구인 peroxidase의 부분 정제 및 효소학적 특성 규명

이미영,김윤경,이기완 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Peroxidase has been partially purified from earthworm, Lumbricus rubellus, using ammonium sulfate fractionation, Sephacryl S-200 gel filtration, CM-cellulose cation exchange chromatography and native-PAGE elution. Some of its enzymatic characteristics were examined. Earthworm peroxidase showed the characteristics of heat-sensitivity, and the enzyme activity was stimulated in the presence of various cations. The most available booster was CoCl₂, followed by ZnCl₂and KCl among cations used. The optimum pH for guaiacol oxidation of earthworm peroxidase was determined to be 6.0, and the Km values against guaiacol and H₂O₂were 1.25mM and 3.4 mM, respectively. When various compounds were tested as the possible substrates of the enzyme, o-dianisidine was used as the substrate. However, earthworm peroxidase could not oxidize esculetin and ferulic acid as substrates, suggesting the different characteristics of the enzyme from plant peroxidases. The opimum pH for veratryl alcohol and H₂O₂oxidation was determined to be 2.5 when lignin peroxidation activity was examined. The Km values for veratryl alcohol and H₂O₂were 0.02 mM and 0.13 mM, respectively.

GPS와 IMU에 의한 구조물 변형 검색

이재원,백기석,배경호 東亞大學校 大學院 2004 大學院論文集 Vol.29 No.-

Such social structures as bridges, buildings, dams and towers have been transformed by their own load or fundamental ground. They have been behaved by other external causes. These regular or irregular behaviors threaten to do their users safety. Therefore, to monitor the load of the structures or reaction shown by them could help to verify their behaviors. RTK GPS allows the use of a static base station and remote rover unit to allow for data collection within several seconds and in real time. It is useful for monitoring the behaviors of massive structures like bridges. In this Study, Among GPS methods, we used RTK GPS to analyze the precision of monitoring and then on the basis of it, we developed a monitoring system using RTK GPS when measured the behavior of main tower of a suspension bridge by using RTK GPS. Comparing a deviation between observation values, X axis was 1mm, Y axis was 1mm and Z axis 2.2mm. It turned out that it was possible to monitor and measure structures by RTK GPS.

개에서 발생한 다중심성 T 세포 림프종 증례

이기호,박형진,송근호,서경원 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 5-year-old Jindo dog (13.7 kg, intact female) with history of cervical mass was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Complete blood cell count showed mild anemia thrombocytopenia. And serum biochemistry showed increased ALP. On radiologic evaluation, multicentric lymph nodes' enlargement. Based on the findings, the dog was diagnosed as multicentric T-cell lymphoma. The dog was treated with LCOAP-short protocol, but survived 7 months since first finding.

뇌실내출혈로 인한 급성 수두증에서 관찰된 해리증후군

김광기,이경민,이정주,오종배,안태범,정상욱,남현우,박성호 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

정밀여과 공정에서의 역세척에 관한 연구

이기완,최충현,이남경 동신대학교 환경연구소 1999 환경연구 Vol.4 No.1

정수처리 공정의 원수 및 응집/침전 상징수와 하수처리 공정의 원수를 대상으로 MF를 적용하여, 각 시료수의 역세척 특성을 파악하였다. MF의 여과지속기간은 시료수의 투과속도가 1/2로 감소되는 시점과 여과압력은 1 kg/cm²의 조건에서 실험을 수행하였다. MF의 투과속도는 정수 및 하수처리 공정의 원수인 경우 1시간이내, 정수처리 공정의 응집/침전 상징수인 경우 2시간 이내로 나타났다. 정수 및 하수처리 공정의 원수인 경우 MF의 역세 회복율 및 역세 소요시간은 각각98%와 5~6분 및 7~8분으로 나타났다. The backwashing characteristics of three kinds of sample water was studied with the application of MF(Micro Filtration) process. The three kinds are raw water and clarified water at water treatment process and raw water at sewage treatment process. The tests were performed with the filtering duration of MF to the half permeability arriving time and the filtering pressure 1 kg/㎠. The MF permeability of the raw water at the both process was less than 1 hour and that of the clarified water at water treatment process was less than 2 hour. The recovery rate and the consume time of backwashing for the raw water were 98%, 5-6 minutes and 99%, 7-8 minutes at the water and sewage treatment process, respectively.

