Herpes Simplex Virus Targeting to the EGF Receptor by a gD-Specific Soluble Bridging Molecule

Nakano, Kenji,Asano, Ryutaro,Tsumoto, Kouhei,Kwon, Heechung,Goins, William F.,Kumagai, Izumi,Cohen, Justus B.,Glorioso, Joseph C. Elsevier 2005 MOLECULAR THERAPY Vol.11 No.4

<P><B>Abstract</B></P><P>Herpes simplex virus 1 (HSV-1) enters cells via initial binding of envelope glycoproteins (g) C and B to cell-surface glycosaminoglycans (GAGs) and subsequent membrane fusion involving envelope gD, gB, and gH/gL. Current insights suggest that the fusion process is initiated by interaction of gD with a cognate cellular receptor, such as the widely distributed cell adhesion molecule nectin-1. To redirect the tropism of HSV-1, we have generated a soluble adapter protein (P-V528LH) comprising the gD-binding variable domain of nectin-1 fused to a single-chain antibody (528LH) recognizing the EGF receptor. The adapter molecule enabled HSV-1 entry into naturally nonpermissive CHO cells expressing the human EGF receptor, but not into CHO cells lacking the receptor, and entry was not observed when the antibody portion of the adapter was replaced with an antibody of different specificity. Adapter-mediated entry increased with the viral dose and was nearly as efficient as direct viral entry into nectin-1-bearing CHO cells. Entry depended on viral gD and was diminished in the absence of cellular GAGs. These experiments represent the first demonstration that a soluble molecule can direct HSV infection via a new receptor, supporting the possible utility of this approach for HSV retargeting.</P>

Ssangsŏng Ch’onggwanbu as the Border between Koryŏ and Yuan Dynasty

Nakano Kota(나카노 고우타) 고려대학교 한국사연구소 2013 International Journal of Korean History Vol.18 No.1

1258년부터 1356년까지의 약100년간 존재한 쌍성총관부(雙城摠管府)는 元이 고려 동북부에 세운 지배기구이다. 쌍성총관부는 100년 동안 유지되었다는 특수성과 이성계 가문의 세력기반이라는 정치적 중요성 때문에 연구자들의 주목을 끌어 왔다. 쌍성총관부에 관한 기존연구는 고려와 쌍성의 관계를 검토한 것이 대부분이고 元과의 관계를 충분히 검토하지 않았다. 본고는 쌍성과 元, 그리고 고려와의 관계를 검토함으로써 쌍성총관부가 元과 고려 사이에서 어떻게 존속했는가를 밝히려고 했다. 그 검토를 통해 아래와 같은 결과를 얻었다. 元은 쌍성을 고려의 일부분 아니면 고려와 女眞의 경계지역 정도로 인식하고 자기의 영토로서 그리 중시하지 않았던 것 같다. 元은 금의 공납을 받는 대신 쌍성총관의 지배권을 인정했으나 고려가 금의 공납을 약속하자 元은 고려의 쌍성수복을 묵인했다. 쌍성총관부와 고려의 관계는 원래 적대적이었다. 1287년에 元에서 乃顔 · 合丹의 난이 일어나자 고려는 그 기회를 이용해 쌍성수복을 시도했으나 성공하지 못했다. 그러나 이 난을 계기로 고려의 영향력이 커지고 쌍성 내부 세력들도 고려와의 관계를 중시하기 시작했다. 그 결과 쌍성은 조림 형제의 출사로 볼 수 있듯이 고려에 대해 의존하게 되었다. 쌍성총관부는 元과 고려라는 자기보다 큰 두개 세력을 균형있게 이용함으로써 100년 가까이나 살아남을 수 있었다. 쌍성에서 고려왕에 대해 비교적 자립적인 이성계 세력이 성장할 수 있던 이유도 쌍성총관부의 이런 성격으로 이해할 수 있지 않을까 한다. Ssangs?ng Ch’onggwanbu, which existed for about 100 years between 1258 and 1356, was a governing body established by Yuan in the northeastern part of Kory?. It has drawn the attention of researchers owing to its survival for a century and the political importance as the power base of Yi S?nggye’s family. Most of the previous studies about Ssangs?ng Ch’onggwanbu have focused on the relationship between it and Kory?. This paper sheds light on the relationship between it and Yuan and Kory? in an attempt to see how it continued to exist between those two stronger powers. It appears that Yuan did not attach great importance to Ssangs?ng, viewing it as a part of Kory? or the border between Kory? and the Jurchen. Yuan allowed general superintendents to exert their right of control over the Ssangs?ng area in return for their submittal of part of their gold production to Yuan as tax, but when Kory? recovered Ssangs?ng and promised to continue to submit part of gold production to Yuan, Yuan made no protest. The leadership of Ssangs?ng Ch’onggwanbu took a hostile attitude toward Kory?. In 1287, when Nayan and Qada’an staged a rebellion against Emperor Shizu in Yuan, Kory? tried to take advantage of the situation as an opportunity to recover Ssangs?ng, but in vain. However, Kory? came to exert stronger influence on the area following the rebellion and the people in Ssangs?ng started to attach more importance to the relationship with Kory?. As a result, Ssangs?ng came to rely on Kory?, as shown by Cho Rim and his brother serving in the Kory? officialdom. Ssangs?ng Ch’onggwanbu was able to survive for a century by taking a balanced position toward Kory? and Yuan. Yi S?nggye could build his power there as a faction relatively independent of the Kory? Dynasty apparently thanks to the unique status that Ssangs?ng Ch’onggwanbu maintained.

