신뢰성 기준을 적용한 용수공급능력의 해석 : 낙동강유역을 중심으로 for the Nakdong River Basin

차상화,지홍기,이순탁 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

In general, the evaluation of water supply capacity is important factor to establish various establishment of water resource supply plan include water resource security and determination of dam's mass. But former researchs about estimation of water supply capacity were lack in continunity of evaluation basis, and didn't excute analysis on reliability criteria also. In this study, Nakdong river was selected for study basin, and then water supply capacity was analyzed by HEC-5 model using indentical reliability criteria.

주파수 도약 대역 확산 통신 방식의 동기 시스템 구현에 관한 연구

方孝昌,權智洪,張太和,金元厚 한국항공대학교 1992 論文集 Vol.30 No.-

주파수 도약 대역 확산 통신 방식에서 기존의 동기 시스템은 VCO의 비선형 특성, 루프의 온도보상문제, 소형화. 경량화 문제 등으로 시스템 구현에 많은 어려움을 겪고 있었다. 본 연구에서는 정합필터(matched filter)를 이용한 초기동기와 ADPLL(All Digital Phase Locked Loop)을 이용한 동기 추적 방식의 동기 시스템을 구현하였고, 초기동기시간 약 600μS, 동기추적에 약46mS의 시간을 얻어 전체 동기 시스템은 약 65mS이내를 얻었다. 일반적으로 동기시간이 100mS 이내에 이루어지면 완전한 동기를 이룬것으로 보며, 따라서 구현된 동기 시스템이 매우 안정되고 정확한 동기를 이루고 있음을 알 수 있었다. In the frequency hopping spread spectrum communication, the conventional synchronization systems have many problems in their implementation. It is nonlinear characteristics of VCO, temperature compensation of loop and implementing compactly. In this paper, we implemented the synchronization system which consists of the ACQUISITION by matched filter and the TRACKING by ADPLL(All Digital PLL). In this synchronization system, acquisition time and tracking time were about 600㎲, 64ms respectively, so that total synchronization time could be within 65ms. Generally, as synchronization time is within 100ms we can regard it as complete synchronization. therefore, we could prove that the implemented synronization system has a stable and accurate synchronization.

Database Construction for Variety Characteristics of Lettuce (Lactuca sativa L.) using SSR Markers

Jee-Hwa Hong,Yong-Sham Kwon,Kyoung-In Seo,Moo-Kyoung Yoon 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Seed & Variety Service for Plant Variety Protection (PVP). We previously constructed DNA profile database for identifying 159 lettuce varieties using 60 simple sequence repeat (SSR) markers. In this study, we selected optimum markers from previously applied markers and new SSR markers for the standardization of DNA profile database of 65 commercial lettuce varieties containing 18 PVP varieties distributed in Korea. Twenty-eight SSR markers from 60 SSR markers were selected for characterization with 65 commercial lettuce varieties according to the reproducibility, polymorphism, band pattern of marker and easiness of scoring. Out of 156 SSR primers, we additionally selected 11 new SSR primer pairs showed polymorphisms between 8 varieties and repetitive reproducibility on capillary electrophoresis system. Totally 127 polymorphic amplified fragments were obtained using 39 SSR markers. Two to seven SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Average polymorphism information content was 0.517, ranging from 0.281 to 0.771. Genetic distance of clusters ranged from 0.29 to 0.96 by unweighted pair-group method with arithmetical average based on Jaccard’s distance coefficients. A total of 65 commercial lettuce varieties were discriminated by 39 SSR marker genotypes. These SSR profile database developed will be continually characterized for the standardization of DNA profiles for lettuce commercial varieties

Species-specific QTLs Associated with Anthracnose Resistance to Different Colletotrichum spp. in an Introgressed BC1F2 Population from Capsicum annuum×C. baccatum

Jee Hwa Hong,Jae Wahng Do,Hyo Guen Park,Jae Bok Yoon 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Development of Novel SSR Markers using NGS sequencing and Genetic Relationship Analysis in Blueberry (Vaccinium spp.)

