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Identification and characterization of presence/absence variation in maize genotype Mo17
Lu Jiang,Yuanda Lv,Tan Li,Han Zhao,Tifu Zhang 한국유전학회 2015 Genes & Genomics Vol.37 No.6
Presence/absence variation (PAV), a major class of genome structure variation, is pervasive in the maize genome. PAVs present in the B73 but absent from the Mo17 genome have been reported in previous studies. Here, the next-generation sequencing was used to identify the PAVs present in Mo17 but absent from B73. A total of 119 PAVs were identified, of which 57 were validated by PCR. Using the intermated B73 9 Mo17 segregating population, 57 validated PAVs were mapped into the genetic map. These PAVs were dispersed on the ten chromosomes. Also, several large genetic regions of PAVs were identified. This suggested the possibility that large genetic fragments in Mo17 were absent from the B73 genome. Several PAVs were also found to be related to disease resistance suggesting that presence/absence variations in the genome may play a role in disease resistances in maize. In addition, the majority of the genes within PAV were transcriptionally silenced or expressed at low levels in some tissues, especially for PAVs related to disease resistance. The results of this study not only provided the identities of additional PAVs in maize but also helped understand the functional role of PAVs in this agriculturally important crop.
Jiang Wan-Zhu,Yao Fang-Jie,Lu Li-Xin,Fang Ming,Wang Peng,Zhang You-Min,Meng Jing-Jing,Lu Jia,Ma Xiao-Xu,He Qi,Shao Kai-Sheng 한국미생물학회 2021 The journal of microbiology Vol.59 No.1
Gloeostereum incarnatum is an edible medicinal mushroom widely grown in China. Using the whole genome of G. incarnatum, simple sequence repeat (SSR) markers were developed and synthetic primers were designed to construct its first genetic linkage map. The 1,048.6 cm map is composed of 10 linkage groups and contains 183 SSR markers. In total, 112 genome assembly sequences were anchored, representing 16.43 Mb and covering 46.41% of the genome. Selfing populations were used for quantitative trait loci (QTL) targeting, and the composite interval mapping method was used to co-localize the mycelium growth rate (potato dextrose agar and sawdust), growth period, yield and fruiting body length, and width and thickness. The 14 QTLs of agronomic traits had LOD values of 3.20–6.51 and contribution rates of 2.22– 13.18%. No linkage relationship was found between the mycelium growth rate and the growth period, but a linkage relationship was observed among the length, width and thickness of the fruiting bodies. Using NCBI’s BLAST alignment, the genomic sequences corresponding to the QTL regions were compared, and a TPR-like protein candidate gene was selected. Using whole-genome data, 138 candidate genes were found in four sequence fragments of two SSR markers located in the same scaffold. The genetic map and QTLs established in this study will aid in developing selective markers for agronomic traits and identifying corresponding genes, thereby providing a scientific basis for the further gene mapping of quantitative traits and the marker-assisted selection of functional genes in G. incarnatum breeding programs.
Current Status of Etiology, Epidemiology, Clinical Manifestations and Imagings for COVID-19
Jiang Meng Di,Zu Zi Yue,Schoepf U. Joseph,Savage Rock H.,Zhang Xiao Lei,Lu Guang Ming,Zhang Long Jiang 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.10
Coronavirus disease 2019 (COVID-19) is a transmissible respiratory disease that was initially reported in Wuhan, China in December 2019. With the alarming levels of COVID-19 spread worldwide, the World Health Organization characterized COVID-19 as a pandemic. Over the past several months, chest CT has played a vital role in early identification, disease severity assessment, and dynamic disease course monitoring of COVID-19. The published data has enriched our knowledge on the etiology, epidemiology, clinical manifestations, and pathologic findings of COVID-19. Additionally, as the imaging spectrum of the disease continues to be defined, extrapulmonary infections or other complications will require further attention. This review aims to provide an updated framework and essential knowledge with which radiologists can better understand COVID-19.