T7 RNA Polymerases Backed up by Covalently Trapped Proteins Catalyze Highly Error Prone Transcription

Nakano, Toshiaki,Ouchi, Ryo,Kawazoe, Junya,Pack, Seung Pil,Makino, Keisuke,Ide, Hiroshi American Society for Biochemistry and Molecular Bi 2012 The Journal of biological chemistry Vol.287 No.9

Deep desulfurization of gas oil over NiMoS catalysts supported on alumina coated USY-zeolite

Nakano, K.,Ali, S.A.,Kim, H.J.,Kim, T.,Alhooshani, K.,Park, J.I.,Mochida, I. Elsevier Scientific Pub. Co 2013 Fuel processing technology Vol.116 No.-

A series of NiMoS catalysts supported on USY-zeolite coated with different amounts of alumina were prepared and evaluated for deep hydrodesulfurization (HDS) of gas oil using a flow reactor system in single- and dual-bed configurations. The performance of these catalysts was compared to the conventional HDS catalyst as well as to NiMoS catalyst supported on physical mixture of alumina and USY-zeolite. Physically mixed alumina and USY zeolite showed high HDS activity, but it was accompanied with significant cracking of hydrocarbons. Activity and kinetic analysis showed that heavy coating of alumina on the zeolite resulted in much lower HDS and cracking activity. On the other hand, very light coating of alumina on the zeolite resulted in catalytic behavior similar to that of physical mixture of USY-zeolite and alumina. The general trend was an increase in HDS as well as hydrocracking activity with the decrease in amount of alumina coating over zeolite. Changes in product density, aromatic content, mid-boiling point, cetane index, and molecular weight also reflected this trend. The results of this study indicate that catalysts with neither too low nor too high amount of alumina coating on the USY zeolite are suitable to achieve the balance between high HDS and low cracking activities. The highlight of the present study is that it confirmed the deep HDS activity of USY containing alumina supports in the flow reactor system which is industrially applied. In addition, the stability test confirmed the long-term performance of alumina-coated zeolite catalyst.

Research Trends in Japan on the Japanese Invasion of Korea in 1592(Imjin War)

Nakano Hitoshi(나카노 히토시) 고려대학교 한국사연구소 2013 International Journal of Korean History Vol.18 No.2