Jee-Hwa Hong,Eun-Jo Shim,Moo-Kyoung Yoon,Eun-Hee Soh 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Blueberry (Vaccinium spp.) is a member of the Ericaceae and eleven varieties have been registered at the Korea Seed & Variety Service for Plant Variety Protection (PVP). This study was to develop simple sequence repeat (SSR) markers next generation sequencing (NGS) analysis and to analysis genetic relationship of blueberry 31 varieties. Highbush blueberry ‘Camellia’ and rabbiteye blueberry ‘Alapaha’ varieties were used as sequencing materials. Out of total 987 SSR primers detected between ‘Camellia’ and ‘Alapaha’, 148 SSR primers were initially applied to select SSR markers for identification of blueberry varieties. Fourteen SSR markers showed polymorphism between 8 varieties. Seven SSR markers showed reproducibility and clear peak among 14 SSR markers. Genetic relationships of 31 blueberry varieties were analyzed and identified using 7 SSR markers. A total of 30 polymorphic SSR alleles were obtained and two to seven alleles were detected for each locus with an average of 4.3 alleles per locus. Average polymorphism information content was 0.556, ranging from 0.374 to 0.714. Genetic distance of clusters ranged from 0.38 to 0.93 by unweighted pair-group method with arithmetical average based on Jaccard’s distance coefficients. These newly developed SSR markers indicate usefulness for variety identification related to seed dispute and distinctness, uniformity and stability (DUS) test for blueberry.

A Common Major QTL for Anthracnose Resistance in Two Different BC₁F₂ Populations Raised from an Interspecific Cross of Capsicum annuum × C. baccatum

Jee Hwa Hong(홍지화),Jae Wahng Do(도재왕),Dong Cheol Yang(양동철),Jae Bok Yoon(윤재복),Hyo Guen Park(박효근) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.10

EST로부터 개발된 SSR 마커를 이용한 상추 유전자원 및 유통품종의 식별

홍지화(Jee-Hwa Hong),권용삼(Yong-Sham Kwon),최근진(Keun-Jin Choi),Raghvendra Kumar Mishra,김두환(Doo Hwan Kim) 한국원예학회 2013 원예과학기술지 Vol.31 No.6

본 연구의 목적은 상추(Lactuca sativa)의 expressed sequence tag(EST)로부터 simple sequence repeat(SSR) 마커를 개발하고, 개발된 EST-SSR 마커를 이용하여 상추의 3가지 야생종의 유전자원 9점과 61개의 유통품종을 식별하는 것이다. NCBI 데이터베이스로부터 총 81,330개의 상추 EST를 대상으로 SSR을 탐색하였고, 총 4,229개의 SSR을 발견하였다. SSR의 반복 motif 중 trinucleotide(59.12%, 2,500개)가 가장 많았고, 그 다음으로 dinucleotide(29.70%, 1,256개), hexanucleotide(6.62%, 280개) 순의 분포를 나타내었다. EST로부터 총 474개의 EST-SSR primers를 개발하였고, 이 중 267개의 primer를 9점의 유전자원과 61품종에 대한 유전적 다양성 평가에 활용하였다. 267개의 마커 중 47개의 EST-SSR 마커가 7개 품종 내에서 다형성을 보였으며, 이 중 다형성 정도와 반복 재현성 및 밴드의 선명성을 고려하여 26개의 EST-SSR 마커를 선발하였다. 최종 선발된 26개의 SSR 마커를 이용하여 70개 공시재료를 분석한 결과 대립유전자 수는 총 127개였으며, 최소 2개에서 9개의 분포를 나타내었으며 마커당 평균 대립유전자 수는 4.88개를 나타내었다. PIC 평균값은 0.542로 나타났으며, 0.269-0.768의 범위를 나타내었다. 70개 공시재료의 유전적 거리는 0.05-0.94로 나타났으며, 유사도 지수 0.34를 기준으로 할 때 7개의 주요 그룹으로 나누어졌다. 26개의 EST-SSR 마커를 이용한 유전적 다양성 분석 결과 9점의 유전자원과 61개의 유통품종이 마커의 유전자형에 의해 모두 식별이 되었다. 본 연구를 통해 신규 개발된 EST-SSR 마커는 상추의 품종식별과 구별성, 균일성, 안정성 검정에 유용하게 활용될 수 있을 것으로 사료된다. The objective of this study was to develop simple sequence repeat (SSR) markers from expressed sequence tags (EST) of lettuce (Lactuca sativa) and identify 9 germplasms from 3 wild species of lettuce and 61 commercial cultivars using the developed EST-SSR markers. A total of 81,330 lettuce ESTs from NCBI databases were used to search for SSR and 4,229 SSR loci were identified. The highest proportion (59.12%, 2500) was represented by trinucleotide, followed by dinucleotide (29.70%, 1256) and hexanucleotide (6.62%, 280) among SSR repeat motifs. Totally 474 EST-SSR primers were developed from EST and a random set of 267 primers was used to assess the genetic diversity among 9 germplasms and 61 cultivars. Out of 267 primers, 47 EST-SSR markers showed polymorphism between 7 cultivars. Twenty-six EST-SSR markers among 47 EST-SSR markers showed high polymorphism, reproducibility, and band clearance. The relationship between 26 markers genotypes and 70 accessions was analyzed. Totally 127 polymorphic amplified fragments were obtained by 26 EST-SSR markers and two to nine SSR alleles were detected for each locus with an average of 4.88 alleles per locus. Average polymorphism information content was 0.542, ranging from 0.269 to 0.768. Genetic distance of clusters ranged from 0.05 to 0.94 between 70 accessions and dendrogram at a similarity of 0.34 gave 7 main clusters. Analysis of genetic diversity revealed by these 26 EST-SSR markers showed that the 9 germplasms and 61 commercial cultivars were discriminated by marker genotypes. These newly developed EST-SSR markers will be useful for cultivar identification and distinctness, uniformity and stability test of lettuce.

