Infection of Oligochaetes, Limnodrilus hoffmeisteri (Annelida: Oligochaeta), in the Nasal Cavity of a Chinese Man

Hongbin Liu,Zhenming Zhang,Guangping Huang,Xiaolong Gu,Chunmiao Wang,Yan Wang,Zhimin Lu 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.1

A Novel Shared Segment Protection Algorithm for Multicast Sessions in Mesh WDM Networks

Cai Lu,Hongbin Luo,Sheng Wang,Lemin Li 한국전자통신연구원 2006 ETRI Journal Vol.28 No.3

This paper investigates the problem of protecting multicast sessions in mesh wavelength-division multiplexing (WDM) networks against single link failures, for example, a fiber cut in optical networks. First, we study the two characteristics of multicast sessions in mesh WDM networks with sparse light splitter configuration. Traditionally, a multicast tree does not contain any circles, and the first characteristic is that a multicast tree has better performance if it contains some circles. Note that a multicast tree has several branches. If a path is added between the leave nodes on different branches, the segment between them on the multicast tree is protected. Based the two characteristics, the survivable multicast sessions routing problem is formulated into an Integer Linear Programming (ILP). Then, a heuristic algorithm, named the adaptive shared segment protection (ASSP) algorithm, is proposed for multicast sessions. The ASSP algorithm need not previously identify the segments for a multicast tree. The segments are determined during the algorithm process. Comparisons are made between the ASSP and two other reported schemes, link disjoint trees (LDT) and shared disjoint paths (SDP), in terms of blocking probability and resource cost on CERNET and USNET topologies. Simulations show that the ASSP algorithm has better performance than other existing schemes.

Preparation of anhydrous from red gypsum and effect of high strength gypsum on its properties

Changrong Liu,Lu Wang,Hongbin Tan,Faqin Dong,Xiaoling Ma,Feihua Yang 한양대학교 청정에너지연구소 2022 Journal of Ceramic Processing Research Vol.23 No.6

Red gypsum waste comes from titanium dioxide production by sulphuric acid method. Anhydrite was prepared from thewaste. The effects of calcined temperature on the properties of anhydrite were studied. The normal consistency of anhydritedecreased with the increase of calcined temperature, while the compressive strength firstly increase and then decrease. Theeffect of high strength gypsum on the properties of anhydrite was also studied. The normal consistency of sample decreasedwith the increase of high strength gypsum content, while the setting time firstly decrease and then increase, strength anddensity increased.

Measurement of TOF of fast neutrons with ²³⁸U target

Li, Meng,Guan, Yuanfan,Lu, Chengui,Zhang, Junwei,Yuan, Xiaohua,Duan, Limin,Yang, Herun,Hu, Rongjiang,He, Zhiyong,Wei, Xianglun,Ma, Peng,Gan, Zaiguo,Yang, Chunli,Zhang, Hongbin,Chen, Liang,Qiu, Tianli Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.6

We developed a Dual-PPACs detector for fast neutron measurements that consists of two sets of PPAC: conventional PPAC and fission PPAC. A²³⁸U(U₃O₈) coating is placed in the fission PPAC's anode, which is used as the neutrons conversion layer. An experiment was performed to measure neutron time-of-flight (TOF) in which ²⁵²Cf spontaneous fission source was used. An excellent time resolution of 164ps has been observed at 6 mbar in isobutene gas. With the excellent time resolution of Dual-PPACs detector, exact neutron energy can be extracted from the timing measurement. The experimental detection efficiency was 1.9 × 10^-7, consistent with the efficiency of 2.5 × 10^-7 given by a Geant4 simulation. Ultimately, the results show that the Dual-PPACs detector is a suitable candidate for measuring fast neutrons in the future CiADS system.

Interaction Between Serum/Glucocorticoid-Regulated Kinase 1 and Interleukin-6 in Chronic Rhinosinusitis

Lai Yuting,Hu Li,Yang Lu,Hu Xianting,Song Xiaole,Yang Jingyi,Li Hongbin,Chen Kun,Li Huabin,Wang Dehui 대한천식알레르기학회 2021 Allergy, Asthma & Immunology Research Vol.13 No.5

