電氣相似模型에 依한 흙속의 浸透流 解析

權武男,具賢謨,李相昊,李錫健 慶北大學校 1989 論文集 Vol.47 No.-

This study was performed to evaluate the usefulness of the electrical analogue model in porous medium. Experimental values by the electrical analogue models were compared with those by the sand models and theoretical values by the fragment method. The results obtained are as follows: 1. The flow lines into the sand model were changed according to rising the upstream water level but those of the electrical analogue model weren't changed, and the equipotential lines were revealed to be almost same in the two kinds of models above. 2. The seepage quantities by the fragment method and the electrical analogue model were a little more than those by the sand model. 3. The exit gradients by the fragment method were steeper than those by the sand model and the electrical analogue model. 4. The seepage quantities were increased in proportion to the increment of the exit gradients. By means of the results above, it may be given as a conclusion that the electrical analogue model test is more useful than the sand model test for appling to seepage flow in the field condition, because the electrical analogue model test is more accurate and simpler. Then, the electrical analogue model test will be able to apply to the three-dimension seepage flow problems.

재래(흑색) 염소의 비육에 관한 연구 : Ⅰ. 일측 갑상선 제거가 체중, 체온 및 맥박에 미치는 영향 Ⅰ. Effect of Uni-lateral Thyroidectomy on Body Weight, Body Temperature and Pulse Rate

박무현,권해병,양일석 慶尙大學校 1975 論文集 Vol.14 No.-

In order to investigate the relationship of body weight, body temperature and pulse rate to hypothyroidism in the state of uni-lateral thyroidectomy of Korean native goat, a brief observation was carried out. Twenty healthy male adult goats (7 months old) were used in the present experiment dividing into 2 groups, the control with 5 and the experimental with 15, respectively. Individuals of the experimental group were received uni-lateral thyroidectomy by surgical operation, while individuals of the control group, on the other hand, were received sham-operation. Measurements of body weight, body temperature and pulse rate after the operation were performed in duration of 48 days by 2 days interval. The results obtained could be summerized as follows. 1. There were no significant changes in body weight of experimental group compared to that of control group. 2. Uni-laterally thyroidectomized goat showed no changes in body temperature, which is seemed to he doe to a compensatory effect. 3. Statistical calculation did not show any difference in pulse rate between control and experimental group. The present experiment can he concluded that uni-lateral thyroidetomy had not any effect on the changes body weight, body temperature and pulse rate.

조혈모세포이식 후 골성장인자의 변화 및 골대사에 미치는 영향 : Impact on Bone Mineral Metabolism

백기현,오은숙,오기원,이원영,김혜수,권순용,한제호,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2002 Endocrinology and metabolism Vol.17 No.5

