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Effect of Microstructure on Mechanical Property of Carbon Steel/Strainless Steel Rolling Clad Plate
Li Hai-bin,Zhou Cun-long,Fan Xiao-ning,Kong Yi-gang,Huang Qing-xue,Ma Qin 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.11
The bonding interface microstructure and mechanical property of clad plate with different hot rolling schedules were researched. The results show that the carbide layer thickness of bonding interface increases firstly and then decreases with increasing reduction ratio. The carbide layer of bonding interface is the major factor relating to tensile strength, so the experimental values of clad plate are slightly higher than the calculated ones. Moreover, the bending strength of clad plate is mainly influenced by the thickness ratio and the carbide quantity of SS, while the carbide layer thickness of bonding interface is an important factor affects the bending strength.
Vibration of nonuniform carbon nanotube with attached mass via nonlocal Timoshenko beam theory
Hai-Li Tang,Zhi-Bin Shen,Dao-Kui Li 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.9
This paper studies the vibrational behavior of nonuniform single-walled carbon nanotube (SWCNT) carrying a nanoparticle. A nonuniformcantilever beam with a concentrated mass at the free end is analyzed according to the nonlocal Timoshenko beam theory. Agoverning equation of a nonuniform SWCNT with attached mass is established. The transfer function method incorporating with theperturbation method is utilized to obtain the resonant frequencies of a vibrating nonlocal cantilever-mass system. The effects of thenonlocal parameter, taper ratio and attached mass on the natural frequencies and frequency shifts are discussed. Obtained results indicatethat the sensitivity of the frequency shifts on the attached mass increases when the length-to-diameter ratio decreases. Tapered SWCNTpossesses higher fundamental frequencies if the taper ratio becomes larger.
Cordblood-Based High-Throughput Screening for Deafness Gene of 646 Newborns in Jinan Area of China
Shou-Xia Li,Ding-Li Chen,Su-Bin Zhao,Li-Li Guo,Hai-Qin Feng,Xiao-Fang Zhang,Li-Li Ping,Zhi-Ming Yang,Cai-Xia Sun,Gen-Dong Yao 대한이비인후과학회 2015 Clinical and Experimental Otorhinolaryngology Vol.8 No.3
Objectives. Infants with slight/mild or late-onset hearing impairment might be missed in universal newborn hearing screening (UNHS). We identified the mutation hot spot of common deaf gene in the newborns in Jinan area population by screening the mutation spot with neonate cord blood, in order to make clear whether the neonate cord blood for screening is feasible. Methods. Six hundred and forty-six newborns were subjected to both UNHS and genetic screening for deafness by using neonate cord blood. The newborn genetic screening targeted four deafness-associated genes, which were commonly found in the Chinese population including gap junction beta-2 protein (GJB2), gap junction beta-3 protein (GJB3), solute carrier family 26 member 4 (SLC26A4), and mtDNA 12S rRNA. The most common 20 spot mutations in 4 deaf genes were detected by MassARRAY iPLEX platform and mitochondrial 12S rRNA A1555G and C1494T mutations were sequenced using Sanger sequencing. Results. Among the 646 newborns, 635 cases passed the UNHS and the other 11 cases (1.7%) did not. Of the 11 failures, two cases were found to carry homozygous GJB2 p.R143W pathogenic mutation, one case was found to have heterozygous GJB2 235delC mutation, and another one case carried heterozygous GJB3 p.R180X pathogenic mutation. Six hundred and thirty-five babies passed the newborn hearing screening, in which 25 babies were identified to carry pathogenic mutations, including 12 heterozygotes (1.9%) for GJB2 235delC, eight heterozygotes (1.3%) for SLC26A4 IVS7-2A>G, one heterozygote (0.2%) for p.R409H, two homozygotes (0.3%) for m.1494C>T, and two homozygotes (0.3%) for m.1555A>G. Conclusion. Newborn genetic screening through the umbilical cord blood for common deafness-associated mutations may identify carriers sensitive to aminoglycoside antibiotic, and can effectively prevent or delay hearing loss occurs.
