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Katayama, Kazunori,Fuchu, Hidetaka,Sugiyama, Masaaki,Kawahara, Satoshi,Yamauchi, Kiyoshi,Kawamura, Yukio,Muguruma, Michio Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.9
In order to clarify one of the biological functions of pork, we investigated whether a peptic hydrolysate of denatured porcine crude myosin showed inhibitory activity against angiotensin I-converting enzyme (ACE), which contributed to hypertension. Our results indicated that this hydrolysate showed relatively strong activity, and we therefore attempted to separate the involved peptides, which were considered to be active substances. To isolate these active peptides, the hydrolysate was separated using a solidphase separation, gel filtration high-performance liquid chromatography (HPLC), and two kinds of reverse phase HPLC. In each stage of separation, many fractions were detected, almost all of which showed ACE inhibitory activity. Thus, we suggested that the activity of the hydrolysate as a whole was a result of the activities of the many individual peptides. Six peaks were distinguished, with yields from 34 to 596 ppm of original crude myosin. In addition to the six peaks, many other active fractions were found throughout the separation steps, strongly suggesting that whole porcine crude myosin itself had ACE inhibitory activity. Moreover, pork as food was considered to function as an ACE inhibitory material in vivo, because pork proteins consist primarily of crude myosin, which included almost all the myofibrillar structural proteins.
Collimator Focus Adjustment for Free Space Optics System Using Single-mode Optical Fibers
Koichi Yoshida,Kuniaki Tanaka,Kazunori Katayama,Takeshi Tsujimura,Yuji Azuma 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper presents a method for adjusting a free space optics (FSO) system that links two single-mode optical fibers (SMFs) using collimators. It operates as if the two SMFs were seamlessly connected without the need for any photoelectric devices. Collimator focusing as well as axis alignment is critical for low loss transmission and is performed using an opto-mechatronic system in the proposed method.
Masayoshi Seike,Tetsuya Fukushima,Kazunori Sato,Hiroshi Katayama-Yoshida 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.12
We present a materials design of MgO-based d0 ferromagnets with a high blocking temperature(TB) based on multiscale simulations. The chemical pair interactions between the N atoms inMg(O,N) and the Mg vacancies (VMg) in (Mg,VMg)O were calculated using a generalized gradientapproximation, and Monte Carlo simulations of the crystal growth were performed to predictthe configurations of the dopant distribution. Our simulations showed that self-organized nanostructurescould be formed both in Mg(O,N) and (Mg,VMg)O, which suggests that a high TB canbe obtained in these d0 ferromagnets due to the superparamagnetic blocking phenomenon. Furthermore,depending on the crystal growth conditions, it was shown that various self-organizednanostructures, such as three-dimensional nanoclusters and one-dimensional nanowires, could appear.
High-Resolution Optical Measurement of Fiber Optics Transmission Line Length
Takeshi Tsujimura,Koichi Yoshida,Kuniaki Tanaka,Kazunori Katayama,Yuji Azuma,Masahiko Mikawa 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper studies a frame-loss-free shunt system for broadband optical fiber transmission lines. This system switches transmitting optical pulses between duplicated optical fibers to avoid any interruption to communication. A high-resolution measurement technique is necessary to evaluate the optical fiber length. We applied an interferometry technique to adjust 15 km duplicated optical lines at a resolution of 10 mm. An optical line switching system and procedure are designed for use in replacing in-service optical lines. A free space optics (FSO) system adjusts the length of an in-service optical line by up to 100 m. A gigabit ether-passive optical network (GE-PON) line is successfully replaced with an extended line while maintaining transmission.
Effect of Heating on Polymerization of Pig Skin Collagen Using Microbial Transglutaminase
Erwanto, Yuny,Muguruma, Michio,Kawahara, Satoshi,Tsutsumi, Takahiko,Katayama, Kazunori,Yamauchi, Kiyoshi,Morishita, Toshiro,Morishita, Toshiro,Watanabe, Shohei Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.8
Polymerization of heated or unheated pig skin collagen using microbial transglutaminase (MTGase) was investigated. Pig skin collagen samples were heated or left unheated, then enzymatically polymerized with MTGase. SDS-PAGE was conducted to confirm the intermolecular polymer and the results showed similar bands between samples without MTGase and unheated samples with MTGase. The polymerized product of pig skin collagen was not formed in unheated samples, even when MTGase was added during incubation. Different results were obtained from samples heated at $80^{\circ}C$ and $100^{\circ}C$ for 2 min, whereas the SDS-PAGE pattern indicated that a polymer band was generated in both cases. The heat treatment successfully modified the native structure of collagen and also made collagen more reactable in the MTGase polymerization system. Scanning Electron Microscope (SEM) investigation of pig skin collagen showed a biopolymer structure through intermolecular collagen crosslinking, while there were no intermolecular crosslinks in samples not treated with MTGase. There were no significant differences in fibril diameter between treated samples and controls. These results suggest that heat treatment of native pig skin collagen enhanced the polymerization capability of MTGase.