초등학생에서 인두배양과 항-OF 항체 보유율

정현주,김선주,김윤정,맹국영,정현주,김선주,김윤정,맹국영 대한임상병리학회 1998 대한임상병리학회지 Vol.18 No.3

Lipomyces starkeyi KSM 22 Glucanhydrolase 함유 용액의 치은 섬유아세표에 대한 영향

윤현정,정현주,김옥수,김도만 전남대학교 치의학연구소 2002 구강과학 Vol.14 No.3

A novel glucanhydrolase from a mutant of Lipomyces starkeyi KSM 22 has additional amylase activity besides mutanolytic activity and has been suggested as promising anti-plaque agent. It has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 glucanllydrolase are desirable for its application as a dental plaque control agent. In human experimental gingivitis model and 6month clinical trial. mouthrinsing with Lipomyces starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effect was negligible. This study was aimed to evaluate the cytotoxic effect of Lipomyces starkeyi KSM 22 glucanhydrolase on human gingival fibroblasts. Primary culture of human gingival fibroblasts at the 4th to 6th passages were used. Glucanhydrolase solution was made from lyophilized glucanhydrolase powder from a mutant of Lipomyces starkeyi KSM 22 solved in PBS and added to DMEM to the final concentration of 0.5, 1, and 2unit. Cells were exposed to glucanhydrolase solution or 0.1% chlorhexidine and the cells cultured in DMEM with 10% FBS and 1% antibiotics as control. After exposure, the morphological change, cell attachmcnt. and cell activity by MTT assay were evaluated in 0.5, 1.5, 3, 6, 24 hours after treatment. The cell proliferation and cell activity was also evaluated at 2 and 7days after Iminute exposure, twice a day. The cell morphology was similar between the Lipomyces starkeyi KSM 22 glucanllydrolase groups and control group during the incubation periods. while most fibroblasts remained as round cell regardless of incubation time in the chlorhexidine group. The numbers of the attached cells in the glucanhydrolase groups were comparable to that of control and significantly higher than the chlorhexidine group. The numbers of the proliferated cells in the glucanhydrolase groups at 7 days of incubation were comparable to the control group and higher than the chlorhexidine group. The cell activity in glucanhydrolase groups paralleled with the increased cell number by attachment and proliferation. According to these results, Lipomyces starkeyi KSM 22 glucanhydrolase has little harmful effect on attachment and proliferation of human gingival fibroblasts, in contrast to 0.1% chlorhexidine which was cytotoxic to human gingival fibroblasts. Therefore this glucanhydrolase preparation is considered as a safe and promising agent for new mouthwash formula in the near future.

半夏瀉心湯이 CCl4 로 유도된 간중독 흰쥐에 미치는 영향

朱旺錫,朴賢俊,尹炳局,鄭成伊,朴宣東 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

The purpose of this study is to observe the effect of Banhasasimtang on serum reaction in CCI₄ treated rats. In this study the experimental rats divided five group(Normal, Control, Sample A, Sample B and Sample C group) Under the same condition Normal group was fed basal diet and water, Control group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and basal diet for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄0.5㎖/㎏) and fed the Bahasasimtang extract(10㎖/㎏) for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks, Sample B group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks and injected carbon tetrachloride(CCI₄ 0.5㎖/㎏), Sample C group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks. The change of GOT, GPT, γ-GPT, ALP, LDH activity and Bilirubin level in blood serum. The obtained results are summarized as follows : 1. In the change of GOT GPT contents, as compared with control group, sample group was significantly decreased. 2. In the change of γ-GPT contents, as compared wth control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDP contents, as compared with control group, sample group was significantly decreased. 5. In the change of Bilirubin contents, as compared with control group, sample group was significantly decreased.

