Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

국어 매김말의 구조

홍양추 東亞大學校 國語國文學科 1978 국어국문학 Vol.2 No.-

In Korean, Determinatives is regarded as Endocentric Construction from the viewpoint of descriptive, but according to Trans-formation grammar, Determinatives is the Sentence that Constitue-nt Sentence is embedded to matrix sentence in deep structure. 1) Determinative is that determinatives is copied to nominal according to the feature of head noun and modify Head noun according to description and demonstration. 2) The determinative marker of predicates is composed of {ㄴ/은/는}{f/을}and modify according to action and descrition. In deep structure, determinative marker of predicates-causes Det-Transformation of by embedding constituent Sentence to matrix Sentence. If NP of matrix Sentence and NP of Constituent Sentence agree with each other, the determinative marker of predicates becomes Relativization ; otherwise it becomes complementation. 3) As nominal is used as function of Determinatives nominal is composed of Determinative marker {의}{φ} The ambiguti of determinative marker {의}is explained elearly according to the main verb of Constituent Sentence in deep structure.

파킨슨씨병 환자의 전두엽 기능 장애에 관한 연구

홍양의,이애영,김재문 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2

大學校 敎職員의 健康實態에 관한 基礎的 調査硏究

양점홍,황철문,남태호,백영호,윤양진,황영성 부산대학교 사범대학 1993 교사교육연구 Vol.26 No.-

The purpose of this study was to analyze the staffs' health of P. University through survey research. Also, the staffs's health problem was analyzed by the regular health test's result and their lifestyle. The results were as following: 1. The analysis of lifestyle (1) A habit of smoking 1. It was presented the male smokers(55.9%) and females smokers(0.6%) of P. university staffs were found in this study. 2. It was presented the male smokers smoked average 18.2 pieces a day. Also, they began to smoke cigarette 21.5 years old. 3. The persistent smokers were 24.6% and 52.2% of smokers be going to quit smoking cigarette someday in the future. (2) A habit of drinking 1. It was presented 85.7% of males and 59.1% of females subject drinking normally. 2. It was presented 3.7% of male subjects were drinking alcohol almost everyday in a week. 3. The most frequent kind of alcohol is as following order a beer(males 67.6% & females 79.6%) and a soju(males 28.3% & females 4.3%). Males drunken average amount of beer was 2 bottles(640ml) and females drunken average amount of beer was 0.8 bottles in a week. (3) A habit of exercise 1. It was presented males (29.0%) and females (15.1%) were exercising regularly, but males (23.3%) and females (38.6%) of staffs were not exercise at all. 2. It was found males' regular kind of exercise was as following order mountain-climbing, tennis, and jogging and females' regular kind of exercise was as order swimming, free handing exercise, and mountain-climbing. 3. It was presented 25.1% of staffs exercised 6 times a week regularly. And each times of exercise, 37.3% of staffs spent less than 30 minutes. 4. It was presented staffs exercised for health (72.4%) and 48.4% of staffs could not exercise as following reasons of not enough times, home work, research.... 5. Male staffs prefer as following order kind of sport tennis, swimming, and golf and female staffs prefer as following order kind of sport swimming, tennis, and bowling in the future. (4) A habit of eating 1. It was presented 5.5% of males and 16.6% of females did not have a breakfast regularly. 2. Concerning balance of food content: do not concerning food (males 34.7% & females 23.8%) and subjects like vegetables 9males 25.7% & females 32.7%) were found. 3. It was presented 8.5% of males and 5.4% of females took the vitamin regularly. (5) Health condition 1. In present evaluation for subject's health condition, very healthy (males 7.5% & females 6.0%) and poor health (males 2.0% & females 1.8%) were found in this study. 2. In present evaluation criterion for subject's health condition, the ability of adaptation on fatigue was appeared the most (males 35.9% & females 40.7%). 3. It was presented subjects have a digestive trouble (males 23.5% & females 33.1%) which were the best health problem in subjects. 2. A analysis of result of regular health test (1) Physique test 1. It was presented height (males 169.5cm & females 158.6cm) and body weight (males 65.9kg & females 53.1kg) were found in this study. 2. According to this study, obesity (males 11.2% & females 5.6%) and low body weight (males 8.8% & females 14.8%) were found in this study. 3. A high blood pressure subject (males 8.7% & females 2.5%) over than a contraction blood pressure 140mmHg and A high blood pressure subject (males 8.5% & females 1.9%) over than a expansion blood pressure 90mmHg were found in this study. (2) A blood test 1. Normal hemoglobin of subject (males 99.8% & females 62.3%) and 37.7% of females less than normal hemoglobin were found in this study. 