Bin Liang,Xue-Hai Gao,Le Pan,Zhi-Heng Li,Ying-Chun Zhang 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.6
A space robotic system is expected to perform on-orbit servicing missions to rescue malfunctionedsatellites in geostationary orbit (GEO). In final berthing and capture, it is difficult for a space robot to determine therelative pose (attitude and position) of a non-cooperative malfunctioned satellite that is usually huge and withoutartificial recognition devices. In this paper, a space robot with a monocular structured light vision subsystem isintroduced to solve the problem. Firstly, the monocular structured light vision subsystem composed of a singlecamera and a point light source is designed. Secondly, a partial rectangular shaped framework, which is verycommon on a non-cooperative malfunctioned satellite, is chosen as the recognition object for non-cooperative posemeasurement. Using projection constraints on rectangle and circular points, a rectangle feature reconstructionalgorithm is proposed. Thirdly, according to the reconstructed rectangle feature, a least square method of posedetermination is presented. Lastly, using a semi-physical vision simulation system, several experiments of typicalcases are simulated to verify the pose determination method of large non-cooperative target. The results show thevalidity and flexibility of the proposed method.
Li, Xiu-Juan,Zha, Quan-Bin,Xu, Xin-Yu,Xia, Lei,Zhang, Zhe,Ren, Zhao-Jun,Tang, Jin-Hai Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22
Inflammatory breast cancer (IBC) is a rare, aggressive form of breast cancer which is more likely to be her-2/neu amplified. While the her-2/neu status has been utilised to predict prognosis, the published data are inconsistent. The present meta-analysis was conducted to determine whether the her-2/neu status predicts outcomes. Papers were selected from the PubMed database based on defined inclusion and exclusion criteria. Parameters such as total patients, follow-up time and outcome statistics (i.e. overall survival (OS), relapse-free survival (RFS) were collected. The analysis included 6 studies with 2,838 IBC patients. The summary hazards ratio (HR) estimating the association of OS with HER-2-positive disease was 0.96 (95% confidence interval (95%CI: 0.85-1.10)), with similar findings for RFS (HR=0.81, 95%CI: 0.61-1.09). No obvious statistical heterogeneity was detected. This meta-analysis suggests that HER-2-positive status is not an independent adverse prognostic factor for survival among IBC patient cases.
Li, Chen-Long,Chang, Liang,Guo, Lin,Zhao, Dan,Liu, Hui-Bin,Wang, Qiu-Shi,Zhang, Ping,Du, Wen-Zhong,Liu, Xing,Zhang, Hai-Tao,Liu, Yang,Zhang, Yao,Xie, Jing-Hong,Ming, Jian-Guang,Cui, Yu-Qiong,Sun, Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23
Background: ${\beta}$-elemene, extracted from herb medicine Curcuma wenyujin has potent anti-tumor effects in various cancer cell lines. However, the activity of ${\beta}$-elemene against glioma cells remains unclear. In the present study, we assessed effects of ${\beta}$-elemene on human glioma cells and explored the underlying mechanism. Materials and Methods: Human glioma U87 cells were used. Cell proliferation was determined with MTT assay and colony formation assay to detect the effect of ${\beta}$-elemene at different doses and times. Fluorescence microscopy was used to observe cell apoptosis with Hoechst 33258 staining and change of glioma apoptosis and cell cycling were analyzed by flow cytometry. Real-time quantitative PCR and Western-blotting assay were performed to investigated the influence of ${\beta}$-elemene on expression levels of Fas/FasL, caspase-3, Bcl-2 and Bax. The experiment was divided into two groups: the blank control group and ${\beta}$-elemne treatment group. Results: With increase in the concentration of ${\beta}$-elemene, cytotoxic effects were enhanced in the glioma cell line and the concentration of inhibited cell viability ($IC_{50}$) was $48.5{\mu}g/mL$ for 24h. ${\beta}$-elemene could induce cell cycle arrest in the G0/G1 phase. With Hoechst 33258 staining, apoptotic nuclear morphological changes were observed. Activation of caspase-3,-8 and -9 was increased and the pro-apoptotic factors Fas/FasL and Bax were upregulated, while the anti-apoptotic Bcl-2 was downregulated after treatment with ${\beta}$-elemene at both mRNA and protein levels. Furthermore, proliferation and colony formation by U87 cells were inhibited by ${\beta}$-elemene in a time and does-dependent manner. Conclusions: Our results indicate that ${\beta}$-elemene inhibits growth and induces apoptosis of human glioma cells in vitro. The induction of apoptosis appears to be related with the upregulation of Fas/FasL and Bax, activation of caspase-3,-8 and -9 and downregulation of Bcl-2, which then trigger major apoptotic cascades.
