신뢰성 기준을 적용한 용수공급능력의 해석 : 낙동강유역을 중심으로 for the Nakdong River Basin

차상화,지홍기,이순탁 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

In general, the evaluation of water supply capacity is important factor to establish various establishment of water resource supply plan include water resource security and determination of dam's mass. But former researchs about estimation of water supply capacity were lack in continunity of evaluation basis, and didn't excute analysis on reliability criteria also. In this study, Nakdong river was selected for study basin, and then water supply capacity was analyzed by HEC-5 model using indentical reliability criteria.

주파수 도약 대역 확산 통신 방식의 동기 시스템 구현에 관한 연구

方孝昌,權智洪,張太和,金元厚 한국항공대학교 1992 論文集 Vol.30 No.-

주파수 도약 대역 확산 통신 방식에서 기존의 동기 시스템은 VCO의 비선형 특성, 루프의 온도보상문제, 소형화. 경량화 문제 등으로 시스템 구현에 많은 어려움을 겪고 있었다. 본 연구에서는 정합필터(matched filter)를 이용한 초기동기와 ADPLL(All Digital Phase Locked Loop)을 이용한 동기 추적 방식의 동기 시스템을 구현하였고, 초기동기시간 약 600μS, 동기추적에 약46mS의 시간을 얻어 전체 동기 시스템은 약 65mS이내를 얻었다. 일반적으로 동기시간이 100mS 이내에 이루어지면 완전한 동기를 이룬것으로 보며, 따라서 구현된 동기 시스템이 매우 안정되고 정확한 동기를 이루고 있음을 알 수 있었다. In the frequency hopping spread spectrum communication, the conventional synchronization systems have many problems in their implementation. It is nonlinear characteristics of VCO, temperature compensation of loop and implementing compactly. In this paper, we implemented the synchronization system which consists of the ACQUISITION by matched filter and the TRACKING by ADPLL(All Digital PLL). In this synchronization system, acquisition time and tracking time were about 600㎲, 64ms respectively, so that total synchronization time could be within 65ms. Generally, as synchronization time is within 100ms we can regard it as complete synchronization. therefore, we could prove that the implemented synronization system has a stable and accurate synchronization.

A Common Major QTL for Anthracnose Resistance in Two Different BC₁F₂ Populations Raised from an Interspecific Cross of Capsicum annuum × C. baccatum

Jee Hwa Hong(홍지화),Jae Wahng Do(도재왕),Dong Cheol Yang(양동철),Jae Bok Yoon(윤재복),Hyo Guen Park(박효근) 한국원예학회 2005 한국원예학회 학술발표요지 Vol.2005 No.10

Development of Novel SSR Markers using NGS sequencing and Genetic Relationship Analysis in Blueberry (Vaccinium spp.)

Jee-Hwa Hong,Eun-Jo Shim,Moo-Kyoung Yoon,Eun-Hee Soh 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

Blueberry (Vaccinium spp.) is a member of the Ericaceae and eleven varieties have been registered at the Korea Seed & Variety Service for Plant Variety Protection (PVP). This study was to develop simple sequence repeat (SSR) markers next generation sequencing (NGS) analysis and to analysis genetic relationship of blueberry 31 varieties. Highbush blueberry ‘Camellia’ and rabbiteye blueberry ‘Alapaha’ varieties were used as sequencing materials. Out of total 987 SSR primers detected between ‘Camellia’ and ‘Alapaha’, 148 SSR primers were initially applied to select SSR markers for identification of blueberry varieties. Fourteen SSR markers showed polymorphism between 8 varieties. Seven SSR markers showed reproducibility and clear peak among 14 SSR markers. Genetic relationships of 31 blueberry varieties were analyzed and identified using 7 SSR markers. A total of 30 polymorphic SSR alleles were obtained and two to seven alleles were detected for each locus with an average of 4.3 alleles per locus. Average polymorphism information content was 0.556, ranging from 0.374 to 0.714. Genetic distance of clusters ranged from 0.38 to 0.93 by unweighted pair-group method with arithmetical average based on Jaccard’s distance coefficients. These newly developed SSR markers indicate usefulness for variety identification related to seed dispute and distinctness, uniformity and stability (DUS) test for blueberry.