연구배경: 각종 장기이식의 시행이 많아지고 이식 후 생존율이 증가함에 따라 이식 후 합병증에 대한 관심 또한 높아지고 있다. 조혈모세포이식 후에도 다양한 내분비적 합병증이 발생할 수 있으며 골격에 대한 합병증도 문제점으로 대두되고 있다. 조혈모세포 이식 후 발생하는 골소실에는 이식 후 초기의 골형성 저하와 골흡수 증가가 중요한 역할을 담당하리라고 추측되는데 이러한 골재형성불일치(biochemical uncoupling)에 골 성장인자들이 미치는 영향에 대해서는 알려진 바가 없다. 본 연구에서는 조혈모세포이식 전, 후로 말초 혈액에서 IGF-I, FGF-2, M-CSF같은 성장인자의 변화를 알아보고, 이들 성장인자의 변화가 조혈모세포이식 후의 골형과 골흡수에 미치는 영향 및 이식 후 발생되는 골량 소실과의 연관성을 확인해 보고자 하였다. 방법: 여러 가지 혈액질환으로 인해 동종 골수이식을 시행 받은 환자들을 전향적으로 관찰하였으며 이식 전 및 이식 후 1주, 2주, 3주, 4주 및 3개월, 6개월 1년에 말초 혈액에서 골교체표지자를 측정하였다. 이식 전 및 이식1년 후에 요추골 및 대퇴골 골밀도를 측정할 수 있었던 36명의 환자들을 대상으로 냉동 보관되어 있던 혈청을 이용하여 IGF-I, FGF-2 및 M-CSF를 시기별로 측정하였으며 이들 성장인자와 골교체표지자의 변화 및 골밀도 변화 사이의 상관관계를 확인하였다. 결과: 골흡수 표지자인 혈청 ICTP는 이식 전에 비해 이식 후 4주까지 점차 의의 있게 증가하다가 이후 6개월까지 더욱 증가한 후 감소하였다. 골형성 표지자인 osteocalcin은 이식 후 3주까지는 점차 감소하다가 이후 증가하여 이식 후 3개월 및 6개월에 기저치보다 통계적으로 유의하게 증가한 후 감소하였다. 혈청 IGF-I과 FGF-2는 각각 이식 후 3주 및 1주까지 의미있게 감소하다가 이후 증가하였으며 혈청 M-CSF는 이식 후 1주째에 기저치에 비해 의미 있게 증가하였다가 이후 기저치로 회복되었다. 이식 1년 후 평균 요추부 골밀도는 5.2% 감소하였고 평균 근위대퇴골 골밀도는 11.6% 감소하였다. 이식 전 및 이식 후 3주에 측정한 IGF-I과 같은 시기에 측정한 오스테오칼신 사이에 유의한 상관관계가 관찰되었으며 이식 후 3주째의 M-CSF와 골흡수표지자인 M-CSF 사이에서 의미 있는 양의 상관관계를 관찰할 수 있었다. 이식 후 3주 및 3개월에 IGF-I이 낮은 환자일수록 이식 1년 후 근위대퇴골에서의 골소실이 많은 것으로 분석되었다. 결론: 조혈모세포이식 후 발생하는 골소실에는 기존에 알려진 기저질환의 영향, 성호르몬의 감소, 면역억제의 투여, 골수기질세포와 조골세포의 손상 및 이식초기 사이토카인의 변화이외에도 골성장인자가 관련이 있음을 확인하였고, 이는 이식 후 발생되는 골량소실에 중요한 역할을 할 것이라고 사료된다. Background: A loss of bone mass is usually detected after a bone marrow transplantation (BMT), especially during the early post-transplant period. We recently reported that enhanced bone resorption following a BMT was related to both the steroid dose and the increase in IL-6. We also suggested damage to the marrow stromal microenvironment, by myoablation, partly explains the impaired bone formation following a BMT. It is well known that some growth factor play important role in bone growth and osteogenesis. However, the pathogenetic role of bone growth factors in post-BMT bone loss is unknown and data on the changes in the growth factors, in accordance with bone turnover markers and bone mineral density (BMD) changes are scarce. We investigated changes in bone growth factors such as IGF-I (Insulin-like growth factor-I), fibroblast growth factor-2 (FGF-2) and Macrophage colony stimulating factor (M-CSF), during the post-BMT period, and assessed whether the growth factor changes influenced the bone turnover and post-BMT bone loss. The present study is the first prospective study to describe the changes in bone growth factors following a BMT. Methods: We prospectively investigated 110 patients undergoing a BMT, and analyzed 36 patients (32.4±1.3 years, 17 men and 19 women) whose BMDs were measured before, and 1 year after, the BMT. The serum biochemical markers of bone turnover were measured before, 1, 2, 3 and 4 weeks, 3 and 6 months, and 1 year, after the BMT. The serum, FGF-2, IGF-I and M-CSF levels were measured before and 1 and 3 weeks, and 3 months after the BMT. The correlation between the changes of growth factors and various bone parameters was analyzed. Results: The mean bone losses in the lumbar spine and total proximal femur, calculated as the percentage change from the baseline to the level at 1 year, were 5.2(p<0.05) and 11.6%(p<0.01), respectively. the serum type I carboxyterminal telopeptide(ICTP), a bone resorption marker, increased progressively until 6 months after the BMT, but thereafter decreased, to the base value after 1 year. Serum osteocalcin, a bone formation marker, decreased progressively, until 3 weeks after the BMT but then increased transiently, and finally returned to the base level at 1 year. The serum IGF-I and FGF-2 also decreased progressively until 3 weeks 1 week after the BMT, respectively, then increased to the base values at 3 months. The serum M-CSF increased briskly at 1 week post-BMT, then decreased to the base level. There were positive correlations between the percentage changes from the baseline proximal femur BMD and the IGF-I levels 3 weeks and 3 months (r=0.52, p<0.01, r=0.41, p<0.05) post BMT. A significant correlation was found between the IGF-I and osteocalcin levels pre-BMT, and 3 weeks after the BMT. Another positive correlation was found between the M-CSF and the ICTP levels at 3 weeks post BMT (r=0.54, p<0.05). Conclusion: In conclusion, there were significant changes in the serum IGF-I, FGF-2 and M-CSF levels in the immediate post-BMT period, which were related to a decrease in bone formation and loss in the proximal femoral BMD during the year following the BMT (J Kor Soc Endocrinol 17:664∼674, 2002).

