Heat-Killed Lactiplantibacillus plantarum APSulloc 331261 Enhances Lipid Catabolism Through AMPK Activation in HepG2 Cells

Hyun Woo Jeong,Donghyun Cho,Jonghwa Roh,Wangi Kim 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4

Fatty liver disease, a condition where excess triglyceride accumulates in the liver, has a high risk that develops into hepatic disorders such as cirrhosis and ultimately hepatic failure. Therefore, it is important to prevent the formation and progression of fatty liver for maintaining a healthy life. Recently, we identified Lactiplantibacillus plantarum APSulloc 331261, a novel strain of L. plantarum from the green tea (Camelia sinensis) field in Jeju Island and found a distinct property differed from other L. plantarum strains. In the present study, attempts were made to assess its potential as an anti-obesity agent by examining effects of heat-killed L. plantarum APSulloc 331261 (GTB1™) on lipid metabolism in the HepG2 cells, a human hepatoma cell line. The results were as follows. In the HepG2 human hepatoma cell line treated with oleic acid, GTB1™ was shown to prevent the accumulation of triglyceride, suppress the expression of lipogenic genes and stimulate the expression of lipid-catabolic genes as well as enhance the mitochondrial contents. In the same cells treated with ethanol, GTB1™ was observed to suppress oxidative stress and cytotoxicity. Interestingly, GTB1™ was shown to activate AMP-activated protein kinase (AMPK). The above effects of GTB1™ observed in HepG2 cells were reduced in the cells treated with an AMPK inhibitor and the cells transfected with AMPK-targeting siRNA. Regarding the pivotal role of AMPK in lipid metabolism, it is likely that GTB1™ exerts its action through the modulation of AMPK activity. All the in vitro results suggest that GTB1™ has a potential as an agent that help manage obesity and fatty liver.

AP 콜라겐 효소분해 펩타이드 섭취가 피부 주름, 탄력 및 보습 개선에 미치는 효과

정경미(Kyoungmi Jung),양미숙(Mi-Suk Yang),김승훈(Seung Hun Kim),노종화(Jonghwa Roh),김완기(Wangi Kim) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.5

본 연구에서는 40~60세 성인 여성을 대상으로 APCP의 피부 주름, 탄력 및 보습 개선에 대한 이중눈가림, 무작위배정, 대조식품 비교 인체적용시험을 진행하였다. 눈가 주름 부위의 육안평가 및 Visiometer를 이용한 피부 주름 개선 평가에서 APCP 1,000 mg 및 1,500 mg 섭취군 모두 12주에 대조식품 섭취군 대비 유의적인 개선 효과를 보였고, 섭취종료 후 2주까지 피부 주름 개선이 유지됨을 확인할 수 있었다. 피부 탄력 개선 평가에서 R2, R5 및 R7 지표에 대해 APCP 1,000 mg 및 1,500 mg 섭취군 모두 6주부터 대조식품 섭취군 대비 유의적인 개선 효과를 보였고 12주까지 통계적으로 유의한 차이를 나타내었다. 피부 보습 개선 평가에서도 APCP 1,000 mg 및 1,500 mg 섭취군 모두 12주에 대조식품 섭취군 대비 유의적인 개선 효과를 보였고 섭취종료 후 2주까지 피부 보습 개선 상태가 유지되었다. 연구를 진행하는 동안 이상반응, 임상실험실 검사 및 활력징후 검사에서 유의미한 변화는 나타나지 않아 APCP의 안전성을 확인할 수 있었다. 본 인체적용시험 결과를 통해 APCP는 피부주름, 거칠기, 탄력 및 보습과 같은 피부 건강 개선에 도움을 줄 수 있는 건강기능식품임을 확인하였다. Collagen is a component of the human body, known to have a beneficial effect on human health when consumed as a food or dietary ingredient. This double-blinded, randomized, placebo-controlled study aimed to evaluate the efficacy and safety of AP collagen peptides (APCP) containing 3% glycine-proline-hydroxyproline, on improving skin wrinkles, elasticity and hydration. Totally, 105 subjects with crow’s-feet wrinkles were randomly assigned to receive either placebo, APCP 1,000 mg or APCP 1,500 mg, once daily for 12 weeks. Efficacy endpoints of skin wrinkles, elasticity and hydration were measured at baseline, 6 and 12 weeks, and at the 2-week follow-up visit. Compared to the placebo group, the visual evaluations and R3 (average roughness) values of crow’s-feet wrinkles were significantly decreased in both APCP groups at 12 weeks, and these effects were maintained until 2 weeks after completion of ingestion. During the intake period at 6 and 12 weeks, three parameters for skin elasticity (R2, R5, R7) showed significant improvement in both APCP groups, as compared to the placebo group. The APCP groups also showed a statistically significant difference in skin hydration compared to the placebo group, at 12 weeks and at 2 weeks after the end of intake. No adverse effects were encountered during the study period. These results indicate that APCP 1,000 mg and 1,500 mg can be used as a functional food ingredient to improve human skin conditions related to skin aging and hydration.

