Cd 處理에 對한 박달나무의 家系間 生理的 被害 및 抗酸化 反應 差異

吳昌泳,李景俊,李在千,韓心熙 한국임학회 2004 한국산림과학회지 Vol.93 No.7

본 연구는 Cd 처리에 대한 박달나무 유묘의 가계 간 엽록소 함량과 효소홀성에 의한 피해 및 항산화 내성 반응의 특성을 구명하고자 실시하였다. Cd 처리는 3가계의 박달나무 1년생 묘목에 0, 04, 08mM CdSO₄·8/3H₂O 용액을 이용하여 3수준으로 2개월간 실시하였다. 박달나무의 가계간 및 처리 간 피해내성반응은 엽록소 함량과 MDA(malondialdehyde) 함량, 단백질 함량, SOD(supetoxide dismutase) 활성을 이용하여 결정하였다. Cd 처리는 박달나무의 잎 내 광색소의 함량비를 변화시켰으며, 생리적 피해 지표인 MDA 함량은 모든 가계의 Cd 처리구에서 대소구보다 높게 나타났다. 또한 모든 Cd 처리구의 단백질 함량은 대조구보다 높았으며, MDA 함량과 단백질 한량은 Cd 처리 농도가 증가함에 따라 증가하였다. 한편 Cd 처리된 박달나무의 잎 내 SOD 활성은 대조구보다 증가하였으나, 0.8mM Cd 처리된 9번 가계 경우는 대조구보다 낮아졌다. 즉 박달나무는 Cd 처리에 의해 광색소 함량의 변화 및 MDA 함량 증가와 같은 생리적인 피해를 나타냄과 동시에 단백질 함량과 SOD 활성 증가를 통해 내성 반응을 나타냈다. 그러나 이리한 생리적인 피해 및 내성 반응은 가계 간에 뚜렷하게 달랐다. We investigated differences among half-sib families of Betula schmidtii seedlings in the effect of three Cd levels on physiological injury and antioxidative reaction. One-year-old seedlings of B. schmidtii were treated with 0, 0.4 and 0.8mM CCdSO₄·8/3H₂O for two months. Physiological injury and antioxidative reaction to three levels of Cd treatment determined with using photosynthetic pigments, MDA, protein contents, and SOD activity in the leaves of three half-sib families of B. schmidtii. The ratios of contents among photosynthetic pigments in the leaves of B. schmidtii were changed by Cd treatment. In additon, MDA content in the leaves of plants under Cd stress increased relative to control plants, and total protein content was also higher in the leaves of 0.4mM and 0.8mM Cd levels than control plants. The contents of MDA and total protein also increased with increasing the concentrations of Cd treatment. SOD activity in the leaves of three half-sib families increased by Cd treatment, but SOD activity of No. 9 family decreased relative to control plants at 0.8mM Cd level. In our trial, B. schmidtii treated with Cd represented physiological injuries such as the changes of photosynthetic pigments ratio and the increase of MDA content. At the same time, B. schmidtii showed tolerance responses against Cd toxicity through increasing the total protein content and SOD activity. But the physiological injury and tolerance responses were significantly different among three half-sib families exposed to 0.4mM and 0.8mM Cd.

