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복합문화공간의 색채계획 : 수원시 영통구 상업지구를 중심으로
서주환,여화선,최선주,박선영,김옥경,승지현,김홍균 경희대학교 부설 디자인연구원 2007 예술· 디자인학연구 Vol.10 No.1
The recognition on leisure living which have been enjoyed by prestige level from popular persons is now widely spreaded in modern capitalism society and change of the leisure living influenced very much to the society and Industry. The 5 day work per week system expanded to the school and contemporary leisure living of the people is recognised as one culture with various contents. This change of leisure living made a recognition on the need for a space for leisure culture contents, which related to a creation of Complex-culture facility to contain various type of multiple leisure culture. The Complex-culture facility is mainly focused on the entertainment space with increased leisure living, which is consist of important element of the Complex-culture facility as performance space, exhibition, sports and shopping space to construct performance hall, theater, exhibition room, sports center, shopping mall, leisure space and plaza. This study, therefore, will Investigate on the function and value of color, and review on the cases of Complex-cultural facility and then choose a space of cultural element lack. It will recompose in Complex-cultural facility.
Silk Fibroin Microsphere and Its Characterization
Yeo, Joo-Hong,Lee, Kwang-Gill,Lee, Yong-Woo,Kweon, Hae-Yong,Woo, Soon-Ok Korean Society of Sericultural Science 2003 International Journal of Industrial Entomology Vol.6 No.2
Using gel filtration chromatography, high molecular silk fibroin with high purity was obtained and silk flbroin microsphere particles (SFMP) could be simply made by spray dryer method. Also, some of the physicochemical properties of SFMP and morphology were investigated. The average molecular weight of pure silk fibroin protein dissolved in calcium chloride is about 61,500g/㏖ as measured by gel permeation chromatography. SFMP was spherical in shape, and particles, sized average of 2 ${\pm}$ 10 ${\mu}$, were observed by SEM and particle analyzer, respectively. Obtaining microspheres particles by spray dryer method accelerated the transition from the random coil to the $\beta$-sheet structure during spray dryer treatment. It was identified by the basic fourier transform infrared spectroscopy of SFMP. The swelling ratio of SFMP is majorly dependent on the pH of the solution, not on the occurred gelation. The characteristic structure, which might be applicable to immobilization of drugs is superior to other matrix materials for the use of biomaterials with skin affinity.
Yeo, Joo-Hong,Lee, Kwang-Gill,Kweon, Hae-Yong,Woo, Soon-Ok,Han, Sang-Mi,Kim, Sung-Su,Demura, Makoto Wiley Subscription Services, Inc., A Wiley Company 2006 Journal of applied polymer science Vol.102 No.1
<P>Fractionated components of Bombyx mori silk fibroin, which were hydrolyzed with protease, were prepared with a preparative-recycling high-performance-liquid-chromatography system to evaluate the protective effects of molecular-weight-controlled B. mori silk fibroin components on H<SUB>2</SUB>O<SUB>2</SUB>-injured neuronal cells. Three major fractions, having molecular weights less than about 1500, were first collected with the recycling techniques. The highest protective effect of the molecular-weight-controlled B. mori silk fibroin components on H<SUB>2</SUB>O<SUB>2</SUB>-injured neuronal cells was obtained when a fraction with a molecular weight of approximately 1400 was used. This protective effect of the silk fibroin hydrolysate on H<SUB>2</SUB>O<SUB>2</SUB>-injured neuronal cells was suggested to correlate with the contents of aromatic amino acids such as tyrosine and phenylalanine. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 772–776, 2006</P>
Synnema formation of Paecilomyces tenuipes using blue LED lamp
Joo-Hong Yeo,Sang-Hoon Han,Kwang-Gill Lee,HaeYong Kweon,You-Young Jo,Ji-Young Mun,Heui-Sam Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
To synnema formation and entomopathogenic of Paecilomyces tenuipes using some of media under the blue LED lamp. Lyophilized matured silkworm hemolymph and Bombyx mori powder were used as nutrient source in cultivation of an entomopathogenic fungus, Paecilomyces tenuipes. Matured silkworm (MS, aged seventh days of fifth instars) was in whole lyophilized without any treatment. Hemolymph (HL) powder was prepared by water-soluble extraction from matured silkworms which was silk gland removed and also lyophilized, too. Both of media showed better growth rates and synnemata formations than potato dextrose (PD) medium, and B. mori was controlled under the blue LED lamp conditions. To investigate their effect on mycelial growth of P. tenuipes, each of them were provided as nutrient sources by final concentrations of 2, 3 and 4%, respectively. Total 18 test cultures were cultivated for 14 days with no light. Three PD cultures were used as control group. Each of growth areas was measured with an image analyzer. The result showed that MS and HL medium promoted mycelial growth of P. tenuipes by 287 and 310%, respectively, in comparison with PD medium. The synnema formation and its shape of Paecilomyces tenuipes using B. mori under the blue LED lamp group are not so good than normal conditions. But, how to change of a compositions are not yet. It will be investigation in the future.
