Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

초등교사의 열정에 대한 인식: 의미연결망 분석의 활용

임효진 ( Hyo Jin¸ Lim ),최은혜 ( Eun Hye¸ Choi ),박호익 ( Ho Ik¸ Park ),이지현 ( Ji Hyun¸ Lee ) 한국초등교육학회 2021 초등교육연구 Vol.34 No.3

본 연구에서는 초등교사가 가지고 있는 열정에 대한 인식구조를 파악하기 위해 현직 초등교사 131명을 대상으로 설문조사를 실시하였다, 연구에서는 열정, 열정의 장단점, 교사열정에 대한 인식구조를 알아보기 위해 의미연결망 분석을 활용하여 근접 중심성, 연결중심성, 매개중심성, 아이겐벡터 중심성을 위주로 살펴보았다. 연구결과 첫째, 열정에서는 ‘힘’,‘강렬’과 같은 높은 강도나 에너지를 나타내는 단어들이 중요한 역할을 하였으며 커뮤니티 분석 결과 열정의 행동, 정서, 동기 특성에 대한 주제가 분류되었다. 둘째, 열정의 장점에서는 ‘삶’,‘원동력’,‘에너지’등이, 열정의 단점에서는 ‘타인’,‘피로’,‘살피지 못함’등이 중요한 역할을 하는 것으로 보아 기존 이론에 나타난 열정의 특성을 설명하고 있었다. 셋째, 교사열정에서는 ‘학생’,‘수업’과 같은 교수활동의 실천을 나타내는 단어들이 중요한 역할을 하였으며 커뮤니티 분석 결과 교육활동 전반, 학생과 수업, 전문성 개발에 대한 주제가 분류되었다. The purpose of this study was to explore the meaning of passion perceived by elementary school teachers. In order to understand the structure of perception of passion, we surveyed 131 in-service elementary school teachers. Responses to passion, the positive and negative role of passion, and teacher passion were analyzed using a semantic network analysis. We focused on closeness centrality, degree centrality, betweenness centrality and eigenvector centrality. Results showed that first, in passion, words indicating high strength and energy such as‘power’and‘intensity’played an important role in the structure of perception. Themes on behavioral, emotional, and motivational characteristics of passion were classified as sub-factors of passion as a result of community analysis. Secondly,‘life’,‘motivation’, and‘energy’appeared in key-word analysis in the positive role of passion while‘others’,‘fatigue’, and‘not caring for’appeared in the negative role of passion, reflecting the theory on the dualistic model of harmonious and obsessive passion. Third, in teacher passion, words indicating teaching activities and their practices such as‘student’and‘instruction’played an important role in the structure of perception. Themes related to educational activities in general, students and instruction, and professional development were classified as sub-factors of teacher passion.

농흉과 종격동염이 합병된 viridans streptococcus에 의한 인후농양 1예

임상현,유진홍,권순석,김연식,신완식,강문원,정명희 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.2

최근 저자들은 viridans streptococcus에 의한 인후농약으로 인하여 발생한 종격동염 및 농흉을 보인 47세 여자 환자를 적절한 배농과 항생제로 치료한 국내 최초의 1예를 치험하였기에 문헌 고찰과 함께 보고하는 바이다. Since the antibiotic era, retropharyngeal abscess became a rare disease entity nowadays. But it may often progress to lethal complications involving vital structure if diagnosis or treatment is delayed. We experienced a case of empyema and mediastinitis complicating retropharyngeal abscess caused by viridans streptococcus in a 47-year-old female patient with diabetes mellitus. She was successfully treated with appropriate antibiotic therapy combined with percutaneous abscess drainage. To our knowledge, such a complication of retropharyngeal abscess has not been reported in Korea yet and we present this unusual case herein followed by a review of the related literature.