「朝鮮出兵」, 「文??慶長의 役」 또는 「壬辰倭?」에 대한 연구는 에도(江?)시대로부터 활발하게 진행되었으며, 근대에 한하여도 극히 방대한 학술?연구사를 보유한다. 그러나 1970년대까지는 반드시 충분한 실증연구가 진행되지 않았다. 豊臣政?論의 일환으로서 이론적인 요청에 대응하는 연구가 중심이었다. 80년대에 들어가면 北島万次氏 등에 의해 실증적인 연구가 개척되어, 90년대에는 관심 또한 다양화되고, 戰役에 대한 추이는 물론 兵站補給, 講和交涉, 「倭城」,降倭·義兵·被擄와 같은 조선사회에 관한 여러 문제 등, 전쟁이 가지는 여러 측면에 대해 실증적인 연구가 진행되었다. 다양한 시점에 의한 연구의 심화는 2000년대 이후에도 계속되었다. 본론에서는 70년대까지의 연구를 살펴보고, 80년대 이후에 대해서는 대략 10년마다 시간을 구분하여 연구동향을 서술하였다. Studies on the Japanese invasion of Korea in the late 16th century (known as “The Japanese Disturbance of Imjin” in Korea and “The Korean Campaign” or “The Bunroku Keicho Attack” in Japan) have been carried out actively since the Edo Period. However, it was not until the 1970s that demonstrative studies had been sufficientlycarried out. Most of the studies were carried out in connection with the need for a theoretical supplementation of the history as part of the Theory of Toyotomi Political Power. In the 1980s, historians, including by Manji Kitajima, started demonstrative studies. The demonstrative studies were expanded in the 1990s and included factors such as military supply, negotiations, Japanese fortresses, Japanese POWs, Korean militias/POWs, and matters concerning Korean society, in addition to the military campaign. The studies continued to deepen from diverse perspectives in the 2000s. This focuses on the research about the research that was carried out over the course of a decade, starting in the 1980s and thereafter, after dealing with the period until the 1970s separately.

¹²³I-Labeled oxLDL Is Widely Distributed Throughout the Whole Body in Mice

Nakano, Atushi,Kawashima, Hidekazu,Miyake, Yoshinori,Zeniya, Tsutomu,Yamamoto, Akihide,Koshino, Kazuhiro,Temma, Takashi,Fukuda, Tetsuya,Fujita, Yoshiko,Kakino, Akemi,Kanaya, Shigehiko,Sawamura, Tatsuy 대한핵의학회 2018 핵의학 분자영상 Vol.52 No.2

Purpose Oxidized low-density lipoprotein (oxLDL) plays a key role in endothelial dysfunction, vascular inflammation, and atherogenesis. The aim of this study was to assess blood clearance and in vivo kinetics of radiolabeled oxLDL in mice. Methods We synthesized $^{123}I-oxLDL$ by the iodine monochloride method, and performed an uptake study in CHO cells transfected with lectin-like oxLDL receptor-1 (LOX-1). In addition, we evaluated the consistency between the $^{123}I-oxLDL$ autoradiogram and the fluorescence image of DiI-oxLDL after intravenous injection for both spleen and liver. Whole-body dynamic planar images were acquired 10 min post injection of $^{123}I-oxLDL$ to generate regional time-activity curves (TACs) of the liver, heart, lungs, kidney, head, and abdomen. Regional radioactivity for those excised tissues as well as the bladder, stomach, gut, and thyroid were assessed using a gamma counter, yielding percent injected dose (%ID) and dose uptake ratio (DUR). The presence of $^{123}I-oxLDL$ in serum was assessed by radio-HPLC. Results The cellular uptakes of $^{123}I-oxLDL$ were identical to those of DiI-oxLDL, and autoradiograms and fluorescence images also exhibited consistent distributions. TACs after injection of $^{123}I-oxLDL$ demonstrated extremely fast kinetics. The radioactivity uptake at 10 min post-injection was highest in the liver ($40.8{\pm}2.4%$ ID). Notably, radioactivity uptake was equivalent throughout the rest of the body ($39.4{\pm}2.7%$ ID). HPLC analysis revealed no remaining $^{123}I-oxLDL$ or its metabolites in the blood. Conclusion $^{123}I-oxLDL$ was widely distributed not only in the liver, but also throughout the whole body, providing insight into the pathophysiological effects of oxLDL.

First Record of Orobdella tsushimensis (Hirudinida: Arhynchobdellida: Gastrostomobdellidae) from the Korean Peninsula and Molecular Phylogenetic Relationships of the Specimens

Nakano, Takafumi,Seo, Hong-Yul The Korean Society of Systematic Zoology 2014 Animal Systematics, Evolution and Diversity Vol.30 No.2

Specimens of the genus Orobdella Oka, 1895 from Korea, including various locations in the Korean Peninsula, were identified as Orobdella tsushimensis Nakano, 2011. Phylogenetic analyses using mitochondrial cytochrome oxidase subunit 1 (COI), ND1, $tRNA^{Cys}$, $tRNA^{Met}$, 12S rRNA, $tRNA^{val}$, and 16S rRNA markers show that the newly collected specimens form a monophyletic group with the known O. tsushimensis specimens. The genetic distance of COI of these specimens was in the range 0.4-6.6%. These results confirm that the newly collected specimens belong to O. tsushimensis. This is the first record of the genus Orobdella from the Korean Peninsula.