六味知黃湯 投與가 高壓環境에 露出된 흰쥐의 血液造成과 臟器造織에 미치는 影響

오재근,강명신,조준용,이영일,최용어 韓國體育大學校附屬 體育科學硏究所 1996 韓國體育大學校附屬 體育科學硏究所論文集 Vol.15 No.1

This study was purposed to investigate the effect of yukmijihwangtang on rat blood components and organ histopathologic changes in the different environments of hyperbaric ambient air. The pharmacological effects of yukmijiwhangtang in oriental medicine have been reported to improve general condition and treat mental and physical weakness due to overload. 84 Male Sparague-Dawely rats, 200-250 g weight, were housed two per cage, fed Standard Rodent Diet with water ad libitum. The animals were assigned to 3 main groups for the blood components and gas analysis : normobaric control group (NC ; N=4), hyperbaric control group (HC ; N=8), hyperbaric medication group (HM ; N=8). HC and HM group were devided into 2 subgroups and kept on different situation of the hyperbaric ambient air (2ATA or 3ATA) for 2 hrs. HM group was administered 10cc extract of boiled yukmijiwhangtang before exposure. For histopathological analysis, hyperbaric groups(HC and HM : N=80) was further devided into 2 subgroups according to atmosphere absolute and exposure period : 2ATA (acute, 1, 2, 3, 4 wks), 3ATA (acute, 1, 2, 3, 4 wks). There were 20 groups in total, with 4 animals in each group. Experimental rats of HM group were administered 10cc extract of boiled yukmijiwhangtang 2 times per day and kept on situation of the hyperbaric ambient air (2ATA and 3ATA) 2 hrs per day for four weeks. In order to record the possible effects of yukmijiwhangtang in hyperbaric condition on the blood components, the rats were killed by decapitation directly after the hyperbaric exposure for 2 hrs. But the administered rats were killed on the morning following the last hyperbaric exposure during 4 wks. The samples were taken 2 hrs, 1, 2, 3, 4 wks after the begining of this study. Blood was collected from posterior abdominal vena cava immediately after decapitation. Organs for histopathological assays in hyperbaric groups (HC and HM) were also removed 2 hrs, 1, 2, 3, 4 wks and fixed at 10% formalin solution. The whole blood of 1㎖ was used for the analysis of pH, PO₂, PCO₂, HCO₃, BE, O₂CT, O₂SAT level by Blood Gas Autoanalyzer (Coning 175, U.S.A). And The rest whole blood of 1㎖ was utilized for the measurement of WBC (white blood cell), RBC (red blood cell), Hb (hemoglobin), Hct (hematocrit), MCV (mean corpuscular volume), MCH (mean corpuscular hemoglobin) level by Microcell Counter (Model; CC-170-TDA Co., Japan). Hematological and blood gas analysis and organ histopathological changes after exposure to hyperbaric ambient air were as follow; (1) Hematological parameters were lower in the hyperbaric medication group than all the control groups without WBC, MCV (2ATA) and MCV, MCH (3ATA). But a statistical significance among the groups was not shown. (2) The hyperbaric medication group was lower than another control groups on all parameters of blood gas analysis after 2 hours exposure to hyperbaric ambient air (2ATA and 3ATA) without O₂SAT. But a statistical significance among the groups was not shown. (3) On observing the histopathological system of the lung with microscope, the congestion, edema and hemorrhage on the alveolar sacs and alveolar wall damage in the hyperbaric control group were heavier than in the hyperbaric medication group.