Studies on PVA/Chitosan/Fibroin Blend Sponge Sheets: Preparation and Wound Healing Effects in Rats
Yeo, Joo-Hong,Lee, Kwang-Gill,Lee, Heui-Sam,Lee, Yong-Woo,Kim, Sun-Yeou Korean Society of Sericultural Science 2000 International Journal of Industrial Entomology Vol.1 No.1
The preparation of PVA/Chitosan/Fibroin (PCF)-blended sponge sheets and wound healing effects of these sheets in rats were investigated. We excised the skin off rat, including the dermis, approximately 2${\times}$2 cm in size. The wound was coveted with PCF-blended spongy sheets. The spongy sheets absorbed the exudate, and gained flexibility and softness. Histopathological inspection of the wound 12 days later showed the increase of a vascular ingrowth and the absence of inflammatory cells. Regeneration of the skin around the wound was faster than that of the control. We also tested wound healing effects of PVA, Chitosan and Fibroini alone of in various combinations. Wound healing was accelerated in the order of PVA/chitosan/Fibroin (PCF)-blended sponge>Chitosan/Fibroin (CF)-blended sponge$\geq$Fibroin (F) sponge>PVA/Chitosan-blended sponge (PC)>Chitosan (C) sponge.
여주홍 ( Joo Hong Yeo ),이광길 ( Kwang Gill Lee ),우순옥 ( Soon Ok Woo ),권해용 ( Hae Yong Kweon ),한상미 ( Sang Mi Han ),박경호 ( Kyung Ho Park ),김성수 ( Sung Su Kim ) 한국잠사학회 2007 한국잠사곤충학회지 Vol.49 No.2
실크단백질 식이공급이 동물대장암에 미치는 효과를 알아본 결과 다음과 같은 결과를 얻을 수 있었다. 1. 동물 대장암에 투여한 식이공급용 선발 실크단백질의 피브로인 및 세리신 단백질은 각각 Mw 65,000 내외의 피브로인 및 Mw 100,000만 내외의 세리신 단백질이며, 동물사료의 경우, 섭식 등 물성 등이 작년대비 개선된 3% 첨가된 전용 사료를 조제하여 섭식하도록 하였다. 2. 1,2-DMH에 의한 동물 대장암 유발(1회/1주, 4회 주사) 후 6주 시료 투여에 있어서 대장암 유발군(CA)의 경우, 체중변화 및 식이 섭취량의 관계인 식이효율은 세리신 섭취군(S)의 경우 정상군(C)의 85% 수준을 나타내었다. 3. 동물대장암 조직의 조직관찰에 있어서는 대장암 조직의 COX-2 발현을 염색한 결과, 대장암 유도군(CA)의 경우 확실한 COX-2에 의한 발현을 나타내었으며, 세리신 섭취군(S)의 경우는 그 정도가 미비함을 나타내어 대장암에 효과가 있음을 나타내었다. 4. 대장암 인자변화를 정량적으로 확인한 결과, 정상군(C)에 비하여 대장암 유발군(CA)은 확실한 7배 정도의 COX-2량이 확인되었으며, 세리신 섭취군(S)의 경우는 유발군(CA) 대비 1/2 이하 수준으로 줄어듬을 확인할 수 있어서 금후 대장 질환 관련 소재 개발 가능성을 나타내었다. This study was done for the dietary effect of silk protein, molecular controlled silk sericin and silk fibroin, on colon cancer of animal. The ICR mice were given weekly injections of 1,2-dimethylhydrazine (DMZ) for the initial 10 mg/kg with four weeks, and fed a diets supplemented with 3% (v/v) molecular controlled sericin and fibroin for 6 weeks, respectively. Then, the body weight, efficiency of dietary, typical histologic appearance of animal colon tissue and COX-2 effect were studied, too. Molecular controlled sericin protein are shown more higher dietary effect on animal colon cancer than fibroin supplemental case. The results suggest a potential usefulness of sericin as a chemopreventive for colon carcinogenesis and the development functional food.