Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

[논문]실시간 정밀 3차원 지형측량기법을 이용한 저수량산출시스템 개발

송석진,장용구,김상석,강인준 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

현재 고정밀도 저수량 측량에 사용되는 측량장비에는 수평위치결정방법으로 실시간 DGPS측량장비가 많이 활용되고 있고, 수심측량에는 디지털 음향측심기를 많이 사용하고 있다. 본 연구에서는 현재, 수심측량 결과처리 시 문제가 되고 있는 소프트웨어의 가격부담을 줄이고 수심측량과 내업처리에 의한 저수량산출을 통합처리 할 수 있는 국내 자체개발의 저가형 외 · 내업 일체형 저수량산출시스댐을 개발하였다.

산업장 근로자의 표준체중치

정종학,강복수,김석범,사공준,이정길,한구웅 大韓産業醫學會 1989 대한직업환경의학회지 Vol.1 No.1

산업장 근로자의 건강증진과 노동력보존에 기여할 목적으로 경상북도 구미시에 소재하는 구미공단내의 20-29세 산업장 근로자 10,407명(남자 6,201명. 여자 4,206명)을 대상으로 1985년의 정기 신체 검사 자료로 부터 성별. 연령별 평균신장, 평균체중 및 신장과 체중간의 상관관계와 회귀방정식을 구하고 이들을 이용하여 신장별 표준체중과 이상체중의 범위를 구했으며 신장과 체중으로 BMI를 산출하였다. 20대 근로자의 평균신장은 남자가 168.2±5.61cm였고 여자는 155.9±5.26cm였고 평균체중은 남자가 61.4±6.56kg, 여자는 52.4±6.00kg이었다. 신장과 체중간의 상관계수는 남자의 경우 +0.541이었고 회귀방정식은 Y(체중)=0.637Ⅹ(신장)-44.975였으며 여자는 상관계수가 +0.559였고 회귀방정식은 Y(체중)=0.637 Ⅹ(신장)-46.898 이었다. 신장별 표준체중은 신장이 155cm일때에 남녀 각각 53.0kg, 51.8kg 이었으며 165 cm 일때는 59.3kg, 58.2kg이었고 175cm일때에 65.6kg, 64.6kg이었고, 정상체중의 범위는 신장이 155cm일때에 남자는 47.5-58.5kg, 여자는 46.9-56.8kg, 165cm일때는 남자는 53.8-64.8kg, 여자는 53.2-63.2kg, 175cm일때에 남자는 60.1-72.1kg, 여자는 59.6-69.6kg으로 평가되었으며, 비만은 신장이 155cm 일때에 남자는 64.1kg이상, 여자는 61.8kg이상, 165cm일때는 남자는 70.3kg이상, 여자는 68.2kg이상, 175cm일때에 남자는 76.7kg이상, 여자는 74.5kg 이상으로 평가되었다. 성별. 연령별 BMI는 남자가 21.7±1.95였고 여자는 21.6±2.05였고 Garrow의 BMI 분류방식에 따른 desirable range에 속하는 근로자는 남자가 75.9%였고, 여자는 71.3%였다. To contribute to promotion of health and preservation of labor power of Korean laborers, the body height and body weight were measured for 10,407 workers(6,201 male, 4,206 female) in the age group of 20-29, engaged in manufactures in the Gumi industrial complex, Gumi city, Kyungpook province. The above data were extracted from the 1985 periodic examination chart for calculation of the mean body weight, mean body height, correlation coefficient and regression equation between weight and height, standard body weight, body mass index(BMI) and distribution of laborers within Garrow's classification of BMI by age and sex group. Mean body height of 20-29 age group was 168.2±5.61 cm for male and 155.9±5.26 cm for female. Mean body weight of 20-29 age group was 61.4±6.56 kg for male and 52.4±6.00 kg for female. Correlation coefficient and regression equation of 20-29 age group were +0.541 and Y(Wt)=0.632X(Ht)-44.975 for male and +0.559 and Y(Wt)=0.637X(Ht)-46.898 for female. Standard body weight of 20-29 age group was 53.0 kg at 155 cm, 59.3 kg at 165 cm, 65.6 kg at 175 cm for male and 51.8 kg at 155 cm, 58.2 kg at 165 cm at 64.6 kg at 175 cm for female. Range of normal body weight of 20-29 age group was 47.5-58.5 kg at 155 cm, 53.8-64.8 kg at 165 cm, 60.1-72.1 kg at 175 cm for male and 46.9-56.8 kg at 155cm, 53.2-63.2 kg at 165 cm, 59.6-69.6 kg at 175 cm for female. Range of obesity of 20-29 age group was 64.1 kg and over at 155 cm, 70.3 kg and over at 165 cm, 76.7 kg and over at 175 cm for male and 61.8 kg and over at 155 cm, 68.2 kg and over at 165 cm, 74.5 kg and over at 175 cm for female. Body mass index(kg/m²) of 20-29 age group was 21.7±1.95 for male and 21.6±2.05 for female, 75.9% of male laborers and 71.3% of famale counterparts fall in the desirable range of BMI by Garrow's classification.

