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Surface Imaging of Barley Aleurone Cell by Atomic Force Microscopy
Kwang-Woon Huh,Seung-Hwan Kim,Hyun-Hwoi Ku,Byung-Moo Lee,Jae Yoon Kim,Yong-Won Seo,Tae Wan Kim 韓國作物學會 2004 한국작물학회지 Vol.49 No.1
To observe and analysis ultra-microscopically barley aleurone cell surface, atomic force microscope (AFM) was used. Seed coat of early maturing germplasm, eam9, was dehulled and scanned by non-contact mode. We have obtained the high resolution topographic 3-dimensional image of barley aleurone layer with high resolution. These images showed the membrane proteins in barley aleurone cell. One channel protein and numerous peripheral or integral proteins were detected in a area of 100 ~mu~textrmm2 . Furthermore, we found that their widths were ranged from 50 to 750nm and lengths from 0 to 66 ~mu~textrmm . The thickness of aleurone layer was measured by scanning electron microscope. The thickness at early developmental stage was about 16 and then the aleurone cell enlarged upto 57 ~mu~textrmm ~mu~textrmm at least until 42 days after anthesis. In this study, we firstly reported on the ultrastructural AFM analysis of living aleurone cell as a biological specimen. It was clearly suggested that AFM will become an powerful tool for probing both the structural properties of biological samples
Surface Imaging of Barley Aleurone Cell by Atomic Force Microscopy
Kim, Tae-Wan,Huh, Kwang-Woon,Kim, Seung-Hwan,Ku, Hyun-Hwoi,Lee, Byung-Moo,Kim, Jae-Yoon,Seo, Yong-Won The Korean Society of Crop Science 2004 Korean journal of crop science Vol.49 No.1
To observe and analysis ultra-microscopically barley aleurone cell surface, atomic force microscope (AFM) was used. Seed coat of early maturing germplasm, eam9, was dehulled and scanned by non-contact mode. We have obtained the high resolution topographic 3-dimensional image of barley aleurone layer with high resolution. These images showed the membrane proteins in barley aleurone cell. One channel protein and numerous peripheral or integral proteins were detected in a area of 100 $\mu\textrm{m}^2$. Furthermore, we found that their widths were ranged from 50 to 750nm and lengths from 0 to 66 $\mu\textrm{m}$. The thickness of aleurone layer was measured by scanning electron microscope. The thickness at early developmental stage was about 16 and then the aleurone cell enlarged upto 57 $\mu\textrm{m}$${\mu}{\textrm}{m}$ at least until 42 days after anthesis. In this study, we firstly reported on the ultrastructural AFM analysis of living aleurone cell as a biological specimen. It was clearly suggested that AFM will become an powerful tool for probing both the structural properties of biological samples.
혈액투석 중인 만성 신부전 환자에서 TT Virus의 감염률과 임상적 의의
이용욱,허우성,도재혁,백승운,최문석,김소정,이준행,고광철,이풍렬,이종철,최규완,박상종,이준혁,김재준,오하영,임윤정 대한소화기학회 2001 대한소화기학회지 Vol.37 No.1
Background/Aims: TT virus (TTV) is a unenveloped, single-stranded and circular DNA virus isolated from the serum of a patient with posttransfusion hepatitis of unknown etiology. We evaluated the prevalence, risk factors, and clinical significance of TTV in patients with chronic renal failure(CCRF) undergoing maintenance hemodialysis (HD). Methods: We examined TTV DNA in serum of HD-undergoing patients and healthy controls using the nested polymerase chain reaction. Results: TTV DNA was detected in 15 (20.0%) of 75 HD-undergoing patients and 10 (13.2%) of 76 healthy controls (p$gt;0.05). The prevalence of TTV did not differ according to the duration of HD or transfusion history of the patients. The prevalence of TTV was higher in IgG anti-HBc positive patients than IgG anti-HBc negative patients (27.5% vs. 4.2%, p=0.03). There was no relationship between TTV infection and liver diseases. Conclusions: The prevalence of TTV infection in CRF patients undergoing HD was similar with that of healthy controls. These results suggest that TTV infection may share the route of transmission with HBV infection in adults.
