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( Seung Han Kim ),( Hoon Jai Chun ),( In Kyung Yoo ),( Jae Min Lee ),( Seung Joo Nam ),( Hyuk Soon Choi ),( Eun Sun Kim ),( Bora Keum ),( Yeon Seok Seo ),( Yoon Tae Jeen ),( Hong Sik Lee ),( Chang Duc 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Copper ions are trace elements which act as cofactors of superoxide dismutase with antioxidant effect and is related to reduce molecular oxygen in living system. Several studies have demonstrated that serum copper ion level are elevated in several malignancies. But, there was no multiphoton(MP) probe which can measure quantifi ed value of copper ions in intact tissue without grinding or drying off. The study aim was to develop ratiometric MP probe for measuring quantified value of Cu2+ and observe colon neoplasm with MP microscopy. Methods: We have developed a two-photon(TP) probe for Cu2+ with an internal reference. We have employed an extended coumarin as the fl uorophore (FL, red emission) having 2-picolymethylamide moiety at the 2-position as the Cu2+ chelator. FL was connected through a piperazine linker to a chromene derivative, which was employed as an internal reference (IR, blue emission). We had validated the two-photon excited fl uorescence (TPEF) intensity ratio of FL and IR so that a quantitative measurement of Cu2+ in live tissue was possible. We performed current study with validated ratiometry probe. Tissues of normal colon mucosa, colon adenoma and colon cancer obtained during colonoscopic biopsy were stained with MP probes for Cu2+ and were observed with MP ratiometry probe. Results: We could measure a quantitative measurement of Cu2+ in fresh intact colon mucosal tissue with ratiometric multiphoton probe. The concentration of Cu2+ of colonic adenocarcinoma was 16.9uM (range 15-18), adenoma 13.5 uM (range12.5-14.5), normal colon mucosa 11.5 uM (range 11-12.1), respectively. Conclusions: Cu2+ quantifi cation was possible with multiphoton ratiometry probe in fresh intact colon neoplasm tissues. Future study is warranted to assess antioxidant status of neoplasm tissues using probe based multiphoton microscopic analysis.
Rengaraj, Deivendran,Truong, Anh Duc,Hong, Yeojin,Pitargue, Franco Martinez,Kim, Jong Hyuk,Hong, Yeong Ho,Han, Jae Yong,Kil, Dong Yong Elsevier 2019 Research in veterinary science Vol.123 No.-
<P><B>Abstract</B></P> <P>Among the eight forms of vitamin E, the liver preferentially releases α-tocopherol into the circulation and it is distributed to the non-liver tissues. In the hepatocytes, alpha tocopherol transfer protein (TTPA) specifically recognizes α-tocopherol with 2<I>R</I>-configuration and facilitates its intracellular transfer. The identification and characterization of TTPA expression have not been demonstrated in avian species. Therefore, the objectives of this study were to identify avian TTPAs, to compare the sequence conservation, phylogenetic relationship, protein interactions, and disease associations of chicken TTPA with those of human and vertebrate TTPA, and to characterize the tissue expression of the TTPA gene in chickens fed diets supplemented with different amounts of α-tocopherol. Our results suggest that the chicken TTPA was highly conserved with the human and vertebrate TTPA, and consisted of a cellular retinaldehyde binding protein and TRIO guanine exchange factor (CRAL_TRIO) domain. Feeding diets supplemented with increasing amounts of α-tocopherol (25 IU/Kg, 50 IU/Kg, or 100 IU/Kg) to broiler chickens had no effects on growth performance compared with feeding basal diets containing no supplemental α-tocopherol. The expression of TTPA gene was detected high in the liver of chickens in response to dietary α-tocopherol concentrations, whereas its expression was very low or undetectable in the non-liver tissues. In conclusion, the chicken TTPA protein sequence is highly conserved with other avian and vertebrate TTPA protein sequences. The higher expression of TTPA gene in the chicken liver in response to dietary α-tocopherol concentrations may suggest its crucial role in transporting α-tocopherol in the chicken liver.