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Cho, I-R,Kaowinn, S,Song, J,Kim, S,Koh, S S,Kang, H-Y,Ha, N-C,Lee, K H,Jun, H-S,Chung, Y-H Nature America, Inc. 2015 Cancer gene therapy Vol.22 No.5
Although H-1 parvovirus is used as an antitumor agent, not much is known about the relationship between its specific tropism and oncolytic activity. We hypothesize that VP2, a major capsid protein of H-1 virus, determines H-1-specific tropism. To assess this, we constructed chimeric H-1 viruses expressing Kilham rat virus (KRV) capsid proteins, in their complete or partial forms. Chimeric H-1 viruses (CH1, CH2 and CH3) containing the whole KRV VP2 domain could not induce cytolysis in HeLa, A549 and Panc-1 cells. However, the other chimeric H-1 viruses (CH4 and CH5) expressing a partial KRV VP2 domain induced cytolysis. Additionally, the significant cytopathic effect caused by CH4 and CH5 infection in HeLa cells resulted from preferential viral amplification via DNA replication, RNA transcription and protein synthesis. Modeling of VP2 capsid protein showed that two variable regions (VRs) (VR0 and VR2) of H-1 VP2 protein protrude outward, because of the insertion of extra amino-acid residues, as compared with those of KRV VP2 protein. This might explain the precedence of H-1 VP2 protein over KRV in determining oncolytic activity in human cancer cells. Taking these results together, we propose that the VP2 protein of oncolytic H-1 parvovirus determines its specific tropism in human cancer cells.
Yun, S.J.,Jun, K.J.,Komori, K.,Lee, M.J.,Kwon, M.H.,Chwae, Y.J.,Kim, K.,Shin, H.J.,Park, S. Pergamon Press 2016 Molecular immunology Vol.75 No.-
<P>Tim-3 is an immunomodulatory protein that is expressed constitutively on monocytes but is induced in activated T cells. The mechanisms involved in the regulation of TIM-3 transcription are poorly understood. In the present study, we investigated whether methylation of the TIM-3 promoter is involved in regulating TIM-3 transcription in T cells, and identified a transcription factor that regulates TIM-3 transcription by associating with the TIM-3 minimal promoter region. Pyrosequencing of the TIM-3 promoter up to 1440 bp revealed 11 hypermethylated CpG sites and 4 hypomethylated CpG sites in human CD4(+) T cells as well as in CD11b(+) cells. Dimethylation of histone H3 lysine 4 (H3K4), a mark of transcriptional activation, was predominantly found in the proximal TIM-3 promoter 954 to 34 by region, whereas trimethylation of H3K9 and H3K27, which are markers of transcriptional suppression, were mostly observed in the distal promoter -1549 to -1048 bp region in human CD4+ T cells and CD11b(+) cells. However, no change in the methylation status of CpG sites and the histone H3 in the TIM-3 promoter was found during induction of TIM-3 transcription in T cells. Finally, AP-1 involvement in TIM-3 transcription was shown in relation with the TIM-3 minimal promoter 146 to +144 by region. The present study defines the minimal TIM-3 promoter region and demonstrates its interaction with c-Jun during TIM-3 transcription in CD4(+) T cells. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Kim, C.J.,Kim, Y.J.,Lim, C.Y.,Jun, B.H.,Park, S.D.,Choo, K.N. The Korea Institute of Applied Superconductivity a 2014 한국초전도저온공학회논문지 Vol.16 No.2
Temperature dependence of magnetic moment (m-T) and the magnetization (M-H) at 5 K and 20 K of the in situ processed $MgB_2$ bulk pellets with/without carbon (C) doping were examined. The superconducting critical temperature ($T_c$), the superconducting transition width (${\delta}T$) and the critical current density ($J_c$) were estimated for ten test samples taken from the $MgB_2$ bulk pellets. The reliable m-T characteristics associated with the uniform $MgB_2$ formation were obtained for both $MgB_2$ pellets. The $T_cs$ and ${\delta}Ts$ of all test samples of the undoped $MgB_2$ were the same each other as 37.5 K and 1.5 K, respectively. The $T_cs$ and ${\delta}Ts$ of the C-doped $MgB_2$ were 36.5 K and 2.5 K, respectively. Unlike the m-T characteristics, there existed the difference among the M-H curves of the test samples, which might be caused by the microstructure variation. In spite of the slight $T_c$ decrease, the C doping was effective in enhancing the $J_c$ at 5 K.
