성인 남자의 식습관과 혈중 지질 농도에 관한 연구

신승렬,황보진향,박동철 慶山大學校 1997 論文集 Vol.15 No.1

This paper was carried out to study on the food & daily habit score and lipid contents in serum of adult man as basal research for research on the effects of food & daily habits on the lipid contents in serum of adult man. The number of normal, dangerous leval and hypercholesterolemia was each 133(60.5%), 62(28.2%) and 25(11.3%) of adult man. respectively. The mean age. weight. Broca's index(RBW) and Quetelet index(BMI) was 48.4 9.5. 67.7 10.2. 109.1 14.3, 23.6 3.0. respectively. The numbers of good food habits was 5.4 percentage, but those of bad food habits was 35.5 percentage, It was appeared to bad the mode of dietary and hormony of food. The contents of trigryceride and cholesterol in serum was 115.8 and 192.4mg/dℓ, respectively. The numbers of triglyceride contents(90∼120mg/dℓ in serum was 53(24.1%) of the subjects of research, and those of triglyceride contents (150-210mg/dℓ) in serum was 133(60.5%) of the adult man.

Carbon disulfide induces mitochondria-mediated apoptosis in Sertoli-germ cells coculture

Wei Wang,Zhen Zhang,Yinsheng Guo,Yu Dong,Xiaoyu Huang1,Yijun Zhou,Guoyuan Chen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Carbon disulfide (CS2), a common organic solvent, induces a variety of adverse effects in the male reproductive system. In this study, we investigated the cytotoxicity, ultrastructural changes, and potential apoptotic induction mechanisms of CS2 in mixed cultures of Sertoli and germ cells. Sertoli and germ cells were cocultured and treated with CS2 for 24 h. Growth rates were noted, and apoptotic cells were identified by Hoechst 33258 staining. Ultrastructure changes were observed via transmission electron microscopy (TEM). Mitochondrial membrane potential and expressions of apoptosis-related factors (cytochrome c, Bax, Bcl- 2, caspase-3 and caspase-9) were examined by JC-1 staining, western blot, and real-time PCR. The results showed that CS2 treatment was associated with reduced growth rates of Sertoli-germ cells. Ultrastructure changes in Sertoli-germ cells treated with CS2 were typical of apoptosis. In addition, CS2 treatment depolarized mitochondrial membrane potential, upregulated Bax levels and downregulated Bcl-2 levels, released cytochrome c from the mitochondrial intermembrane space to the cytosol, and triggered mitochondria-mediated apoptosis. Subsequently, caspase-9 and caspase-3 were activated, resulting in Sertoli-germ cells apoptosis. The above data suggest that CS2 has adverse effect on the viability of Sertoli-germ cells and induces apoptosis through mitochondrial pathway.

C-type natriuretic peptide attenuates renal osteodystrophy through inhibition of FGF-23/MAPK signaling

Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.

ECG PVC Classification Algorithm based on Fusion SVM and Wavelet Transform

Huang Dong,Liao Zhengquan,Li Changbin,Li Dan,Huang Wendong 보안공학연구지원센터(IJSIP) 2015 International Journal of Signal Processing, Image Vol.8 No.1

In the process of ventricular premature beat (PVC) and normal sinus rhythm (NSR) identification base on electrocardiogram (ECG), there exists problems like negative effect from ECG rhythm and low recognition rate. This paper proposes the electrocardiogram PVC classification algorithm based on support vector machine (SVM) and wavelet algorithm. The algorithm uses the wavelet transform to analyze ECG beating model, which is not influenced by the change of ECG waveform. The two feature sets respectively compose of statistical parameters of the wavelet coefficients and the selected wavelet coefficients. PVC and NSR are analyzed by using SVM. The experimental results show that this method improves the recognition rate of ECG.

Impacts of shape and height of building roof on airflow and pollutant dispersion inside an isolated street canyon

Huang, Yuan-Dong,Xu, Xuan,Liu, Ze-Yu,Deng, Ji-Tong,Kim, Chang-Nyung Taylor and Francis 2016 Environmental forensics Vol.17 No.4

A Hybrid Double Self-lift Switched-Coupled Inductor Quasi-Z-Source Inverter

Huang Dong,Xue Pengfei,Chen Guang,Zhang Chaozhong,Zhao Chang,Huang Yuchen,Ding Xinping,Zhang Min 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.4

A hybrid double self-lift switch-coupled-inductor quasi-Z-source inverter (HDSCL-qZSI) is proposed in this paper. The voltage boost capability is higher and the voltage and current stress on components are lower than the existing quasi-Z-source topologies. Owing to the strong current handling ability and step-down coupled-inductor, the magnetic elements are smaller than that of mSSCL-qZSI and SCL-qZSI whose structure are similar to that of HDCSL-qZSI. The operation principles, comparisons of voltage and current stress on components and the size of magnetic elements are analyzed theoretically. Finally, the eff ectiveness of the proposed HDSCL-qZSI was validated by a 1 kW prototype fabricated in the laboratory.