Neuropeptide Y Promotes the Treatment of Adipose Stem Cells on Type 2 Diabetic Wounds
Lu Tinghuan,Cong Lu,Jiang Tong,Dong Xiao,Song Lili 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.5
BACKGROUND: Type 2 diabetes (T2D) is a common metabolic disorder. Due to insufficient insulin secretion or insulin resistance, increased blood glucose often leads to impaired wound healing in T2D patients. Our previous research showed that adipose-derived stem cells (ASCs) from normal mice and T2D mice improved the cutaneous wound healing of diabetic mice. We also found that the expression of neuropeptide Y (NPY) in T2D ASCs was significantly decreased. METHODS: In order to explore the effects of NPY on ASCs and diabetic wound healing, we investigated the effects of NPY on ASCs proliferation and growth factors expression and secretion, the effects of NPY on skin fibroblasts, and the effects of NPY combined with ASCs on T2D wound healing. RESULTS: The results showed that a certain concentration of NPY could promote the proliferation and the growth factors expression and secretion of ASCs, and promote the proliferation and migration of fibroblasts. At the same time, NPY and ASCs have a synergistic effect, which can promote wound healing and decrease inflammation in T2D wounds. NPY may regulate ASCs through the ERK pathway. These results are conducive to promoting ASCs and NPY in the treatment of diabetic wounds. CONCLUSIONS: NPY can promote the effect of ASCs in the treatment of diabetic wounds.
Lu-Lu Zhang,Yi-Yang Li,Jiang Hu,Guan-Qun Zhou,Lei Chen,Wen-Fei Li,Ai-Hua Lin,Jun Ma,Zhen-Yu Qi,Ying Sun 대한암학회 2018 Cancer Research and Treatment Vol.50 No.4
Purpose Local relapse-free survival (LRFS) differs widely among patients with T4 category nasopharyngeal carcinoma (NPC). We aimed to build a nomogram incorporating clinicopathological information to predict LRFS in T4 NPC after definitive intensity-modulated radiation therapy (IMRT). Materials and Methods Retrospective study of 415 Chinese patients with non-metastatic T4 NPC treated with definitive IMRT with or without chemotherapy at our cancer center between October 2009 and September 2013. The nomogram for LRFS at 3 and 5 years was generated based on multivariate Cox proportional hazards regression, and validated using bootstrap resampling, assessing discriminative performance using the concordance index (C-index) and determining calibration ability via calibration curves. Results Five-year LRFS was 88.8%. We identified and incorporated four independent prognostic factors for LRFS: ethmoid sinus invasion, primary gross tumor volume, age, and pretreatment body mass index. The C-index of the nomogram for local recurrence was 0.732 (95% confidence interval, 0.726 to 0.738), indicating excellent predictive accuracy. The calibration curve revealed excellent agreement between nomogram-predicted and observed LRFS probabilities. Risk subgroups based on total point score cutoff values enabled effective discrimination of LRFS. Conclusion This pretreatment nomogram enables clinicians to accurately predict LRFS in T4 NPC after definitive IMRT, and could help to facilitate personalized patient counselling and treatment strategies.
Research on anti-seismic property of new end plate bolt connections - Wave web girder-column joint
Haotian Jiang,Qingning Li,Lei Yan,Chun Han,Wei Lu,Weishan Jiang 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.22 No.1
The domestic and foreign scholars conducted many studies on mechanical properties of wave web steel beam and high-strength spiral stirrups confined concrete columns. Based on the previous research work, studies were conducted on the anti-seismic property of the end plate bolt connected wave web steel beam and high-strength spiral stirrups confined concrete column nodes applied with pre-tightening force. Four full-size node test models in two groups were designed for low-cycle repeated loading quasi-static test. Through observation of the stress, distortion, failure process and failure mode of node models, analysis was made on its load-carrying capacity, deformation performance and energy dissipation capacity, and the reliability of the new node was verified. The results showed that: under action of the beam-end stiffener, the plastic hinges on the end of wave web steel beam are displaced outward and played its role of energy dissipation capacity. The study results provided reliable theoretical basis for the engineering application of the new types of nodes.
Removal characteristics of Cd(II) ions from aqueous solution on ordered mesoporous carbon
Linhang Lu,Haibo Zhao,Lu Yan,Guowei Wang,Yulin Mao,Xin Wang,Kai Liu,Xiufang Liu,Qian Zhao,Tingshun Jiang 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.10
Ordered mesoporous carbon (CMK-3) was synthesized using SBA-15 mesoporous molecular sieve as a template and sucrose as carbon source. The materials were characterized by XRD, TEM and N2 physical adsorption technique. The resulting CMK-3 was used as adsorbent to remove Cd(II) ions from aqueous solution. The effect of pH, contact time and temperature on adsorption process was investigated in batch experiments. The results showed that the removal percentage could reach ca. 90% at the conditions of initial Cd(II) ions concentration of 20 mg/L, dose of 20mg, pH 6.5, contact time of 3h and 293K. Langmuir and Freundlich models were employed to describe the adsorption equilibrium. The kinetics data were described by the pseudo-first-order and pseudo-second-order models, respectively. The adsorption isotherm was well fitted to the Langmuir model, and the adsorption process was well described by the pseudo-second-order kinetic model.