2. 5.3% of males and 2.5% of females have greater than a concentration of glucose 140mg/dl, but there was anyone less than a concentration of glucose 50mg/dl. 3. The greater than 251mg/dl staff''s T.C.(males 7.1% & females 4.9%) and the less than 130mg/dl staffs' T.C.(males 2.0% & females 3.7%) were found in this study. 4. It was found males 5.3%, 11.6% & females 1.9%, 4.3% were presented greater than GOT and GPT 341U/L. (3) Hepatitis, Urinary Sugar, and Urinary Protein test 1. According to this study result, positive hepatitis was presented males (6.5%) and female(4.3%). 2. Urinary sugar is greater than +- males (1.8%) and females (2.4%) were found in this study. 3. Urinary protein is greater than +- males (1.6%) and females (1.8%) were found in this study. (4) Chest X-ray test 1. It was found male (1.6%) suffered from tuberculosis of the lungs. (5) Dental test 1. It was presented 77.3% of males and 88.9% of females was not existed the upper caries. Also, 75.7% of males and 90.7% of females was not existed the bottom caries. 2. It was presented 96.8% of males and 99.4% of females had the non-deficit of teeth in the upper part of teeth. Also, 93.9% of males and 98.1% of females had the non-deficit of teeth in the bottom part of teeth. 3. It was presented 19% of males and 3.7% of females have the periodontal disease. (6) General report and judgment 1. In general report, it was found 75.3% of males and 91.4% of females were the normal health condition. It was presented 9.1% of males and 4.9% of females were the liver disease, 4.9% of males and 1.2% of females were the sugar diabetes, 4.9% of males and 0.6% of females were the high blood pressure, and 1.4% of males and 1.9% of females were high arrest of bleeding. 2. In general judgment, it was presented 75.5% of males and 91.4% of females were the normal health condition. However, it was presented 17.8% of males and 7.4% of females were required high observation, 6.7% of males and 1.2% of females were required simple observation. As presented P. University staffs' lifestyle, it was found still many staffs have not right living habit. It is necessary to be wellness of lifestyle and satisfaction of life through correction of self responsibility and management in their life. Therefore, it is possible to live with healthy in better quality of life which is high quality of life and new concept of health were offered. Also, researcher expected the regular health examination be more practical contents than informal contents. Researcher recommended that it is necessary to support the university administration to be more flexibility of the regular health examination day than set the certain day of regular health examination and it required to create a way of using the P. university hospital.

한국산 꿩 Sertoli 세포의 계절적인 변화

양홍현,백영기,김인식 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.1

앤드류 잭슨의 국민중심주의와 주권론

양홍석 한국미국사학회 2003 미국사연구 Vol.17 No.-

In the development of American political history, it was in "the Age of Jackson" that, at first, the president was selected from western and ordinary people through "Convention," which contrasted the previous custom that symbolized eastern-oriented-elite group selected through undemocratic caucus. Also, it was in "the Age of Jackson" that the democratic spoils system, which used as "rotation in offices," put onto effect. The American historians interested in during 1830s have terms these reform movement as "Jacksonian Democracy." The historians have looked upon Andrew Jackson, as a States' rightist and egalitarian republican. And so, in the course of evolution of important events during the age of Jackson, I would like to analyze the exact meaning about "Jacksonian Democracy." On the basis of the his manuscripts at that time, I came to the conclusion that Jackson thought the voice of the people as a supreme law. Although the President was a man of powerful prejudices and passions, he identified his views with the voice of the people. Anyone who, he thought, crossed him was therefore an enemy of the sovereign people. To fulfill this voice of the people in the political area, he thought that the preservation of Stats' rights against Union's was indispensible precondition to achieve people-oriented democracy. He shared the strong faith in the self-reliance states-oriented republic community. And so, although the course of events during Jackson's term was erratic, but when his presidency was done, the federal authority was vast weaker, and the states, for practical purposes, were supreme.