분만기 주위 응급 자궁적출술의 임상적 고찰

지홍련(Hong Lyon Jee),박시홍(Si Hong Park),이경화(Kyung Hwa Lee),윤병철(Byung Chul Yoon),김중근(Jung Geun Kim) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12

N/A Objective: Peripartum emergency cesarean hysterectomy of 28 cases at our hospital for 10years were reviewed that the clinical indication, history, and annual incidence change. Methods: A retrospective descriptive analysis from January, 1988 to December, 1997 was carried out. Results: There were 28 cases of emergency cesarean hysterectomy identified during this period among total 24,689 deliveries. The annual incidence of emergency cesarean hysterectomy was significantly declined statistically(p<0.005). Cesarean hysterectomy was performed in 20 of 6,671 cesarean section(0.30%) and in 8 of 18,018 vaginal deliveries(0.04%), so more frequently after cesarean section than vaginal delivery. The cesarean hysterectomy rate was more frequent in multiparous women(0,22%) than in nulliparous women(0,02%). The most common indication of cesarean hysterectomy was 13 cases of uterine atony(46%), followed by 5 cases uterine rupture(18%), 5 cases of placenta previa with placenta accreta(18%), 3 cases of placenta previa(11%), 2 cases of uterine myoma(7%). No significant difference in length of operating time, amount of blood loss and operative complications were found between total abdominal hysterectomy and subtotal hysterectomy. The all patient who had cesarean hysterectomy recieved from 1200ml to 15840ml of blood transfusion with a mean of 3673ml. In aspect of fetal outcome, 3 cases of FDIU(Fetal death in utero) in uterine rupture and 1 case of stillbirth in preterm were found. The maternal complications were wound disruption, DIC, wound hematoma, ureter injury, vaginal stump bleeding, UTI(Urinary tract infection) and retroperitoneal bleeding, Conclusion: We conclude that the incidence of emergency cesarean hysterectomy declined with prediction of antenatal risk factor, preparing of sufficient fresh blood, prophylactic antibiotics, vigorous uterotonics and close observation after delivery.

SSR 마커를 이용한 복숭아 품종의 유전적 다양성 분석

홍지화(Jee-Hwa Hong),이승인(Seung-In Yi),권용삼9Yong-Sham Kwon),김영(Young Kim),최근진(Keun-Jin Choi) 한국육종학회 2013 한국육종학회지 Vol.45 No.3

The objective of this study was to evaluate genetic diversity in 72 major peach varieties by using SSR markers. A set of 189 SSR primer pairs was screened and 74 primer pairs showed polymorphism in 9 varieties. Twenty primer pairs out of 74 primer pairs showed clear band pattern and repetitive reproducibility. The relationship between 20 markers genotypes and 72 varieties was analyzed. A total of 71 polymorphic amplified fragments were obtained by using 20 SSR markers. Two to nine SSR alleles were detected for each locus with an average of 3.6 alleles per locus. Average polymorphism information content(PIC) was 0.523, ranging from 0.246 to 0.771. A total of 71 marker loci were used to calculate Jaccard’s distance coefficients for cluster analysis using UPGMA. Clustering group was largely divided 2 groups according to absence or presence of pubescence on the fruit surface and genetic distance of cluster ranging from 0.39 to 1.00. Analysis of genetic diversity revealed that these 20 SSR marker sets discriminated a total of 68 varieties except for 4 mutant varieties among 72 varieties. These SSR markers will be utilized as molecular evidence in variety identification of peach.

국내 상추 육종 및 품종보호 출원경향

홍지화(Jee Hwa Hong),이호선(Ho Sun Lee),정희경(Hui Gyeong Jeong),황석중(Suk Jung Hwang) 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2