MicroRNA expression profiling of adult hippocampal neural stem cells upon cell death reveals an autophagic cell death-like pattern

Park, Sujeong,Lee, Steven Hyun Seung,Lee, Won Il,Choi, Rachelle,Kim, Seong Who,Woo, Ha-Na,Lee, Heuiran Elsevier 2019 Biochemical and biophysical research communication Vol.509 No.3

<P><B>Abstract</B></P> <P>Adult hippocampal neural (HCN) stem cells promptly undergo irreversible autophagic cell death (ACD) if deprived of insulin in culture. Small, non-coding microRNAs (miRNA) play an important role in regulating biological processes, including proliferation and cell death. However, there have been no reports thus far regarding miRNA involvement in the induction of adult HCN stem cell death under insulin-deprived conditions, for which we performed a microarray-based analysis to examine the expression signature of miRNAs in adult rat HCN stem cells. Three independent specimens per culture condition either with or without insulin were prepared and a miRNA microarray analysis carried out. A total of 12 exhibited significantly altered expression levels upon cell death due to the absence of insulin when compared to HCN stem cells cultured with insulin present (cut-off limit; p < 0.05 and fold-change >1.3) The resulting volcano plot showed that, among these miRNAs, seven were upregulated and five were downregulated. The upregulated miRNAs were capable of modulating HCN stem cell death. Caspase-3 activity analysis, LC3 conversion, and TEM of autophagosome formation consistently suggested that ACD, not apoptosis, was most likely the mechanism affecting HCN cell death. As such, we have come to term these miRNAs, “HCN stem cell-specific autophagic cell death regulators.” Taken together, our data suggest that the miRNA expression profile of HCN stem cells is altered during ACD occurring due to insulin deprivation and that differentially expressed miRNAs are involved in HCN stem cell viability. Detailed explorations of the underlying mechanisms regarding HCN stem cell viability modulation by these miRNAs would be beneficial in further understanding the physiological features of adult HCN stem cells and are currently being investigated.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Insulin deprivation leads to autophagic cell death of hippocampal neural (HCN) stem cells. </LI> <LI> Expression pattern analysis of autophagic HCN stem death shows 7 upregulated microRNAs. </LI> <LI> Upregulated microRNAs induce autophagic HCN stem cell death under normal conditions. </LI> <LI> They have been termed, “HCN stem cell-specific autophagic cell death regulators”. </LI> </UL> </P>

Metabolic Engineering of Yeast for Ethanol Production by CO₂-Fixation

Sujeong PARK,Deokyeol JEONG,Jongbeom PARK,Suhyeung KIM,Soo Rin KIM 한국생물공학회 2022 한국생물공학회 학술대회 Vol.2022 No.4

Loss of Acot12 contributes to NAFLD independent of lipolysis of adipose tissue

Sujeong Park,Jinsoo Song,In-Jeoung Baek,Kyu Yun Jang,Chang Yeob Han,Dae Won Jun,Peter K. Kim,Brian Raught,Eun-Jung Jin 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

In this study, we hypothesized that deregulation in the maintenance of the pool of coenzyme A (CoA) may play a crucial role in thepathogenesis of nonalcoholic fatty liver disease (NAFLD). Specific deletion of Acot12 (Acot12−/−), the major acyl-CoA thioesterase,induced the accumulation of acetyl-CoA and resulted in the stimulation of de novo lipogenesis (DNL) and cholesterol biosynthesisin the liver. KEGG pathway analysis suggested PPARα signaling as the most significantly enriched pathway in Acot12−/− livers. Surprisingly, the exposure of Acot12−/− hepatocytes to fenofibrate significantly increased the accumulation of acetyl-CoA andresulted in the stimulation of cholesterol biosynthesis and DNL. Interaction analysis, including proximity-dependent biotin identification (BioID) analysis, suggested that ACOT12 may directly interact with vacuolar protein sorting-associated protein 33A (VPS33A) and play a role in vesicle-mediated cholesterol trafficking and the process of lysosomal degradation of cholesterol inhepatocytes. In summary, in this study, we found that ACOT12 deficiency is responsible for the pathogenesis of NAFLD through the accumulation of acetyl-CoA and the stimulation of DNL and cholesterol via activation of PPARα and inhibition of cholesterol trafficking.