심정지환자에서 epinephrine투여시 혈중 catecholamine의 변화

임경수,황성오,이부수,이강현,이진웅,임종천,김영식,김선만,김 현 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Background : Cardiac arrest is a potent stimulus for the release of endogenous catecholamine and high plasma catecholamine concentration has been reported during cardiopulmonary resuscitation(CPR). Few data were present about the clinical effect of high plasma catecholamine and endogenous catecholamine response to exogenous epinephrine administration during CPR. Purpose : This study was designed to evaluate endogenous catecholamine response to cardiac arrest and exogenously administered epinephrine during CPR. Method : In 21 cardiac arrest victims, blood sample for epinephrine and norepinephrine was drawn before and 1 minute after each epinephrine administration during CPR. 1mg of epinephrine was administered immediately after intravenous access and 3 minute interval during CPR. Plasma catecholamines were quantitated by high-performance liquid chromatography. Result : Baseline plasma epinephrine concentraqtion was higher in patients with cardiac arrest than normal controls (985±1627 vs 194±173 pg/ml, p<0.05). Baseline plasma norepinephrine concentration was also elevated in patients with cardiac arrest than normal controls, although statistically insignificnat(1965±4915 vs 360±250 pg/ml). Plasma epinephrine concentration in cardiac arrest patients was significantly elevated after exogenous administration of epinephrine, but its response was blunted after the third does of epinephrine. Plasma norepinephrine concentration in cardiac arrest patients was also elevated, but its response was observed only after the first epinephrine administration. Plasma epinephrine concentration was persistently higher in patients with return of spontaneous circulation (ROSC) than patients without ROSC. there was no significant difference of plasma norepinephrine concentration whether spontaneous circulation was restored or not. Endogenous epinephrine response to exogenous epinephrine was not observed in patients having prolonged arrest time over 20 minutes. Conclusion : Results of this study suggests that significant amount of catecholamine is released by exogenous epinephrine administration in spite of high plasma catecholamine concentration, and degree of endogenous catecholamine response influences resuscitation outcome.

기기 중성자방사화분석법을 이용한 황사특성

임종명,이현석,이진홍 忠南大學校 環境問題硏究所 2002 環境硏究 Vol.20 No.-

Using INAA, which has been thought to be one of the best analytical techniques for airborne particulate matters, this study centered on the quantitative analysis of 32 metal elements within Asian Dust aerosols. Concentrations of REEs of crustal origin, such as Ce, Cs, Dy, La, Sc, and Sm were more than 8.5 times higher in Asian Dust aerosols. In addition, major crustal elements, such as Al, Ca, Co, Fe, K, Mg, Mn, and Ti showed more than 3 times higher concentrations in those aerosols, which made PM 10 and all metal concentrations be also about 3.0 and 4.8 times higher, respectively. However, concentrations of anthropogenic origin, such as As, Br, Ci, I, In, Sb, Se, and Zn were increased to be a little higher, which means no great effect of Asian Dust on these elements.

RAW 264.7 세포에 대한 중국산 천연 광물성 섬유 TAFMAG의 독성효과

임영,한진구,김지홍,김현욱,김은경,김경아,장황신 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.3

Objectives : This study was designed to evaluate cytotoxicity of TAFMAG, which is a trade name of natural mineral fiber mined and produced in China. Methods : The cytotoxicity of TAFMAG was evaluated by measuring iron content, lipid peroxidation, erythrocyte hemolysis, and cytotoxicity in vitro. These results were compared with the data of chrystotile and wollastonite as a positive and negative control, respectively. Results : There was significant increase of Fenton activity in TAFMAG and chrysotile with dose-response pattern. The iron chelating agent, desferrioxamine, significantly decreased Fenton activity of the particulates except wollastonite. TAFMAG and chrysotile fibers significantly increased malondialdehyde concentration from lipid peroxidation of the red blood cell membrane. In erythrocyte hemolysis test, TAFMAG & chrysotile had stronger effect on erythrocyte hemolysis than wollastonite with the concentration of 1,000g/ml. Furthermore, TAFMAG was more hemolytic than chrysotile with the concentration of 5,000g/ml. There was a significant cytotoxic effect in TAFMAG and chrysotile on RAW cell compared with wollastonite. Conclusions : In vitro study suggested that TAFMAG may have a similar health hazard as usual asbestos.