효소 중합반응에 의한 인공 cDNA 클론의 형성

유시현,권무식 成均館大學校 科學技術硏究所 1992 論文集 Vol.43 No.2

The sequence-specific DNA binding protein, PU.1 common to macrophage and B cell in mammals, is one of the transcription activators. Its DNA binding activity seems to correlate with the phosphorylation of specific serine residue(s). It is, however, not clear that which serine(s) could be phosphorylated for the activation of PU.1. To understand the phosphorylation mechanism of the transcription factor, the first nucleotide coding the 11th amino acid [TCC] of PU.1, "Thymidine", was substituted with "Guanidine" by site-directded mutagenesis. The mutant PU.1 cDNA restricted by Xba I and Hind III, was ligated into Xbal-HindIII cut pBluescript KS+, and then transformed into E.coli JM109 to generate a new mutant clone of PU.1. The mutant will be instrumental in evaluating a plausible phosphorylation site of PU.1.

조립 Filter내에서 세상의 이동현상에 대한 비교분석

金顯起,權武男 慶北大學校農業科學技術硏究所 1998 慶北大農學誌 Vol.16 No.-

본 실험에서는 수리구조물의 보호되는 토립자의 필터내 이동과 이로인한 필터의 기능 저하를 실험을 통하여 비교분석한 결과 다음과 같은 결론을 얻었다. 1. 수두가 높고 공극이 클수록 필터내 세사의 이동은 심했으며 조기에 흐름의 안정화에 도달하게 되었지만 필터외부로의 입자유출도 많았으며, 공극이 작고 유속이 빠르면 필터와 모래의 접촉면에 인접한 곳에서 Blocking현상이 크게 일어남을 알 수 있었다. 2. 필터기능에 영향을 주는 세사의 이동은 공극의 크기와 수두에 큰 영향을 받음을 알 수 있었고, USCE 필터와 USSPL 필터는 공극이 크기 때문에 같은 조건하에서 흐름의 안정이 다른 필터들 보다 조기에 이루어 졌다. 3. 세사의 이동잔류량은 USSPL, USCE, USBR, Newton & Hurley, Bertram 필터순으로 많이 나타났다. 4. 흐름의 안전에 소요된 시간은 Bertram, Newton & Hurley, USBR, USSPL, USCE 필터 순으로 적었고, 투수계수는 USBR, Bertram, Newton & Hurley, USSPL, USCE 필터순으로 크게 나타났다. 5. 여러 가지 종류의 필터기준중 USCE와 USSPL기준의 필터가 투수계수의 안정에 소요된 시간이 가장 짧고, 세사의 이동량이 적으므로 가장 적합한 것으로 인정된다. This experiment did comparison and analysis that protected soil particle migration have affect on function of the filter and therefore fall function of the filter. Results obtained are as follows: 1.High water head makes to be much movement of fine sand and out flow of particle to the outside. The filter have large opening size that reached stability an early stage, but much fine sand is washed away. If the velocity turns fast and becomes small, blocking phenomenon is remarkable nearby the filter-sand interface. 2. The movement of fine sand that effect on function of filter depend on opening size and change of water head. Under the same condition, USCE filter and USSPL filter is reached earlier than other filter that is stability of stage, because it's opening size is large. 3. Residual quantity of fine sand migration was largly come out in order of USSPL, USCE, USBR, Newton & Hurley, Bertram filter. 4. The time required to stability of flow was taken less in order of Bertram, Newton & Hurley, USBR, USSPL, USCE filter and coefficient of permeability was highly come out in order of USBR, Bertram, Newton & Hurley, USSPL, USCE filter. 5. It proved that USCE and USSPL is suitable for the filter criteria.

