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DIRECT GROWTH OF MULTI-WALLED CARBON NANOTUBES ON SHARP TIPS FOR ELECTRON MICROSCOPY
M. MANN,K. B. K. TEO,W. I. MILNE,T. TESSNER 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2006 NANO Vol.1 No.1
The favorable electron optical properties of carbon nanotubes (CNTs) have been studied in detail, but the application to electron sources has been limited by the complexity of the fabrication process. We report the use of Plasma Enhanced Chemical Vapor Deposition (PECVD) for the direct deposition of multi-walled CNTs onto the apex of sharply etched tungsten tips, aligned to the vertical axis of the tips. We show that these emitters have excellent stability.
Anna M. Davidson,David Da Silva,Sebastian Saa,Phillip Mann,Theodore M. DeJong 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.4
The plastochron, defined as the time interval between the initiations of two successive leaves, can also indicate the development of successive phytomers along a shoot. Previous work has shown that crop load impactsthe plastochron in field-grown peach (Prunuspersica) trees, which led us to hypothesize that the plastochron of peach trees may be sensitive to the carbon status of the tree. To testthis hypothesis, a 38-day growth chamber study was conducted to determine if elevated CO2 (800 μmol·mol-1) speeds up the plastochron of young peach trees relative to their growth in ambient (400 μmol·mol-1) CO2. The leaf lamina lengths were measured every other day to generate leaf growth rate curves that were fitted against a classic Gompertz growth curve to estimate the time of the initiation of each leaf, which in turn, was used to estimate the plastochron. Additionally, in order to non-destructively gauge the effects of CO2 concentration on plant performance during the experiment, net leaf CO2 assimilation and stomatal conductance measurements were taken approximately half way through and at the end of the 38-day experiment. Doubling the ambient CO2 concentration had no effect on the plastochron, even though the leaf CO2 assimilation rates, leaf starch and total nonstructural carbohydrate concentrations were greater in trees grown in elevated CO2. In addition, there were no significant treatment differences in incremental shoot growth or the number of lateral syleptic shoots.
Park, K.,Kim, Y.I.,Shin, K.O.,Seo, H.S.,Kim, J.Y.,Mann, T.,Oda, Y.,Lee, Y.M.,Holleran, W.M.,Elias, P.M.,Uchida, Y. Butterworths ; Elsevier Science Ltd 2014 The Journal of nutritional biochemistry Vol.25 No.7
We recently discovered that a signaling lipid, sphingosine-1-phosphate (S1P), generated by sphingosine kinase 1, regulates a major epidermal antimicrobial peptide's [cathelicidin antimicrobial peptide (CAMP)] expression via an NF-κB→C/EBPα-dependent pathway, independent of vitamin D receptor (VDR) in epithelial cells. Activation of estrogen receptors (ERs) by either estrogens or phytoestrogens also is known to stimulate S1P production, but it is unknown whether ER activation increases CAMP production. We investigated whether a phytoestrogen, genistein, simulates CAMP expression in keratinocytes, a model of epithelial cells, by either a S1P-dependent mechanism(s) or the alternate VDR-regulated pathway. Exogenous genistein, as well as an ER-β ligand, WAY-200070, increased CAMP mRNA and protein expression in cultured human keratinocytes, while ER-β antagonist, ICI182780, attenuated the expected genistein- and WAY-200070-induced increase in CAMP mRNA/protein expression. Genistein treatment increased acidic and alkaline ceramidase expression and cellular S1P levels in parallel with increased S1P lyase inhibition, accounting for increased CAMP production. In contrast, siRNA against VDR did not alter genistein-mediated up-regulation of CAMP. Taken together, genistein induces CAMP production via an ER-β→S1P→NF-κB→C/EBPα- rather than a VDR-dependent mechanism, illuminating a new role for estrogens in the regulation of epithelial innate immunity and pointing to potential additional benefits of dietary genistein in enhancing cutaneous antimicrobial defense.
Kevin M. Rice,Siva K. Nalabotu,Nandini D.P.K. Manne,Madhukar B. Kolli,Geeta Nandyala,Ravikumar Arvapalli,Jane Y. Ma,Eric R. Blough 대한예방의학회 2015 예방의학회지 Vol.48 No.3
Objectives: With recent advances in nanoparticle manufacturing and applications, potential exposure to nanoparticles in various settings is becoming increasing likely. No investigation has yet been performed to assess whether respiratory tract exposure to cerium oxide (CeO2) nanoparticles is associated with alterations in protein signaling, inflammation, and apoptosis in rat lungs. Methods: Specific-pathogen-free male Sprague-Dawley rats were instilled with either vehicle (saline) or CeO2 nanoparticles at a dosage of 7.0 mg/kg and euthanized 1, 3, 14, 28, 56, or 90 days after exposure. Lung tissues were collected and evaluated for the expression of proteins associated with inflammation and cellular apoptosis. Results: No change in lung weight was detected over the course of the study; however, cerium accumulation in the lungs, gross histological changes, an increased Bax to Bcl-2 ratio, elevated cleaved caspase-3 protein levels, increased phosphorylation of p38 MAPK, and diminished phosphorylation of ERK-1/2-MAPK were detected after CeO2 instillation (p<0.05). Conclusions: Taken together, these data suggest that high-dose respiratory exposure to CeO2 nanoparticles is associated with lung inflammation, the activation of signaling protein kinases, and cellular apoptosis, which may be indicative of a long-term localized inflammatory response.