인터넷을 활용한 진로정보탐색 프로그램이 대학생의 진로 자기효능감에 미치는 영향

임은미,김현진 전주대학교 사회과학종합연구소 2002 社會科學論叢 Vol.18 No.-

본 연구는 인터넷을 활용한 진로정보탐색 프로그램이 대학생들의 진로자기효능감에 미치는 영향을 검증하기 위한 것이다. 이에 대학생을 대상으로 하는 인터넷을 활용한 진로정보탐색 프로그램을 제작하였다. 이 프로그램은 김봉환 등(2001)이 제안한 모듈식 진로상담 프로그램의 개념을 임은미와 장선숙(2002)이 중등학생을 위해 적용한 것을 대학생의 상황에 맞게 수정하여 제작되었다. 프로그램 회기는 4회기로 구성되어 있으며, 그 내용은 프로그램 분위기 조성 및 온라인 직업흥미검사 실시(60분), 직업흥미검사 결과의 해석과 직업세계의 이해(60분), 정보사냥대회(60분), 직업정보탐색과 마무리로 이루어져 있다. 프로그램의 실시 전후에 <진로의사결정 자기효능감 검사>를 실시하고, 사전-사후검사 점수 차이를 분석하여 프로그램의 효과를 검증하였다. J대학교의 1학년 남녀학생 가운데 자발적인 신청자들을 대상으로 본 프로그램을 적용한 후 실험집단과 통제집단의 사후검사 점수 차이를 비교한 결과 프로그램 참가자들은 통제집단에 비하여 사후검사에서 의미있게 높은 점수를 얻었다. 이러한 경향은 진로 의사결정 전체 Q뿐 아니라 문제해결 하위영역을 제외한 목표설정, 계획수립, 자기평가, 직업정보탐색의 전 하위 영역에서도 동일하게 나타났다. 이러한 결과는 대학생을 대상으로 인터넷을 활용하여 단기에 실시하는 진로정보탐색 프로그램이 대학생의 진로의사결정 효능감을 높여줄 수 있음을 시사한다. The purpose of this study is to examine the effects of career information exploration program using internet on the undergraduates' career decision-making self-efficacy. For this purpose, career information exploration program using internet was developed. The main factors of this program are performing the free on-line interest test, explorating the career information web-sites, and getting career information through internet according to the participants' interests. The results showed that the experimental group got higher score than the control group in CDMSES(Career Decision-Making Self-Efficacy Inventory). But for the problem solving subcategory, this tendency was shown in all the sub factors of CDMSES, i.e. goal-setting, career planning, collecting information, and self -evaluation. The implications about education or counseling programs for the undergrauates were discussed.

Sr·페라이트의 자기특성에 관한 연구

임재근,서강수,문현욱,신용진 明知大學校 産業技術硏究所 1992 産業技術硏究所論文集 Vol.11 No.-

This thesis is the study on the magnetic properties of strontium (Sr) ferrite. Specimens of SrO·nFe₂O₃,basic composition, with n=5.9 which is nonstoichiomatric composition region were prepared by sintering at 1275°C for 1hr, whthin air surroundings. In order to increase coercivity, additives of 0.4wt%SiO₂ and 0.7wt%CaCO₃ were added into basic composition, and 0.5wt%∼2.0wt%Cr₂O₃ was added as second additives. In case of second additives 1.5wt%Cr₂O₃, it was obtained uniformed grain size and magnetic properties was increased. The sintering temperature was 1150°C, 1180°C, 1210°C, 1240°C and 1270°C respectivily and cooling was performed in furnance. From the research and experiments on the magnetic properties of strontium (Sr) ferrite based on the complex additive technique, it was obtained good magnetic properties of (BH)mas=0.72MGOe, at the sintering temperature of 1210°C, in the case of (basic composition)+(basic additive)+(1.5wt%Cr₂O₃)

외상후 혈뇨환자에서 응급 초음파검사의 의의

임경수,이강현,황성오,이진웅 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

The Ultrasonography is rapid, mobile, and noninvasive diagnostic tool in emergency center. As a result, we estimate it`s value as an advocated primary diagnostic modality in the immediate evaluation of patients with multiple trauma. We investigated 60 patients with traumatic hematuria(microscopic hematuria or gross hematuria) who visited our emergency department from 1995, July to 1996, March. To evaluate the injury of urinary systems, emergency physician examined patients with hematuria by ultrasonography, and comapared the results with those of excretory urography and computed tomography. We demonstrated 21 positive findings for abnormal fluid collection and renal parenchymal injury and bladder rupture on ultrasonography. All bladder injuries were diagnosised by ultrasonography, and all major renal injury(grade Ⅲ,Ⅳ,Ⅴ) were also diagnosised by that. The accuracy of emergency ultrasound examination in traumatic hematuria was 97.0%, and sensitivity was 95.0% with specificity of 97.4%. The ultrasonography was an accurate diagnostic adjunct in detecting renal injury and bladder injury in traumatic hematuria patients. Ultrasonography provides an excellent noninvasive diagnostic tool for the initial screening study of patients with traumatic hematuria.

대전 3, 4 공단지역의 대기중 중금속의 농도

임종명,이진홍,윤미정,장미숙,남병현 忠南大學校 環境問題硏究所 1999 環境硏究 Vol.17 No.-

This research centered on seasonal analysis for the concentration of heavy metals in 5 sites within Taejon 3rd and 4th industrial Complexes. Total suspended particulates were collected on glass microfibre filters (Whatman EPM 2000) by high volume air samplers. About 20 metal elements including 13 toxic ones were analyzed by ICP-MS and ICP-AES after the pretreatment of mixed acid extraction. The following results were summarized from the research : 1) the concentrations of TSPs were 17.7∼219.6㎍/㎥ while the arithmetic mean concentration was 101.7㎍/㎥ ; 2) the arithmetic mean concentration of human carcinogen such as arsenic, hexavalent chromium and nickel subsulfide was 9.99, 6.68 and 6.13 ng/㎥ while that of probable human carcinogen such as beryllium, cadmium and lead was 0.12, 3.37, 97.65 ng/㎥, respectively.