Normal group was not treated anything, control group was administrated normal saline in the same desage of sample Ⅰ. 3 Sample groups were received some of Yookmijihwangrang water abstract at one time per 24 hours during 5 days in different dosage. Sample Ⅰ (1/3 10pack/ml), SampleⅡ (1/62pack/ml), SampleⅢ(1/2.4pack/ml). After finishing treatment, all experimental svbjects were killed for blood sample on RBC, WBC, blood CD4+ T helper cell count, spleen CD4+ T helper cell count, axillary lymph node CD4+ T helper cell count. .blood cAMP, blood testosterone and blood cortisol. The results were as follows: RBC and WBC were incrased in all sample groups. Blood CD4+ Thelper cell count(CD4+ T cell count in the blood/whole lymphocyte count in the blood ×100%) was Mormal 46.17±5.88. Control 44.50±4.37, Sample Ⅰ 53.00±2.28. Sample Ⅱ 53.83±3.87, SampleⅢ 52.17±2.93. By the 95% Duncan ANOVA all experimental groups(sampleⅠ, sampleⅡ, sampleⅢ) showed slight significant difference from Nornal and Control. Blood cAMP(nmd/l) were Normal 1.12±0.17, Control 1.16±0.32, SampleⅠ 0.46±0.07, SampleⅡ 0.44±0.04, SampleⅢ 0.54±0.04 All cxperimental groups were signigicantly different from both Normal and Control groups(p<0.05). Blood cortisol(m l/ml) were Normal 100.00±2.00, Control 90.00±4.00, Samplc Ⅰ 440.00±5.00, SampleⅡ 520.00±40.00, SampleⅢ 470 00±7.00, Blood cortisol of all experimental groups were signifieantly increased(p<0.05). The results suggest that Yookmijihwangiang water abstract could be administrated to patienrts who have some diseases insufficient essence.

육미지황탕(六味地黃湯)이 생리활성지표(生理活性指標)와 임파구세포수(淋巴球細胞數)에 미치는 영향(影響)

김영권,류봉하,박동원,류기원,Kim, Young-Kuen,Ryu, Bong-Ha,Park, Dong-Won,Ryu, Ki-Won 대한암한의학회 1998 大韓癌韓醫學會誌 Vol.4 No.1

Yookmijihwangtang has been widely used oriental herb prescriptions, which is healing some discuss that come from insufficiency of innate essence and deficiency of kidney Ki. The meaning of healing discusses tonification of insufficient innate essence and insufficient kidney Ki can be regarded as reinforcement of wholely power of keeping homeostasis, that is correlated with immuno-responsibility which protects subject from outer antigen to keep normal vital condition. This study was aimed to investigate correlative effects of Yookmijihwangtang water abstract on the RBC, WBC, blood CD4+ T helper cell count, blood testosterone, blood cAMP and blood cortisol. 40 Sprague-Dawley male rats were divided into 5 groups(Normal, Control, Sample I, Sample II, Sample III), 6 animals in every group. Normal group was not treated anything, control group was administrated normal saline in the same dosage of Sample I. 3 Sample groups were received some of Yookmijihwangtang water abstract at one time per 24 hours during 5 days in different dosage. Sample I(1/310pack/ml), Sample II(1/62pack/ml), Sample III(1/2.4pack/ml). After finishing treatment, all experimental subjects were killed for blood sample on RBC, WBC, blood CD4+ T helper cell count, spleen CD4+ T helper cell count, axillary lymph node CD4+ T helper cell count. blood cAMP, blood testosterone and blood cortisol. The results were as follows; RBC and WBC were increased in all sample groups. Blood CD4+ T helper cell count(CD4+ T cell count in the blood/whole lymphocyte count in the blood ${\times}100%$) was Normal $46.17{\pm}5.88$, Control $44.50{\pm}4.37$, Sample I $53.00{\pm}2.28$, Sample II $53.83{\pm}3.87$, Sample III $52.17{\pm}2.93$. By the 95% Duncan ANOVA all experimental groups(sample I, Sample II, Sample III) showed slight significant difference from Normal and Control. Blood cAMP(nmol/l) were Normal $1.12{\pm}0.17$, Control $1.16{\pm}0.32$, Sample I $0.46{\pm}0.07$, Sample II $0.44{\pm}0.04$, Sample III $0.54{\pm}0.04$. All experimental groups were singificantly different from both Normal and Control groups(p<0.05). Blood cortisol(nl/ml) were Normal $100.00{\pm}2.00$ Control $90.00{\pm}4.00$, Sample I $440.00{\pm}5.00$, Sample II $520.00{\pm}40.00$, Sample III $470.00{\pm}7.00$. Blood cortisol of all experimental groups were significantly increased(p<0.05). The results suggest that Yookmijihwangtang water abstract could be administrated to patients who have some diseases insufficient essence.