Recent Advances in Multireference-Based Perturbation Theory

Nakano, Haruyuki,Hirao, Kimihiko Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.6

Accurate ab initio computational chemistry has evolved dramatically. In particular, the development of multireference-based approaches has opened up a completely new area, and has had a profound impact on the potential of theoretical chemistry. Multireference-based perturbation theory (MRPT) is an extension of the closed-shell single reference Møller-Plesset method, and has been successfully applied to many chemical and spectroscopic problems. MRPT has established itself as an efficient technique for treating nondynamical and dynamical correlations. Usually, a complete active space self-consistent field (CASSCF) wave function is chosen as a reference function of MRPT. However, CASSCF often generates too many configurations, and the size of the active space can outgrow the capacity of the present technology. Many attempts have been proposed to reduce the dimension of CASSCF and to widen the range of applications of MRPT. This review focuses on our recent development in MRPT.

Bioavailability of Iron-fortified Whey Protein Concentrate in Iron-deficient Rats

Nakano, Tomoki,Goto, Tomomi,Nakaji, Tarushige,Aoki, Takayoshi Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.7

An iron-fortified whey protein concentrate (Fe-WPC) was prepared by addition of ferric chloride to concentrated whey. A large part of the iron in the Fe-WPC existed as complexes with proteins such as ${\beta}$-lactoglobulin. The bioavailability of iron from Fe-WPC was evaluated using iron-deficient rats, in comparison with heme iron. Rats were separated into a control group and an iron-deficiency group. Rats in the control group were given the standard diet containing ferrous sulfate as the source of iron throughout the experimental feeding period. Rats in the iron-deficiency group were made anemic by feeding on an Fe-deficient diet without any added iron for 3 wk. After the iron-deficiency period, the iron-deficiency group was separated into an Fe-WPC group and a heme iron group fed Fe-WPC and hemin as the sole source of iron, respectively. The hemoglobin content, iron content in liver, hemoglobin regeneration efficiency (HRE) and apparent iron absorption rate were examined when iron-deficient rats were fed either Fe-WPC or hemin as the sole source of iron for 20 d. Hemoglobin content was significantly higher in the rats fed the Fe-WPC diet than in rats fed the hemin diet. HRE in rats fed the Fe-WPC diet was significantly higher than in rats fed the hemin diet. The apparent iron absorption rate in rats fed the Fe-WPC diet tended to be higher than in rats fed the hemin diet (p = 0.054). The solubility of iron in the small intestine of rats at 2.5 h after ingestion of the Fe-WPC diet was approximately twice that of rats fed the hemin diet. These results indicated that the iron bioavailability of Fe-WPC was higher than that of hemin, which seemed due, in part, to the different iron solubility in the intestine.

Measurement of time-dependent CP asymmetries in B0→KS0ηγ decays

Nakano, H.,Ishikawa, A.,Sumisawa, K.,Yamamoto, H.,Adachi, I.,Aihara, H.,Al Said, S.,Asner, D. M.,Aulchenko, V.,Aushev, T.,Ayad, R.,Babu, V.,Badhrees, I.,Bansal, V.,Behera, P.,Beleñ,o, C.,Bhuyan, American Physical Society 2018 Physical Review D Vol.97 No.9

<P>We report a measurement of time-dependent CP violation parameters in B-0 -> K-S(0)eta gamma decays. The study is based on a data sample, containing 772 x 10(6)B(B)over-bar pairs, that was collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e(+)e(-) collider. We obtain the CP violation parameters of S = -1.32 +/- 0.77(stat) +/- 0.36(syst) and A = -0.48 +/- 0.41(stat) f 0.07(syst) for the invariant mass of the K-S(0)eta system up to 2.1 GeV/c(2).</P>