심야전력을 이용한 축열식 전기온풍기 및 비축열식 전기패널 바닥난방의 실내온열환경 비교

양창남,임홍성,최동호 제주대학교 산업기술연구소 2000 尖端技術硏究所論文集 Vol.11 No.1

In this paper, we analyzed the characteristics of indoor heating environment by the electric panel heating system without thermal storage developed from the traditional under-floor heating system, and compared them with the ones by the electric heating system with thermal storage. From the experimental study, we observed little temperature difference of the upper and lower of space as well as the horizontal temperature distribution. The electric panel heating system makes the constant indoor temperature and more comfortable. However, we observed that the electric heating system with thermal storage is more economical than the electric panel heating system.

제주지역에서의 전기자동차 주행 특성

양원호,김홍석,현명택 제주대학교 공과대학 첨단기술연구소 2004 尖端技術硏究所論文集 Vol.15 No.2

Air pollution from auto-vehicle exhausted gas accounts for 70% of entire air pollution in Jeju. The introduction of electric vehicles which do not generate discharge emissions will improve the clean image in Jeju and boost tour industry. Driving tests have been conducted on the 5·16 and Ilju Roads to investigate the driving characteristics of electric vehicles, using Santa Fes provided by HyunDai Motor Co. The state of charging battery decreases rapidly on the uphill road of 546 Road, while it shows linear decreases on the flat road of Ilju Road. It is required to improve the charging ability of battery to adopt the electrical vehicle in Jeju.

장애학생을 위한 특수체육학급 설치에 관한 조사연구

홍양자,최인옥 한국특수체육학회 2003 한국특수체육학회지 Vol.11 No.2

The purpose of this study was to determine the current status of the establishment adapted physical education class for students with disabilities who cannot participate normal physical class and to give the research data by surveying the attitudes of the administrators, teachers and parents about adapted physical education class. the survey was executed the by teacher, normal teacher, administrator, and parents to research their attitudes towards the establishment of adapted physical education class. The finding are as follows: First in the case of a school which has no special class, the result of the survey was affirmative about the establishment of a special class. This result means that the interest and concern for the students with disabilities is higher. Second, concerning the necessity of the adapted physical education class, the attitude of the administrator, teacher and parents were very affirmative. Third, the physical teacher thought the students with disabilities to be insufficient in activity ability but to could do better through adapted physical education. Consequently, in school and home, positive concern and consideration help students with disabilities to grow up like normal children. First of all, adapted physical class should be established to develope physical education of special class.

여성 요실금에서 Kontinence™와 Compact Elite™의 치료효과 비교

양상국,정상원,오미희,노용수,김홍섭,우응제,박승훈,이수열 건국대학교 의과학연구소 1999 건국의과학학술지 Vol.9 No.-

Biofeedback and pelvic floor electrical stimulation are new modalities that have been advocated for the treatment of female urinary incontinence. The aim of this study was to compare prospectively the therapeutic effect of Kontinence™(HMT Co., Korea) with Compact Elite™(ECL Co., France) in female urinary incontinence. All patients were evaluated with a complete history, physical examination, urinalysis, urine culture and urodynamic study. Eighteen patients were treated with Compact Elite™, and thirteen patients with Kontinence™. Enrolled patients had completed 8 office sessions consisting of biofeedback and intravaginal electrical stimulation according to the program of each machines. Overall treatment response was graded by patients on a scale of complete cure to aggravation. At two weeks after last session, overall improvement rate was 78% in Compact Elite™group and 92% in Kontinence™group(p>0.05). Overall patients' acceptability during treatment course was greater than 80% in both machines(p>0.05). There were no unexpected clinical findings during Kontinence™ treatment. After Kontinence™treatment, there were no laboratory changes including electrocardiography, complete blood count, liver function profile, and chest PA. These results showed that the biofeedback and electrical stimulation with Kontinence™was safe and moderately effective for female urinary incontinence comparable to Compact Elite™. However, long-term results of a larger patients are needed to ensure the therapeutic effect of Kontinence™.