Mortierella nana의 균체지질중의 γ-Linolenic acid 함량에 미치는 비타민의 영향

박준권,임무현 대구대학교 산업기술연구소 2003 産業技術硏究 Vol.14 No.3

Mortiella nana속의 미생물을 이용한 균체지질중의 GLA생산에서 배양배지내 butyric acid, 수용성 및 지용성 비타민류를 첨가하여 이들 미량물질의 인자가 균의 생육, 지질생산 및 균체지질중의 지방산 변화와 GLA 함량변화에 미치는 영향을 검토하였다. Mortiella nana속의 배양에 각종 수용성비타민을 첨가한 결과, thiamin, riboflavin, niacinamide, pyridoxin의 첨가구에서 균체지질과 GLA함량이 높았다. 수용성 비타민중 thiamin의 농도를 달리하여 첨가한 결과 0.5㎍/㎖의 첨가구에서 지질함량 및 GLA 함량이 높게 나타났다. Ribofalvin, niacinamide, pyridoxin을 각각 농도별로 첨가한 경우 균체지질 함량 면에서 대조구보다 높았으나 GLA함량은 대조구보다 낮게 나타나, 균체지질의 생산에는 유리하게 작용하였지만 GLA의 생산에는 영향을 미치지 못하였다. n-alkane계의 butyric acid를 농도별로 첨가후 배양한 결과 05㎍/㎖의 농도의 첨가구에서 지질생산량은 0.43g이었고 GLA생산량에서는 18.5%로 무첨가구보다 2배이상의 생산량을 보여 균체지질 및 GLA생산에 큰 영향을 주는 것으로 나타났다. 지용성 비타민 중 tocopherol과 squalene을 배지에 첨가후 배양한 결과, 모든 농도의 첨가구에서 지질 생산량 및 GLA생산이 무첨가구보다 낮게 나타났다. 배양배지의 초기 pH의 영향을 조사한 결과 pH 6.0의 배양 처리구가 지질함량 0.45g, GLA생산량이 17.95%로 가장 최적의 초기배양 pH였으며 배지의 양과 배양속도를 달리하여 산소공급의 영향을 조사한 결과, 100rpm에서 150㎖의 배지로 배양한 처리구가 균체지질 및 GLA생산량이 높았으며, 배양 6일째 처리구가 균체 지질 및 GLA 생산성이 가장 높게 나타났다. The effects of water soluble and fat soluble vitamins on the content of γ-linolenic acid(GLA) in the mycelial lipid by culture of Mortiella nana, were investigated. Addition of water soluble vitamins, thiamin, riboflavin niacinamide and pyridoxin, in the culture medium of Mortiella nana resulted in the increase of mycelial lipid and GLA content, In the addition of 0.5㎍/㎖ thiamin to the basal medium, mycelial lipid and GLA content were the highest. In the each addition concentration of riboflavin niacimide and pyridoxin, mycelial content were more higher than control, but GLA content were more lower. It was addition of riboflavin niacinamide and pyridoxin found that mycelial lipid production was favorable, but GLA production was not affected. In the addition of 0.5㎍/㎖ butyric acid as the n-alkane to the basal medium containing 0.5㎍/㎖ thiamin, mycelial content was 0.43g/g dry cell weight and It was found 18.5% of GLA production that addition of 0.5㎍/㎖ was more twice higher than blank. It was appeared that addition of butyric acid was affected to mycelial lipid and GLA production. In the addition of fat soluble vitamin, tocopherol and squalene, mycelial lipid and GLA production were lower than blank at all added concentration. At the influence of intial pH of culture medium, pH 6.0 of culture medium was optimum, the following results were 0.45g of mycelium lipid and 17.95% of GLA production. The influence of oxygen applying with the various of medium volume and two shaking speed were also examed, When cultivation was cultured for 6 days with 150㎖ volume of medium at 100rpm, mycelium lipid and GLA production were the highest.