Ten-Two mechanics에 의한 고정원 준비시 하악구치와 전치 주위조직의 응력 분포에 관한 광탄성법적 분석

오준기,성재현 慶北大學校 齒科大學 1992 慶北齒大論文集 Vol.9 No.1

하악의 고정원 형성시 Ten-Two mechanics을 사용할때 하악구치와 하악전치의 치근 주위조직에 미치는 영향을 평가하기 위하여, 제1소구치 발치 증례에서 평준화와 전치부의 견인이 끝난 에폭시모형을 제작하였다. 에폭시모형상상에서 Ten-Two mechanics와 high pull J-hook headgear를 사용하여 준비고정한 것과 Ⅲ급 고무로 준비고정 하였을 때, 하악구치와 전치 주위조직에 나타난 응력 분포 상태를 광탄성법으로 비교 분석하여 다음과 같은 결과를 얻었다. · Ⅲ급 고무를 사용시, 하악 구치부에 후방경사이동 양상이 전반적으로 나타났으며, 전치부에는 압입의 양상이 상당히 나타났다. · 10-2-7 mechanics와 high pull J-hook headger를 사용시, 하악 제2대구치에 후방경사이동 양상이 나타났고, 전치부에는 거의 압입의 양상이 나타나지 않았다. · 10-2-6 mechanics와 high pull J-hook headger를 사용시, 하악 제1대구치에 후방경사이동 양상이 나타났고, 전치부에는 거의 압입의 양상이 나타나지 않았다. · 10-2-5 mechanics와 high pull J-hook headger를 사용시, 하악 제2소구치에 후방경사이동 양상이 나타났고, 전치부에는 거의 압입의 양상이 나타나지 않았다. This study was performed to analyze the effects of Ten-Two mechanics applied to prepare mandibular anchorage on the surrounding tissues of the mandibular molars and incisors. The photoelastic model was constructed as the premolar extraction case which leveling and retraction of anteriors were finished. In this epoxy resin model, mandibular anchorage was prepared by two methods, that was Ten-Two mechanics with high pull J-hook headgear and Class Ⅲ elastics. The obtained stress distribution on the surrounding tissues of the roots was analyzed by photoealstic method. The results were as follows: 1. When Class Ⅲ elastics were used in preparing mandibular anchorage, distal tipping movement tendency occurred overally in the madibular molars and considerable intrusion occurred in the incisors. 2. When 10-2-7 mechanics with high pull J-hook headgear used in preparing mandibular anchorage, distal tipping movement tendency occurred in the mandibular second molar and the least intrusion occurred in the incisors. 3. When 10-2-6 mechanics with high pull J-hook headgear used in preparing mandibular anchorage, distal tipping movement tendency occurred in the mandibular first molar and the least intrusion occurred in the incisors. 4. When 10-2-5 mechanics with high pull J-hook headgear used in preparing mandibular anchorage, distal tipping movement tendency occurred in the mandibular second premolar and the least intrusion occurred in the incisors.

4주간의 육미지황탕 투여가 최대하운동시 근대 5종 선수들의 혈액성분 및 혈액가스성분 변화에 미치는 영향

오재근,최용어,서인원,조준용,유루리 韓國體育大學校 1997 論文集 Vol.20 No.-

The purpose of this study was to estimate the changes of blood components and blood gases on submaximal exercise after Yuk-mi-ji-Whang-tang administration during 4 weeks. Ten modern pentathlon athletes participated as subjects of this study. The result and conclusion of this study is as follows; The changes of blood components on pre-, post-sumaximal exercise and during recovery time was not significant difference in Yuk-mi-ji-whang-tang administration group except for WBC(p<.05.). But comparative observation of blood gases levels such as pH, PCO₂, PO₂, HCO₃, O₂SAT, O₂CT was showed a tendency of being lower acidity, higher PO₂, lower PCO₂on pre-, post-sumaximal exercise and during recovery time in Hyang-sa-pyung-yi-san than in Yuk-mi-ji-whang-tang administration group.

농촌지역 초등학교 유휴시설의 활용방안에 관한 연구 : 해남군 초등학교를 중심으로 Focused on the Elementary School in Hae-Nam

오재섭,이봉수,박향용,유창균,조용준 朝鮮大學校 建設技術硏究所 1998 建設技術硏究 Vol.18 No.2

Recently, number of elementary school students in the rural area have decreased due to move of population to the diminishment of birth population, the generation of idle facilities and even the trend of abolition of school presented problems. This paper was a study of the use plans and generation pattern of idle facilities in present school. And the targeted schools of this study were elementary schools located at Hae-Nam Province in Chun-Nam. The results are as follows : 1) While the number of the elementary school is continuosuly decreased up to now since 1980, the number of branch schools and closed schools is increasing and while general class is decreased continuously, multiple classes are increasing and so the utilization of idle facilities will be continued. 2) The utilization of idle facilities is most frequent in the use of school curriculm(67%), followed by the use of club activities and welfare facilities, but the use by residents is minimum. In the problems in using idle facilities, the lack of finance is most(68%), followed by the lack of illustrative data and technical problems and in the effects of using idle facilities, extra curriculum activities are best and followed by the use of local community and school curriculum. 3) In the most required facilities in using idle facilities, gymnasium and service area are highest and followed by laboratory, learning data room and library and in the common facilities with local residents, swimming pool is most frequent and followed by library.

