Combined Gene Therapy with Hypoxia-Inducible Factor-1α and Heme Oxygenase-1 for Therapeutic Angiogenesis

Bhang, Suk Ho,Kim, Ju Hee,Yang, Hee Seok,La, Wan-Geun,Lee, Tae-Jin,Kim, Ga Hee,Kim, Hyun Ah,Lee, Minhyung,Kim, Byung-Soo Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.7

<P>Transfection with either hypoxia-inducible factor-1α (HIF-1α) or heme oxygenase-1 (HO-1) gene can induce neovascularization in ischemic tissues. Although expression of transfected HIF-1α gene occurs rapidly, the expressed HIF-1α protein degrades quickly, limiting its therapeutic efficacy. Meanwhile, expressed HO-1 protein does not rapidly undergo degradation, but gene expression occurs a couple of days after transfection, resulting in apoptosis and a delay in angiogenesis in ischemic tissues at the incipient period of HO-1 gene transfection. We hypothesize that combined delivery of HIF-1α and HO-1 gene will enhance antiapoptosis and neovascularization in ischemic tissue compared with HIF-1α or HO-1 single-gene therapy. To test this hypothesis, ischemic mouse hindlimbs were treated with HIF-1α and/or HO-1 gene therapy. The combined gene therapy proved superior to both single-gene therapies, resulting in rapid expression of HIF-1α gene and long-term maintenance of expressed HO-1 protein. The apoptosis in the ischemic region was significantly less, and angiogenic growth factor secretion and angiogenesis were greater in the combined gene therapy than in either of the single-gene therapies. Our results suggest that a combined gene therapy of HIF-1α and HO-1 enhances the transfection of both genes and improves angiogenesis compared with either single-gene therapy.</P>

소음 측정을 통한 에어컨 실내기 자기음 검출 및 진단

지효근,정호제,김정현 경성대학교 공학기술연구소 2012 공학기술연구지 Vol.18 No.-

This paper presents the detection and diagnosis of air-conditioner electromagnetic sound through noise measurements. Electromagnetic sound originating from the motor is an unpleasant source of unwanted noise that should be detected at the manufacturing stage. A detection system using sound measurements was built and a detection algorithm based on FFT analysis is presented. Soun measurements are preferable over direct vibration measurement because it is non-contact and low cost. Experimental results show that our sound measurement system can detect electromagnetic sound effectively compared to using vibration measurements.

중첩 방전을 이용한 CO₂레이저의 동작 특성 : 다중펄스 중첩을 이용한 long 펄스형 CO₂레이저의 펄스 형상 제어 Pulse shape control of pulse CO₂laser using multiple-pulse superposition

정현주,김근용,정용호,정종한,송건주,김희제 부산대학교 생산기술연구소 2001 生産技術硏究所論文集 Vol.60 No.-

본 연구에서는 PIC 원 칩 마이크로 프로세서 기술을 접목시켜, 저전압 측에서 2단 또는 3단의 회로를 능동적으로 중첩시킴으로써, 다양한 펄스폭을 가지는 고전압의 펄스형상을 만들었다. 이렇게 만들어진 다양한 중첩펄스를 현재 산업용 및 의료용으로 많이 사용되고 있는 CO2 레이저에 적용하여 각 단의 스위치의 트리거 지연시간에 따른 레이저 빔의 출력 특성을 고찰하였다. 그 결과 다양한 펄스폭을 가지는 레이저 빔을 얻을수가 있었고 특히, 3단 중첩회로에서 스위칭 지연 시간을 변화시킴에 따라 얻어진 레이저 펄스폭은 최소 약 4ms였고 최대 약 10ms였다. In this study, a variable pulse length, high duty cycle Pulse Forming Network(PFN) is constructed by time sequence. The power supply for this experiment consists of three switching circuits. Each switching circuit uses 2 mesh PFN, a charging circuit, a HV leakage transformer, and bridge rectifier on each transformer secondary. The PFN elements operate at low voltage and drive the primary of HV leakage transformer. The secondary of the transformer has a full-wave rectifier, which passes the pulse energy to the load in a continuous sequence. We investigated laser pulse width of pulsed CO2 laser as various delay time among three switching circuits. As a result, we can obtain various laser pulse width from about 4 to 10ms.

