Quantifying herbicide dose-response and resistance in <i>Echinochloa</i> spp. by measuring root length in growth pouches

Zhang, C. J.,Lim, S. H.,Kim, J. W.,Song, J. S.,Yook, M. J.,Nah, G.,Valverde, B. E.,Kim, D. S. Canadian Science Publishing 2015 Canadian journal of plant science. Revue canadienn Vol.95 No.6

<P> Zhang, C. J., Lim, S. H., Kim, J. W., Song, J. S., Yook, M. J., Nah, G., Valverde, N. E. and Kim, D. S. 2015. Quantifying herbicide dose-response and resistance in Echinochloa spp. by measuring root length in growth pouches. Can. J. Plant Sci. 95: 1181-1192. The aim of the presented study was to develop a bioassay for rapid diagnosis of herbicide dose-response and resistance in Echinochloa. Pre-germinated seeds of Echinochloa spp. were incubated in growth pouches (18 cm×16.5 cm) containing herbicide solutions in a range of concentrations. Shoot and root lengths were measured after 6 d of incubation. Dose-responses estimated by measuring root lengths in the growth pouches were well-described by the log-logistic dose-response model and similar to those estimated by a whole-plant assay. Accurate dose-response curves were successfully generated for several herbicides with different modes of action, suggesting that the growth pouch method can be used for herbicide bioassays. The suitability of the growth pouch method for rapid diagnosis of acetyl coenzyme-A carboxylase (ACCase) and acetolactate synthase (ALS) inhibitor resistance in Echinochloa spp. was also tested. For cyhalofop-butyl, resistant and susceptible biotypes were discriminated at 180-300 mg a.i. L<SUP>−1</SUP> and 80-120 mg a.i. L<SUP>−1</SUP> for barnyardgrass (E. crus-galli) and late watergrass (E. oryzicola), respectively. For penoxsulam, the discriminatory dosage was 350-500 mg a.i. L<SUP>−1</SUP> for barnyardgrass and 650-1000 mg a.i. L<SUP>−1</SUP> for late watergrass. The method was further used to identify late watergrass biotypes resistant and susceptible to two other ALS inhibitors, azimsulfuron and bispyribac-sodium. Our results show that the growth pouch method can be reliably used in herbicide dose-response studies and to diagnose herbicide resistance in Echinochloa spp., with significant time and cost savings compared with conventional whole-plant assays. </P>

Glycerol-fed microbial fuel cell with a co-culture of Shewanella oneidensis MR-1 and Klebsiella pneumonae J2B

Kim, C.,Song, Y. E.,Lee, C. R.,Jeon, B. H.,Kim, J. R. SPRINGER 2016 Journal of industrial microbiology & biotechnology Vol.43 No.10

<P>Glycerol is an attractive feedstock for bioenergy and bioconversion processes but its use in microbial fuel cells (MFCs) for electrical energy recovery has not been investigated extensively. This study compared the glycerol uptake and electricity generation of a co-culture of Shewanella oneidensis MR-1 and Klebsiella pneumonia J2B in a MFC with that of a single species inoculated counterpart. Glycerol was metabolized successfully in the co-culture MFC (MFC-J&M) with simultaneous electricity production but it was not utilized in the MR-1 only MFC (MFC-M). A current density of 10 mA/m(2) was obtained while acidic byproducts (lactate and acetate) were consumed in the co-culture MFC, whereas they are accumulated in the J2B-only MFC (MFC-J). MR-1 was distributed mainly on the electrode in MFC-J&M, whereas most of the J2B was observed in the suspension in the MFC-J reactor, indicating that the co-culture of both strains provides an ecological driving force for glycerol utilization using the electrode as an electron acceptor. This suggests that a co-culture MFC can be applied to electrical energy recovery from glycerol, which was previously known as a refractory substrate in a bioelectrochemical system.</P>

전주 근교 농장의 자돈에서 면역형광항체법을 이용한 돼지콜레라 혈청 중화항체가 조사

허창열,김영진,노수일,김진구,송희종,김종면 全北大學校 附設 畜産開發硏究所 1991 畜産開發硏究報告 Vol.4 No.-

In order to evaluate the effect of vaccinantion for hog-cholera virus, sera were collected from each 20 piglet out of 26-32 days old before vaccination, and then from the 14th and 28th day post-vaccination(PV) with LOM live virus vaccine at the J and C swine ranches in the suburbs of Chonju. Monoclonal antibody(MAb) against ALD strain of hog cholera virus(HCV) and goal antimouse FITC-conjugates were employed in indirect immunofluorescence(IF) test for the titration of HCV and neuralization IF test for serum neutralization(SN)-Ab-titers against HCV. SN-Ab-titers(M±S.D.) were <2±0 in all piglets before vaccination at the both swine ranches. Whereas SN-Ab-titers of the J and C swine ranches were 111.2±115.08 and 144±121.7 at the 14th day PV and were 361.6±279.6 and 723.2±966.2 at the 28th day PV, respectively. Compared with Duncan's test, SN-Ab-titers were not significantl difference between the H and C swine ranches of the 14th day PV, but were significantly difference at the 28th day PV (P<0.0009). Therefore, SN-Ab-titers were significantly difference between the 14th and 28th day at the J or C ranches, respectively(P<0.0007, P<0.0114). Excepting SN-Ab-titer was1:16 in one piglet of the 14th day PV at the J swine ranch, defensible levels of SN-Ab-titers could be detected in all piglets PV of the both swine ranches. It is suggested that varied dffect of vaccination against HCV may be depended by feed and mangement, enviroment, sanitary conditions, breed and pedigree or vaccination program, etc.

