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Availability of Novel Pear SSR Markers delivered from ‘Golden Delicious’ Apple Genomic Sequences
Jungyeon Won,Youngjae Oh,Seolah Kim,Hyunsuk Shin,Sewon Oh,Cheol Choi,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Korea is a origin of three basic species, P. ussuriensis, P. pyrifolia and P. fauriei. Genetic relationship among Korean pear cultivars compared with their parents were also identified that they are closely related P. pyrifolia, P. ussuriensis and/or hybrid between two species. SSRs or Microsatellites are co-dominant and typically neutral inheritance showing high degree of polymorphism, large number of alleles per locus, abundance in genomes, and suitability for automation. SSR markers were developed in apple and pear where they were used for construction of genetic linkage maps, evaluation of the genetic diversity, cultivar identification, genotype identification, and in the determination of genetic relatedness. Many apple (Malus × domestica Borkh.) SSRs would be useful for genetic mapping in European and Asian pears in previous experiments and cross-species amplification was observed between apple and pear. The objectives of this study were to develop polymorphic SSR markers in ‘Whangkeumbae’ and ‘Minibae’, which were chosen as the representative cultivars of P. pyrifolia and P. ussuriensis in each among Korean pears, from ‘Golden Delicious’ genomic sequences generated by next generation sequencing technology and to evaluate the utility of the SSR markers based on ‘Golden Delicious’ sequences. Of 51 SSR markers, 18 were polymorphic in ‘Whangkeumbae’ and ‘Minibae’. The cross-species transportability of primers designed in ‘Golden Delicious’ sequences makes SSR markers more useful, given the current high level of investment in mapping the genomes of related Rosaceae.
Won, Jungyeon,Chung, So Young,Kim, Seung Beom,Byun, Boo Hyeong,Yoon, Yoo Sik,Joe, Cheol O Korean Society for Molecular Biology 2006 Molecules and cells Vol.21 No.2
<P>The effect of poly(ADP-ribosyl)ation on the stability of p53 in SK-HEP1 cells treated with UV light was examined. Intracellular levels of p53 increased in cells treated with a low dose of UV light (20 J/m2), whereas they increased but then declined after a higher dose of UV (100 J/m2). Intracellular levels of p53 in the UV treated SK-HEP1 cells were dependent on the UV dose. Use of proteasome inhibitors revealed that p53 is degraded by proteasomal proteolysis after high doses of UV light. We present evidence that, at low doses, poly(ADP-ribose)polymerase (PARP) poly(ADP-ribosyl)ates p53 and protects it from proteasomal degradation before caspase-3 is activated, whereas at high doses the cells undergo UV induced apoptosis and PARP is cleaved by caspase-3 before it can protect p53 from degradation. Destabilization of p53 by cleavage of PARP may be important in cell fate decision favoring apoptosis.</P>
Sewon Oh,Hyunsuk Shin,Jungyeon Won,Youngjae Oh,Seolah Kim,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Recent climate changes due to global warming are gradually introducing adverse circumstances for winter survival of temperate fruit trees. Particularly, late winter or early spring thaws followed by hard freezes can cause severe injury to deacclimated flower buds. Thus, the selection of later or slower deacclimating cultivars is needed to avoid frost injury in late winter or early spring. This study was performed to investigate relationship between cold hardiness and bud development under an experimental deacclimation condition for 10 Prunus persica cultivars (Aikawanakajima, Daewol, Izumi Hakuto, Janghowon Hwangdo, Kiraranokiwami, Mihong, Misshong, Soomee, Suhong, and Sun Gold). The rate of deacclimation was not correlated with hardiness before the deacclimation treatment. On the other hand, a strong positive correlation was found between cold hardiness and stage of bud opening.
