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- 저자 : Qingli Lin (검색결과 299 건)
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Qingli Lin,Yi-Yan Lu,Ling Hu,Xiaobo Zhao 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.2
To achieve a rapid and great enhancement in the fl exural performance of reinforced concrete beams, this study proposes a new strengthening method using U-shaped steel casing and focuses on studying the fatigue performance of strengthened beams. In this paper, ten specimens were tested under fatigue loads. Testing results demonstrated that no obvious damage was observed from the strengthened beam after 2 million fatigue cycles compared with the reference beam which failed at 58.97 × 10 4 fatigue cycles, indicating the good joint performance of the U-shaped steel casing and the RC beam. The strengthened beams generally failed by the sudden rupture of the bottom steel plate accompanied by concrete crushing. The mid-span defl ection and material strains all increased rapidly at the beginning, then steadily developed during nearly 80% of the fatigue life, and grew rapidly again after the weld toe cracking until the strengthened beam failed. The fatigue life was signifi cantly extended with the increase of either the height of the steel box or the thickness of the bottom plate, while it was dramatically shortened with the increase in the fatigue load amplitude. To facilitate the use of the outcomes, a simplifi ed formula for predicting the concrete residual strain of strengthened beams was derived. The predicted results matched the experimental ones well. The derived formula is theoretically only applicable to strengthened beams with dimensions same as the specimens in the experimental tests under similar loading conditions. Reasonably good agreements were found between the fi nite element analyses and experimental results. The developed fi nite element model can well simulate the failure mode under static load and the damage evolution under fatigue load. Based on the stress analysis results, the fatigue lives of the strengthened beams were predicted relatively accurately using the Fe-safe software, with the deviations mostly less than 15%.
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Phenolic glycosides from the rhizomes of Cyperus rotundus and their antidepressant activity
Lin, San-qing,Zhou, Zhong-liu,Zhang, Hua-lin,Yin, Wen-qing 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.5
Depression is the most common psychiatric comorbidity in patients. Searching for antidepressant drugs from natural products is very important for the treatment of this disease. The ethanol extract and its fractions of Cyperus rotundus were evaluated for antidepressant activities in mice by our group. The aim of this study was to investigate the potential active components of C. rotundus. The extraction and solvent partition of the rhizomes of C. rotundus, and repeated column chromatography for n-BuOH fraction yielded three new phenolic glycosides, rotunduside D (1), rotunduside E (2), and rotunduside F (3). Their chemical structures were identified on the basis of spectroscopic data analyses including NMR, MS, UV, and IR. The potential antidepressant activity of all the isolated compounds was evaluated. In the despair mice models, rotunduside F (3) showed significant antidepressant activity at the dosage of 50 mg/kg, which was close to the positive control fluoxetine (20 mg/kg).
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Phenolic glycosides from the rhizomes of Cyperus rotundus and their antidepressant activity
San-Qing Lin,Zhong-Liu Zhou,Hua-lin Zhang,Wen-Qing Yin 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.5
Depression is the most common psychiatric comorbidity in patients. Searching for antidepressant drugs from natural products is very important for the treatment of this disease. The ethanol extract and its fractions of Cyperus rotundus were evaluated for antidepressant activities in mice by our group. The aim of this study was to investigate the potential active components of C. rotundus. The extraction and solvent partition of the rhizomes of C. rotundus, and repeated column chromatography for n-BuOH fraction yielded three new phenolic glycosides, rotunduside D (1), rotunduside E (2), and rotunduside F (3). Their chemical structures were identified on the basis of spectroscopic data analyses including NMR, MS, UV, and IR. The potential antidepressant activity of all the isolated compounds was evaluated. In the despair mice models, rotunduside F (3) showed significant antidepressant activity at the dosage of 50 mg/kg, which was close to the positive control fluoxetine (20 mg/kg).
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( Ji Lin Qing ),( Hai Bing Xiao ),( Lin Zhao ),( Gui Fang Qin ),( Li Hua Hu ),( Zhi Zhong Chen ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
TIM-1 (also known as KIM-1 and HAVcr-1) is a type I transmembrane glycoprotein member of the TIM family that may play important roles in innate and adaptive immune responses. The overexpression of proteins associated with membrane proteins is a major obstacle to overcome in studies of membrane protein structures and functions. In this study, we successfully coupled the overexpression of the TIM-1 protein with a C-terminal enhanced green fluorescent protein (GFP) tag in Escherichia coli. To the best of our knowledge, this report is the first to describe the overexpression of human TIM-1 in E. coli. The purified TIM-1-EGFP fusion protein recognized and bound directly to apoptotic cells and did not to bind to viable cells. Furthermore, we confirmed that the interactions of TIM-1-EGFP with apoptotic cells were blocked by TIM-1-Fc fusion proteins. This fusion protein represents a readily obtainable source of biologically active TIM-1 that may prove useful in future studies of human TIM-1.
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Lin Tingting,Ge Yuanyuan,Gao Qing,Zhang Di,Chen Xiaofeng,Hu Yafang,Fan Jun 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.12
Flavin mononucleotide-binding proteins or domains emit cyan-green fluorescence under aerobic and anaerobic conditions, but relatively low fluorescence and less thermostability limit their application as reporters. In this work, we incorporated the codon-optimized fluorescent protein from Chlamydomonas reinhardtii with two different linkers independently into the redox-responsive split intein construct, overexpressed the precursors in hyperoxic Escherichia coli SHuffle T7 strain, and cyclized the target proteins in vitro in the presence of the reducing agent. Compared with the purified linear protein, the cyclic protein with the short linker displayed enhanced fluorescence. In contrast, cyclized protein with incorporation of the long linker including the myc-tag and human rhinovirus 3C protease cleavable sequence emitted slightly increased fluorescence compared with the protein linearized with the protease cleavage. The cyclic protein with the short linker also exhibited increased thermal stability and exopeptidase resistance. Moreover, induction of the target proteins in an oxygen-deficient culture rendered fluorescent E. coli BL21 (DE3) cells brighter than those overexpressing the linear construct. Thus, the cyclic reporter can hopefully be used in certain thermophilic anaerobes.