고빌리루빈혈증을 동반한 자가면역성 간염 1례

서영범,김성욱,장재식,강혁주,이중현,윤병구,김욱년,이광헌,이구,유석동,양창헌,이정호,이영현,이창우,서정일 동국대학교 의학연구소 2000 東國醫學 Vol.7 No.-

자가면역성 간염은 대개 만성 경과를 가지며, 혈중 자가면역항체와 혈청 글로불린치의 상승, 그리고 조직학적으로 괴사 염증성 변화를 특징으로 하는 질환으로 아직 정확한 병인이 밝혀져 있지 않은 상태이다. 발병연령은 대개 젊은 영자(15-25세)에서 호발한다. 이 질환은 급성 간염의 임상경과를 보일 수 있으나, 심한 급성 간염이나 전격성 간염으로도 나타날 수 있는데, 이 경우 아주 나쁜 예후를 보인다고 한다. 치료는 자가면역성 간염 임상 경과의 다양성이나 병인, 병리기전의 불확실성에도 불구하고 대개 steroid 치료에 반응하는 것으로 알려져 있으며 대개 80%의 관해율을 나타내며 궁극적으로 간경변으로의 진행을 막을 수 있는 것으로 보인다. 저자들은 58세 남자에서 발생한 급성의 경과를 가지고 심한 황달을 동반한 자가면역성 간염을 경험하였기에 문헌고찰과 함께 보고하는 바이다. Autoimmune hepatitis (AIH) is a chronic necroinflammatory liver disorder of unknown cause associated with circulating autoantibodies and a high serum globulin level. The age of onset of AIH show a peak between the age of 15 and 25 years. AIH can develop and be manifested as acute hepatitis, but severe form of acute hepatitis or fulminant hepatic failure has a poor prognosis. Although AIH is likely to progress from chronic active hepatitis to cirrhosis, steroid therapy can control the disease activity, prolong survival, improve the quality of life , and defer liver transplantation. In the present report we describe a 58-year-old man who admitted because of progressive jaundice and fatigue. He was diagnosed with AIH from laboratory test result showing positivity for antinuclear antibodies, anti-smooth muscle antibodies, and negativity for hepatitis viral markers and from liver biopsy. Steroid therapy, oral administration of prednisolone, was effective in improving the liver function test. Following liver biopsy 6 months after onset shows markedly improved necroinflammatory activity.

Surgical Management and Outcome of Tethered Cord Syndrome in School-Aged Children, Adolescents, and Young Adults

Kang, Joon-Ki,Yoon, Kang-Jun,Ha, Sang-Su,Lee, II-Woo,Jeun, Sin-Soo,Kang, Seok-Gu The Korean Neurosurgical Society 2009 Journal of Korean neurosurgical society Vol.46 No.5

