Development of Visualization Tool for Learning Introductory Algorithms

Kyeong-Sug Oh,Nam-Hoon Ryu,Hye-Mi Lee,Sang-Jin Lee,Eung-Kon Kim,Kyoung-Wook Park 한국정보통신학회 2010 2016 INTERNATIONAL CONFERENCE Vol.3 No.1

First Report on Tomato bushy stunt virus Infecting Tomato in Korea

Mi-Kyeong Kim,Hae-Ryun Kwak,Seon-Gi Jeong,Sug-Ju Ko,Su-Heon Lee,Jin-Woo Park,김국형,최홍수,차병진 한국식물병리학회 2007 Plant Pathology Journal Vol.23 No.3

A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

국내 병원의 근거기반간호 수행 현황과 한국근거기반간호학회의 역할

박경희(Park, Kyung Hee),정재심(Jeong, Jae Sim),김주현(Kim, Joo-Hyun),권정순(Kwon, Jeong Soon),김경숙(Kim, Kyeong Sug),홍은영(Hong, Eun-Young),이현희(Lee, Hyun Hee),정영선(Jung, Young Sun),류재금(Ryu, Jae Geum),정인숙(Jeong, Ihn So 한국근거기반간호학회 2018 근거와 간호 Vol.6 No.1

Purpose: This qualitative study was done to identify the current status for evidence-based nursing (EBN) and the roles of the Korean Society of EBN (KEBN) for facilitating EBN. Methods: The participants were 7 female nurses who have worked in the acute care hospitals. Data were collected through focused group interviews. Collected data were analyzed with content analysis method. Results: The findings can be categorized into five main themes. 1) Background for introducing of EBN. 2) Facilitators to spread EBN. 3) Barriers to spread EBN. 4) Changes after introducing EBN into clinical practice. 5) Roles of the KEBN. Conclusion: The current status of EBN is that awareness about the importance of EBN is high and knowledge with skills are improving gradually. However, it is necessary to acquire solid knowledge and skills to facilitate their own EBN within the institutions. Therefore, we expect the roles of the KEBN in sharing and standardizing information of EBN with other organizations. The KEBN specifically may consider to operate a EBN program for mentors and it is necessary to seek strategies to plan a platform that can build a standardized system through cooperation with department of nursing to share and spread EBN related research.

Isolation and Characterization of Pepper mottle virus Infecting Tomato in Korea

Mi-Kyeong Kim,곽해련,Jung-Heon Han,Sug-Ju Ko,이수헌,Jin-Woo Park,Miranda Gilda Jonson,김국형,Jeong-Soo Kim,최홍수,차병진 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.2

A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnamdo, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PepMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3'-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoV and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19% were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sapinfected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750 nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoV and only <61% with PVY. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

해산어류에서 분리된 Vibrio harveyi의 혈청학적 특성과 항원성

오윤경 ( Yun Kyeong Oh ),김명석 ( Myoung Sug Kim ),박명애 ( Myoung Ae Park ),김진우 ( Jin Woo Kim ),조지영 ( Ji Young Cho ),정현도 ( Hyun Do Jeong ) 한국어병학회 2009 한국어병학회지 Vol.22 No.1

Overexpression of Cuphea viscosissima CvFatB4 enhances 16:0 fatty acid accumulation in Arabidopsis

Yeon, Jinouk,Park, Jong-Sug,Lee, Sang Ho,Lee, Kyeong-Ryeol,Yi, Hankuil The Korean Society of Plant Biotechnology 2019 식물생명공학회지 Vol.46 No.4

Cuphea viscosissima plants accumulate medium-chain fatty acids (MCFAs), i.e., those containing 8 ~ 14 carbons, in their seeds, in addition to the longer carbon chain fatty acids (≥16 carbons) found in a variety of plant species. Previous studies have reported the existence of three C. viscosissima MCFA-producing acyl-acyl carrier protein (ACP) thioesterases with different substrate specificities. In this study, CvFatB4, a novel cDNA clone encoding an acyl-ACP thioesterase (EC 3.1.2.14), was isolated from developing C. viscosissima seeds. Sequence alignment of the deduced amino acid sequence revealed that four catalytic residues for thioesterase activity are conserved and a putative N-terminal chloroplast transit peptide is present. Overexpression of CvFatB4 cDNA, which was under the control of the cauliflower mosaic virus 35S promoter, in Arabidopsis thaliana led to an increase in 16:0 fatty acid (palmitate) levels in the seed oil at the expense of 18:1 and other non-MCFAs.

