Proteomics Analysis of Gastric Epithelial AGS Cells Infected with Epstein-Barr Virus

Ding, Yong,Li, Xiao-Rong,Yang, Kai-Yan,Huang, Li-Hua,Hu, Gui,Gao, Kai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Effects of the Epstein-Barr virus (EBV) on cellular protein expression are essential for viral pathogenesis. To characterize the cellular response to EBV infection, differential proteomes of gastric epithelial AGS cells were analyzed with two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) and liquid chromatography electrospray/ionization ion trap (LC-ESI-IT) mass spectrometry identification. Mass spectrometry identified 9 altered cellular proteins, including 5 up-regulated and 4 down-regulated proteins after EBV infection. Notably 2-DE analysis revealed that EBV infection induced increased expression of heat shock cognate 71 kDa protein, actin cytoplasmic 1, pyridoxine-5'-phosphate oxidase, caspase 9, and t-complex protein 1 subunit alpha. In addition, EBV infection considerably suppressed those cellular proteins of zinc finger protein 2, cyclin-dependent kinase 2, macrophage-capping protein, and growth/differentiation factor 11. Furthermore, the differential expressional levels of partial proteins (cyclin-dependent kinase 2 and caspase 9) were confirmed by Western blot analysis.Thus, this work effectively provided useful protein-related information to facilitate further investigation of the mechanisms underlying EBV infection and pathogenesis.

ZigBee를 이용한 광부 위치 관리 시스템의 설계

정개 ( Ding Kai ),박석천 ( Seok-cheon Park ) 한국정보처리학회 2013 한국정보처리학회 학술대회논문집 Vol.20 No.1

광산의 작업 환경은 매우 위험하기 때문에 안전사고가 빈번히 발생하고 있으며, 이는 광부의 개인 안전을 크게 위협하고 있다. 광부에게 광산에서 안전사고가 생길 시에는 광부의 위치가 제일 먼저 파악되어야 하고 이는 가장 중요한 정보 중 하나이다. 따라서 본 논문에서는 ZigBee를 이용하여 무선 센서 네트워크를 구성하고, 안전사고가 빈번히 발생하는 광산에서의 광부 위치 관리 시스템을 설계하였다. 이를 위해 본 논문에서는 광부의 이동범위에 설치된 ZigBee Reader와 광부의 신체 및 소지품에 부착된 ZigBee Tag와 ZigBee 무선 센서 네트워크의 정보를 관리하는 ZigBee 서버및 데이터를 기록할 DB와 지상 관리자의 요구를 받아 이 정보를 지상 관리자에게 알려주는 위치 관리 시스템을 설계하였다.

A Computer Model for Simulating the Bicycle Rider's Behavior in a Virtual Riding System

( Wei-long Ding ),( Xiao Ding ),( Kai Chen ),( Zang-xin Wan ),( Yan Xu ),( Yuan-jing Feng ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.3

People are increasingly demanding to experience realistic behavior of virtual characters in computer games. In this article, we build a computer model for simulating a bicycle rider’s behavior in a virtual riding system. A method to calculate the speed of a bicycle rider is proposed to improve the reality in a virtual riding system. In this method, the property of physical energy is introduced, and the bicycle-riding speed is calculated in real time according to the relationship between the rider’s physical energy and bicycle-riding speed. Then based on the analysis of the behavior of a cyclist in a real competition, various behaviors of the virtual rider are designed and a behavior-tree for the virtual bicycle rider is constructed accordingly. On the basis of these, a virtual riding system is developed. The experiments results show that our system can simulate the behavior of a virtual bicycle rider, and thus encourage exercise on a stationary bicycle.

