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- 저자 : Yating Chen (검색결과 4 건)
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Yating Chen,Kaichuang Shi,Huixin Liu,Yanwen Yin,Jing Zhao,Feng Long,Wenjun Lu,Hongbin Si 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.6
Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes. Therefore, a rapid and specific method is needed for the differential detection of these pathogens. Objectives: The aim of this study was to develop a multiplex real-time quantitative reverse transcription polymerase chain reaction (multiplex qRT-PCR) for the simultaneous differential detection of ASFV, CSFV, and PRRSV. Methods: Three pairs of primers and TaqMan probes targeting the ASFV p72 gene, CSFV 5′ untranslated region, and PRRSV ORF7 gene were designed. After optimizing the reaction conditions, including the annealing temperature, primer concentration, and probe concentration, multiplex qRT-PCR for simultaneous and differential detection of ASFV, CSFV, and PRRSV was developed. Subsequently, 1,143 clinical samples were detected to verify the practicality of the assay. Results: The multiplex qRT-PCR assay could specifically and simultaneously detect the ASFV, CSFV, and PRRSV with a detection limit of 1.78 × 100 copies for the ASFV, CSFV, and PRRSV, but could not amplify the other major porcine viruses, such as pseudorabies virus, porcine circovirus type 1 (PCV1), PCV2, PCV3, foot-and-mouth disease virus, porcine parvovirus, atypical porcine pestivirus, and Senecavirus A. The assay had good repeatability with coefficients of variation of intra- and inter-assay of less than 1.2%. Finally, the assay was used to detect 1,143 clinical samples to evaluate its practicality in the field. The positive rates of ASFV, CSFV, and PRRSV were 25.63%, 9.36%, and 17.50%, respectively. The co-infection rates of ASFV+CSFV, ASFV+PRRSV, CSFV+PRRSV, and ASFV+CSFV+PRRSV were 2.45%, 2.36%, 1.57%, and 0.17%, respectively. Conclusions: The multiplex qRT-PCR developed in this study could provide a rapid, sensitive, specific diagnostic tool for the simultaneous and differential detection of ASFV, CSFV, and PRRSV.
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Huie Chen,Haotian Guo,Xiaoqing Yuan,Yating Chen,Chao Sun 대한토목학회 2020 KSCE Journal of Civil Engineering Vol.24 No.9
The strength characteristics of unsaturated silty clay after the cooling and single freeze-thawcycle were studied by using Global Digital Systems Ltd. (GDS) triaxial test system aftercontrolling initial matric suction. The results demonstrate that, the initial matrix suction andfreezing process can strengthen the shear strength of the soil, which makes the stress-straincurve of the soil show certain strain hardening characteristics at different temperatures. whenthe temperature is negative (including 0°C), the curve has a flat transition section whosestrength does not change with deformation, and the flat transition section becomes shorter asthe temperature and confining pressure decrease. The strength of the soil at negativetemperature and the speed at which it reaches its peak value are much greater than those ofthe soil after positive temperature and single freeze-thaw cycle, and the contribution ofconfining pressure to the strength decreases with the decrease of temperature. The effect oftemperature change (15oC to -15oC) on soil strength is mainly reflected in the total cohesion,and the effect of freeze-thaw on the effective internal friction angle is more significant. Thestrength and deformation characteristics of soil under the freezing process and freeze-thawcycle are determined by the initial matric suction, the form of connection between soilparticles, the migration of unfrozen water, and the degree of water-ice phase transformation.
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Yating Wang,Jianping Ni,Cui Chen,Jinyong Peng,Hongbin Liu 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
High-yield pulp (HYP) is gaining increasing interest in papermaking, the presence of anionic trash hasbeen limiting its application in high-end paper products such as fine papers. In order to control the anionictrash ofHYP, the performances of three anionic trash catchers in terms of charge controland fiber fines andfiller retention enhancement were compared in a laboratory study. It was found that a poly-DADMACbased commercial product had the highest efficiency. The use of CPAM/Bentonite system resulted inhighest fiber fines and filler retention. The best anionic trash controlwas achieved when HYPwas treatedseparately.
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Fang Ting,Liu Suyi,Chen Liying,Ren Yating,Lu Dingqi,Yao Xinyi,Hong Tao,Zhang Xvfeng,Xie Zhimin,Yang Kepeng,Wang Xinchang 한국유전학회 2023 Genes & Genomics Vol.45 No.9
Background As a multisystemic autoimmune illness, the basic mechanisms behind the pathophysiology of systemic lupus erythematosus (SLE) remain poorly understood. Objective We aimed to investigate the possible significance of SLE’s DNA methylation and gain further insight into potential SLE-related biomarkers and therapeutic targets. Methods We used whole genome bisulfite sequencing (WGBS) method to analyze DNA methylation in 4 SLE patients and 4 healthy people. Results 702 differentially methylated regions (DMRs) were identified, and 480 DMR-associated genes were annotated. We found the majority of the DMR-associated elements were enriched in repeat and gene bodies. The top 10 hub genes identified were LCK, FYB, PTK2B, LYN, CTNNB1, MAPK1, GNAQ, PRKCA, ABL1, and CD247. Compared to the control group, LCK and PTK2B had considerably decreased levels of mRNA expression in the SLE group. Receiver operating characteristic (ROC) curve suggested that LCK and PTK2B may be potential candidate biomarkers to predict SLE. Conclusions Our study improved comprehension of the DNA methylation patterns of SLE and identified potential biomarkers and therapeutic targets for SLE.
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