Expression of Heterologous Bacterial CO₂-fixation Pathway in Yeast

Sujeong PARK,Deokyeol JEONG,Suhyeung KIM,Bonbin GU,Soo Rin KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Unpredictable climate change caused by global warming is threatening humanity and the ecosystem. To solve this problem, more than 110 countries and international organizations have agreed to reduce carbon dioxide (CO₂) emissions. Recently various industrial processes such as bioethanol and chemical production rely on yeast fermentation. In the yeast fermentation process, CO₂ emission into atmosphere is unavoidable. Therefore, expressing CO₂ fixation pathway in yeast would decrease the CO₂ emission during the process. The purpose of this study is to develop a S. cerevisiae strain that can utilize CO₂ generated during the fermentation. First, we constructed xylose oxidoreductase pathway to produce the precursor of CO₂ fixation pathway. Then, heterologous genes related to CO₂ fixation pathway are introduced by the CRISPR/Cas9 genome editing technology. We confirmed the soluble expression of ribulose-1,5-bisphosphate carboxylase/oxygenase. Moreover, we observed increased ethanol yield through the fermentation. These results suggest that expressing CO₂ fixation pathway in S. cerevisiae could be a strategy to alleviate the global warming.

[Vascular Surgery] Portomesenteric Vein Reconstruction by End-to-end Anastomosis or Interposition with Allograft in Pancreatic Cancer Surgery

Sujeong Lee,Yangjin Park,Jinseok Heo,Inwong Han,Sanghyun Shin,Jaeberm Park,Gyuseong Choi,Kyowon Lee,Minkyu Kim,Shinseok Yang 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.11

UX 디자인 프로세스를 이용한 안전 귀가 셉티드 디자인

Sujeong Park,Daeun Jeong,TaeKyun Kim 한국서비스디자인학회 2019 서비스디자인융합연구 Vol.3 No.1

본 연구는 밤길 귀가 시 여성을 타겟으로 한 범죄 실태와 문제점을 도출하고, 이에 대한 해결책으로 휴대용 드론을 활용한 서비스를 개발하는데 그 목적이 있다. 밤에 홀로 귀가하는 여성을 타겟으로 하는 성범죄 건수는 매해 늘어나고 있다. 이를 해결하기 위해 CCTV 설치를 늘렸지만 사각지대라는 한계가 있으며 주로 범죄 발생 후 증거 수집에 쓰인다. 호신용품과 기타 안전귀가 어플리케이션은 범죄 발생 후에만 사용될 뿐, 범죄를 예방하지 못한다. 즉, 기존의 안전귀가를 위한 제품과 서비스는 잠재적 범죄에 대한 직접적인 예방책을 제공하지 못한다. 이를 해결하기 위해 어플리케이션과 연동하여 사용자를 에스코트하는 휴대용 드론을 제안한다. 이 드론을 사용함으로써 잠재적 범죄자에게 사용자가 보호 하에 있다는 것을 가시적으로 보여준다. 이는 사용자가 범죄의 타겟이 되는 것을 막을 수 있다는 점에서 기존의 예방책과 차별화된다. 드론이 사용자를 촬영하며 따라오고, 앱으로 사이렌과 경광등을 작동시켜 위협 상황에서 범죄자의 범죄 심리를 억압한다. 또한 드론을 통해 경찰이 사용자의 위치를 쉽게 파악할 수 있다. 이와 같이 밤길에 발생할 수 있는 여러 가지 직접적, 간접적 범죄 상황의 시나리오 분석을 통해 드론을 활용한 효과적인 서비스를 제안하고 드론을 디자인하였다. This study aimed to develop a women s safety service using a portable drone based on derived problems from crimes against women on their way home at night. The number of sexual crimes has increased every year. To protect women who come back home late at night against the crime, CCTVs were installed on streets, however they just offer a video as a proof after the crime happened. Self-defense products and other applications for women s safety cannot prevent crime before it happens but only are used after it already happened. In other words, existing products and services for women s safety do not offer preventive measures. To solve the problem, the study proposes a portable drone escorting a user through linking to an application. By using this drone, it represents visually that a user is under protection. It provides a differentiated preventive service in the respect that it keep a user from being a victim of crime. The drone shoots a video above a user and she can operate a siren and flash lights, using an application. The application also provides a simple and convenient way to call 911. The drone is designed through the analysis of various scenarios about both direct and indirect threatening situations and provides an effective service in preventing crime.

Synthesis and in vitro Assay of New Triazole Linked Decursinol Derivatives Showing Inhibitory Activity against Cholinesterase for Alzheimer’s Disease Therapeutics

Park, Jung-Youl,Shin, Sujeong,Park, Kyoung Chan,Jeong, Eunju,Park, Jeong Ho Korean Chemical Society 2016 대한화학회지 Vol.60 No.2

With the goal of developing Alzheimer’s disease therapeutics, we have designed and synthesized new triazole linked decursinol derivatives having potency inhibitory activities against cholinesterase [acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE)]. Since inhibition of cholinesterase (ChE) is still considered to be one of the most effective targets to treat AD patients, many new classes of ChE inhibitors have been synthesized. In an effort of identifying new type of cholinergic drug, decursinol derivatives 11-17 have been synthesized between decursinol and other biological interesting compounds such as lipoic acid, polyphenols, etc by using the click reaction and then evaluated their biological activities. Compound 12 (IC₅₀ = 5.89 ± 0.31 mM against BuChE) showed more effective inhibitory activity against BuChE than galantamine (IC₅₀ = 9.4 ± 2.5 mM). Decursinol derivatives can be considered a new class inhibitor for BuChE and can be applied to be a novel drug candidate to treat AD patients.