Case Report : Prenatal diagnosis of congenital mesoblastic nephroma

( Ayoung Do ),( Jungsun Kim ),( Sukjoo Choi ),( Sooyoung Oh ),( Cheongrae Roh ),( Jonghwa Kim ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.5

Congenital mesoblastic nephroma is a rare renal tumor that is diagnosed during pregnancy and is associated with polyhydramnios, prematurity, and neonatal hypertension. Differential diagnoses include Wilms tumor, adrenal neuroblastoma, and other abdominal tumors. We report a case of congenital mesoblastic nephroma detected by prenatal ultrasonography as a large fetal renal mass with polyhydramnios at 32 weeks of gestation. Ultrasonography showed a 6×6-cm complex, solid, hyperechoic, round mass in the right kidney. At 35 weeks of gestation, the patient was admitted with preterm premature rupture of membranes and the baby was delivered vaginally. Postnatal ultrasonography and computed tomography showed a heterogeneous solid mass on the right kidney. At the end of the first week of life, a right nephrectomy was performed and subsequent pathological examination confirmed a cellular variant of congenital mesoblastic nephroma with a high mitotic count. Postoperative adjuvant chemotherapy was administered. The newborn was discharged in good condition.

Risk of cesarean section after induced versus spontaneous labor at term gestation

( Hyeran Lee ),( Mina Kim ),( Jiyeon You ),( Sukjoo Choi ),( Sooyoung Oh ),( Cheongrae Roh ),( Jonghwa Kim ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.5

To investigate whether the cesarean section (CS) rate is increased in women whose labor was induced compared to thosewho had spontaneous labor at term pregnancy.A retrospective study was performed in women whose labor was either induced (induction group, n=497) or spontaneous (spontaneous group, n=878) at 37+0 to 41+6 weeks of gestation from January 2008 to June 2009. Maternal age, parity, body mass index (BMI), Bishop scores, gestational age, hypertension, diabetes, delivery mode, indications for CS, neonatal outcome were compared between the two groups. Multiple logistic regression analysis was used to examine the association between the CS rate and labor induction after adjusting for potential confounding variables.CS (17.3% vs. 5.3%, P<0.001) and vacuum-assisted delivery (10.7% vs. 6.4%, P<0.001) rates were significantly higher in the induction group compared to the spontaneous group. The CS rate in the induction group was higher than the spontaneous group not only in nulliparous women (25.3% vs. 8.6%, P<0.001), but also in multiparous women (3.8% vs. 0.3%, P=0.002). However, after adjusting confounding factors, the higher CS rate was significantly associated with advanced maternal age, higher BMI, lower Bishop scores and nulliparity, with no demonstrable tie to labor induction. Neonatal outcome in the two groups were comparable.Although CS rate was higher in women whose labor was induced than those who had spontaneous labor, this higher rate was associated with maternal age, BMI, Bishop scores and parity, but was not impacted by labor induction per se.

Oral Ingestion of AP Collagen Peptide Leads to Systemic Absorption of Gly-Pro-Hyp, Alleviating H2O2-Induced Dermal Fibroblast Aging

Minjung Chae,Chang Yong Moon,Sung-Hwan Lim,Yusuke Yamashita,Hiroaki Yamada,Masahiro Ide,Chan Woong Park,Jonghwa Roh,Wangi Kim 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.5

Collagen-derived dipeptides and tripeptides have various physiological activities. In this study, we compared the plasma kinetics of free Hyp, peptide-derived Hyp, Pro-Hyp, cyclo(Pro-Hyp), Hyp-Gly, Gly-Pro-Hyp, and Gly-Pro-Ala after ingestion of four different collagen samples: AP collagen peptide (APCP), general collagen peptide, collagen, and APCP and γ-aminobutyric acid (GABA) combination. Each peptide was measured by high-performance liquid chromatography and triple quadrupole mass spectrometer. We found that, among all the peptides that were analyzed, only Gly-Pro-Hyp was significantly increased after ingestion of APCP compared with that of general collagen peptides and collagen. In addition, ingestion of the APCP and GABA combination improved the absorption efficiency of Gly-Pro-Ala. Finally, we reveal that Gly-Pro-Hyp was effective for preventing H2O2-induced reduction in extracellular matrix (ECM)-related genes, COL1A, elastin, and fibronectin, in dermal fibroblasts. Taken together, APCP significantly enhances the absorption of Gly-Pro-Hyp, which might act as an ECM-associated signaling factor in dermal fibroblasts, and the APCP and GABA combination promotes Gly-Pro-Ala absorption. Clinical Trial Registration number: UMIN000047972.