Global-MAHN에서 라우팅 오버헤드 개선에 대한 연구

張良根,金玄旭,李光培,吳世德,裵振勝,金在浩 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-

In this Paper, we studied the mechanism that increases the network reliability and decreases network load in GMAHN environment based on the AODV routing protocol. This proposed algorithm is the research on the algorithm, minimizing network overhead by preventing unnecessary network overhead previously and adapting Local repair mechanism to efficient data transmission when route error happens in network, in GMAHN environment. To evaluate the proposed algorithm's performance, we analyzed the proposed algorithm and adapting in various experiment environment according to 5 experiment viewpoint such as node movement, gateway network zone, mobile node number, flow number. After evaluating the experiment, we confirmed better network improvement altogether through following result such as total average data reception rate's increase, average data transmission time's decrease, network overhead's decrease than existing GMAHN algorithm.

고성능 표면탄성파 필터의 최적 설계에 관한 연구

신양호,김귀현,이진복,엄현석,이중근,박진석 漢陽大學校 工學技術硏究所 1999 工學技術論文集 Vol.8 No.1

본 논문에서는 고성능 표면탄성파 필터의 최적 설계를 위하여 다양한 IDT(interdigital transducer) 구조의 필터를 제작하고 각각의 주파수 응답특성을 비교 분석하였다. 기본 구조외에 MSC(micro strip-coupler), double split, reflector, ground line 등의 구조를 일반적으로 널리 사용되고 있는 Y-Z cut l-N(LiNbO3) 기판 위에 형성시켰다. 제작된 소자의 중심주파수, 통과 대역, 삽입손실등을 측정하였으며, 주파수 응답에 기초한 기존 모델의 효용성을 논의하였다. Effects of IDT structures on frequency responses of SAW filters are investigated to achieve a high performance SAW filter, Various types of IDTS, such as MSC(multistrip-coupler), double split, reflector, and ground line, are designed and patterned on a widely used Y-Z cut LN(LiNbO3) substrate. Center frequencies, band widths, and insertion losses are measured and compared. Invalidity of the conventional model based on the impulse response model is also discussed.

UV 및 젖산 처리에 의한 난각 표면의 Salmonella 억제 효과

손원근,이철현,강호조 한국수의공중보건학회 2002 예방수의학회지 Vol.26 No.4

The inhibitory effects were investigated of 254-nm ultraviolet (UV) radiation and lactic acid (LA) treatment on populations of Salmonella (S) enteritidis on eggshell surface. All UV exposures (730, 1,580, 3,000 W/cm²) for 30 sec significantly reduced S enteritidis (P 0.05). UV irradiation with 3,000 μW/cm² almost completely eliminated S enteritidis on inoculated eggshells by 99%. These results suggest that the higher intensity of UV irradiation may be associated with the higher germicidal activity. However, no significant differences were detected between UV exposure times. The treatments of LA at the concentration of 0.1, 0.3 and 0.5 % also specifically decreased the bacterial cell populations (0.91 1.14 log CFU) on the eggshell compared with the control (6.92 log CFU). The treatment of LA at the concentration of 0.5 % had the reaction of a 2.52 log unit storage for 9 days. The combined treatment of UV (3,000 μW/cm²) and LA(0.5%) decreased S enteritidis populations on eggshell by 3.85 log unit, compared wth the control (P 0.05), and its inhibitory effect was stronger with storage time.

물-공기 계면에서 긴 사슬 양 말단 작용기 분자인 α,ω-13,16-Dimethyloctacosanedioate Dimethylester(C_30DME)의 거동에 관한 연구

이정근,손대원,이관수,송석호,김현명,정선호 한양대학교 자연과학연구소 2001 自然科學論文集 Vol.20 No.-

물-공기 계면에서 표면압력의 증가에 따른 C30DME 분자의 구조 변화를 살펴보았다. 표면압력(π)-표면적(A) 등온선을 통해 표면적이 줄어들 때 C30DME 분자가 선형으로부터 역 U 자형 구조로 변하는 것을 확인하였다. C30DME의 역 U 자형 구조의 형성을 확인하기 위하여 Surface Plasmon Resonance (SPR)와 ellipsometry를 이용하여 Langmuir-Blodgett (LB) film 의 두께를 측정하였고 이 두께는 C30DME 분자의 역 U 자형 구조의 높이와 일치함을 관찰하였다. 또한 Atomic Force Microscope (AFM)을 이용한 표면 거침도 측정의 결과는 선형으로부터 역 U자형 구조로의 구조적 변화가 불연속이라는 것을 보여주었다. We investigated the conformational change of C30DME molecule with an increase on surface pressure at the air-water interface. The surface pressure (π)-area (A) isotherm indicates that the structure of C30DME changed from linear to reverse U-shape as the surface area was compressed. In order to confirm the formation of reverse U-shape, we examined the thickness of Langmuir-Blodgett (LB) film by a surface plasmon resonance (SPR) and an ellipsometry. The result of surface roughness measurement using an atomic force microscope (AFM) showed that the conformational change from linear to reverse U-shape is discontinuous.