광합성세균 미생물막반응기에 의한 유기성폐수의 처리특성

오광근,이철우,전영중,이재홍 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

홍색비유황 광합성세균인 Rhodopseudomonas capsulata를 선택하여 porous ceramic bead를 충진시킨 충진형 반응기(Packed-bed reactor, PBR)와 분말활성탄을 현탁시킨 유동산 반응기(Fluidized-bed reactor, FBR)에서 미생물막을 형성하여 폐수처리를 비교한 결과 PBR이 FBR보다 BOD 부하량 변화에 더 안정적인 처리성을 보였다. 체류시간 (hydraulic retention time, HRT)에 따른 처리성은 유입폐수 농도를 각각 BOD 10,000, 20,000 mg/ℓ로 하였을 때, HRT 1일 이상에서 90% 이상의 처리효율을 가지며, 유출수의 BOD 농도는 각각 350, 800 mg/ℓ이었다. PBR에서 유입수의 BOD 농도변화에 다른 유출수의 농도를 측정하여 단위부피당 기질부하량 상수 (maximum specific BOD loading rate, P) 및 부착미생물 포화기질 제한농도 상수(half saturation constant, K_6)를 구한 결과, 각각 22.2 gBOD/ℓ·day, 1,750 mgBOD/ℓ이었고, 처리효율 90% 이상을 나타내는 BOD 용적부하(volumetric BOD loading rate)는 20 gBOD/ℓ·day 이상으로 표준활성오니법의 0.6 gBOD/ℓ·day에 비하여 30배가 넘는 값을 나타내었다. An efficient packed-bed type biofilm reactor charged with immobilized phototrophs was developed to treat organic wastewater at an extremely high volumetric loading rate. The packed bed reactor (PBR) charged with porous ceramic beads was superior to a fluidized-bed reactor suspended with activated carbon powders in terms of many aspects such as BOD removal efficiency, operational stability, and overall economics. For wastewater with BOD concentration as high as 20,000 mg/l, the BOD removal efficiency was maintained above 90% when the hydraulic retention time (HRT) was longer than 1 day. The allowable volumetric BOD loading rate of this reactor (20gBOD/l·day) is more than ten-folds higher than that of an ordinary activated sludge method. The behaviour of the reactor was represented well by a Monod type kinetic equation with a maximum specific BOD loading rate(P) of 22.2gBOD/l·day and a half saturation constant(K_s) of 1,750 mgBOD/l.

경남지역 건강검진자에서의 혈청지질에 관한 연구

정준훈,박현모,류재오,조동자,표미순,오은경 한국건강관리협회 2000 건협 Report Vol.3 No.-

자동판매기 이용자행동에 관한 연구

김재륜,홍영준,최재오 대구산업정보대학 2001 논문집 Vol.15 No.-

This study guesses the actual used condition of a vending machine for consumers and offers the marketing suggestion to a vending machine management according to researching difference to a primary factor using a vending machine as the population statistics quality of a vending machine users. This research of questions investigates a experienced person living in Tae-gu or Kyung-buk and using a vending machine once at least. Researching effect of a vending machine use as the population statistics quality. The problem 1 of study is accepted. That is to say, it turns up the difference to a primary factor using a vending machine as age. The problem 2 of study about each different property using a vending machine as the distinction of sex is rejected. it suggests that a marketing strategy plan as the distinction of sex should be unimportant. The problem 3 of study about each different property using a vending machine as an occupation is accepted. The offices show that they think the important points using a vending machine which are conditions of clean lines, rest facilities of the circumference, safety and trust etc.