Purpose: Serum/glucocorticoid-regulated kinase 1 (SGK1) has recently emerged as a critical regulator of inflammatory diseases. In this study, we examined SGK1 expression and its possible pathogenic roles in chronic rhinosinusitis (CRS). Methods: Immunohistochemistry, western blotting, Bio-Plex assay, enzyme-linked immunosorbent assays, and quantitative real-time polymerase chain reaction were performed to assess protein and gene expression levels. The mRNA expression levels of SGK1 and interleukin-6 (IL-6) were extracted from a CRS database to perform correlation analysis. Stable cell lines with SGK1 overexpression (16HBE) and knockdown (A549) were constructed to investigate the interaction between SGK1 and IL-6 in vitro. Results: SGK1 exhibited strong cytoplasmic and nuclear staining in the epithelial layers and the lamina propria of nasal polyps (NPs) and in the mucosal tissues of CRS without nasal polyps (CRSsNP). The mRNA and protein expression levels of SGK1 and IL-6 were significantly increased in NPs and CRSsNP tissues, compared to control tissues. SGK1 phosphorylation was significantly greater in NPs than in CRSsNP tissues (P < 0.01). The mRNA levels of SGK1 and IL-6 were significantly correlated (P < 0.001, r = 0.649). Exposure to IL-6 significantly increased SGK1 expression in cultured dispersed NP cells, 16HBE cells, and A549 cells. IL-6 expression was significantly down-regulated in SGK1-overexpressing 16HBE cells (P < 0.01) and significantly up-regulated in SGK1-knockdown A549 cells (P < 0.05). Administration of GSK650394, a SGK1 inhibitor, significantly increased IL-6 self-induced mRNA expression in cultured dispersed NP cells and 16HBE cells. Conclusions: The interaction between SGK1 and IL-6 may play an anti-inflammatory role in IL-6-induced inflammation in the pathogenesis of CRS.

Expression profiling of the mitogen-activated protein kinase gene family reveals their diverse response pattern in two different salt-tolerant Glycyrrhiza species

Cao Aiping,Gao Ling,Wang Fei,Tong Xuechen,Xie Shuangquan,Chen Xifeng,Lu Tianxin,Shen Haitao,Liu Hailiang,Jin Xiang,Li Hongbin 한국유전학회 2022 Genes & Genomics Vol.44 No.7

Background: Mitogen-activated protein kinases (MPKs) play important role in response to environmental stress as crucial signal receptors or sensors. Our previous study indicated that salt stress acts as a positive factor to stimulate the production of pharmacodynamic metabolites in the medicinal plant Glycyrrhiza uralensis. Currently, little is known about the MPK gene family and their functions in the medicinal plant G. uralensis. Objective: Identification, comprehensive bioinformatic analysis, expression profiling, and response pattern under salt stress of the G. uralensis GuMPK gene family. Methods: Genome-wide investigation and expression profiling of the MPK gene family in G. uralensis, and their phylogenetic relationships, evolutionary characteristics, gene structure, motif distribution, promoter cis-acting element, and expression pattern under salt stress in two different salt-tolerant Glycyrrhiza species were performed. Results: A total of 20 G. uralensis GuMPK genes were identified and categorized into five groups, and had conserved gene structure and motif distribution. Expression profiling of GuMPK genes suggested their potentially diverse functions in plant growth and in response to phytohormones and environmental stress, particularly GuMPK1, 2, 5, and 10 as key components for G. uralensis in response to abiotic stress. Further expression analysis under NaCl treatment in two different salt-tolerant Glycyrrhiza species displayed the MPKs' different response patterns, emphasizing the role of MPK2, 5, 7, and 16 as potentially crucial genes for Glycyrrhiza to respond to salt stress. Conclusion: Our results provide a genome-wide identification and expression profiling of MPK gene family in G. uralensis, and establish the foundation for screening key responsive genes and understanding the potential function and regulatory mechanism of GuMPKs in salt responsiveness.

Enhanced anticorrosion performance of PPY-graphene oxide/PPY- camphorsulfonic acid composite coating for 304SS bipolar plates in proton exchange membrane fuel cell

Li Jiang,Junaid Ali Syed,Guoli Zhang,Yujie Ma,Jun Ma,Hongbin Lu,Xiangkang Meng 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-

The enhanced corrosion resistance with sustained conductivity are the prerequisites of stainless steelbipolar plates for practical application in proton exchange membrane fuel cell. Herein, we prepare aconductive polypyrrole-graphene oxide/polypyrrole-camphorsulfonic acid bilayer composite coating(PPY-GO/PPY-CSA) on 304 stainless steel bipolar plate by electrodeposition method. The electrochemicaltests are conducted in the simulated bipolar plates working environment, the potentiostatic polarizationresults imply that the PPY-GO/PPY-CSA composite coating offers stable corrosion resistance with lowpotentiostatic corrosion current density in comparison with the PPY-GO coating. The correspondingelectrochemical impedance spectroscopy measurements reveal that the PPY-GO/PPY-CSA compositecoating exhibits satisfactory conductivity and displays sustained anodic protection effect with superioranticorrosion performance during the 696 h of immersion. The excellent corrosion protection ability ofthe PPY-GO/PPY-CSA composite coating owing to its good adhesion strength, compact structure,satisfactory conductivity as well as the synergetic interaction between the two layers of the coating.