All-oral Dual Therapy with Daclatasvir and Asunaprevir in Patients with HCV Genotype 1B Infection
Michael Manns,Stanislas Pol,Ira M. Jacobson,Patrick Marcellin,Stuart C. Gordon,Cheng-Yuan Peng,Ting-Tsung Chang,Gregory T. Everson,Jeong Heo,Guido Gerken,Boris Yoffe,William J. Towner,Marc Bourliere,S 한국간담췌외과학회 2014 한국간담췌외과학회 학술대회지 Vol.2014 No.4
Rice, Kevin M.,Nalabotu, Siva K.,Manne, Nandini D.P.K.,Kolli, Madhukar B.,Nandyala, Geeta,Arvapalli, Ravikumar,Ma, Jane Y.,Blough, Eric R. The Korean Society for Preventive Medicine 2015 예방의학회지 Vol.48 No.3
Objectives: With recent advances in nanoparticle manufacturing and applications, potential exposure to nanoparticles in various settings is becoming increasing likely. No investigation has yet been performed to assess whether respiratory tract exposure to cerium oxide ($CeO_2$) nanoparticles is associated with alterations in protein signaling, inflammation, and apoptosis in rat lungs. Methods: Specific-pathogen-free male Sprague-Dawley rats were instilled with either vehicle (saline) or $CeO_2$ nanoparticles at a dosage of 7.0 mg/kg and euthanized 1, 3, 14, 28, 56, or 90 days after exposure. Lung tissues were collected and evaluated for the expression of proteins associated with inflammation and cellular apoptosis. Results: No change in lung weight was detected over the course of the study; however, cerium accumulation in the lungs, gross histological changes, an increased Bax to Bcl-2 ratio, elevated cleaved caspase-3 protein levels, increased phosphorylation of p38 MAPK, and diminished phosphorylation of ERK-1/2-MAPK were detected after $CeO_2$ instillation (p<0.05). Conclusions: Taken together, these data suggest that high-dose respiratory exposure to $CeO_2$ nanoparticles is associated with lung inflammation, the activation of signaling protein kinases, and cellular apoptosis, which may be indicative of a long-term localized inflammatory response.
Le, Duy,Nguyen, Chon M.,Mann, Richard K.,Yerkes, Carla N.,Kumar, Bobba V.N. The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.44 No.4
Barnyardgrass (Echinochloa crus-galli) is one of the worst weeds in rice (Oryza sativa), but there are few reports about the genetic diversity and herbicide resistance of barnyardgrass in Vietnam. In this study, we used random amplified polymorphic DNA (RAPD) analysis and greenhouse testing to study the genetic diversity and quinclorac resistance levels of 15 Echinochloa crus-galli populations in the Mekong Delta, Vietnam, and the state of Arkansas, U.S. The quinclorac resistance of Echinochloa crus-galli populations in Vietnam was confirmed; 9 populations were resistant to quinclorac with R/S ratios ranging from 1.9 to 6.3. Six oligonucleotide primers produced a total of 55 repeatable bands of which 46 were polymorphic (83.3% average) among the 15 populations. Genetic distance was calculated, and cluster analysis separated the 15 populations into 2 main clusters with the genetic distances within the clusters ranging from 0.09 to 0.39. The two main clusters were divided into 7 subclusters, and the quinclorac resistant and susceptible populations were located randomly within each subcluster. Six out of 13 weed populations from Vietnam belonged to one cluster and a single Echinochloa species. The remaining 7 populations were identified as potentially different species in the Echinochloa genus. Nine Echinochloa populations from Vietnam were tested and identified as quinclorac resistant. The connection between quinclorac resistance levels and weed groups defined by RAPD analysis in the study is unclear; the quinclorac resistance of each resistant population could have evolved individually, regardless of differences in genetic diversity and location of the sampled populations.
간세포암과 간경변증의 감별 진단에 있어서 Lens culinaris agglutinin-A 에 반응하는 AFP 분획 측정의 임상적 의의
송근암(Geum A m Song),김태오(Tae Oh Kim),송철수(Chul Soo Song),조몽(Mong Cho),양웅석(Ung Suk Yang),류수형(Soo Hyung Ryu),이성훈(Seong Hwun Lee),조병만(Byung Mann Cho) 대한내과학회 1999 대한내과학회지 Vol.57 No.5
N/A AFP has been the most useful tumor marker of hepatocellular carcinoma (HCC) because AFP is tumor specific and organ specific. However it can be elevated in liver cirrhosis (LC) and chronic hepatitis as well. AFP in hepatocellular carcinoma has been known to be highly reactive Lens Culinaris Agglutinin-A (LCA-A). The aim of our study is to find whether LCA-A reactive AFP (AFP-L3) is useful in differentiation of HCC and LC with high serum AFP level (>20 ng/ml). Methods : The material consists of sera from 18 patients with HCC and 16 patients with LC whose serum AFP concentration was above 20 ng/ml. Sera were tested for AFP-L3 by lectin-affinity electrophoresis, coupled with antibody affinity blotting with alpha-fetoprotein differentiation kit L (Wako Pure Chemical Industries, Osaka, Japan). AFP-L3 fraction percentage was checked by densitometry (580nm, Cliniscan II, Helena). Results : AFP-L3 detection rate in membrane was 61.1% in HCC and 37.5% in LC. When cut-off value level is 15%, the positive rate was 61.1% in HCC and 31.2% in LC, and sensitivity and specificity of AFP-L3 fraction in HCC was 61.1% and 68.8% respectively. In according to these results, AFP-L3 was a useful marker in differentiation HCC and LC with high serum AFP level. There was no significant relation in AFP-L3 fraction and tumor size, but there was significant relation between AFP-L3 fraction and total AFP serum level(P=0.049). Conclusion : These results lead us to conclude that the level of AFP-L3 is a useful marker in differentiation of HCC and LC with high serum AFP level. (Korean. J. Med 57:875-880, 1999)