Mortierella nana의 균체지질중의 γ-Linolenic acid 함량에 미치는 비타민의 영향

박준권,임무현 대구대학교 산업기술연구소 2003 産業技術硏究 Vol.14 No.3

Mortiella nana속의 미생물을 이용한 균체지질중의 GLA생산에서 배양배지내 butyric acid, 수용성 및 지용성 비타민류를 첨가하여 이들 미량물질의 인자가 균의 생육, 지질생산 및 균체지질중의 지방산 변화와 GLA 함량변화에 미치는 영향을 검토하였다. Mortiella nana속의 배양에 각종 수용성비타민을 첨가한 결과, thiamin, riboflavin, niacinamide, pyridoxin의 첨가구에서 균체지질과 GLA함량이 높았다. 수용성 비타민중 thiamin의 농도를 달리하여 첨가한 결과 0.5㎍/㎖의 첨가구에서 지질함량 및 GLA 함량이 높게 나타났다. Ribofalvin, niacinamide, pyridoxin을 각각 농도별로 첨가한 경우 균체지질 함량 면에서 대조구보다 높았으나 GLA함량은 대조구보다 낮게 나타나, 균체지질의 생산에는 유리하게 작용하였지만 GLA의 생산에는 영향을 미치지 못하였다. n-alkane계의 butyric acid를 농도별로 첨가후 배양한 결과 05㎍/㎖의 농도의 첨가구에서 지질생산량은 0.43g이었고 GLA생산량에서는 18.5%로 무첨가구보다 2배이상의 생산량을 보여 균체지질 및 GLA생산에 큰 영향을 주는 것으로 나타났다. 지용성 비타민 중 tocopherol과 squalene을 배지에 첨가후 배양한 결과, 모든 농도의 첨가구에서 지질 생산량 및 GLA생산이 무첨가구보다 낮게 나타났다. 배양배지의 초기 pH의 영향을 조사한 결과 pH 6.0의 배양 처리구가 지질함량 0.45g, GLA생산량이 17.95%로 가장 최적의 초기배양 pH였으며 배지의 양과 배양속도를 달리하여 산소공급의 영향을 조사한 결과, 100rpm에서 150㎖의 배지로 배양한 처리구가 균체지질 및 GLA생산량이 높았으며, 배양 6일째 처리구가 균체 지질 및 GLA 생산성이 가장 높게 나타났다. The effects of water soluble and fat soluble vitamins on the content of γ-linolenic acid(GLA) in the mycelial lipid by culture of Mortiella nana, were investigated. Addition of water soluble vitamins, thiamin, riboflavin niacinamide and pyridoxin, in the culture medium of Mortiella nana resulted in the increase of mycelial lipid and GLA content, In the addition of 0.5㎍/㎖ thiamin to the basal medium, mycelial lipid and GLA content were the highest. In the each addition concentration of riboflavin niacimide and pyridoxin, mycelial content were more higher than control, but GLA content were more lower. It was addition of riboflavin niacinamide and pyridoxin found that mycelial lipid production was favorable, but GLA production was not affected. In the addition of 0.5㎍/㎖ butyric acid as the n-alkane to the basal medium containing 0.5㎍/㎖ thiamin, mycelial content was 0.43g/g dry cell weight and It was found 18.5% of GLA production that addition of 0.5㎍/㎖ was more twice higher than blank. It was appeared that addition of butyric acid was affected to mycelial lipid and GLA production. In the addition of fat soluble vitamin, tocopherol and squalene, mycelial lipid and GLA production were lower than blank at all added concentration. At the influence of intial pH of culture medium, pH 6.0 of culture medium was optimum, the following results were 0.45g of mycelium lipid and 17.95% of GLA production. The influence of oxygen applying with the various of medium volume and two shaking speed were also examed, When cultivation was cultured for 6 days with 150㎖ volume of medium at 100rpm, mycelium lipid and GLA production were the highest.

증발하는 액적의 접촉각에 대한 액적 내의 불순물의 영향

박준권,윤진성,이상현 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.5

레벨셋 기법을 이용한 전기습윤 현상의 동적 거동에 대한 해석 및 물성 보간 방법에 대한 고찰

박준권(J.K. Park),강관형(K.H. Kang) 한국전산유체공학회 2010 한국전산유체공학회 학술대회논문집 Vol.2010 No.5

Electrowetting is a versatile tool to handle tiny droplets and forms a backbone of digital microfluidics. Numerical analysis is necessary to fully understand the dynamics of electrowetting, especially in designing electrowetting-based devices, such as liquid lenses and reflective displays. We developed a numerical method to analyze the general contact-line problems, incorporating dynamic contact angle models. The method is based on the conservative level set method to capture the interface of two fluids without loss of mass. We applied the method to the analysis of spreading process of a sessile droplet for step input voltages and oscillation of the droplet for alternating input voltages in electrowetting. The result was compared with experimental data. It is shown that contact line friction significantly affects the contact line motion and the oscillation amplitude. The pinning process of contact line was well represented by including the hysteresis effect in the contact angle models. In level set method, in the mean time, material properties are made to change smoothly across an interface of two materials with different properties by introducing an interpolation or smoothing scheme. So far, the weighted arithmetic mean (WAM) method has been exclusively adopted in level set method, without complete assessment for its validity. We showed here that the weighted harmonic mean (WHM) method for rate constants of various rate processes, including viscosity, thermal conductivity, electrical conductivity, and permittivity, can be an alternative. I.e., the WHM gives more accurate results than the WAM method in certain circumstances. The interpolation scheme should be selected considering various characteristics including type of property, ratio of property of two fluids, geometry of interface, and so on.

