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임무현,정낙현 대구대학교 산업기술연구소 1989 産業技術硏究 Vol.8 No.-
Inspite of numorous challenges to produce a new microbial polymer which has similar properties, characteristics and effectiveness for the industry such as natural polymer. There are few of microbial polymer produced as industrial scale and efficiently enough to create volume markets. This article will concentrate largely on the production of poly-β-hydroxybutyrate where some of the most advanced technology has been applied. If the mass production methods and improving technics of properties of PHB will be proceeded, the future for PHB looks exciting provided that they cal fulfil requirements of price and performance.
세포외 다당류 생산을 위한 Zoogloea ramigera 115 균주의 개량
임무현,D. Davidson Easson Jr,Anthony J. Sinskey 대구대학교 산업기술연구소 1989 産業技術硏究 Vol.8 No.-
세포의 다당류의 생산능이 우수한 균주를 Zoogloea ramigera 115로부터 변이 처리하여 선발하였다. Z. ramigera 115균이 Capsule을 형성하여 floc을 만드는데 반하여 이 균은 slime층을 형성하여 용액중에 잘풀리는 다당류를 생산하는 특징을 보여주었으며, Z. ramigera115 SL로 명명하였다. 따라서 이균은 배양적 성질이 모균인 Z. ramigera에 비하여 세포외 다당의 분리가 용이하게 개량되었고 모균에 비하여 약 20%의 생산성의 향상을 나타내었다. 이들 두 균주가 생산하는 다당의 구성분석에서 glucose와 galactose의 구성비가 약 2:1로 모두 동일하게 나타났으며 Z. ramigera 115 SL의 경우 Pyruvate와 D-acetyl group의 함량이 모균주보다 약간 높은 수치를 보였다.
Cacao bean 으로부터 분리된 polyphenol 성분의 화학구조분석과 ACE 저해효과
임무현,이만종,장영렬 한국농화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.1
Seven kinds of polyphenol compounds having ACE activities were isoated and purified by Sephadex LH-20, MCI-gel CHP-20, μ-Bondapak C_(18) and Fuji-gel ODS G₃ sucessively from cacao bean(Ghana). The chemical structures of each compound were determined and identified using analyzers such as ¹H-NMR, ^(13)C-NMR, IR, MS, polarimeter and Elemental Analysis. Inhibition effects of isolated polyphenols on angiotensin converting enzyme (concerned with hypertension) were also observed. The results obtained were as follows,; The compounds isolated and identified were confirmed and determined as compound 1 [(+)-catechin], compound 2 [(-)-epicatechin], compound 3 [procyanidin B-1 : (-)-epicatechin-(4β→8)-(+)catechin], compound 4 [procyanidin B-2 : (-)-epicatechin-(4β→8)-(-)-epicatechin], compound 5 [procyanidin B-7 : (-)-epicatechin-(4β→6)-(+)-catechin], campound 6 (procyanidin B-2,3,3'-O-digallate), compound 7 [cinnamtannin A-2 : (-)-epicatechin-(4β→8)-(-)-epicatechin-(4β→8)-(-)-epicatechin-(4β→8)-(-)-epicatechin]. In the inhibition effect on ACE, procyanidin B-2,3,3'-O-digallate (compound 6) showed a higher value of 94.6% for ACE in 100μM than other compounds such as (+)-catechin (compound 1), (-)-epicatechin (compound 2), procyanidin B-1 (compound 3), procyanidin B-2 (compound 4), procyanidin B-7 (compound 5) and cinnamtannin A-2 (compound 7) showing 67.9%, 61.9%, 88.6%, 82.5%, 72.2% and 82.3% for ACE, respectively. Inhibition of 4β→8 in coupling bond on the ACE enzyme was more effective than that of 4β→6. Procyanidin containing gallate inhibited more effectively than those containing not any. It was also observed that a lot of hydroxy group in the compounds increased the inhibitory effect.
