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Shin, Hyunsuk,Oh, Youngjae,Kim, Daeil Blackwell Publishing Ltd 2015 Physiologia plantarum Vol.154 No.4
<P>To boost our understanding of a recent outbreak of freezing injury, we sought to confirm distinctive features between the shoot tissues of the peach (<I>Prunus persica</I>) cultivars Daewol and Kiraranokiwami by mimicking unseasonable changes of temperatures that occur in the early spring through repeated deacclimation and reacclimation treatments. Patterns of cold hardiness declined dramatically during the deacclimation and rose during the reacclimation in both cultivars. Our results indicated that ‘Daewol’ possessed higher capacity in response to repeated deacclimation and reacclimation treatments than ‘Kiraranokiwami’. ‘Daewol’ showed more sensitive changes in the carbohydrates in response to warm and low temperatures compared with ‘Kiraranokiwami’. ‘Daewol’ indicated almost similar repeated down‐ and up‐patterns in soluble sugar content in response to repeated deacclimation and reacclimation, whereas it indicated repeated up‐ and down‐patterns in starch content. However, ‘Kiraranokiwami’ showed a progressive increase in the soluble sugar content and a progressive decrease in starch content. Notably, patterns of accumulation of a 60‐kDa dehydrin protein encoded by the <I>PpDhn1</I> gene were confirmed through western blotting and paralleled fluctuations of cold hardiness in both cultivars. Expression of this dehydrin was weak in both cultivars during deacclimation but its band intensity increased during reacclimation. Changes in related genes (<I>β‐amylase</I>, <I>PpDhn1</I>, <I>PpDhn2</I> and <I>PpDhn3</I>) were positively correlated with changes in cold hardiness throughout the experiment. Our results indicate that recent repeated warm periods may cause premature deacclimation in the early spring, and that more cold‐tolerant cultivar may be more resilient to freezing injury caused by unstable temperature conditions.</P>
Hyunsuk Shin,Sujeong Park,Seongho Lee,Yeonju Park 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The study was investigated to compare growth characteristics of two Centella asiatica cultivars (‘Giant Tiger Care’ and ‘Good Tiger Care’), and their antioxidant activity and triterpenoid content. Plant size was greater in ‘Giant Tiger Care’ than in ‘Good Tiger Care’ at harvest time. Antioxidant activity and total phenol content in four extracts (WE, water extract; HWE, hot water extract; 50E, 50% EtOH extract; 70E, 70% EtOH extract) of two cultivars were high in 70E of ‘Giant Tiger Care’. As a result of triterpenoid content analysis, the major triterpenoids of the two cultivars were identified as madecassoside and asiaticoside. The total triterpenoid content was high in 50E and 70E of ‘Giant Tiger Care’ and ‘Good Tiger Care’, respectively but the total triterpenoid content was highest in 70E of ‘Good Tiger Care’. However, at the 104 days after planting, the yield of ‘Giant Tiger Care’ was three times higher than that of ‘Good Tiger Care’. In addition, the total triterpenoid content that can be produced in the same cultivation area (3.3 m²) was 2.459 mg in 50E of ‘Giant Tiger Care’, which was ∼2.2 times higher than that of 70E of ‘Good Tiger Care" (1.103 mg).
Hyunsuk Shin,Sewon Oh,Keumsun Kim,Youngjae Oh,Jungyeon Won,Hyeondae Han,Daeil Kim 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
Proline (Pro) accumulation is a common physiological reaction in response to abiotic stresses in many plants. Accumulation of Pro is believed to play the important role in protecting cellular components from dehydrating effects due to such stresses. The study was performed to investigate the relationship between cold hardiness and Pro content or expression of related genes in peach cultivars during a constant experimental deacclimation. Changes in cold hardiness were determined using electrolyte leakage method in the shoots of 10 peach cultivars (Prunus persica ‘Aikawanakajima’, ‘Chiyomaru’, ‘Daewol’, ‘Janghowon Hwangdo’, ‘Kiraranokiwami’, ‘Mihong’, ‘Misshong’, ‘Soomee’, ‘Suhong’, and ‘Sun Gold’). Pro content was analyzed using the ninhydrin method and related gene expressions were examined using quantitative real-time RT-PCR. While cold hardiness of 10 peach cultivars decreased, Pro contents of those increased during the deacclimation. Notably, at the same time, expression of P5CS (Δ1-pyrroline-5-carboxylatesynthase) decreased in 10 peach cultivars, whereas expressions of P5CR (Δ1-pyrroline-5-carboxylatereductase) and OAT (ornithine-δ-aminotransferase) increased. Our results demonstrate that Pro responds positively to higher temperature in the shoots of 10 peach cultivars and expression of both P5CS and P5CR genes could show contrasting patterns during the deacclimation. Furthermore, our results suggest that ornithine pathway, which has been suggested to be important during seedling development, could serve as an alternative pathway in Pro synthesis process during the deacclimation in peach.
Fabrication of suspended graphene field-effect transistors by the sandwich method
Shin Hyunsuk,Lee Sungbae 한국물리학회 2023 Current Applied Physics Vol.48 No.-
A novel fabrication technique that can be used for making a series of suspended graphene field-effect transistors on Si-substrate is discussed. The electrical properties of graphene channel can be significantly degraded by defects and chemical residues between graphene and substrate. To minimize electrical degradation, a method of physically suspending graphene from the substrate has been considered while maintaining its structural integrity. To address this problem, we employed a sandwich method to fabricate a suspended GFET, realizing 76% device fabrication yield that is higher than those realized by the other methods. Furthermore, the degradation of electrical properties due to external factors decreased. As our method has a mechanically stable structure, it can be imposed to make electrical devices with various two–dimensional (2D) materials. Our method can also be applied to the engineering of future devices in various applications because a large amount of electrically clean samples can be manufactured at once.
Hyunsuk Shin,Sewon Oh,Keumsun Kim,Youngjae Oh,Seolah Kim,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Changes in cold hardiness were confirmed with relative electrolyte leakage (REL) method in the shoots of two peach cultivars (Prunus persica Janghowon Hwangdo and Odoroki) during cold acclimation and deacclimation. Changes in proline (Pro) content and related gene expressions were also analyzed. Particularly, transcript accumulations of P5CS and P5CR were examined using quantitative real-time RT-PCR. REL in the shoots of two peach cultivars was significantly different during the entire experimental period. Cold hardiness of two cultivars increased gradually to December 2012, and then decreased to April 2013, whereas Pro contents of ‘Janghowon Hwangdo’ and ‘Odoroki’ were reduced from the beginning of the experiment to February 2012 and then increased in the spring. Interestingly, P5CS gene encoding an enzyme, which catalyzes conversion from glutamic acid (Glu) into glutamic-γ-semialdehyde (GSA) in the first step of Pro pathway, showed the contrasting patterns with Pro contents of two cultivars. On the other hand, P5CR gene encoding an enzyme, which catalyzes conversion from Δ1-pyrroline-5-carboxylate(P5C)intoPro in the final step of Pro pathway, showed the similar patterns to Pro contents in two cultivars. Our results demonstrate that Pro responds negatively to low temperatures in the shoot of different peach cultivars, including the supplemental 10 peach cultivars, and expression of both P5CS and P5CR genes could show contrasting patterns from each other. Our results suggest that identification of both P5CS and P5CR genes are required necessarily for accurate analysis of Pro biosynthesis because Pro accumulation is affected more by expression of P5CR gene.