Source 공개 코드 OpenFOAM 소개

박준권(J.K. Park),강관형(K.H. Kang) 한국전산유체공학회 2010 한국전산유체공학회 학술대회논문집 Vol.2010 No.5

Recently, several open source codes for computational fluid dynamics (CFD) have been emerged and are spreading fast. Our group has chosen OpenFOAM as a platform to develop our own in-house code. In this paper, we would like to share the information on the codes and what we have experienced so far. We introduce several features of OpenFOAM, which include the performance compared with commercial packages, estimation for current user population, and our own prospect for future improvement in performance and growth in user population. In addition, we briefly introduce our experience gained in embedding the level set method into the OpenFOAM.

레벨셋 기법을 이용한 전기습윤 현상의 동적 거동에 대한 해석

박준권(J.K. Park),홍지우(J.W. Hong),강관형(K.H. Kang) 한국전산유체공학회 2009 한국전산유체공학회 학술대회논문집 Vol.2009 No.11

Electrowetting is a versatile tool to handle tiny droplets and forms a backbone of digital microfluidics. Numerical analysis is necessary to fully understand the dynamics of electro wetting, especially in designing electro wetting-based devices, such as liquid lenses and reflective displays. We developed a numerical method to analyze the general contact-line problems, incorporating dynamic contact angle models. The method is based on the conservative level set method to capture the interface of two fluids without loss of mass. We applied the method to the analysis of spreading process of a sessile droplet for step input voltages and oscillation of the droplet for alternating input voltages in electro wetting. The result was compared with experimental data. It is shown that contact line fiction significantly affects the contact line motion and the oscillation amplitude. The pinning process of contact line was well represented by including the hysteresis effect in the contact angle models.

Source 공개 코드 OpenFOAM에 대한 리뷰

박준권(J.K. Park),강관형(K.H. Kang) 한국전산유체공학회 2010 한국전산유체공학회지 Vol.15 No.3

Recently, several open source codes for computational fluid dynamics (CFD) have been introduced and are spreading fast. Our group has chosen the OpenFOAM as a platform to develop our own in-house code. In this brief review, we would like to share the information on the codes and what we have experienced so for. We introduce several features of OpenFOAM, which include the performance compared with commercial packages, estimation for current user population, and our own prospect for future improvement in performance and growth in user population. In addition, we briefly introduce our experience gained in embedding the level set method into the OpenFOAM.

레벨셋 기법을 이용한 전기습윤 현상의 동적 접촉각 문제에 대한 수치해석

박준권(J.K. Park),강관형(K.H. Kang) 한국전산유체공학회 2009 한국전산유체공학회 학술대회논문집 Vol.2009 No.4

We developed a numerical method to analyze the contact-line problems, incorporating a dynamic contact angle model. We used level set method to capture free surface. The method is applied to the analysis of dynamic behavior of a droplet in DC electro wetting. The result is compared with an experimental data and result of perturbation method.

전기습윤 현상에 대한 수치해석적 연구

박준권(Jun Kwon Park),강관형(Kwan Hyoung Kang) 한국가시화정보학회 2009 한국가시화정보학회 학술발표대회 논문집 Vol.2009 No.11

Electrowetting is a flexible method to control the wetting behavior of tiny droplets and forms a backbone of digital microfluidics. Numerical analysis is necessary to fully understand the dynamics of electrowetting, especially in designing electrowetting-based devices, such as liquid lenses and reflective displays. We numerically analyzed spreading process of a sessile droplet for step input voltages and oscillation of the droplet for alternating input voltages in electrowetting. We investigated the effect of contact line friction and contact line hysteresis on the dynamic behavior of the droplet. The results were compared with experimental data.

레벨셋 기법을 이용한 액적 충돌 현상에 대한 해석 : 동적 접촉각 모델의 영향

박준권(Jun Kwon Park),홍지우(Jiwoo Hong),강관형(Kwan Hyoung Kang) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.5

Attraction between Like-Charged Droplets

Jun Kwon Park(박준권),Sung Hee Ko(고성희),Kwan Hyoung Kang(강관형) 대한기계학회 2007 대한기계학회 춘추학술대회 Vol.2007 No.10

Mysterious attractive motions between like-charged droplets on a solid surface are observed in electrospray. Although droplets are charged with same sign of charge, the droplets pull each other and coalesce. An explanation for this phenomenon is suggested. A numerical analysis is carried out to investigate the electrostatic interaction of two like-charged droplets. It is shown that like-charged droplets attract or repel each other depending on the electric potential of each droplet.