PVA 복합체의 표면 성분과 구조 : PVA/Gelatin/Borax film PVA/Gelatin/Borax 필름

김재문,오준,김명렬,O'Donell, James H.,Jill, David J.,Pomery, P.J. 順天大學校 1999 論文集 Vol.18 No.1

Surface composition and structure of PVA complexes (PVA/Gelatin/Borax) films were investigated with XPS and the bulk content was measured with ¹H-Neuclear Magnetic Resornance spectrometry (¹H-NMR). From this results, it is possible to calculate of carbon, oxgen, nitrogen, etc and showed hydroxyl and aminogroups. Crystallinities of PVA was measured by infrared and X-ray diffractometer. On the basis of the experimental results, the structure to the PVA complexes in various materials were estimated. PVA complexes have a uniform structure with flexible PVA chains in a mixed solvent of water.

가성부갑상선 기능저하증 환아의 구강 증상 : CASE REPORT

김성오,홍은경,최형준,이제호,손흥규 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

부갑상선 호르몬은 혈중 인농도를 낮추고, 칼슘농도를 증가시키는 호르몬으로 부갑상선기능저하증은 부갑상선이 존재하지 않거나 비정상적으로 기능하여 이 호르몬의 분비가 감소되는 질환이며, 가성부갑상선기능저하증은 부갑상선 호르몬 분비는 정상이나, 이 호르몬에 대한 괄과 신장의 반응이상으로 혈중 칼슘 농도가 감소되고 인농도가 증가되는 질환이다. 가성부갑상선기능저하증은 임상적으로 성장 및 발육부전, 둥근 얼굴, 조기 골단폐쇄로 인한 단지증, 이소성 연조직 석회화, 비만, 두개관의 비후, 정신지체, 백내장 등이 나타난다. 구강내 소견으로는 치아의 맹출지연, 법랑질형성부전이 가장 큰 특징이며, 그 외에 결손지, 부정교합, 높은 구개궁, 확장된 치근관과 짧은 치근, 치수강 내 석회화, 높은 우식 이환율, 치근막의 비후 등이 관찰된다. 본 증례에서 환아의 구강내 소견은 맹출한 하악 4전치와 상악 좌우측 영구 중절치, 상악 우측 영구 측절치, 상, 하악 제 1대 구치에서 법랑질형성부전을 보였고, 다수의 유치에서 심한 동요도를 보였다. 방사선 소견으로는 대부분의 미맹출된 영구치들도 법랑질형성부전이 관찰되었으며, 특히 영구 견치의 경우 심한 치관의 형태이상을 볼 수 있었다. 또한 유치의 비정상적인 치근흡수가 나타났고, 하악 전치의 경우 치근형태가 짧고, 치근단이 둥근 형태로 나타났다. 이에 저자는 가성부갑상선기능저하증인 환아의 구강내 소견에 대해 보고하고자 한다. The parathyroid hormone plays a major role in the metabolism of calcium and phosphorus. In hypoparathyroidism the parathyroid glands are atrophied or absent associated with autoantibodies against parathyroid tissue. Pseudohypoparathyroidism is a metabolic disease caused by the disturbance in peripheral action of parathormone, but parathormone level is normal. In general, patients with pseudohypoparathyroidism have short stature, round face, brachydactylia, obesity, mental retardation, cataracts & ectopic calcifications on soft tissues. Dental manifestations are enamel hypoplasia, delayed eruption, blunting of root apex, hypodontia, pulp calcification, thickened lamina dura, excessive caries & malocclusion. In this case, intraoral examination showed enamel hypoplasia on the erupted permanent teeth & hypermobility on the remaining deciduous teeth. From the radiographic view, severe dental anomalies were observed on canines and shortening and blunting of root apex was observed on mandibular incisors. Pathologic root resorption was also observed on deciduous teeth.