와동내에 조사된 열의 전도양상에 관한 연구

윤현두,허선,김재곤,이두철,백병주 大韓小兒齒科學會 1998 大韓小兒齒科學會誌 Vol.25 No.3

The purpose if this study was to evaluate temperature change occurred in enamel, dentin and pulp due to the heat from cavity prepration with laser. We made three models had different cavity depth : cavity depth of model Awas 3.52mm, model B was 2.32mm, model C was 1.16mm. We irradiated cavity base with thermal capacity of 30J,100J,300J/㎤s during few seconds and studied the change of temperature in tooth during 10 seconds, and estimated change of thermal capacity by different irradiated site and exposure time. AT 300j/㎤s irradiation for 2 seconds, the temperature of irradiated surface was elevated fast according to irradiated thermal energy during I second, In proportion to continuous exposure time, temperature elevated slowly The surface temperature was 1370℃. After discontinue of thermal irradiation, the heat of irradiated surface was diffused in dentin and pulp and the greatest temperature was made, The greatest temperature was disappeared within 10 seconds The greatest temperature of the inner part of model brought about very severe change by different depth. Temperature in pulp was raised by the greater irradiated energy density and exposure time.

불소농도가 인공우식 범랑질의 재석회화에 미치는 영향

윤현두,백병주,김재곤 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.1

The purpose of this study was to evaluate the remineralized effect of fluoride on artificial carious enamel after topical fluoride application and to certificate the most appropriate fluoride concentration. Artificial demineralized bovine enamel blocks were randomly assigned to four groups, and placed in fluoride, 100, 5,000 and 12,000ppm acidulated phosphate fluoride solution. Sound enamel blocks without artificial carious lesion were used as control group. In this study, the morphological characteristics of treated enamel surface and effect of fluoride concentration on remineralization of artificial carious enamel has been investigated by using scanning electron microscopy, chemical analysis and electron probe microanalysis compared with the effect of various fluoride concentration. The Following results were obtained. 1. The enamel surface treated with 100ppm APF was observed more demineralized surface with lots of holes and loosely bounded a few globules. 5,000ppm APF treatment produced a deposits of numerous spherical globules 0.5∼1㎛ in diameter and distinctive rectangular and cuboidal shaped deposits were formed by 12,000ppm APF treament. 2. Amount of KOH-soluble fluoride on enamel surfaces was increated according to treated fluoride concentration level. In the Ⅳ and Ⅴ group were showed significantly higher than that in the Ⅲ group(p<0.05). 3. After treatment with 5,000 and 12,000ppm APF, a remarkable elevation of the fluoride profile was observed within 30㎛ of the outermost layer and was accompanied simultaneously by a high elevation of the calcium profile. However, specimens treated with 100ppm APF showed only a small elevation of fluoride within whole depth than that in the sound and demineralized enamel specimens. 4. In the Ⅳ and Ⅴ group, Ca/P weight percent ratio were showed higher than that in the Ⅰ, Ⅱ, Ⅲ group in 5 and 15㎛ depth, and fluoride content were showed statistically different than that in the Ⅰ, Ⅱ, Ⅲ group in all depth(p<0.05).

CBT를 이용한 안전한 멀티캐스트 그룹 관리

윤성현,장주만,조원환 高麗大學校附設 컴퓨터科學技術硏究所 1999 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.2 No.-

보다 높은 대역폭에서 안전한 멀티캐스트 통신을 하고자 욕구는 기업, 정부, 그리고 인터넷을 사용하는 모든 단체에서의 고조되고 있고 또한 주요 관심사이다. 멀티캐스트 환경에서 보안성을 제공하기 위해 최근까지 진행되어 오고 있는 연구는 주로 그룹 키 관리 기법에 관한 것들이다. 본 논문에서는 화장성이 우수한 CBT 멀티캐스트 라우팅 프로토콜을 기반으로 안전한 인터넷 멀티캐스트를 위한 CBT기반 그룹 관리 시스템을 제안한다. 본 논문에서 제안하는 기법은 핵심라우터가 키 생성, 키 재구성, 그룹 가입, 탈퇴 등 그룹 관리 뿐 아니라 여러 가지 보안사항을 고려한 기능도 제공해 준다. The need for high bandwidth, very dynamic secure internet multicast communications is increasingly evident in a wide variety of commercial, government, and Internet communities. One of the most recently researches is mainly about the group key management schemes. In this paper, We'll propose a CBT-based group management system with scalable CBT multicast routing protocol. The benefits of this proposed technique are that it offers not only key generation, rekey, group join and leaving but also many functions lated security of multicast group.