고도비만 환자에서 복강경 위소매절제술 후 발생한 누공의 내시경 치료 1예

류민선,심기남,조원영,김찬용,강현주,김미연,안소영,이윤표,조형원,정성애,이주호 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.2

Laparoscopic sleeve gastrectomy can reduce morbidity and mortality in patients with morbid obesity, but it can cause complications such as a gastrointestinal leak. A 30-year-old morbidly obese female who had type 2 diabetes mellitus and hypertension with estimated body mass index of 40.2 kg/m2 was admitted. Laparoscopic sleeve gastrectomy was performed. On postoperative day 19, a leak was suspicious on physical examination and radiologic findings. Conservative management was performed, but the patient was hemodynamically unstable and imminently septic. After laparoscopic drainage procedure, esophagogastroduodenoscopy was performed and revealed the fistula opening at staple line just below gastroesophageal junction. Fibrin tissue adhesive was injected around the fistula and the esophageal covered stent was inserted to cover the leak. At 14th days after stent insertion, the barium study confirmed no more leak. In this case, we experienced that the esophageal stent insertion with fibrin tissue adhesive injection may reduce recovery time of the fistula developed after laparoscopic sleeve gastrectomy

21C의 컴퓨터 敎育

류기한,최영규 충주대학교 학생생활연구소 1998 學生生活硏究 Vol.5 No.-

형광검출 역상 액체크로마토그래피에 의한 Histamine의 정량

유희춘,김형룡,김상현,김대기,이영미,김형민,안년형,신태용 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

Histamine을 정확하고 신속하게 정량하기 위해 9.Fiuorenylmethyl chloroformate를 형광유도체화제로 하여 역상 HPLC법으로 정량하였다. 히스타민을 형광유도체화할 때 반응액의 pH, 반응시간, 형광유도체화제의 농도 등 최적 반응 조건을 검토하였다. 이 방법으로 히스타민을 분석한 결과 0.1~0.5ug/ml의 농도범위에서 상관계수가 0.922인 양호한 직선성을 나타내었으며 검출한계는 0.01??/ml였다. A rapid and simple method for the determination of histamine by reverse-phase high performance liquid chromatography with fluorescence detection was established. 9-Fluorenylmethyl chloroformate(FMOC) was used as fluorescent derivative reagent. The optimum conditions for the derivatiation such as pH, reaction time and he concentration of FMOC were investigated. Linearity of calibration curve was obtained between 0.1ug/ml/and 0.5ug/ml(r=0.922) and the limit of detection was 0.01ug/ml.

保精湯의 抗癌 및 免疫增强效果에 關한 硏究

류기원,박동원,류봉하,김혜영 경희대학교 1998 慶熙醫學 Vol.14 No.3-4

大學 캠퍼스內 步行路 計劃을 爲한 硏究 : 成均館 大學校自然科學 캠퍼스의 步道 利用 行態 調査를 中心으로

徐紀英,金起中,柳垠朱 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.1

A well designed pedestrian circulation system is considered as one of the most important factor in successful campus planning. Especially the layout of the walkway is a key element which directly affects the efficiency of the system. The purpose of this study is to provide a set of the guidelines that would help designing pedestrian roads in university campus. Tracking down the footpaths around the campus(Sung Kyun Kwan University, Natural Sciences Campus) was the main method of the survey to define the existing conditions and the problems. Selected traces were closely observed and analysed. In an effort to generate the design guidelines, proper solutions were given to each cases in accordance with the characters of the traces.

과학교육 프로그램의 비교ㆍ분석 : Centered on SCIS, ESS, SAPA and PKA Program SCIS,ESS,SAPA,PKA 프로그램을 중심으로

윤기영,류칠선 서원대학교 교육연구소 1989 敎育發展 Vol.8 No.1

The Purpose of this study is to offer basic research for process oriented science curriculum through comparing and analyzing of SCIS, ESS, SAPA Program developed early 1960's and PKA Program develoged late 1970's. Concrete research questions are as follows : 1.What are the characteristics of theoretical backgrounds, aims, Contents and methods of SCIS, ESS, SAPA and PKA ? 2.What are the common points and the differences of SCIS, ESS, SAPA and PKA ? Research results of each question are as follows : First, each program is composed according to it's own theoretical back grounds, aims, contents and methods. The general trend is SCIS, ESS, SAPA and PKA commonly empasized the education of process oriented, espacially ESS and PKA scientific attainments capacity. Second, the common point and differences of SCIS, ESS, SAPA, and PKA appeared variously in its kind. ESS and PKA both empasized the development of children, while SCIS and SAPA both empasized the acquisition of scientific contents in the educational purpose. The teachers in SCIS, ESS, SAPA used teacher-oriented teaching method, while PKA used child-oriented teaching method.

RF sputtering을 이용한 BaTiO_3 세라믹 박막의 제조 및 특성에 관한 연구

이영희,류기원 광운대학교 신기술연구소 1997 신기술연구소논문집 Vol.26 No.-

본 연구에서는 RF sputtering을 이용하여 Pt/SiO_2/Si(100) 기판 위에 BaTiO_3 박막을 제조하였으며, 박막의 구조적, 결정학적 특성을 증착조건과 열처리온도에 따라 조사하였다. 최적의 Ar/O_2 gas 비, sputtering 압력, RF power, 그리고 열처리온도는 각각 4/1, 12[mTorr], 120[W] 그리고, 650℃ 1시간이었다. 이때 박막의 증착율은 0.75[Å/sec]였다. X선 회절분석의 결과로부터 최적조건에서 증착된 BaTiO_3 박막은 강유전상을 나타내었으며 유전상수와 유전손실은 각각 683, 0.05였다. In this paper, BaTiO_3 thin film on Pt/SiO_2/Si(100) substrates were prepared by RF sputtering, whose structural, crystallographic properties were examined according to the deposition conditions and annealing temperatures. The optimum Ar/O_2 gas flow rate, sputtering pressure, RF power and annealing temperature were 4/1, 12[mTorr], 120[W] and 650[℃] for 1[hr], respectively. The growth rate of deposition film was about 0.75[A /sec]. From the results of X-ray diffraction pattern, BaTiO_3 thin film deposited at the optimum conditions was a ferroelectric phase. The dielectric constant and loss were about 683 and 0.05, respectively.