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

석회 흔입 점토의 강도 특성

여재호,권무남,구정민,김현기 경북대학교 농업과학기술연구소 2000 慶北大農學誌 Vol.18 No.-

This study was conducted to investigate most effective the optimum lime content for lime-clay modification. To achieve the aim, characteristics of compaction and compressive strength were tested by adding of 0, 5, 10, 15 and 20% lime (Hydrated lime) of dry weight of the clay. Distilled water was added 10, 15, 20 and 25% of dry weight of lime-clay mixture. In this test, the compressive strength of the specimens was measured according to the following curing period : 7, 21, 28, 35 and 49 days. The results are as follows. (1) As lime additive increased, the optimum moisture content of lime-clay mixture was increased and the maximum dry density was decreased. (2) the soil mixture of 20% of the moisture content and 10% of lime additive was shown the maximum compressive strength. (3) As curing period longer, the compressive strength was increased but after 21 curing days, the increasing rate of compressive strength was low as compared with earlier its value. (4) In the range of 20% of the moisture content, compressive strength of mixture of 10% lime additive increased twice compared with that of mixture of 0% lime additive. (5) All of the lime-clay are possible to use for an sub-base material and 20% of moisture content of lime-clay mixture is possible to use for a base material.

항혈청 투여에 따른 돼지 전염성 설사병 예방효과 : Ⅰ.혈청학적 결과, RT-PCR 검사, 형광항체검사 Ⅱ.Serological Results, Rt-PCR for Fecal and Small Intestin, FA Test

지영철,한정희,권혁무,한태욱,정현규,박봉균 한국수의병리학회 2002 한국수의병리학회지 Vol.6 No.1

The purpose of this study was to investigate to potective effects against porcine epidemic diarrhea virus (REDV) infection in piglets by administration of the PEDV antiserum orally at 2 hrs, 24 hrs and 36 hrs after birth. six piglets adiminstered the antiserum were experimentally infected with PEDV at five-day-old. Control group were four piglets infected with PEDV only. Serum antibody titers aginst PEDV were examined by serum neutralization (SN) test, dectection for PEDV or PEDV antigen from feces and small intestines was tested by reverse transcrption-polymerase chain reaction (RT-PCR) and indirect immunoflurescence (IFA). The results obtained were as follows; 1. The piglets administered the PEDV antiserum showed higher antibody titers than those of control group and sustained during the experimental period. 2. The detection rate of PEDV in feces and small intestines by RT-PCR were 26.2% and 16.7% in PEDV antiserum treated group and 48.1% and 75.0% in control group, respectively. 3. The detection rate of PEDV antigen in the small intestine by IFA were 0% in PEDV antiserum treated group and 50.0% in control group, respectively. It was concluded that oral administration of antiserum against PEDV to piglets was effective in preventing PEDV infection.

급냉 제강 슬래그 잔골재 대체율에 따른 차폐콘크리트의 공학적 특성에 관한 실험적 연구

김진만,조성현,권기주,김무한 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.3

The annual quarrying amount of sea sand in Korea occupies 55% of total fine aggregate. However, quarrying amount gradually decrease because environmental groups and neighboring fishermen have objected indiscreet quarrying which breaks inhabitation of living things in the sea and erodes the coast. To solve these problems, therefore, it is necessary to develope of sand being able to replace sea sand. On the other hand the steel slag, a by-product which is produced during the manufacture of steel by refining pig iron, has generated about four million tons yearly. It is difficult to recycle steel slag as aggregate for concrete because the reaction with water and free-CaO in steel slag could make the expansion of volume. However, steel slag used in this study was controled by a air-jet method which rapidly cools substance melted at a high temperature. The instant-chilling method prevents from generation of free-CaO in steel slag. The rapidly-chilled steel slag is similar to be spherical shape and has the heavier specific gravity than natural aggregate. Therefore, it could be used to manufacture radiation shielding concrete as fine aggregate. This paper investigated a verity of the engineering properties of radiation shielding concrete when using rapid-chilled steel slag as fine aggregate. As results of this study, the increase of use of rapid-chilled steel slag decreased the desirable water content of concrete but developed the rate of radiation shielding of concrete. The strength of concrete using rapid-chilled steel slag is similar to that of concrete using river sand. In shrinkage, concrete using rapid-chilled steel decreased more than river sand at a long time. Also, to obtain durability of concrete in the freezing and thawing, it is desirable to mix 50% of steel slag in concrete.