횡성댐 水沒地域內의 植生에 關한 硏究 : 移植對象區域을 中心으로 Centered with Subdivided Area to Select Plants Capable of Transfer

이우철,이기의,서옥하,조현길,백원기,김경진 江原大學校 森林科學硏究所 1997 Journal of Forest Science Vol.13 No.-

본 조사 연구는 江原道 橫成郡 甲川面과 橫成邑 일부를 포함하는 지역에 횡성댐이 건설됨에 따라 수몰되어질 自然生態系의 復元과 保存計劃의 일부로 수행되어졌다. 移植對象區域인 7개소를 중심으로 綠地自然度, 植生現存量 및 생산량 種組成과 植生에 관해 조사하였으며 그 결과는 다음과 같다. 1. 綠地自然度를 등급별로 사정한 결과 등급 0(3.01%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), 및 8(13.8%)의 8개 등급으로 구분되었다. 2. 전체조사지역의 現存量과 生産量은 각각 88,436.3 ton/year 과 12,960.3 ton/year 이었다. 3. 조사지역의 陸上植物의 種組成은 86科, 221屬, 306種, 1變種, 3品種의 총 310種으로 조사되었다. 4. 선정된 7개의 植生調査地點에서는 소나무군락이 憂占하며, 그밖에 신갈나무군락, 떡갈나무군락, 상수리나무군락으로 나타났다. This study was carried out in a part of conservative plans recovering natural ecosystem which will be submerged, as Hoengseong dam is being constructed around Hoengseong-Eup and Gabcheon-Myun. The submerged area was divided into 7 small areas to figure out what kinds of species of plants are and will be according to detailed geographic characteristics. In the center of the subdivided areas. Degree of Green Naturality, standing biomass, net 36production per year, and composition of plant species were investigated. The results are as following; 1. The surveyed area was classified into 8 degree by Degree of Green Naturality, and the appeared degree and the rate of occupation were 0(3.01%), 1(5.3%), 2(28.2%), 3(0.1%), 5(0.2%), 7(4.6%), 7(44.7%), 및 8(13.8%). 2. Standing biomass was 88,436.3 ton/year and net production per year was 12,960.3 ton/year. 3. The list of vascular plants identified in this study consisted of 86 families, 221genera, 1 variety, 3 formae and 306 species, totally summiting upto 310 species. 4. The 7 surveyed areas were largely occupied by Pinus community. In addition, there were Quercus mongolica, Quercus dentata, and Quercus acutissima communities.

통풍 환자에서의 지질대사

이은우,강기서,강수용,이한준,김경환,임정일,박철경 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.2

Objective: To evaluate hyperlipidemia, a msjor tisk factor of coronary artery disease, frequently asscoiated with gout patients, we compared the mean lipid level of gout patient with that of non-gout male and accessed statistical significance for treatment of gout patient. Method: This study ws performed in the 266 male gout outpatients of the CAU medical center, who had serum lipid level test from Apr. 1996 to Aug. 2000. Serum uric acid, total cholesterol, triglyceride, HDL -cholesterol, and LDL-cholesterol level were measured after 12 hours of fasting. The patients with high lipid level were further evaluated into each type of the hyperlipidemia through electrophoresis study, and the test results were compared with that of the non-gout male adults(40∼60yr), and the statistical significance was examined. Results : The gout patients showed high levels of total cholesterol, triglyceride, and LDL-cholesterol. Especially they showed high level of triglyceride and LDL-cholesterol which suggested as the risk factors of atherosclerosis and coronary heart disease, but shoewed low level of HDL -cholesterol. But there were no definite relations between lipid level and other factors auch as age of patient, initial uric acid ;eve; and duration od gout. Conclusion: To evaluate the risk factors of atherosclerotic change of cadriac vascular system in gout patient, screening tests of serum lipid level of the patient seem mandatory.

