Mechanical behavior of steel tube encased high-strength concrete composite walls under constant axial load and cyclically increasing lateral load: Experimental investigation and modeling

Liang Bai,Huilin Wei,Bin Wang,Fangfang Liao,Tianhua Zhou,Xing-Wen Liang 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.47 No.1

This paper presented an investigation into steel tubes encased high-strength concrete (STHC) composite walls, wherein steel tubes were embedded at the boundary elements of high-strength concrete walls. A series of cyclic loading tests was conducted to evaluate the failure pattern, hysteresis characteristics, load-bearing capacity, deformability, and strain distribution of STHC composite walls. The test results demonstrated that the bearing capacity and ductility of the STHC composite walls improved with the embedding of steel tubes at the boundary elements. An analytical method was then established to predict the flexural bearing capacity of the STHC composite walls, and the calculated results agreed well with the experimental values, with errors of less than 10%. Finally, a finite element modeling (FEM) was developed via the OpenSees program to analyze the mechanical performance of the STHC composite wall. The FEM was validated through test results; additionally, the influences of the axial load ratio, steel tube strength, and shear-span ratio on the mechanical properties of STHC composite walls were comprehensively investigated.

Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus

( Zhou Liang ),( Jia Min Zhang ),( Xiao Chun Wang ),( Hong Jiang ),( Fu Ming Yi ),( Yuan Yang Hu ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.5

Robust Channel Estimation for OFDM based WLANs

Liang Zhou,Miyoshi Saito 대한전자공학회 2004 ITC-CSCC :International Technical Conference on Ci Vol.2004 No.7

IRE1α protects against osteoarthritis by regulating progranulin-dependent XBP1 splicing and collagen homeostasis

Liang Li,Zhang Fengmei,Feng Naibo,Kuang Biao,Fan Mengtian,Chen Cheng,Pan Yiming,Zhou Pengfei,Geng Nana,Li Xingyue,Xian Menglin,Deng Lin,Li Xiaoli,Kuang Liang,Luo Fengtao,Tan Qiaoyan,Xie Yangli,Guo Fen 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Osteoarthritis (OA) is a full-joint, multifactorial, degenerative and inflammatory disease that seriously affects the quality of life of patients due to its disabling and pain-causing properties. ER stress has been reported to be closely related to the progression of OA. The inositol-requiring enzyme 1α/X-box-binding protein-1 spliced (IRE1α/XBP1s) pathway, which is highly expressed in the chondrocytes of OA patients, promotes the degradation and refolding of abnormal proteins during ER stress and maintains the stability of the ER environment of chondrocytes, but its function and the underlying mechanisms of how it contributes to the progression of OA remain unclear. This study investigates the role of IRE1α/ERN1 in OA. Specific deficiency of ERN1 in chondrocytes spontaneously resulted in OA-like cartilage destruction and accelerated OA progression in a surgically induced arthritis model. Local delivery of AdERN1 relieved degradation of the cartilage matrix and prevented OA development in an ACLT-mediated model. Mechanistically, progranulin (PGRN), an intracellular chaperone, binds to IRE1α, promoting its phosphorylation and splicing of XBP1u to generate XBP1s. XBP1s protects articular cartilage through TNF-α/ERK1/2 signaling and further maintains collagen homeostasis by regulating type II collagen expression. The chondroprotective effect of IRE1α/ERN1 is dependent on PGRN and XBP1s splicing. ERN1 deficiency accelerated cartilage degeneration in OA by reducing PGRN expression and XBP1s splicing, subsequently decreasing collagen II expression and triggering collagen structural abnormalities and an imbalance in collagen homeostasis. This study provides new insights into OA pathogenesis and the UPR and suggests that IRE1α/ERN1 may serve as a potential target for the treatment of joint degenerative diseases, including OA.

HOCl Oxidation-modified CT26 Cell Vaccine Inhibits Colon Tumor Growth in a Mouse Model

Zhou, Rui,Huang, Wen-Jun,Ma, Cong,Zhou, Yan,Yao, Yu-Qin,Wang, Yu-Xi,Gou, Lan-Tu,Yi, Chen,Yang, Jin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.

Expression and Characterization of RNA-dependent RNA Polymerase of Dendrolimus punctatus Tetravirus

Zhou, Liang,Zhang, Jiamin,Wang, Xiaochun,Jiang, Hong,Yi, Fuming,Hu, Yuanyang 생화학분자생물학회 2006 Journal of biochemistry and molecular biology Vol.39 No.5

Dendrolimus punctatus tetravirus (DpTV) has been identified as a new member of the genus Omegatetravirus of the family Tetraviridae that may be related serologically to Nudaurelia capensis virus ($N{\omega}V$). To establish the function of DpTV RNA genome and to better understand the mechanism of viral replication, the putative RNA-dependent RNA polymerase (RdRp) domain has been cloned and expressed in Escherichia coli. The recombinant protein was purified on a Ni-chelating HisTrap affinity column and demonstrated to initiate viral RNA synthesis in a primer-independent manner but not by terminal nucleotidyle transferase activity in the presence of $Mg^{2+}$ and RNA template. Mutation of the GDD to GAA interferes with the residues at the polymerase active site and metal ions, and thus renders the polymerase inactive.