이단하소에 의해 제조된 PNN-PZ-PN 세라믹의 압전 및 유전특성

이수호,손무헌,조현철,김한근,사공 건 東亞大學校 1997 東亞論叢 Vol.34 No.-

In the fields of the optics, precise machine, semiconductors, the micro-positioning actuators are required for the control of position in submicron range. PNN-PZN-PZT cermics were fabricated with various mole ratio of the PZT[Pb(Zr½ Ti½)O₃] PNN[Pb(Ni⅓Nb⅔)O₃]and PZN[Pb(Zn⅓Nb⅔)O₃]powders prepared by double calcination and PZT powders prepared by molten-salt synthesis method. The formation rate of perovskite phase in PNN-PZN-PZT ceramics could be obtained about 92% at PZT 0.3 mole ratio. The relative permittivity of specimen with PZT 0.3 mole ratio was shown 5,320 and appeared the relaxor ferroelectric feature. The maximum piezoelectric coefficient d₃₁to be used for evaluation the displacement of piezoceramics in PNN-PZN-PZT ceramics was 324×10¹²(C/V) at the vicinity of morphotropic phase boundary and was larger than that of solid PZT ceramics(120×10-¹²C/V).

GIS 기반의 유역물수지 분석모형 개발

황의호,김계현,박진혁,이근상 한국지리정보학회 2004 한국지리정보학회지 Vol.7 No.4

기존 새만금유역의 물수지분석은 유역전체를 단일유역으로 단순화하여 가지야마식으로 산출한 유출량을 기초로 행해졌으나, 새만금유역은 용수공급체계가 복잡 다양하고 향후 임의의 지점에서물수지요소가 변경될 때 탄력적으로 적용하기엔 어려움이 있다 본 연구에서는 새만금유역에 적합한 물수지분석모형 적용을 위하여 수개의 물수지 단위소유역으로 구분하고 각각의 소유역별 수자원의 개발과 이용에 대해 물수지를 상세분석할 수 있는 GIS 기반의 유역물수지모형을 개발하였다. 유역의 물수지 상세 분석을 위하여 UML 다이어그램을 이용한 물수지모형의 추상 및 개년 설계, 용수공급체계 반영을 위한 하천망 구성, GIS 기반의 전처리 및 후처리 모듈 개발, 모형 보정 및 검증 등을 포함하는 연구를 수행하였다. 이러한 물수지분석모형은 수자원의 유출과정 해석을 통해 유역내 효율적인 수자원관리 방안을 수립할 수 있도록 지원이 가능하며, 사웅자로 하여금 유역의체계적인 수자원관리에 있어 수작업을 최소화하고 다원화된 모델 분석체계를 지원함으로써 보다 편리하고, 합리적인 수자원관리방안 수립에 지원이 가능할 것으로 사료된다. Existing Semangeum's water balance analysis simplifies whole basin to single basin and achieved volume of effluence that produce by Kajiyama way to foundation. But Semangeum is complicated and various rice-wine strainer supply system. And there is difficulty to apply as elastic when water balance element is changed at free point. Divided to unit possession station for suitable water balance analysis model application to Semangeum in this study. And developed basin water balance model of GIS base that can do details analysis is bite about development and transfer of an appropriation in the budget of basin water resources. Achieved study including abstraction and concept design that use UML (unified modeling language) diagram for details analysis, stream network composition for rice-wine strainer supply system application, preprocessing of GIS base and postprocessing module development, model revision and verification etc. Support of this water balance analysis model is available to establish efficient water resources administration plan through outward flow process analysis of water resources. And support is considered to be possible in more convenient and, reasonable water resources administration way establishment by minimizing manual processing in systematic water resources government official to user and support diversified analysis system.

GMAHN 환경에서의 TCP 프로토콜 성능 분석

吳世德,金玄旭,李光培,張良根,裵振勝,金在浩 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-

Recently, GMAHN that provides interface between MANET and Wired Network has been focused in Mobile Communication. It is necessary that The technology provide reliable data transmission technology between mobile node and wired network in MANET environment that varing by the node movement. In this paper, using the TCP protocol(Tahoe, Reno, Vegas, SACK)that increases reliability between source and destination, we applied the TCP protocol mechanism to various environment, and compared result, and also proposed the efficient TCP mechanism by comparing each mechanism.