Isolation and Characterization of Pepper mottle virus Infecting Tomato in Korea

Kim, Mi-Kyeong,Kwak, Hae-Ryun,Han, Jung-Heon,Ko, Sug-Ju,Lee, Su-Heon,Park, Jin-Woo,Jonson, Miranda Gilda,Kim, Kook-Hyung,Kim, Jeong-Soo,Choi, Hong-Soo,Cha, Byeong-Jin The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.2

A peculiar virus-like disease of tomato showing yellow mosaic and necrotic spots on leaves and necrosis on veins, petioles and stems was observed at the Tomato Experimental Station (TES), Buyeo, Chungcheongnamdo, Korea. The disease incidence at TES fields ranged from 21 to 35% infecting different tomato cultivars. For this reason, to identify the virus infecting tomato and to characterize the virus based on biology, serology, cytology and at molecular level. Here, leaf samples were randomly collected from different infected tomato cultivars at TES fields and greenhouses and tested by ELISA using Pepper mottle virus (PePMoV) and Tomato mosaic virus (ToMV) antisera. Infected saps were mechanically inoculated in different host plants to test for pathogenicity, symptomatology and host ranges. Infected tissues and ultrathin sections were examined by electron microscopy. Finally, putative coat protein and 3'-untranslated region (CP/3'-UTR) fragment was amplified and cloned for sequence determination and analyzed its genetic relationship to existing PepMoV and PVY sequences at the Genbank. Results showed 69% of the samples were positive with PepMoV, 13% with ToMV and 19 % were doubly infected with PepMoV and ToMV. Symptoms greatly varied from different host plants inoculated with tomato leaf sap infected with PepMoV alone and discussed in detailed in this paper. Electron microscopy from infected tissues showed filamentous particles of 720-750nm in length, a typical morphology and size of PepMoV. In addition, cylindrical inclusion bodies, pinwheels, scrolls and laminates with masses of fibrillar inclusions were also found in ultrathin sections. Alignment of the sequences of the CP/3'-UTR revealed >96% sequence identity with PepMoV and only <61% with PVY. Taken together, all these evidences presented clearly indicated that the causal agent infecting tomato at TES was PepMoV and we designated this PepMoV infecting tomato as Tom-sd2 strain in this study.

First Report on Tomato bushy stunt virus Infecting Tomato in Korea

Kim, Mi-Kyeong,Kwak, Hae-Ryun,Jeong, Seon-Gi,Ko, Sug-Ju,Lee, Su-Heon,Park, Jin-Woo,Kim, Kook-Hyung,Choi, Hong-Soo,Cha, Byeong-Jin The Korean Society of Plant Pathology 2007 Plant Pathology Journal Vol.23 No.3