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 21-day chickens were fed diets with 0–100 IU DL-α-tocopheryl acetate for 14-days. </LI> <LI> Dietary α-tocopherol levels had no effects on growth performance of chickens. </LI> <LI> TTPA gene expression was high in the liver of chickens fed with α-tocopherol. </LI> <LI> Higher level of TTPA in the chicken liver suggest its role in α-tocopherol transfer. </LI> </UL> </P>
Paik, Man-Jeong,Nguyen, Duc-Toan,Yoon, Jae-Hwan,Chae, Han-Seung,Kim, Kyoung-Rae,Lee, Gwang,Lee, Pyung-Cheon Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.7
The enantiomeric separation of lactic acid for its absolute configuration has become important task for understanding its biological origin and metabolic process involved in the formation of lactic acid. It involves the conversion of enantiomers as diastereomeric O-pentafluoropropionylated (S)-(+)-3-methyl-2-butyl ester and the direct separation by gas chromatography-mass spectrometry on a achiral capillary column. The (R)- and (S)-lactic acids were completely separated with a high resolution of 1.9. The newly developed method showed good linearity (r ${\geq}$ 0.999), precision (% relative standard deviation = 3.4-6.2), and accuracy (% relative error = -7.7-1.4) with the detection limit of 0.011 ${\mu}g/mL$. When the method was applied to determine the absolute configuration of lactic acid in Lactobacillus delbrueckii subsp. lactis 304 (LAB 304), the composition (%) of (R)-lactic acid in the cell pellet and in the culture medium were $89.0{\pm}0.1$ and $78.2{\pm}0.4$, respectively. Thus, it was verified that the present method is useful for the identification and composition test of lactic enantiomers in microorganisms.
Rengaraj, Deivendran,Truong, Anh Duc,Lillehoj, Hyun S.,Han, Jae Yong,Hong, Yeong Ho Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.9
Objective: Defensins are a large family of antimicrobial peptides and components of the innate immune system that invoke an immediate immune response against harmful pathogens. Defensins are classified into alpha-, beta-, and theta-defensins. Avian species only possess beta-defensins (AvBDs), and approximately 14 AvBDs (AvBD1-AvBD14) have been identified in chickens to date. Although substantial information is available on the conservation and phylogenetics, limited information is available on the expression and regulation of AvBD8 in chicken immune tissues and cells. Methods: We examined AvBD8 protein expression in immune tissues of White Leghorn chickens (WL) by immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (RT-qPCR). In addition, we examined AvBD8 expression in chicken T-, B-, macrophage-, and fibroblast-cell lines and its regulation in these cells after lipopolysaccharide (LPS) treatment by immunocytochemistry and RT-qPCR. Results: Our results showed that chicken AvBD8 protein was strongly expressed in the WL intestine and in macrophages. AvBD8 gene expression was highly upregulated in macrophages treated with different LPS concentrations compared with that in T- and B-cell lines in a time-independent manner. Moreover, chicken AvBD8 strongly interacted with other AvBDs and with other antimicrobial peptides as determined by bioinformatics. Conclusion: Our study provides the expression and regulation of chicken AvBD8 protein in immune tissues and cells, which play crucial role in the innate immunity.
장복현,한재덕,김용대,차영욱,조경록,조태원 ( Bog Hyun Jang,Jae Duc Han,Yong Dai Kim,Young Uk Cha,Kyung Rof Cho,Tae Won Cho ) 충북대학교 산업과학기술연구소 1995 산업과학기술연구 논문집 Vol.9 No.1
Abstract_Roman We implemented a graphic user interface(GUI) which sends and receives data between two pe- rsonal computers through RS-232C. The transmitted data are displayed at the determined loca- tion on the GUI of another computer. There can be many