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

Huang Yuyuan,Dong Lingzhi,Gong Jian,Yang Jing,Lu Shan,Lai Xin-He,Jin Dong,Huang Qianni,Pu Ji,Liu Liyun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.10

Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20 54 N, 106°49 20 E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09 10 N, 102°04 39 E, October 2013) of South China, respectively. Optimal growth is obtained at 25–28°C (range, 4–32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5– 1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%; ANI, 91.9–92.1%). Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T. Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).

An Experimental on Filtration and Clogging of Geotextile Filters around Drain Pipes in Fine Tailings

Dong-dong Li,Xuan Cui,Jing-qi Huang,Ya-bing Han 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.5

Needle-punched nonwoven geotextiles have been used as filters for decades in mine drainage systems. But the physical clogging of geotextiles by fine particles has continued to receive increasing attention. In this study, the permeation characteristics of a drain pipe wrapped with geotextiles were investigated based on a new radial flow experiment apparatus, and particle size distribution (PSD), pore water pressure (PWP), and scanning electron microscopy (SEM) analyses were employed to identify the geotextile clogging mechanism. The geotextile cleaning methods after clogging were also discussed. The results showed that an exponential decay of hydraulic conductivity (K) with time under different flow conditions. Particles less than 30 μm migrated to the drainage pipe under the action of seepage forces, and a dense and thick cake layer was formed upstream of the geotextile filter. According to microscopic analysis, the clogging process of geotextiles was divided into three stages: pore blockage and cake formation, filter cake dynamic growth and cake layer filtration. Backwash cleaning is a good way to remove a filter cake layer on the surface of geotextiles, which can recover 60% of the hydraulic conductivity (K0).

Screening of Differentially Expressed Genes Related to Bladder Cancer and Functional Analysis with DNA Microarray

Huang, Yi-Dong,Shan, Wei,Zeng, Li,Wu, Yang Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Objective: The purpose of this study was to identify genes related to bladder cancer with samples from normal and disease cases by microarray chip. Methods: After downloading the gene expression profile GSE3167 from Gene Expression Omnibus database which includes 50 bladder samples, comprising 9 normal and 41 disease samples, differentially expressed genes were identified with packages in R language. The selected differentially expressed genes were further analyzed using bioinformatics methods. Firstly, molecular functions, biological processes and cell component analysis were researched by software Gestalt. Then, software String was used to search interaction relationships among differentially expressed genes, and hub genes of the network were selected. Finally, by using plugins of software Cytoscape, Mcode and Bingo, module analysis of hub-genes was performed. Results: A total of 221 genes were identified as differentially expressed by comparing normal and disease bladder samples, and a network as well as the hub gene C1QBP was obtained from the network. The C1QBP module had the closest relationship to production of molecular mediators involved in inflammatory responses. Conclusion: We obtained differentially expressed genes of bladder cancer by microarray, and both PRDX2 and YWHAZ in the module with hub gene C1QBP were most significantly related to production of molecular mediators involved in inflammatory responses. From knowledge of inflammatory responses and cancer, our results showed that, the hub gene and its module could induce inflammation in bladder cancer. These related genes are candidate bio-markers for bladder cancer diagnosis and might be helpful in designing novel therapies.

Microarray Analysis of the Hypoxia-induced Gene Expression Profile in Malignant C6 Glioma Cells

Huang, Xiao-Dong,Wang, Ze-Fen,Dai, Li-Ming,Li, Zhi-Qiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Hypoxia is commonly featured during glioma growth and plays an important role in the processes underlying tumor progression to increasing malignancy. Here we compared the gene expression profiles of rat C6 malignant glioma cells under normoxic and hypoxic conditions by cDNA microarray analysis. Compared to normoxic culture conditions, 180 genes were up-regulated and 67 genes were down-regulated under hypoxia mimicked by $CoCl_2$ treatment. These differentially expressed genes were involved in mutiple biological functions including development and differentiation, immune and stress response, metabolic process, and cellular physiological response. It was found that hypoxia significantly regulated genes involved in regulation of glycolysis and cell differentiation, as well as intracellular signalling pathways related to Notch and focal adhesion, which are closely associated with tumor malignant growth. These results should facilitate investigation of the role of hypoxia in the glioma development and exploration of therapeutic targets for inhibition of glioma growth.