Features and Properties of $YBa_2$$Cu_3$$O_{7-x}$ Films Grown on SrTi$O_3$ by High Frequency PLD

Shi, D.Q.,Ko, R.K.,Song, K.J.,Chung, J.K.,Choi, S.J.,Park, Y.M.,Shin, K.C.,Yoo, S.I.,Park, C. The Korean Superconductivity Society 2003 Progress in superconductivity Vol.5 No.1

YBCO films were deposited with various thicknesses from 100nm to 1.6$\mu\textrm{m}$ on single crystal $SrTiO_3$ substrates by pulsed laser deposition (PLD). The effects of different deposition conditions, especially different deposition rates by means of changing the pulsed laser frequency up to 200Hz, on the J$_{c}$ value were studied. For YBCO film with the thickness of 200nm, the $J_{c}$ value of $2.1MA/\textrm{cm}^2$ has been achieved under the high deposition rate of 3.2nm/s (190nm/min). The $J_{c}$ can be maintained greater than $1M/\textrm{cm}^2$ with the thickness less than 1$\mu\textrm{m}$. The X-ray analysis was used to examine the texture, crystallization and surface quality. The SEM was employed to analyze the surface of YBCO, and it was shown the surface of YBCO film became rougher with increasing the thickness. There were many large singular outgrowths and networks of outgrowths on the surface of YBCO films which lowered the density of thick YBCO film. The outgrowth network was probably the a-axis YBCO corresponding to XRD $\theta$-2$\theta$scan and $\chi$-scan which were used to characterize a-axis orientation of YBCO film. The reason for J$_{c}$ declining with increasing the thickness was studied and discussed.sed.

A STUDY on FOREST FIRE SPREADING ALGORITHM with CALCULATED WIND DISTRIBUTION

Song,J,H,Kim,E,S,Lim,H,J,Kim,H,Kim,H,S,Lee,S,Y 한국화재소방학회 1997 한국화재소방학회 학술대회 논문집 Vol.1997 No.-

There are many parameters in prediction of forest fire spread. The variables such as fuel moisture, fuel loading, wind velocity, wind direction, relative humidity, slope, and solar aspect have important effects on fire. Particularly, wind and slope factors are considered to be the most important parameters in propagation of forest fire. Generally, slope effect cause different wind distribution in mountain area. However, this effect is disregarded in complex geometry. In this paper, wind is estimated by applying computational fluid dynamics to the forest geometry. Wind velocity data is obtained by using CFD code with Newtonian model and slope is calculated with geometrical data. These data are applied fer 2-dimentional forest fire spreading algorithm with Korean ROS(Rate Of Spread). Finally, the comparison between the simulation and the real forest fire is made. The algorithm spread of forest fire will help fire fighter to get the basic data far fire suppression and the prediction to behavior of forest fire.

Microstructural Properties of the Insoluble Residue in a Simulated Spent Fuel

Kim, J. S 한국원자력학회 1998 Nuclear Engineering and Technology Vol.30 No.2

Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

Microsatellite 를 이용한 돼지 Yorkshire 품종의 일당중체량 연관 DNA Marker 개발

송영민,김철욱,조광근,박외선,권은정,이정규,백동훈,최진성,노정만,홍연희 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was conducted to develop DNA markers related with average daily gain (ADG) using 27 pigs. Animals were divided into two groups: low ADG group had 11 pigs with 1.5 SD and high ADG group was consisted of 16 pigs with +1.5 SD. Initially, we choose and tested 78 microsatellites which could contain potential DNA markers for ADG. Part of the microsatellites were amplified by the polymerase chain reaction (PCR) and then electrophoresed on a agarose gel followed by the allelic, genotypic and statistical analysis. According to the alleic frequency analysis, 21 DNA polymorphisms were detected from 17 microsatellites and appeared to be associated with ADG (P$lt;0.05). From the genotypic frequency analysis, 14 DNA polymorphisms were found from II microsatellites and were associated with ADG. All pigs in the low ADG group had a 135:144 hetero type for the SWR308 marker, whereas all pigs in the high ADG group had a 144:144 homo type. This and possibly other selected markers could be useful for the selecting young pigs with an excellent growth potential.

아밀로이드 펩타이드에 의한 세포독성에 있어서 미토콘드리아의 기능저하와 산화적 스트레스의 유발

송지현,박정순,최정윤,이영희,한복기 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Vertex Epidural Hematomas: Considerations in the MRI 1 ra

Song, J.-H Korean Academy of Medical Science 1996 Journal of Korean medical science Vol.11 No.3