Ultrathin Two-Dimensional Atomic Crystals as Stable Interfacial Layer for Improvement of Lithium Metal Anode

Yan, Kai,Lee, Hyun-Wook,Gao, Teng,Zheng, Guangyuan,Yao, Hongbin,Wang, Haotian,Lu, Zhenda,Zhou, Yu,Liang, Zheng,Liu, Zhongfan,Chu, Steven,Cui, Yi American Chemical Society 2014 NANO LETTERS Vol.14 No.10

<P>Stable cycling of lithium metal anode is challenging due to the dendritic lithium formation and high chemical reactivity of lithium with electrolyte and nearly all the materials. Here, we demonstrate a promising novel electrode design by growing two-dimensional (2D) atomic crystal layers including hexagonal boron nitride (h-BN) and graphene directly on Cu metal current collectors. Lithium ions were able to penetrate through the point and line defects of the 2D layers during the electrochemical deposition, leading to sandwiched lithium metal between ultrathin 2D layers and Cu. The 2D layers afford an excellent interfacial protection of Li metal due to their remarkable chemical stability as well as mechanical strength and flexibility, resulting from the strong intralayer bonds and ultrathin thickness. Smooth Li metal deposition without dendritic and mossy Li formation was realized. We showed stable cycling over 50 cycles with Coulombic efficiency ∼97% in organic carbonate electrolyte with current density and areal capacity up to the practical value of 2.0 mA/cm<SUP>2</SUP>and 5.0 mAh/cm<SUP>2</SUP>, respectively, which is a significant improvement over the unprotected electrodes in the same electrolyte.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2014/nalefd.2014.14.issue-10/nl503125u/production/images/medium/nl-2014-03125u_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl503125u'>ACS Electronic Supporting Info</A></P>

Development of a multiplex qRT-PCR assay for detection of African swine fever virus, classical swine fever virus and porcine reproductive and respiratory syndrome virus

Yating Chen,Kaichuang Shi,Huixin Liu,Yanwen Yin,Jing Zhao,Feng Long,Wenjun Lu,Hongbin Si 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6

Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes. Therefore, a rapid and specific method is needed for the differential detection of these pathogens. Objectives: The aim of this study was to develop a multiplex real-time quantitative reverse transcription polymerase chain reaction (multiplex qRT-PCR) for the simultaneous differential detection of ASFV, CSFV, and PRRSV. Methods: Three pairs of primers and TaqMan probes targeting the ASFV p72 gene, CSFV 5′ untranslated region, and PRRSV ORF7 gene were designed. After optimizing the reaction conditions, including the annealing temperature, primer concentration, and probe concentration, multiplex qRT-PCR for simultaneous and differential detection of ASFV, CSFV, and PRRSV was developed. Subsequently, 1,143 clinical samples were detected to verify the practicality of the assay. Results: The multiplex qRT-PCR assay could specifically and simultaneously detect the ASFV, CSFV, and PRRSV with a detection limit of 1.78 × 100 copies for the ASFV, CSFV, and PRRSV, but could not amplify the other major porcine viruses, such as pseudorabies virus, porcine circovirus type 1 (PCV1), PCV2, PCV3, foot-and-mouth disease virus, porcine parvovirus, atypical porcine pestivirus, and Senecavirus A. The assay had good repeatability with coefficients of variation of intra- and inter-assay of less than 1.2%. Finally, the assay was used to detect 1,143 clinical samples to evaluate its practicality in the field. The positive rates of ASFV, CSFV, and PRRSV were 25.63%, 9.36%, and 17.50%, respectively. The co-infection rates of ASFV+CSFV, ASFV+PRRSV, CSFV+PRRSV, and ASFV+CSFV+PRRSV were 2.45%, 2.36%, 1.57%, and 0.17%, respectively. Conclusions: The multiplex qRT-PCR developed in this study could provide a rapid, sensitive, specific diagnostic tool for the simultaneous and differential detection of ASFV, CSFV, and PRRSV.

Enhanced corrosion resistance by polypyrrole and Ti3C2Tx-acrylic epoxy double-layer coating for 304SS bipolar plates of PEMFC

Haifeng Bian,Guoli Zhang,Qingxi Zhai,Yanyan Du,Yujie Ma,Beibei Yang,Shaochun Tang,Duan Bin,Xiangkang Meng,Hongbin Lu 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

Stainless steel (SS) is a promising material for designing bipolar plates (BPs), but their further applicationis limited by serious corrosion problems in the acidic environment. Ti3C2Tx is expected to be used for SSBPs coatings, while galvanic corrosion will occur after the damage of Ti3C2Tx coating. Herein, a PPY/Ti3C2Tx-AE double-layer coating (DC) is well-designed and prepared on 304SS BP, which is composedof an inner electropolymerized PPY layer and an outer Ti3C2Tx-acrylic epoxy layer. When tested in0.2 M HCl solution, the corrosion potential and corrosion current density of the DC are 38 mV and0.00927 lA cm2 respectively, which are superior to those of the PPY coating and the Ti3C2Tx coating. Moreover, the DC presents the best long-term stability among the three coatings. The excellent corrosionresistance is attributed to the barrier and anodic protection effects as well as the solution of galvanic corrosion. The new coating system provides a new insight into the design of DC coatings of SS BPs.