Lifetime Analysis for Comparing POCl₃ Diffused Emitter Doping Characteristics in p-Type Silicon Solar Cells Using QSSPC

Park, HyunJung,Kim, Soo Min,Jeong, Sujeong,Shin, Seung Hyun,Park, Hyomin,Kang, Yoonmook,Lee, Hae-Seok,Kim, Donghwan American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.7

<P>Analysis of the emitter property of solar cells is important because the emitter doping characteristics can affect the surface recombination velocity, contact resistance, emitter saturation current density, and cell efficiency. To analyze the emitter quality, we used the following methods: the four-point probe method, quasi-steady-state photoconductance (QSSPC), and secondary ion mass spectroscopy (SIMS). The four-point probe method is used to measure the doping dose in the emitter. Using QSSPC, we can characterize the emitter quality, including the lifetime of the emitter, and using SIMS, we can measure the concentration of dopants as a function of depth in the emitter. However, SIMS measurement is destructive and limited to the measurement of planar surface wafers. To solve this problem, we investigated the relationship between the minority carrier lifetime and the emitter doping profile using the QSSPC. The relationship between the lifetime and emitter doping profile showed that the lifetime of the emitter decreases as the emitter doping concentration increases. From this result, we performed a lifetime analysis for differently doped POCl3-diffused emitters. The results obtained using the theoretical model for the lifetime agreed with experimental SIMS measurement results, indicating that the model can be used as a quantitative model for comparing emitter doping characteristics.</P>

Effects of Chamaecyparis Obtusa Essential Oil on the Autonomic Nervous System

Park, Sah-Hoon,Jeong, Han-Seong,Jang, Sujeong,Kim, Seong Jin,Park, Jong-Seong The Basic Science Institute Chosun University 2019 조선자연과학논문집 Vol.12 No.3

The purpose of this study is to explore the effects of Chamaecyparis obtusa essential oil on the activity of autonomic nervous system. Heart rate variability (HRV), as an indicator of autonomic nervous system activity and blood pressure were measured before and after inhalation of Chamaecyparis obtusa essential oil. The systolic blood pressure was decreased by inhalation of Chamaecyparis obtusa essential oil (p<0.05). The diastolic blood pressure was not changed significantly by Chamaecyparis obtusa. High frequency (HF) power level was not changed but High frequency/Low frequency (HF/LF) ratio was decreased by Chamaecyparis obtusa essential oil (p<0.05), meaning that parasympathetic nervous system activity was not affected but sympathetic nervous system activity was decreased. These results indicate that Chamaecyparis obtusa essential oil has a modulatory effect on the autonomic nervous system activity.

Comparative Analysis of Genetic Variation and Gene Flow of Nilaparvata lugens (Stål) by using Microsatellite Markers

Sujeong Min,Chang-Gyu Park,Si-Woo Lee,Yoo-Han Song 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

We developed and characterized five polymorphic microsatellites of Nilaparvata lugens from hybridization method using biotin enrichment strategy and two polymorphic microsatellites from Next Generation Sequencing. Also 11 microsatellites that developed by Sun et al. (2011) are employed to carry out genetic analysis of N. lugens in Southeast Asia. The number of alleles per locus ranged from 2 to 12 with an average of 4.63 alleles per locus. The mean observed heterozygosity of the eleven populations ranged from 0.031 to 0.938 and the expected hetetrozygosity ranged from 0.031 to 0.881. Signifiant genetic differentiation was detected among the three N. lugens populations as the FST ranged from 0.028 (Cheong Do and Ha Long) to 0.161 (CH and BN). The results of microsatellite marker suggested that found N. lugens migrated to Korea at least two times in different period or once. Genetic distance of N. lugens between Korea and Hi Pong were mostly closed and genetic distance of Ha Long and HCM were relatively closed. In this study, development of microsatellites should facilitate the study of future population genetics of N. lugens, and eventually elucidate the route of N. lugens migration to Korea. Thus, combining satisfactory microsatellite markers and intensive surveillance methods in paddy field could be easy to understand to the N. lugens migration mechanism.