Mortierella nana의 균체지질중의 γ-Linolenic acid 함량에 미치는 비타민의 영향
박준권,임무현 대구대학교 산업기술연구소 2003 産業技術硏究 Vol.14 No.3
Mortiella nana속의 미생물을 이용한 균체지질중의 GLA생산에서 배양배지내 butyric acid, 수용성 및 지용성 비타민류를 첨가하여 이들 미량물질의 인자가 균의 생육, 지질생산 및 균체지질중의 지방산 변화와 GLA 함량변화에 미치는 영향을 검토하였다. Mortiella nana속의 배양에 각종 수용성비타민을 첨가한 결과, thiamin, riboflavin, niacinamide, pyridoxin의 첨가구에서 균체지질과 GLA함량이 높았다. 수용성 비타민중 thiamin의 농도를 달리하여 첨가한 결과 0.5㎍/㎖의 첨가구에서 지질함량 및 GLA 함량이 높게 나타났다. Ribofalvin, niacinamide, pyridoxin을 각각 농도별로 첨가한 경우 균체지질 함량 면에서 대조구보다 높았으나 GLA함량은 대조구보다 낮게 나타나, 균체지질의 생산에는 유리하게 작용하였지만 GLA의 생산에는 영향을 미치지 못하였다. n-alkane계의 butyric acid를 농도별로 첨가후 배양한 결과 05㎍/㎖의 농도의 첨가구에서 지질생산량은 0.43g이었고 GLA생산량에서는 18.5%로 무첨가구보다 2배이상의 생산량을 보여 균체지질 및 GLA생산에 큰 영향을 주는 것으로 나타났다. 지용성 비타민 중 tocopherol과 squalene을 배지에 첨가후 배양한 결과, 모든 농도의 첨가구에서 지질 생산량 및 GLA생산이 무첨가구보다 낮게 나타났다. 배양배지의 초기 pH의 영향을 조사한 결과 pH 6.0의 배양 처리구가 지질함량 0.45g, GLA생산량이 17.95%로 가장 최적의 초기배양 pH였으며 배지의 양과 배양속도를 달리하여 산소공급의 영향을 조사한 결과, 100rpm에서 150㎖의 배지로 배양한 처리구가 균체지질 및 GLA생산량이 높았으며, 배양 6일째 처리구가 균체 지질 및 GLA 생산성이 가장 높게 나타났다. The effects of water soluble and fat soluble vitamins on the content of γ-linolenic acid(GLA) in the mycelial lipid by culture of Mortiella nana, were investigated. Addition of water soluble vitamins, thiamin, riboflavin niacinamide and pyridoxin, in the culture medium of Mortiella nana resulted in the increase of mycelial lipid and GLA content, In the addition of 0.5㎍/㎖ thiamin to the basal medium, mycelial lipid and GLA content were the highest. In the each addition concentration of riboflavin niacimide and pyridoxin, mycelial content were more higher than control, but GLA content were more lower. It was addition of riboflavin niacinamide and pyridoxin found that mycelial lipid production was favorable, but GLA production was not affected. In the addition of 0.5㎍/㎖ butyric acid as the n-alkane to the basal medium containing 0.5㎍/㎖ thiamin, mycelial content was 0.43g/g dry cell weight and It was found 18.5% of GLA production that addition of 0.5㎍/㎖ was more twice higher than blank. It was appeared that addition of butyric acid was affected to mycelial lipid and GLA production. In the addition of fat soluble vitamin, tocopherol and squalene, mycelial lipid and GLA production were lower than blank at all added concentration. At the influence of intial pH of culture medium, pH 6.0 of culture medium was optimum, the following results were 0.45g of mycelium lipid and 17.95% of GLA production. The influence of oxygen applying with the various of medium volume and two shaking speed were also examed, When cultivation was cultured for 6 days with 150㎖ volume of medium at 100rpm, mycelium lipid and GLA production were the highest.