Clinical Study on the Effects of AmorePacific Green Tea Extract (AP GTE) on Postprandial Blood Glucose and Insulin after a High-Fat/High-Carbohydrate Meal

강재헌,Nam Hyun-Jin,Jung Kyoungmi,Choi Gyeyoung,Lee Ji-Hae,Jeong Hyun Woo,Roh Jonghwa,Kim Wan-Gi 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.3

Increasingly westernized eating habits and insufficient physical activity have increased prevalence of type 2 diabetes in Korea. Green tea extracts (GTE) obtained from Camellia sinensis have shown beneficial effects particularly on glucose metabolism and transport. Thus, many studies have been done on GTE for its actions on glucose control and insulin sensitivity. However, most of them were performed on cells and animals and not so many were on humans. Therefore, we conducted an open-label, crossover clinical study using AP GTE provided by AmorePacific Corporation R&D Center and investigated its effects on postprandial blood glucose after a high-fat/high-carbohydrate meal. Twenty subjects (men and women) aged ≥ 19 years with a body mass index (BMI) of 18.5–29.9 kg/m2 and fasting glucose of 100–139 mg/dL were enrolled. First, the control study was performed, where all subjects were fasted for 12 hours and blood (35 mL) was collected before (0 hours) they consumed a high-fat/high-carbohydrate meal and at 0.5, 1, 2, 3, 4, and 5 hours after the meal. After at least a week of washout period, AP GTE study was performed in the same way but taking 4 tablets of AP GTE after ingesting the same high-fat/high-carbohydrate meal. The blood samples were used for assessment of glucose and insulin concentrations. AP GTE-treated subjects had significantly lower plasma concentrations of glucose and insulin over 5 hours than the subjects not treated with AP GTE. During the experiment, no adverse effects were observed from the clinical laboratory testing, vital signs, and physical examinations. These results suggest that AP GTE supplementation may give beneficial effects to subjects who need glycemic control.

Effect of Phenylalanine on Differentiation of Myoblast C1C12 and L6 Cells Into Myocytes

Hyun Woo Jeong,Jong Hyun Kim,Chan-Su Rha,Sohn Jonghee,Wangi Kim,Jonghwa Roh 건강기능식품미래포럼 2023 건강기능식품미래포럼 학술지 Vol.3 No.4

In the present study, we aimed to find any essential amino acids (EAAs) that can promote muscle protein synthesis. For this purpose, we examined how the removal of each EAA from the differentiation-inducing medium (Diff.) that is the Dulbecco’s Modified Eagle’s Medium with 2% horse serum affects the differentiation of C2C12 (mouse myoblasts) or L6 (rat myoblasts) cells into mature myocytes. This method was chosen because muscle protein synthesis is significantly enhanced during the differentiation process. This rationale was confirmed by the observation that C2C12 cells showed increased expressions of myosin heavy chain when induced to differentiate by culturing them in the Diff.. When we induced the C2C12 or L6 cells in the Diff. with each EAA depletion, we found that their differentiation rate and the expression of myogenic marker proteins (MyoD and MyoG) was blunted. This decreases were most noticeable when cultured in the Diff. with phenylalanine removed (Diff.-Phe). This effect was comparable to that of leucine. Further, the phenylalanine-removing effect could be recovered by supplementing this EAA back to the Diff.-Phe but not by adding tyrosine, indicating that the effect on differentiation is due to phenylalanine itself. These findings suggest that phenylalanine plays a role in the differentiation of myoblasts, particularly when muscle protein synthesis is enhanced, implying that phenylalanine could contribute to the promotion of muscle protein synthesis. Based on these findings, it is expected that phenylalanine, like leucine, could provide benefits in preventing the loss of muscle mass.

AP 콜라겐 효소분해 펩타이드의 세포외기질 단백질 생성 및 피부 치밀도 개선 효과

정경미(Kyoungmi Jung),채민정(Minjung Chae),김승훈(Seung Hun Kim),김아름(A Reum Kim),임성환(Sung-Hwan Lim),문창용(Changyong Moon),노종화(Jonghwa Roh),김완기(Wangi Kim) 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.2