Youngjae Oh,Seolah Kim,Hyunsuk Shin,Jungyeon Won,Sam-Seok Kang,Yoon-Kyeong Kim,Cheol Choi,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Single nucleotide polymorphisms (SNPs) are the most frequent type among variations found in genomic regions and are valuable markers for genetic mapping, genetic diversity studies and association mapping in plants. There are three basic species known as Korean native which are Pyrus ussuriensis, P. pyrifolia, and P. fauriei. Genetic relationship among Korean pear cultivars compared with their parents was identified that they are closely related P. pyrifolia, P. ussuriensis and/or hybrids between two species. Lack of genetic resources, including molecular markers to study pears are very severe. Recently developed next generation sequencing (NGS) platforms offer opportunities for high-throughput and inexpensive genome sequencing and rapid marker development. The objective of this study was to develop polymorphic SNP markers in ‘Whangkeumbae’ and ‘Minibae’, which were chosen as the representative cultivars of P. pyrifolia and P. ussuriensis × pyrifolia in each among Korean pears, using genomic sequences generated by NGS technology. In this study, more than 18.6 Gbp and 15.8 Gbp sequences were obtained from NGS of ‘Whangkeumbae’ and ‘Minibae’, respectively. ‘Whangkeumbae’ and ‘Minibae’ contained 2,712,288 and 2,747,224 SNPs, respectively. In SNPs validations between ‘Whangkeumbae’ and ‘Minibae’, the number of polymorphic SNPs were 2,516,438 and non-polymorphic SNPs were 1,179,391. For HRM primer design, 2,125,479 HRM candidate primers were obtained from polymorphic SNPs and 343,731 SNP primers were developed. This study shows that the utility of NGS technology to discover efficiently a large number of SNPs and SNP primers can provide valuable information in the genome study of Pyrus spp.
Youngjae Oh,Seolah Kim,Hyunsuk Shin,Jungyeon Won,Sam-Seok Kang,Yoon-Kyeong Kim,Daeil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Currently, the type of short insertions and deletions (InDels) polymorphisms are increasingly focused in genomic research. InDels have been known as a source of genetic markers that are widely spread across the genome. Genetic relationship among Korean pear cultivars compared with their parents was also identified that they are closely related P. pyrifolia, P. ussuriensis and/or hybrids between two species. Lack of genetic resources including molecular markers has made it difficult to study pears severely. Recently developed next generation sequencing (NGS) platforms offer opportunities for high-throughput and inexpensive genome sequencing and rapid marker development. The main goal of this study was to develop polymorphic InDel markers in ‘Whangkeumbae’ and ‘Minibae’, which were chosen as the representative cultivars of P. pyrifolia and P. ussuriensis × pyrifolia in each among Korean pears using genomic sequences generated by NGS technology. In this study, more than 18.6 Gbp and 15.8 Gbp sequences were obtained from NGS of ‘Whangkeumbae’ and ‘Minibae’, respectively. ‘Whangkeumbae’ contained 197,210 InDels and 197,272 InDels in ‘Minibae’. In InDels validations between ‘Whangkeumbae’ and ‘Minibae’, the number of polymorphic InDels were 149,338 and non-polymorphic InDels were 122,572. For InDel primer set designing, 11,308 of primers were designed from polymorphic InDels and 10,919 of InDel primers were recommended. The study shows that the utility of NGS technology to design amount of efficient InDels and the developed InDel primers will be used for genetic mapping, breeding by marker assisted selection (MAS) and QTL mapping of Korea native pear as well as further genetic studies.
Electric Field-Triggered Metal-Insulator Transition Resistive Switching of Bilayered Multiphasic VOX
Seokjae Won,이상연,Jungyeon Hwang,Ju-Cheol Park,서형탁 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.1
Electric field-triggered Mott transition of VO2 for next-generationmemory devices with sharp and fast resistance-switching response isconsidered to be ideal but the formation of single-phase VO2 bycommon deposition techniques is very challenging. Here, VOx filmswith a VO2-dominant phase for a Mott transition-based metal-insulatortransition (MIT) switching device were successfully fabricated by thecombined process of RF magnetron sputtering of V metal andsubsequent O2 annealing to form. By performing various materialcharacterizations, including scanning transmission electron microscopyelectronenergy loss spectroscopy, the film is determined to have abilayer structure consisting of a VO2-rich bottom layer acting as theMott transition switching layer and a V2O5/V2O3 mixed top layeracting as a control layer that suppresses any stray leakage current andimproves cyclic performance. This bilayer structure enables excellentelectric field-triggered Mott transition-based resistive switching of Pt-VOx-Pt metal-insulator-metal devices with a set/reset current ratioreaching ~200, set/reset voltage of less than 2.5 V, and very stable DCcyclic switching upto ~120 cycles with a great set/reset current andvoltage distribution less than 5% of standard deviation at roomtemperature, which are specifications applicable for neuromorphic ormemory device applications.