Investigation on fixture design and precision stability of new-type double collect chuck for machining of long ladder shaft gear

Zhipeng Liang,Chunju Zhao,Huawei Zhou,Yihong Zhou 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.1

To solve the problems of poor positioning accuracy and non-automatic processing seen in the machining of long ladder shaft gears, a fixture structure scheme with a new type of double collect chuck (DCC) is proposed. The design rationality, clamping stability, and repeat clamping positioning accuracy of the proposed DCC were analyzed and investigated based on theory calculation, simulation analysis, and engineering application tests. First, the clamping performance of DCC was investigated through theory calculation and simulation analysis. The test results showed that the structure of DCC maintained effective clamping and the repeat clamping positioning accuracy was steadily controlled within 0.008 mm. Meanwhile, efficiently automated machining of long ladder shaft gear was realized based on extended design and engineering application of automatic up and down mechanism when the structure of DCC was applied. Furthermore, the machining accuracy of the gear was steadily controlled within DIN8 (DIN5480-2 standard). Additionally, based on extended design and engineering tests, the automatic up and down machining improves production efficiency and machining accuracy. Thus, the novel fixture structure scheme provides a new approach for automatic up and down machining of long ladder shaft gear.

Modified model for estimation of agglomerate sizes of binary mixed nanoparticles in a vibro-fluidized bed

Xizhen Liang,Jian Wang,Tao Zhou,Hao Duan,Yueming Zhou 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.8

A modified model is established according to the analysis of energy balance acting on an agglomerate of binary mixed nanoparticles in a vibrated fluidized bed (VFB). The sizes of agglomerates of binary mixed nanoparticles are calculated with this model. The average agglomerate size estimated by the model of energy balance decreases with increasing superficial gas velocity. The vibration frequency had a comparatively significant impact on agglomerate sizes that seemed to change regularly and decreased with higher frequency. Both of the experimental and theoretical results showed that vibration led to a smaller agglomerate size, and the average agglomerate sizes calculated by this model provided the closest fit to those determined experimentally.

Method and application of spatial positioning for valid temperature-measuring optical fibers in concrete dams

Huawei Zhou,Chunju Zhao,Zhipeng Liang,Yihong Zhou,Fang Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

Spatial positioning of Valid Temperature-measuring Optical Fiber (VTOF) is of great significancefor accurately grasping the real temperature distribution of a concrete dam. However, there is barely an effective way to accurately locate the measuring points of optical fiber in concrete dams. A comprehensive data symmetry analysis technique is presented for determining the optimal spatial positioning of valid temperature-measuring optical fibers (VTOFs) in concrete dams. This approach includes a mathematical model to confirm the center of symmetry in monitoring data, which is required to reconstruct the real spatial temperature distribution. This process can identify VTOF locations and verify the reliability of temperature predictions. Experimental validation was conducted using findings from research on a new mathematical model, site measurements from a concrete dam, and corresponding data from distributed optical fibers. These results were successfully applied to the construction of the Baihetan dam; the concrete temperature from 405 pouring blocks in 5 typical dam monoliths was accurately monitored, and the temperature-measuring points of VTOFs were more than 24711. Meanwhile, the optical fiber temperature measurement and temperature field analysis system of Baihetan dam located in southwest China was developed based on accurately acquired temperature measurement data, which provides data basis and engineering application platform support for temperature evolution and temperature field analysis and precise temperature control management of pouring blocks.

Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing

Chaoyue Liang,Zhuolin Wu,Xiaohong Gan,Yuanbin Liu,You You,Chenxian Liu,Chengzhi Zhou,Ying Liang,Haiyun Mo,Allen M. Chen,Jiexia Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1

Purpose: This study aimed to identify potential epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer that went undetected by amplification refractory mutation system-Scorpion real-time PCR (ARMS-PCR). Materials and Methods: A total of 200 specimens were obtained from the First Affiliated Hospital of Guangzhou Medical University from August 2014 to August 2015. In total, 100 ARMS-negative and 100 ARMS-positive specimens were evaluated for EGFR gene mutations by Sanger sequencing. The methodology and sensitivity of each method and the outcomes of EGFR-tyrosine kinase inhibitor (TKI) therapy were analyzed. Results: Among the 100 ARMS-PCR-positive samples, 90 were positive by Sanger sequencing, while 10 cases were considered negative, because the mutation abundance was less than 10%. Among the 100 negative cases, three were positive for a rare EGFR mutation by Sanger sequencing. In the curative effect analysis of EGFR-TKIs, the progression-free survival (PFS) analysis based on ARMS and Sanger sequencing results showed no difference. However, the PFS of patients with a high abundance of EGFR mutationwas 12.4 months [95% confidence interval (CI), 11.6−12.4 months], which was significantly higher than that of patients with a low abundance of mutations detected by Sanger sequencing (95% CI, 10.7−11.3 months) (p<0.001). Conclusion: The ARMS method demonstrated higher sensitivity than Sanger sequencing, but was prone to missing mutations due to primer design. Sanger sequencing was able to detect rare EGFR mutations and deemed applicable for confirming EGFR status. A clinical trial evaluating the efficacy of EGFR-TKIs in patients with rare EGFR mutations is needed.