Objective : The adolescent presentation of tethered cord syndrome (TCS) is well-recognized, but continues to pose significant diagnostic and management controversies. The authors conducted a retrospective study of clinical outcomes after surgical intervention in 24 school-aged children, adolescents, and young adults with TCS. Methods : All 83 patients with a lipomyelomeningocele (LMMC) underwent untethering surgery for caudal cord tethering between 1987 and 2007. The clinical charts and follow-up data were reviewed. Of these patients, 24 school-aged children, adolescents, and young adults with TCS were studied with respect to the clinical, radiologic, pathologic features, and surgical outcomes. Results : Untethering procedures were performed in 24 patients (age range, 7-25 years) for TCS of various origins (lipoma, lipomyelomeningocele, and tight filum terminale). Specific circumstances involving additional tugging of the already tight conus, and direct trauma to the back precipitated the onset of symptom in 50% of the patients. Diffuse and non-dermatomal leg pain, often referred to the anorectal region, was the most common presenting symptom. Progressive sensorimotor deficits in the lower extremities, as well as bladder and bowel dysfunction, were also common findings, but progressive foot and spinal deformities were noted less frequently. The most common tethered lesions were intradural lipomas, thickened filum and fibrous band adhesions into the placode sac. The surgical outcome was gratifying in relation to pain and motor weakness, but disappointing with respect to resolution of bowel and bladder dysfunction. Of the 24 patients with TCS, pre-operative deficits improved after surgery in 14 (58.3%). remained stable in 8 (33.4%). and worsened in 2 (8.3%). Conclusion : The pathologic lesions of tethered cord syndrome in school-aged children, adolescents, and young adults, are mostly intradural lipomas and tight filum. It is suggested that the degree of cord traction results in neurologic dysfunction in late life due to abnormal tension, aggravated by trauma or repeated tugging of the conus during exercise. Early diagnosis and adequate surgical release might be the keys to the successful outcome in school-aged children, adolescents, and young adults with TCS.

Topological and Statistical Analysis for the High-Voltage Transmission Networks in the Korean Power Grid

Seok-Gu Kang,Sung-Guk Yoon 한국통신학회 2017 韓國通信學會論文誌 Vol.42 No.4

Preliminary report about KSPNO-G051 protocol for the treatment of CNS pure germinoma

( Seok Gu Kang ),( Pil Woo Huh ),( Sang Bok Lee ),( Do Sung Yoo ),( Kyoung Souk Cho ),( Dal Soo Kim ),( Chun Kun Park ),( Joon Ki Kang ) 대한임상종양학회 2007 대한임상종양연구 심포지엄 Vol.2007 No.10

Effect of Waterlogging Period on the Growth and Fruit Quality of ‘Campbell Early’ Grapevine

Seok-Beom Kang,In-Bog Lee,Han-Ik Jang,Seung-Heui Kim,Jung-Kwan Park,Yun-Im Kang,Yong-Gu Kang,Jin-Myeon Park 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Tethered Cord Syndrome; Surgical Indication, Technique and Outcome

Kang, Joon-Ki,Lee, Kwan-Sung,Jeun, Sin-Soo,Kang, Seok-Gu,Lee, Il-Woo,Yoon, Kang-Jun,Ha, Sang-Soo The Korean Neurosurgical Society 2007 Journal of Korean neurosurgical society Vol.42 No.2

Objective : The authors tried to reveal some unique features of lipomeningomyelocele (LMMC), including clinical presentation, factors precipitating onset of symptoms, pathologic entities of LMMC associated with tethered cord syndrome, and surgical outcome in LMMC patients. Methods : Seventy-five patients with LMMC were enrolled in this study. Neuro-imaging and intraoperative findings allowed classification of LMMC into three Types. The patients were divided into two groups by age : A (51 patients), from birth to 3 years, and B (24 patients), from 3 to 24 years. For prevention of retethering of the cord, a mega-dural sac rebuilding procedure was performed in 15 patients. Results : During a mean postoperative follow-up period of 4 years, the surgical outcome was satisfactory in terms of improved pain and motor weakness, but disappointing with reference to the resolution of bowel and bladder dysfunction. Among these 75 patients with LMMC, preoperative deficits were improved after surgery in 29 (39%), remained stable in 28 (37%), changed slightly in 13 (17%), and worsened in 5 (7%). Patients in group A achieved better outcomes than those in group B. Depending on the type of lesion, patients with types I and II LMMC have better outcomes than those with type III LMMC. Finally, retethering of the cord with neurological deterioration occurred in 4 (5.3%) of the 75 patients, but no retethering was found in the 15 patients who were recently treated with a mega-dural sac rebuilding procedure. Conclusion : Our data continue to support the opinion that early diagnosis and optimal surgery are still essential for the treatment of patients with LMMC, since there is a high likelihood of residual neurological functions that can be preserved. Based on our surgical experience of untethering and decompression of lipomas, a mega-dural sac repair is useful to prevent retethering of the cord.