A Genetic Linkage Map Construction for Sesame (Sesamum indicum L.)
Li Bin Wei,Hai Yang Zhang,Yong Zhan Zheng,Hong Mei Miao,Tian Zhen Zhang,Wang Zhen Guo 한국유전학회 2009 Genes & Genomics Vol.31 No.2
Sesame (Sesamum indicum L.) is one of the oldest oilseed crops with high seed oil quality. The first sesame genetic linkage map based on F2 segregating population of an intraspecific cross between two cultivars was constructed. Using three types of PCR-based markers, 284 polymorphic loci including 10 EST-SSR marker, 30 AFLP marker and 244 RSAMPL marker, respectively, had been screened. Subsequently, a total of 220 molecular markers were mapped in 30 linkage groups covering a genetic length of 936.72 cM, and the average distance between markers was 4.93 cM. In this map, the linkage groups contained from 2 to 33 loci each and ranged in distance from 6.44 cM to 74.52 cM. Based on map information, sesame genome length was estimated to be approximately 1,232.53 cM, and genome coverage of this map was about 76.0%. As a starting point of sesame genome study, the genetic linkage map will be hopeful to tag traits of breeding interest and further aid in the sesame molecular breeding. Furthermore, RSAMPL marker had been also appreciated in this paper, for its first usage in genetic map construction and higher utilization potential in some crop species lacking much genome information.
Li, Hai,Dong, Bin,Park, Sahng Wook,Lee, Hyun-Sook,Chen, Wei,Liu, Jingwen American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.42
<P>PCSK9 is a natural inhibitor of LDL receptor (LDLR) that binds the extracellular domain of LDLR and triggers its intracellular degradation. <I>PCSK9</I> and <I>LDLR</I> are coordinately regulated at the transcriptional level by sterols through their promoter-imbedded sterol response elements (SRE) and co-induced by statins. Identification of regulatory networks modulating <I>PCSK9</I> transcription is important for developing selective repressors of PCSK9 to improve statin efficacy by prolonging the up-regulation of LDLR. Interestingly, the plant-derived hypocholesterolemic compound berberine (BBR) up-regulates LDLR expression while down-regulating PCSK9. In our investigations to define mechanisms underlying the transcriptional suppression of <I>PCSK9</I> by BBR in HepG2 cells, we have identified a highly conserved hepatocyte nuclear factor 1 (HNF1) binding site residing 28 bp upstream from SRE as a critical sequence motif for <I>PCSK9</I> transcription and its regulation by BBR. Mutation of the HNF1 site reduced <I>PCSK9</I> promoter activity >90%. A battery of functional assays identified HNF1α as the predominant trans-activator for <I>PCSK9</I> gene working through this sequence motif. We further provide evidence suggesting that HNF1 site works cooperatively with SRE as HNF1 mutation significantly attenuated the activity of nuclear SREBP2 to transactivate <I>PCSK9</I> promoter. Finally, we show that a coordinate modest reduction of HNF1α and nuclear SREBP2 by BBR led to a strong suppression of <I>PCSK9</I> transcription through these two critical regulatory sequences. This is the first described example of SREBP pairing with HNF1 to control an important regulatory pathway in cholesterol homeostasis. This work also provides a mechanism for how BBR suppresses <I>PCSK9</I> transcription.</P>