가족여행 의사결정에 있어서 부부의 영향력에 관한 연구

윤현주,양광호 공주영상정보대학 1998 논문집 Vol.5 No.-

가족을 소비단위의 하나로 생각할 때, 가족 내에서 소비의 주체는 누구인가에 대한 의문과 가정을 의사 결정자로 생각할 때 주요 결정권자는 누구인가에 대한 의문은 서비스 마케터들에게는 중요한 문제이다. 따라서 본 연구는 가족여행에 있어서 가족구조의 형태에 따라 부부의 영향력에 대해서 연구하였다. 인구통계학적변수와 관광 행동분석에 따른 가족여행시장은 20∼30대의 고졸 및 대졸자로서 소득이 200∼300만원수준이며 초등학교자녀가 있는 가정이 가족여행을 많이 하는 것으로 나타났다. 이 형태의 가족에서는 여행 의사결정항목에 대한 영향력이 높게 나타난 가족구성원은 대체로 부부공동결정의 역할구조를 나타냈다. 가족여행시 의사결정을 할 때 가족구조에 따라서 부부의 영향력에 차이가 있는 지를 분석한 결과는 다음과 같다. 가족구조에 따른 여행제안자의 차이는 가족구조 1, 2는 부부 공동결정이 두드러졌다. 이는 가족을 상대로 여행에 관련된 정보를 제공할 경우 젊은 가족에게는 남편과 부인 모두에게 거의 같은 비중은 두어야 할 것이다. 정보수집에 있어서 부부의 영향력을 조사한 결과, 부부가 공동으로 결정하는 경우가 많았다. 그리고, 여행시기결정에서는 부인의 취업여부에 따라 차이가 있었는데, 부인이 취업을 하지 않은 가정에서는 부부공동결정 중에서도 남편 결정이, 부인이 취업한 가정에서는 부부공동결정에서도 부인에 의한 의사결정이 이루어짐을 알 수 있다. 따라서 취업한 부인을 상대로 한 여행상품을 제공할 수 마케팅전략이 필요함을 알 수 있다. 교통수단결정에서는 가장의 교육수준에 따라서 의사결정에 차이가 있었다. 즉, 가장의 교육수준이 고졸이하인 경우는 교통수단결정에 있어서 남편이 결정을 하는 경향을 보였으며, 대졸이상인 경우는 교통수단결정을 하는 데 있어서 부부가 공동으로 결정하는 경향이 두드러졌다. 여행행위는 단편적인 아닌 연속적인 특성을 가지고 있는데 그 과정을 보면, 최초 단계는 여행욕구에 대한 문제인식에서 출발하여 그 후 두 번째 단계에서는 정보를 탐색하게 된다. 세 번째 단계에 이르면 세부적인 의사결정을 하게 되는데 대부분의 가정에서는 부부를 중심으로 한 공동결정 비율이 높다. 그리고 네 번째 단계에서는 실질적인 관광행동에 참여하고, 마지막 단계에서는 여행경험에 대한 평가를 내리게 된다. 여행상품구매 행위는 일반적으로 고관여상품의 구매와 같이 의사결정 과정이 복잡하고 개입되는 중요변수가 많다. 그러한 결과로 본 논문에서는 가족의사결정과정과 부부의 영향력 관한 여행의사결정을 연구·검토하여 관광의 세분시장에 대한 효율적인 마케팅 전략과 관광시설 및 서비스 공급에 유용한 시사점을 발견하고자 하였다. 가족여행에 대한 관광마케팅을 설정하는 데 있어서 관광시장세분화와 목표시장 선정을 중심으로 관광마케팅을 수립하는 것이 이상적이라 하겠다. 시장세분화는 하나의 시장을 구매자의 특성에 따라 몇 개의 집단으로 분리하는 것으로서, 구분된 집단에 대한 적합한 마케팅믹스전략을 수립하는데 중요한 핵심을 하며, 목표시장선정이란 소비자이나 산업시장 등에서 주요한 핵심지역을 시장세분화에 의해서 선정하는 것을 말한다. 본 연구에서는 인구통계학적 변수 및 여행행동분석 등에 의한 분석이 이루어졌기 때문에 이 두 변수에 의한 시장세분화로서 가족여행자에 대한 관광마케팅전략을 시사하고자 한다. 이렇게 인구통계학적변수와 여행행동분석변수에 의사결정자의 특성에 맞추어 시장세분화가 이루어져야 하겠다. 시장세분화가 이루어진 뒤 목표시장의 설정은 가족여행이라는 상품에 가장 밀접하고 가까운 시장을 선택해야 하며 또 가장 효율성이 높은 시장을 찾아야 한다. 그러므로 가족여행의 의사결정이 대부분 부부공동하의 의사결정에 의하여 이루어지므로 이 특성에 적합한 새로운 관광마케팅전략이 요구된다.