CK2 phosphorylates AP-2α and increases its transcriptional activity

( Kai Qun Ren ),( Shuang Lin Xiang ),( Fang Li He ),( Wen Feng Zhang ),( Xiao Feng Ding ),( Yan Yang Wu ),( Li Ping Yang ),( Jian Lin Zhou ),( Xiang Gao ),( Jian Zhang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.7

Transcription factor AP-2α involves in the process of mammalian embryonic development and tumorigenesis. Many studies have shown that AP-2α functions in association with other interacting proteins. In a two-hybrid screening, the regulatory subunit β of protein casein kinase 2 (CK2β) was identified as an interacting protein of AP-2α; we confirmed this interaction using in-vitro GST pull-down and in-vivo co-immunoprecipitation assays; in an endogenous co-immunoprecipitation experiment, we further found the catalytic subunit α of protein casein kinase 2 (CK2α) also exists in the complex. Phosphorylation analysis revealed that AP-2α was phosphorylated by CK2 kinase majorly at the site of Ser429, and such phosphorylation could be blocked by CK2 specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) in a dose-dependent manner. Luciferase assays demonstrated that both CK2α and CK2β enhanced the transcription activity of AP-2α; moreover, CK2β increased the stability of AP-2α. Our data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2α.

ON THE NORM OF THE OPERATOR aI + bH ON L^p(ℝ)

Ding, Yong,Grafakos, Loukas,Zhu, Kai Korean Mathematical Society 2018 대한수학회보 Vol.55 No.4

We provide a direct proof of the following theorem of Kalton, Hollenbeck, and Verbitsky [7]: let H be the Hilbert transform and let a, b be real constants. Then for 1 < p < ${\infty}$ the norm of the operator aI + bH from $L^p(\mathbb{R})$ to $L^p(\mathbb{R})$ is equal to $$\({\max_{x{\in}{\mathbb{R}}}}{\frac{{\mid}ax-b+(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}ax-b-(bx+a){\tan}{\frac{\pi}{2p}}{\mid}^p}{{\mid}x+{\tan}{\frac{\pi}{2p}}{\mid}^p+{\mid}x-{\tan}{\frac{\pi}{2p}}{\mid}^p}}\)^{\frac{1}{p}}$$. Our proof avoids passing through the analogous result for the conjugate function on the circle, as in [7], and is given directly on the line. We also provide new approximate extremals for aI + bH in the case p > 2.

Identification of HDAC9 as a viable therapeutic target for the treatment of gastric cancer

Kai Xiong,Hejun Zhang,Yang Du,Jie Tian,Shigang Ding 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Histone deacetylase inhibitors (HDACis) are a new class of anticancer drugs confirmed to have good therapeutic effects against gastric cancer (GC) in preclinical experiments, but most HDACis are non-selective (pan-HDACis), with highly toxic side effects. Therefore, it is necessary to screen HDAC family members that play key roles in GC as therapeutic targets to reduce toxic side effects. In this study, we evaluated the targeting specificity of the HDACi suberoylanilide hydroxamic acid (SAHA) for GC via fluorescence molecular imaging (FMI). In vitro FMI results showed that SAHA had higher binding affinity for GC cells than for normal gastric cells. In vivo FMI of gastric tumor-bearing mice confirmed that SAHA can be enriched in GC tissues. However, there was also a high-concentration distribution in normal organs such as the stomach and lungs, suggesting potential side effects. In addition, we found that among the HDAC family members, HDAC9 was the most significantly upregulated in GC cells, and we verified this upregulation in GC tissues. Further experiments confirmed that knockdown of HDAC9 inhibits cell growth, reduces colony formation, and induces apoptosis and cell cycle arrest. These results suggest that HDAC9 has an oncogenic role in GC. Moreover, HDAC9 siRNA suppressed GC tumor growth and enhanced the antitumor efficacy of cisplatin in GC treatment by inhibiting the proliferation and inducing the apoptosis of GC cells in vitro and in vivo. Our findings suggest that the development of HDAC9-selective HDACis is a potential approach to improve the efficacy of chemotherapy and reduce systemic toxicity.