비가림 조건에서 시비에 대한 논담수 중 총질소 및 총인 농도 반응
정재운,최우정,윤광식,김한용,곽진협,임상선,장남익,허유정,Jung, Jae-Woon,Choi, Woo-Jung,Yoon, Kwang-Sik,Kim, Han-Yong,Kwak, Jin-Hyeob,Lim, Sang-Sun,Chang, Nam-Ik,Huh, Yu-Jeong 한국관개배수위원회 2007 한국관개배수논문집 Vol.14 No.1
Temporal changes in total nitrogen (T-N) and phosphorus (T-P) concentrations in paddy floodwater in response to fertilization under rain-shielding pot and small-scaled field conditions were investigated. On the basis of the changing patterns, suggestions for the use of fertilization factors, such as days after fertilization, in developing models for the estimation of T-N and T-P loads from paddy fields were made. Total N concentration was susceptible to fertilization, showing a peak concentration right after fertilization followed by a decreasing pattern with the elapse of days after fertilization. The decreasing pattern of T-N concentration followed the first- order kinetics, indicating that the models are likely to be an exponential equation using days after fertilization as an independent variable. Comparison between the pot and field experiments conducted with soils different in soil fertility revealed that indigenous soil N concentration significantly affected T-N concentration, and this suggests that soil N status can be used as the second variable for the models. Meanwhile, temporal changes in T-P concentration did not respond to P fertilization as sensitively as T-N. In combination with other published results, our study suggests that rainfall intensity and other factors associated with farming activities that are likely to cause disturbance of soil particles containing P may be used as possible variables for the models.
Antioxidative Activities in Rice Leaves Exposed to Ozone
Lee Su Yeon,Sol Yun,Hyo Jin Lim,Jwa-Kyung Sung,Seon-Woong Hwang,Kwang Woon Huh,Tae Wan Kim 韓國作物學會 2004 Korean journal of crop science Vol.49 No.2
Rice (Oryza sativa L.) plants were cultivated to examine changes in antioxidative defence mechanism induced by elevated ozone levels. Catalase activities in tolerant Jinpumbyeo and susceptible Chucheongbyeo under ozone fumigation were reduced at 5 hrs and 3 hrs after ozone fumigation, respectively. With the increased ozone supply, peroxidase activity in Jinpumbyeo was steadily enhanced whereas in Chucheongbyeo it was not changed. Four SOD-isozymes were detected by NBT staining of native-PAGE. Two isozymes of them were obviously induced by ozone supply, particularly in Jinpumbyeo. The continuous ozone fumigation increased remarkably putrescine levels in leaves whereas it did not affect the levels of spermidine and spermine. In this study, it was implied that ozone in cell inhibits strongly diamine oxidase and thus promotes ethylene biosynthesis which will cause the senescence in rice plants.
영산강.섬진강 유역을 대상으로 한 논 오염부하 산정 단순회귀모형 적용
최우정,곽진협,정재운,윤광식,장남익,허유정,Choi, Woo-Jung,Kwak, Jin-Hyeob,Jung, Jae-Woon,Yoon, Kwang-Sik,Chang, Nam-Ik,Huh, Yu-Jeong 한국농공학회 2007 한국농공학회논문집 Vol.49 No.1
Simple regression models for pollutants load estimation of paddy developed by the Ministry of Environment in 1995 were tested with the data (T-N, T-P, $COD_{Mn}$, and SS) collected from Yeongsan and Seomjin river watersheds, and improvement measures were suggested. Overall, the simulated values showed a great difference from the measured values except for T-P according to the statistical analyses (RMSE, root mean square error; RMAE, root mean absolute error; RB, relative bias; EI, efficiency index). Such difference was assumed due to the fact that the models use only hydrologic factors (quantity factor) associated with precipitation and run-off as input parameters, but do not consider other factors which are likely to affect pollutant concentration (quality factor) including days after fertilization. In addition, in terms of accessibility of the models, some parameters in the models such as run-off depth and run-off amount which can not be obtained from the weather database but should be collected by on-site measurements need to be replaced with other variables.