조혈모세포이식 후 발생한 주폐포자층 폐렴에 대한 고찰

주지현,최정현,이동건,백지연,고윤호,이혜정,김세희,신호진,박윤희,박지영,김유진,신완식,김춘추 대한감염학회 2001 감염 Vol.33 No.4

Background : Pneumocytitis cainii pneumonia (PCP) can occur in immunocompromised hosts especially such as AIDS or cancer patients. Although recent research had focused on PCP in AIDS patients, few studies have described the clinical presentations of PCP in recipients of stem cell transplantation (SCT). We evaluated the clinical manifestations of PCP in SCT patients admitted at St. Mary's hospital, Seoul, Korea. Methods : The medical records of 17 PCP patients undergoing SCT between Feb. 1998 and Feb. 2000 were reviewed. The diagnosis of PCP was confirmed through the demonstration of Pneumocytitis cainii via either cytology of brochoalveolar lavage (BAL) or histological technique of lung biopsy. CMV disease and CMV infection were confirmed by BAL culture and antigenemia respectively . Results : Seventeen patients were all recipients of allogeneic SCT and 7 of 17 patients were performed non-sibling SCT. Patients presented with symptoms including brief period (4 ∼23 days) of fever (76%), dyspnea (70%), cough (64%), and signs such as rare(58.8%), Sixteen patients (94%) had been receiving immunosuppressive agent such as cyclosporine A (64%) or Fk506 (35%) without PCP prophylaxis. Eleven patients (64%) were treated with corticosteroid with mean dose of 16 mg/day prednisolone and mean duration of 4.6 months after post-SCT period. Twelve patients were co-infected with CMV. Another co-infected miCroorganisms were Pseudomonas aeruginosa, Mycobacterium tuberculosis, herpes simplex virus, parainfluenza virus, Average duration of treatment with trimethoprim-sulfamethoxazole (TMP/SMX) was 21 ±9 days. Four patients died, and three of them were related with PCP. Conclusion : PCP developed frequently in patients who were taking immunosuppressive drug due to graft versus host disease or were not taking TMP/SMX prophylaxis. High risk patients showing fever, cough, or dyspnea should be considered to take early bronchoscopic intervention for detection of PCP. When treat for PCP, it also be considered to the possibility of coinfection such as CMV. (Korean J Infect Dis 33:273∼279, 2001)

Leukemia 에 대한 증례보고

윤현두,정홍주,백병주 大韓小兒齒科學會 1990 大韓小兒齒科學會誌 Vol.17 No.2

Leukemia is malignant tumor that abnormal cells increase in bone marrow because of abnormality of stem cell and result in anemia, thrombocytopenia, infection & infiltration in other tissue. Leukemia has 5.9% in incidence, following to stomach and uterine cancers and tend to increasing. There are four types of leukemia: acute lymphocytic leukemia, acute myelocytic leukemia, chronic lymphocytic leukemia, chronic myelocytic leukemia. In leukemia, there are swelling of gingiva, bleeding anemia and delayed wound healing in oral cavity. In addition, it is likely to bleeding in ilp, tongue and gingiva, because of decreased platelet. In oral region, candidiasis & ulcer is seen frequently, and secondary infection may occur through the ulcer. Chemotherapeutic agents may result in stomatitis, neuritis, xerostomia in oral mucosa. There is higher incidence in hypodontia, hypoplasia than common case, and there are more tendency in hemorrhage and infection.

흰쥐의 신경병증성 통증에 대한경피신경전기자극과 미세전류신경근자극

이윤주,이충휘,조상현,임중우,남택상 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.3