A case of atypical Spitz nevi

( Kyoung Geun Lee ),( Won Choi ),( Joon Soo Lim ),( Seung Hyun Cheong ),( Ki Bum Myung ),( Hyung Jin Hahn ) 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.1

Spitzoid melanocytic lesions may be broadly categorized into Spitz nevi, atypical Spitz tumor (AST), and spitzoid melanomas. Atypical Spitz nevi, also known as AST is rare spitzoid melanocytic proliferations with an uncertain malignant potential and described as conventional Spitz nevi with 1 or more atypical features with indeterminate biological potential. It has clinical features of larger size (>6 mm), irregular borders, irregular topography, or ulceration. Threre are no definite histologic diagnostic criterias among spitzoid melanocytic proliferations, but asymmetry, ulceration, pagetoid melanocytosis, lack of maturation, deep mitoses, and atypia are distinct findings of atypical Spitz nevi. A 2-year-old man presented with several months of pigmented nodule on right knee. Skin biopsy from right knee showed spitzoid melanocytic proliferation from the epidermis to the reticular dermis. Melanocytes arranged in nests were epithelioid with abundant eosinophilic cytoplasm. Each cells showed uniform pleomorphism and mitosis, but there were no atypical mitosis. Few kamino bodies in epidermis and outlier cells in reticular dermis were also detected. Immunohistochemical study revealed positive of HMB-45, S-100, P16 and Ki-67 over 30 percent. We report a case of atypical spitz nevi and distinguishing features of spitzoid melanocytic proliferations through literature review.

특수형 방사성 동위원소 운반캡슐의 안정성 평가

이주찬,서기석,구정회,방경식,한현수,박성원 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.1

특수형 방사성물질 운반캡슐은 국내외의 수송관련 법규에 규정된 기술기준을 만족하도록 설계, 제작되어야 한다. 본 연구의 목적은 하나로에서 생산된 192Ir 특수형 동위원소 운반캡슐의 건전성을 평가하는데 있다. 법규에서 규정된 낙하시험, 타격시험, 굽힘시헝 및 가열시험조건에 대한 안전성 시험을 수행하였으며, 각각의 시험 전후에 누설시험을 수행하였다. 또한, 안전성시험과 더불어 컴퓨터코드를 이용한 전산해석을 수행하여 안전성시험 전에 시험결과에 대한 예측자료로 활용되었다. 낙하시험 및 가열시험 결과 캡슐 표면에서 약간의 흠집과 변형이 발생하였으나, 각각의 시험에서 평가기준이 되는 캡슐의 손상이나 용융 등은 발생하지 않았다. 또한 각 시험 후 수행한 누설시험 결과 누설이 발생하지 않았다. 따라서 특수형 방사성물질 운반캡슐은 법규에서 규정하는 기술기준을 만족하도록 설계, 제작되었음이 입증되었다. All of sealing capsules to transport a special form radioactive material should be designed and fabricated in accordance with the design criteria prescribed in IAEA standards and domestic regulations. The objective of this study is to demonstrate the safety of a shipping capsule for 192Ir special form radioisotope which produceed in the HANARO. The safety tests were carried out for the impact, percussion, bending and heat test conditions. And leakage tests were carried out before and after the each test. Also, the safety analyses were performed using computer codes in order to verify the test results. The capsule showed slight scratches and deformation, and maintained its structural and thermal integrities in all tests without any severe damage or melting. It also met the allowable limits of leakage rate after earth test. Therefore, it has been verified that the capsule was designed and fabricated to meet all requirements for the special form.

만성 요통증 환자에 대한 록소닌 (Loxoprofen sodium)의 임상적 효과에 대한 연구

이준규,김경태,오기영 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.1

We conducted a clinical effectiveness and safety of Loxoprofen sodium, a nonsteroidal antiinflammatory drug(NSAID) for the treatment of chronic low back pain. Loxoprofen sodiun 180 mg/day(60 mg three time daily) was administered to 30 patients with chronic low back pain associated with a range of arthritic disorders, for a period up to 4 weeks, and clinical efficacy was evaluated by objective and subjective methods. 4 weeks therapy, loxoprofen showed subjective improvements of 100%, 64.7% and 63.6% in patients with mild to moderate, moderate to severe, and severe lumbar pain etc, respectively. Objective and subjective improvements were significant after only 1 week therapy in patients with mild to moderate pain and after 4 weeks, over all intensities of pain were improved. The incidence of adverse effects over this study population was extremely low, affecting only 3.3% of patients, and were mild in nature and edematous change. Loxoprofen is thus a well-tolerated NSAID in the treatment of chronic lumbar pain.