Synthesis of ZSM-5 zeolites using hexamethylene imine as a template: Effect of microwave aging
Jun, J.W.,Ahmed, I.,Kim, C.U.,Jeong, K.E.,Jeong, S.Y.,Jhung, S.H. Elsevier Science Publishers 2014 CATALYSIS TODAY - Vol.232 No.-
Zeolite ZSM-5 can be synthesized from gels containing hexamethylene imine (HMI) as an inexpensive template under adequate conditions. Microwave aging under suitable conditions is essential for the crystallization of ZSM-5 from gels containing HMI. Moreover, adequate reaction conditions (such as Al<SUB>2</SUB>O<SUB>3</SUB>/SiO<SUB>2</SUB>, HMI/SiO<SUB>2</SUB> and H<SUB>2</SUB>O/SiO<SUB>2</SUB> ratios, pHs, reaction times and so on) for ZSM-5 syntheses are also suggested. The obtained ZSM-5, after ion exchange to the proton form, can be applied in acid-catalyzed reactions because the acidity and surface area of the zeolite are similar to those of commercial ZSM-5 zeolite. For example, the H-ZSM-5 can be applied in the dehydration of bioalcohols such as ethanol and n-butanol into olefins or aromatics, quite similar to a commercial H-ZSM-5 zeolite.
Jun, H.K.,Lee, S.H.,Lee, H.R.,Choi, B.K. Cell Press 2012 Immunity Vol.36 No.5
Integrins are cell-surface heterodimeric glycoproteins composed of alpha and beta subunits that mediate cell-cell, cell-extracellular matrix, and cell-pathogen interactions. In this study, we report a specific role of integrin α5β1 in NLRP3 inflammasome activation in macrophages stimulated by Td92, a surface protein of the periodontopathogen, Treponema denticola. The direct interaction of Td92 with the cell membrane integrin α5β1 resulted in ATP release and K<SUP>+</SUP> efflux, which are the main events in NLRP3 activation. This interaction was arginine-glycine-aspartate (RGD)-independent, and Td92 internalization was not required for the activity. An integrin α5β1 antibody and oxATP, an ATP receptor antagonist, inhibited NLRP3 expression, caspase-1 activation, interleukin-1β (IL-1β) secretion, and proIL-1β synthesis, all of which were regulated by NF-κB activation. Therefore, our data has identified the integrin α5β1 as a principal cell membrane receptor for both NLRP3 inflammasome activation and IL-1β transcription by a bacterial protein, which could exaggerate inflammation, a characteristic of periodontitis.
Al_(2)O_(3)-MgO계 캐스터블 내화물의 내침식성 향상
전명곤,연상흠,양정훈,김재준,황규홍,정두화 慶尙大學校 經營行政大學院 2004 工學硏究院論文集 Vol.20 No.-
To improve the mechanical and chemical properties of unfired Al_(2)O_(3) castables which is widely used in metal line of steel-making ladle, the ρ-Al_(2)O_(3) and /or fine MgO was added as a matrix powders and the degree of spinel formation was studied. Because the spinel was formed at the contact areas between Al_(2)O_(3) and MgO particles and the volume of in-situ formed spinel increased more abnormally at the site of Al_(2)O_(3) particles than MgO side, Al_(2)O_(3) aggregates was more recommendable than MgO aggregates. And to compare the degree of spinel formation in the Al_(2)O_(3)-MgO castable refractories, ultrafine SiO_(2) and Al_(2)O_(3) powders were added and their effects on physical properties such as permanent volume expansion and cold crushing strength were examined. For ρ-Al_(2)O_(3) binder, it could be replaced the alumina cement so that the CaO content could be reduced, but low compressive strength and firing shrinkage inhibit it's application to castables. But MgO powders should be added and the finer the MgO powder, the better the residual expansion and in-situ Spinel formation was observed. And due to Spinel formation and dense microstructure, CA_(6) phase would not formed around alumina aggregates during corrosion so that the corrosion resistance was much more increased.