Database Construction for Variety Characteristics of Lettuce (Lactuca sativa L.) using SSR Markers

Jee-Hwa Hong,Yong-Sham Kwon,Kyoung-In Seo,Moo-Kyoung Yoon 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Seed & Variety Service for Plant Variety Protection (PVP). We previously constructed DNA profile database for identifying 159 lettuce varieties using 60 simple sequence repeat (SSR) markers. In this study, we selected optimum markers from previously applied markers and new SSR markers for the standardization of DNA profile database of 65 commercial lettuce varieties containing 18 PVP varieties distributed in Korea. Twenty-eight SSR markers from 60 SSR markers were selected for characterization with 65 commercial lettuce varieties according to the reproducibility, polymorphism, band pattern of marker and easiness of scoring. Out of 156 SSR primers, we additionally selected 11 new SSR primer pairs showed polymorphisms between 8 varieties and repetitive reproducibility on capillary electrophoresis system. Totally 127 polymorphic amplified fragments were obtained using 39 SSR markers. Two to seven SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Average polymorphism information content was 0.517, ranging from 0.281 to 0.771. Genetic distance of clusters ranged from 0.29 to 0.96 by unweighted pair-group method with arithmetical average based on Jaccard’s distance coefficients. A total of 65 commercial lettuce varieties were discriminated by 39 SSR marker genotypes. These SSR profile database developed will be continually characterized for the standardization of DNA profiles for lettuce commercial varieties

Species-specific QTLs Associated with Anthracnose Resistance to Different Colletotrichum spp. in an Introgressed BC1F2 Population from Capsicum annuum×C. baccatum

Jee Hwa Hong,Jae Wahng Do,Hyo Guen Park,Jae Bok Yoon 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Microsatellite 마커를 이용한 사과 품종 간 유전적 유연관계 분석

홍지화(Jee-Hwa Hong),권용삼(Yong-Sham Kwon),최근진(Keun-Jin Choi) 한국생명과학회 2013 생명과학회지 Vol.23 No.6

본 연구는 microsatellite 마커를 이용하여 국립종자원 서부지원에 수집된 사과 42품종에 대한 품종 간 유전적 유연관계를 분석하였다. 사과 품종식별에 적합한 마커를 선정하기 위하여 8개 품종을 대상으로 총 305개의 마커를 분석하였다. 8개 품종 간에 다형성이 높고, 반복간 재현성이 있으며 밴드패턴이 선명한 26개의 마커를 최종 선발하여 42품종을 대상으로 분석하였을 때 총 165개의 대립유전자가 분석되었다. 대립유전자의 수의 분포는 2~12개를 나타내었으며, 마커당 평균 대립유전자의 수는 6.4개로 조사되었다. PIC 값은 0.461~0.849의 범위에 속하였으며 평균값은 0.665로 나타났다. 165개의 대립유전자를 Jaccard 방법에 의해 유사도를 산출하고 비가중 산술방식에 의해 집괴 분석한 결과 공시품종의 유전적 거리는 0.27~1.00의 범위를 나타내었고, 총 42품종 중 41품종은 microsatellite 마커의 유전자형에 의해 구분되었다. 본 연구결과는 사과 품종의 식별을 위한 분자생물학적 자료로 유용하게 활용될 것으로 사료된다. The objective of this study was to evaluate the suitability of microsatellite markers for variety identification in 42 apple varieties. For microsatellite analysis, 305 primer pairs were screened in 8 varieties and twenty six primer pairs showed polymorphism with clear band pattern and repetitive reproducibility. A total of 165 polymorphic amplified fragments were obtained in 42 varieties using 26 markers. Two to twelve alleles were detected for each locus with an average of 6.4 alleles per locus. A value of polymorphism information content (PIC) ranged from 0.461 to 0.849 with an average of 0.665. A total of 165 marker loci were used to calculate Jaccard’s distance coefficients using unweighted pair-group method with arithmetical average (UPGMA) cluster analysis. Genetic distance of cluster ranged from 0.27 to 1.00. Analysis of genetic relationship revealed that these 26 microsatellite marker sets discriminated a total of 41 varieties except for 1 variety among 42 varieties. These markers will be utilized as molecular data in variety identification of apple.

전자눈을 이용한 햅쌀, 묵은쌀 및 이의 혼합쌀 판별 분석

홍지화(Jee-Hwa Hong),이재훤(Jae-Hwon Lee),조영호(Young-Ho Cho),최경후(Kyung-Hu Choi),이민휘(Min-Hui Lee),박영준(Young-Jun Park, and),김현태(Hyun-Tae Kim) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.5