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Qing-Xin Ren,Chao Hou,Dennis Lam,Lin-Hai Han 국제구조공학회 2014 Steel and Composite Structures, An International J Vol.17 No.5
Tapered concrete filled double skin steel tubular (CFDST) columns have been used in China for structures such as electricity transmission towers. In practice, the bearing capacity related to the connection details on the top of the column is not fully understood. In this paper, the experimental behaviour of tapered CFDST stub columns subjected to axial partial compression is reported, sixteen specimens with top endplate and ten specimens without top endplate were tested. The test parameters included: (1) tapered angle, (2) top endplate thickness, and (3) partial compression area ratio. Test results show that the tapered CFDST stub columns under axial partial compression behaved in a ductile manner. The axial partial compressive behaviour and the failure modes of the tapered CFDST stub columns were significantly influenced by the parameters investigated. Finally, a simple formula for predicting the cross-sectional capacity of the tapered CFDST sections under axial partial compression is proposed.
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Cryptosporidium suis Infection in Post-Weaned and Adult Pigs in Shaanxi Province, Northwestern China
Qing Lin,Xing-Ye Wang,Jian-Wen Chen,Ling Ding,Guang-Hui Zhao 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1
Cryptosporidium spp., ubiquitous enteric parasitic protozoa of vertebrates, recently emerged as an important cause of economic loss and zoonosis. The present study aimed to determine the distribution and species of Cryptosporidium in post-weaned and adult pigs in Shaanxi province, northwestern China. A total of 1,337 fresh fecal samples of postweaned and adult pigs were collected by sterile disposable gloves from 8 areas of Shaanxi province. The samples were examined by Sheather’s sugar flotation technique and microscopy at×400 magnification for Cryptosporidium infection, and the species in positive samples was further identified by PCR amplification of the small subunit (SSU) rRNA gene. A total of 44 fecal samples were successfully amplified by the nested PCR of the partial SSU rRNA, with overall prevalence of 3.3%. The average prevalence of Cryptosporidium infection in each pig farms ranged from 0 to 14.4%. Species identification by sequencing of SSU rRNA gene revealed that 42 (3.1%) samples were Cryptosporidium suis and 2 (0.15%) were Cryptosporidium scrofarum. C. suis had the highest prevalence (7.5%) in growers and the lowest in breeding pigs (0.97%). C. suis was the predominant species in pre-weaned and adult pigs, while C. scrofarum infected pigs older than 3 months only. A season-related difference of C. suis was observed in this study, with the highest prevalence in autumn (5.5%) and the lowest (1.7%) in winter. The present study provided basic information for control of Cryptosporidium infection in pigs and assessment of zoonotic transmission of pigs in Shaanxi province, China.
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Qing Ni,Li Sun,Ensi Cao,Wentao Hao,Yongjia Zhang,Lin Ju 한국물리학회 2020 Current Applied Physics Vol.20 No.9
(Li1+, Al3+) co-doped Ni0.5Zn0.5Fe2O4 ferrites, Ni0.5-xZn0.5-xLixAlxFe2O4 (x = 0.000, 0.025, 0.050 and 0.100), were synthesized by the sol-gel auto-combustion method. X-ray diffraction (XRD), field emission scanning electronic microscope (FESEM), vibrating sample magnetometer (VSM) and LCR meter were used to investigate the structural, magnetic and dielectric properties. Results of XRD and SEM indicate that both doping amount and calcination temperature play significant roles in crystal structure and grain growth. Also, it can be observed that the saturation magnetization and the coercivity change in a noticeable manner. The Ni0.475Zn0.475Li0.025Al0.025Fe2O4 ferrite sintered at 1200 °C has a relatively low coercivity value (62.93 Oe) and the largest saturation magnetization (110.95 emu/g). Besides, dielectric behavior is also improved by Li1+ and Al3+ co-doping
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Qing-Feng Meng,Wei-Lin Wang,Xiao-Ting Ni,Hai-Bin Li,Gui-Zhe Yao,Xiao-Lin Sun,Wei-Li Wang,Wei Cong 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.6
The breeding of domestic rabbits (Oryctolagus cuniculus) for human consumption has a long tradition in China. Infections that can affect the production of meat or even be transmitted from animals to humans are important to monitor, especially for public health reasons as well as for their impact on animal health. Thus, a total of 1,132 domestic rabbit sera from 4 regions in China were collected for serological screening for Encephalitozoon cuniculi and for Toxoplasma gondii by ELISA and modified agglutination test (MAT), respectively. Antibodies to E. cuniculi were detected in 248/1,132 (21.9%) sera tested while antibodies against T. gondii revealed a seroprevalence of 51/1,132 (4.5%). We believe that the present results are of epidemiological implications and public health importance due to the acknowledged susceptibility of humans to E. cuniculi and T. gondii infections. Therefore, routine screening tests of domestic rabbits are proposed considering the zoonotic potential of these parasites.
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