Real Time Related Key Attack on Hummingbird-2

( Kai Zhang ),( Lin Ding ),( Junzhi Li ),( Jie Guan ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.8

Hummingbird is a lightweight encryption and message authentication primitive published in RISC`09 and WLC`10. In FSE`11, Markku-Juhani O.Saarinen presented a differential divide-and-conquer method which has complexity upper bounded by 2<sup>64</sup> operations and requires processing of few megabytes of chosen messages under two related nonces (IVs). The improved version, Hummingbird-2, was presented in RFIDSec 2011. Based on the idea of differential collision, this paper discovers some weaknesses of the round function WD16. Combining with the simple key loading algorithm, a related-key chosen-IV attack which can recover the full secret key is proposed. Under 15 pairs of related keys, the 128 bit initial key can be recovered, requiring 2<sup>27</sup> chosen IV and the computational complexity is O(2<sup>27</sup>). In average, the attack needs several minutes to recover the full 128-bit secret key on a PC. The experimental result corroborates our attack. The result shows that the Hummingbird-2 cipher can`t resist related key attack.

High step-up three-level DC-DC converter with three-winding coupled-inductor

Ding, Xinping,Zhao, Delin,Liu, Yun,Li, Kai,Hao, Yangyang The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.1

Due to the wide adoption of photovoltaic power generation, non-isolated high step-up DC-DC converters are becoming more and more popular. Among these converters, the switched/coupled-inductor-based converter is more attractive due to the fact that it has fewer power devices and a high efficiency. This paper presents a three-level high step-up DC-DC topology with a three-winding coupled-inductor. When compared with a conventional switched/coupled-inductor-based converter, a higher efficiency can be achieved, owing to low voltage stresses devices, fewer semiconductors in the power loop, lower current stresses, and ZCS of the main switches. The operating principle of the proposed topology is analyzed in detail via equivalent circuits, where a theoretical analysis is carried out using state space modelling. Experimental results obtained on a 24-300 V, 360 W lab prototype have verified the excellent performance of the proposed DC-DC converter.

A New Technique of Indirect Component Mode Synthesis and Model Test Validation for Truss Bridge Modal Analysis

Kai-liang Lu,Wei Yan,Qiu-yi Ding,Chao Wang 보안공학연구지원센터 2013 International Journal of Hybrid Information Techno Vol.6 No.3

A Modeling Study of the Characteristics and Mechanism of the Westward Coastal Current during Summer in the Northwestern South China Sea

Yang Ding,Xianwen Bao,Zhigang Yao,Cong Zhang,Kai Wan,Min Bao,Ruixiang Li,Maochong Shi 한국해양과학기술원 2017 Ocean science journal Vol.52 No.1

The characteristics and dynamical mechanism of summer-time coastal current over the North South China Sea shelf have been investigated based on a high resolution unstructuredgrid finite volume community ocean model (FVCOM). Modeldata comparison demonstrates that the model describes and explains well the coastal dynamics over the North South China Sea shelf. The coastal current on the North South China Sea shelf is greatly influenced by monsoon and the freshwater discharge of the Pearl River. Strong southwesterly wind drives the coastal current northeastward. However, under weak southwest monsoon, the coastal current west of the Pearl River estuary (PRE) advects toward the southwest, and splits into two parts when reaching east of the Qiongzhou Strait, with one branch entering the Gulf of Tonkin through the Qiongzhou Strait, transporting low salinity water into the Gulf of Tonkin, and the other part flows cyclonic and interacts with the northeastward current around southeast of Hainan Island, forming a cyclonic eddy east of the Qiongzhou Strait. A variety of model experiments focused on freshwater discharge, wind forcing, tidal rectification, and stratification are performed to study the physical mechanism of the southwestward coastal current which is usually against the summer wind. Process-oriented experiment results indicate that the southwest monsoon and freshwater discharge are important factors influencing the formation of southwestward coastal current during summer.