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Establishment of a canine mammary gland tumor cell line and characterization of its miRNA expression
Tomohiro Osaki,Yuji Sunden,Akihiko Sugiyama,Kazuo Azuma,Yusuke Murahata,Takeshi Tsuka,Norihiko Itoh,Tomohiro Imagawa,Yoshiharu Okamoto 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.3
Canine mammary gland tumors (CMGTs), which are the most common neoplasms in sexually intact female dogs, have been suggested as a model for studying human breast cancer because of several similarities, including relative age of onset, risk factors, incidence, histological and molecular features, biological behavior, metastatic pattern, and responses to therapy. In the present study, we established a new cell line, the SNP cell line, from a CMGT. A tumor formed in each NOD.CB17-Prkdcscid/J mouse at the site of subcutaneous SNP cell injection. SNP cells are characterized by proliferation in a tubulopapillary pattern and are vimentin positive. Moreover, we examined miRNA expression in the cultured cells and found that the expression values of miRNA-143 and miRNA-138a showed the greatest increase and decrease, respectively, of all miRNAs observed, indicating that these miRNAs might play a significant role in the malignancy of SNP cells. Overall, the results of this study indicate that SNP cells might serve as a model for future genetic analysis and clinical treatments of human breast tumors.
High-Resolution Optical Measurement of Fiber Optics Transmission Line Length
Takeshi Tsujimura,Koichi Yoshida,Kuniaki Tanaka,Kazunori Katayama,Yuji Azuma,Masahiko Mikawa 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper studies a frame-loss-free shunt system for broadband optical fiber transmission lines. This system switches transmitting optical pulses between duplicated optical fibers to avoid any interruption to communication. A high-resolution measurement technique is necessary to evaluate the optical fiber length. We applied an interferometry technique to adjust 15 km duplicated optical lines at a resolution of 10 mm. An optical line switching system and procedure are designed for use in replacing in-service optical lines. A free space optics (FSO) system adjusts the length of an in-service optical line by up to 100 m. A gigabit ether-passive optical network (GE-PON) line is successfully replaced with an extended line while maintaining transmission.
Unilateral rostral mandibulectomy for gingival vascular hamartoma in two calves
Takeshi Tsuka,Yoshiharu Okamoto,Naoki Yamamoto,Keiji Hayashi,Takehito Morita,Yuji Sunden,Yusuke Murahata,Kazuo Azuma,Tomohiro Osaki,Norihiko Ito,Tomohiro Imagawa 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.4
A 2-month-old female Holstein calf and a 5-month-old female Japanese black calf presented with gingival vascular hamartoma located in the interdental space between the second and third mandibular incisors in the right and left mandibles, respectively. On radiographic or computed tomographic images, osteolytic changes appeared within the mandibular bones adjacent to the masses. The masses were removed along with affected mandibular bone by using unilateral rostral mandibulectomy. After surgery, both cases exhibited a normal appetite and grew normally, with no cosmetic changes or recurrences. Unilateral rostral mandibulectomy can be applied for invasive gingival vascular hamartomas associated with osteolytic changes.
Collimator Focus Adjustment for Free Space Optics System Using Single-mode Optical Fibers
Koichi Yoshida,Kuniaki Tanaka,Kazunori Katayama,Takeshi Tsujimura,Yuji Azuma 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8
This paper presents a method for adjusting a free space optics (FSO) system that links two single-mode optical fibers (SMFs) using collimators. It operates as if the two SMFs were seamlessly connected without the need for any photoelectric devices. Collimator focusing as well as axis alignment is critical for low loss transmission and is performed using an opto-mechatronic system in the proposed method.