본 연구는 햅쌀과 묵은쌀 및 이의 혼합곡 판별을 위하여 전자눈 분석을 이용한 쌀 신곡과 구곡 판별법 개발 연구를 수행하였다. 국내에서 수집된 신구곡을 대상으로 GOP 시약처리를 통해 효소 활성에 따른 정색 반응을 확인한 후 전자눈 장비를 이용하여 신곡과 구곡의 판별에 적합한 색깔 코드의 선별과 이를 이용한 쌀 신곡과 구곡의 판별법을 개발하였다. 미지시료를 이용하여 판별 정확도를 분석한 결과 신곡과 구곡인 단일곡은 100%의 정확도로 판별이 되었으나 혼합곡의 경우 혼합된 비율에 따라 판별 정확도가 달라졌다. 혼합곡은 신곡과 구곡의 혼합 비율에서 구곡이 비율이 높아질수록 판별 정확도가 높아지는 것으로 나타났다. 이러한 결과를 통해 전자눈 분석을 통하여 햅쌀과 묵은쌀을 판별할 수 있는 실용적인 판별 체계를 구축하였으므로 본 연구를 통해 개발된 판별식은 쌀 신구곡 판별을 위한 과학적인 근거자료로서 활용이 가능할 것으로 판단된다. The objective of this study was to develop methods for the discrimination of new and stale rice by using an electronic eye. To develop the discriminant, 107 rice samples produced in the years 2015 and 2016 were investigated. After the rice was treated with guaiacol, oxydol, and p-phenylenediamine reagents, an electronic eye was applied to discriminate between newly harvested rice and rice stored for 1 year. Out of the 4,096 color codes of the electronic eye, 31 color codes were identified for the discrimination of newly harvested rice and rice stored for 1 year. The classification ratio of newly harvested rice and rice stored for 1 year was 100% and the discrimination accuracy for unknown samples was 100%. In a total of 150 mixtures of new rice and stale rice, the discrimination accuracy was between 16.7 and 95.6%, depending on the mixing ratio. This capability of the electronic eye will be useful as a tool for discriminating the production year of rice.

SSR Marker를 이용한 감귤속 품종 및 유전자원에 대한 DNA Profile Data Base 구축

홍지화(Jee-Hwa Hong),채치원(Chi-Won Chae),최근진(Keun-Jin Choi),권용삼(Yong-Sham Kwon) 한국원예학회 2016 원예과학기술지 Vol.34 No.1

국내외에서 재배되고 있는 감귤속 식물 108 품종 및 유전자원과 SSR 마커를 활용하여 유전적 유사도 분석을 통한 품종식별력 검정 등에 대한 연구를 수행하였다. 감귤 8품종을 203개의 SSR 마커로 검정하여 반복 재현성이 높은 뿐만 아니라 다형성 정도가 높은 18개를 선정하였다. 이들 마커와 국내외에서 재배되고 있는 감귤 108품종을 검정하였을 때 마커당 평균 대립유전자수는 9.28개로 나타났고, 5-14개까지 다양한 분포를 나타내었다. PIC 값은 분자표지에 따라 0.417-0.791 범위에 속하였으며 평균값은 0.606으로 나타났다. 감귤류 108품종에 대하여 계통도를 작성하였을 때 감귤류 식물의 분류학적 특성 및 품종 육성의 계보도에 따라 13개의 그룹으로 크게 나누어졌다. 감귤류 식물 품종중 오렌지나 온주 밀감의 경우 대부분의 품종이 SSR 마커의 유전자형에 의해 구분이 되지 않은 것으로 나타났다 . 본 연구에서 개발된 감귤속 식물의 품종별 SSR DNA 프로파일 데이터베이스는 감귤속 식물의 유전자원 특성평가와 육종가의 지식재산권 보호의 수단으로 유용하게 활용될 수 있을 것이다. The present study investigated identification of cultivars through phylogenetic analysis of 108 Citrus varieties and related cultivars using simple sequence repeat (SSR) markers. Two hundred three SSR primer pairs were used to detect polymorphic markers among 8 Citrus cultivars consisting of 4 mandarins, 1 orange, 1 tangor, 1 tangelo, and 1 pumelo. Eighteen SSR primer pairs were reproducible and showed highly polymorphic alleles. These markers were applied to assess genetic variations of the 108 varieties. Each marker detected 5-14 alleles, with an average of 9.28. The polymorphism information content varied from 0.417 to 0.791 with an average of 0.706. Cluster analysis with SSR markers resulted in 13 major groups reflecting cultivar types and pedigree information. Twelve orange cultivars in the I-1st sub-cluster and 23 mandarin cultivars in the II-1st sub-cluster, respectively, were not discriminated using the SSR markers. This could be due to narrow genetic backgrounds originated through bud mutation or nucellars seedlings. The SSR profile database of Citrus cultivars will be useful as a tool for protection of plant breeders’ intellectual property rights in addition to assessing genetic diversity in Citrus cultivars and germplasms.

국내 상추 육종 및 품종보호 출원경향

홍지화(Jee Hwa Hong),이호선(Ho Sun Lee),정희경(Hui Gyeong Jeong),황석중(Suk Jung Hwang) 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2