A new virus-like disease of tomato showing chlorotic spots, malformation and necrosis on leaves, and chlorotic blotching, rings, and necrosis on fruits was observed around Sacheon, Gyeongsangnam-do, Korea in 2004. Host range analysis could not differentiate 4 field isolates collected from tomatoes showing different symptoms but identified them as Tomato bushy stunt virus (TBSV). TBSV-tsf2 isolate induced symptoms in indicator plants similar to those caused by the TBSV-C, -S and -Nf. As the isolate could not systemically infect Chenopodium quinoa, the isolate might belong to the previously described TBSV-S isolate. TBSV-tsf2 isolate caused similar cytological alterations that were similar to that generally caused by previously reported TBSV isolates. TBSV-tsf2 isolate, however, could be considered to belong to new strain of TBSV because masses of small electron-dense patches that were not observed from the previously described TBSV. The complete nucleotide sequences of the genomic RNA of 4739 nt excluding non-translated sequences at both termini have been determined and compared to sequences of other TBSV strains. The complete nucleotide sequence identity among TBSV isolates was 98.9% to 99.7%, and to the other tombusviruses ranged from 80.8% to 94.9%. Comparison of the amino acid sequences all five ORFs with those of other TBSV strains shows a similar genomic organization, and high percentage of amino acid sequence homology with TBSV-Nf than TBSV-S isolate. Since the TBSV symptoms were only observed in Sacheon fields where imported seeds from Japan were planted, the TBSV incidence probably caused by the planting contaminated tomato seeds and thus require more through quarantine procedure to prevent settlement of TBSV in Korea. Altogether, these results support that the Korean isolate of TBSV infecting tomato might be new strain.

염화알미늄 증기의 부분가수분해를 통한 알파 알루미나 나노입자 제조

박회경 ( Hoey Kyung Park ),유연석 ( Youn Sug Yoo ),박균영 ( Kyun Young Park ),정경열 ( Kyeong Youl Jung ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.5

Spherical alumina precursors represented by (AlO)x(Cl)y(OH)z, 30~200 nm in particle diameter, were prepared by partial hydrolysis of AlCl3 vapor in a 500 ml reactor. Investigated on the particle morphology and size were the effects of the reaction time, the stirring speed and the reaction temperature. The particle morphology and size was insensitive to the reaction time in the range 20 to 300 s. The variation of the stirring speed from 0 to 300 and 800 rpm showed that the particle size was the largest at 0 rpm. As the temperature was varied from 180 to 190, 200, 140 °C, the particle size showed a maximum at 190 ˚C. By calcination of the as-produced particles at 1,200 °C for 6h with a heating rate of 10 °C/min, α-alumina particles 45 nm in surface area equivalent diameter were obtained. The particle shape after calcination turned wormlike due to sintering between neighboring particles. A rapid calcination at 1400 °C for 0.5 h with a higher heating rate of 50 °C/min reduced the sintering considerably. An addition of SiCl4 or TMCTS(2,4,6,8-tetramethylcyclosiloxane) to the AlCl3 reduced the sintering effectively in the calcination step; however, peaks of γ or mullite phase appeared. An addition of AlF3 to the particles obtained from the hydrolysis resulted in a hexagonal disc shaped alumina particles.

Characteristics of Potato virus Y Isolated from Paprika in Korea

Choi, Hong-Soo,Ko, Sug-Ju,Kim, Mi-Kyeong,Park, Jin-Woo,Lee, Su-Heon,Kim, Kook-Hyung,Were, Hassan Karakacha,Chois, Jang-Kyung,Takanami, Yoichi The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4

A virus isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Potato virus Y (PVY) based on biological, serological, cytopathological, and molecular properties. In host range studies, the paprika isolate produced the mosaic symptom on some tobacco, tomato and pepper (Capsicum annuum). A new paprika isolate also infected potato cultivars which is different biological characteristic compared to the other popular potyvirus infecting paprika, Pepper mottle virus (PepMoV). Previously reported PVY strains, $PVY^o$ and $PVY^N$ did not infect pepper and typical PepMoV isolates did not infect potato. Distinctive inclusion patterns of the scroll, pinwheel, long laminated inclusions, and helper components in the cytoplasm of infected cells were also different to those observed by the typical PVY isolate infections. However, the paprika isolate reacted to the monoclonal antibody of $PVY^N$ strain with high absorbance readings. RT-PCR amplification, cloning, and sequencing of the 3' untranslated region and a part of coat protein gene also added additional evidence of the paprika isolate as the $PVY^N$-related isolate. Multiple alignments as well as cluster dendrograms of PVY-paprika isolate revealed close phylogenetic relationship to the $PVY^N$ subgroup. Altogether, these results suggest that a new PVY isolate infecting paprika contained distinct characteristics compared to the other previously described PVY strains with closer relationship to the $PVY^N$ strain.